细胞因子信号转导抑制蛋白-3(suppressor of cytokine signaling,SOCS-3)是一种细胞因子信号转导阻抑蛋白,参与体内多种信号转导通路的调节。研究发现,SOCS-3与肝纤维化、病毒性肝炎和肝肿瘤的发生发展均存在一定的关系,并且干扰素的抗...细胞因子信号转导抑制蛋白-3(suppressor of cytokine signaling,SOCS-3)是一种细胞因子信号转导阻抑蛋白,参与体内多种信号转导通路的调节。研究发现,SOCS-3与肝纤维化、病毒性肝炎和肝肿瘤的发生发展均存在一定的关系,并且干扰素的抗病毒疗效与SOCS-3的遗传学表达改变密切相关。本综述将对SOCS-3的结构、分子功能及近年来在肝疾病中的相关研究进行简要总结。展开更多
SOCS(suppressor of cytokine signaling)蛋白是通过Janus激酶(JAK)/信号转导与转录激活子(STAT)途径激活的细胞因子信号转导的一个重要的生理抑制剂家族,SOCS-3是该家族成员之一,主要参与负调控生长激素、白细胞介素(IL)-1I、L-2I、L-...SOCS(suppressor of cytokine signaling)蛋白是通过Janus激酶(JAK)/信号转导与转录激活子(STAT)途径激活的细胞因子信号转导的一个重要的生理抑制剂家族,SOCS-3是该家族成员之一,主要参与负调控生长激素、白细胞介素(IL)-1I、L-2I、L-6、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、催乳素等细胞因子的信号转导。最近研究发现,SOCS-3在胰岛素信号转导中有十分重要的调控功能,本文对其在胰岛素抵抗发生中作用的研究现状作一综述。展开更多
细胞因子信号抑制物（suppressor of cytokine signaling,SOCS）家族是具有调控Janus激酶/信号转导与转录激活因子（janus kinase/signal transducer and activator of transcription,JAK/STAT）信号通路作用的蛋白质家族,包括SOCS-1-7...细胞因子信号抑制物（suppressor of cytokine signaling,SOCS）家族是具有调控Janus激酶/信号转导与转录激活因子（janus kinase/signal transducer and activator of transcription,JAK/STAT）信号通路作用的蛋白质家族,包括SOCS-1-7及CIS。其中,SOCS-3对胰岛素信号转导具有重要调节作用,通过调控胰岛素受体、胰岛素受体底物、JAK 2、STAT 3、瘦素等信号转导因子,介导胰岛素抵抗的发生和发展。SOCS-3有望成为治疗胰岛素抵抗新的作用靶点。展开更多
Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (R...Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There were no significant differences between the EA group and the drug group (all P>0.05). Conclusion: EA can alleviate the inflammatory response of RA rats, improve their pathological conditions, reduce the expressions of phospho-JAK2 and phospho-STAT3 in the synovial tissue of knee joint, and decrease the expressions of JAK2 and STAT3 mRNAs. The therapeutic effect of EA is comparable to that of the tripterygium glycosides. The mechanism of EA treatment may be related to the inactivation of the JAK2/STAT3 pathway.展开更多
细胞因子信号抑制蛋白（suppressors of cytokinesignaling,SOCS）是由3个研究小组分别采用不同的方法发现的,也称为细胞因子信号转导抑制因子。它主要通过抑制JAK/STAT通路对细胞因子进行负反馈调节,被称为细胞的“分子刹车”。SOCS家...细胞因子信号抑制蛋白（suppressors of cytokinesignaling,SOCS）是由3个研究小组分别采用不同的方法发现的,也称为细胞因子信号转导抑制因子。它主要通过抑制JAK/STAT通路对细胞因子进行负反馈调节,被称为细胞的“分子刹车”。SOCS家族包括SOCS-1、SOCS-2、SOCS-3、CIS（cytokine—inducible SH2-containing protein）以及SOCS-4-SOCS-7。展开更多
文摘细胞因子信号转导抑制蛋白-3(suppressor of cytokine signaling,SOCS-3)是一种细胞因子信号转导阻抑蛋白,参与体内多种信号转导通路的调节。研究发现,SOCS-3与肝纤维化、病毒性肝炎和肝肿瘤的发生发展均存在一定的关系,并且干扰素的抗病毒疗效与SOCS-3的遗传学表达改变密切相关。本综述将对SOCS-3的结构、分子功能及近年来在肝疾病中的相关研究进行简要总结。
文摘SOCS(suppressor of cytokine signaling)蛋白是通过Janus激酶(JAK)/信号转导与转录激活子(STAT)途径激活的细胞因子信号转导的一个重要的生理抑制剂家族,SOCS-3是该家族成员之一,主要参与负调控生长激素、白细胞介素(IL)-1I、L-2I、L-6、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、催乳素等细胞因子的信号转导。最近研究发现,SOCS-3在胰岛素信号转导中有十分重要的调控功能,本文对其在胰岛素抵抗发生中作用的研究现状作一综述。
文摘细胞因子信号抑制物（suppressor of cytokine signaling,SOCS）家族是具有调控Janus激酶/信号转导与转录激活因子（janus kinase/signal transducer and activator of transcription,JAK/STAT）信号通路作用的蛋白质家族,包括SOCS-1-7及CIS。其中,SOCS-3对胰岛素信号转导具有重要调节作用,通过调控胰岛素受体、胰岛素受体底物、JAK 2、STAT 3、瘦素等信号转导因子,介导胰岛素抵抗的发生和发展。SOCS-3有望成为治疗胰岛素抵抗新的作用靶点。
文摘Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There were no significant differences between the EA group and the drug group (all P>0.05). Conclusion: EA can alleviate the inflammatory response of RA rats, improve their pathological conditions, reduce the expressions of phospho-JAK2 and phospho-STAT3 in the synovial tissue of knee joint, and decrease the expressions of JAK2 and STAT3 mRNAs. The therapeutic effect of EA is comparable to that of the tripterygium glycosides. The mechanism of EA treatment may be related to the inactivation of the JAK2/STAT3 pathway.
文摘细胞因子信号抑制蛋白（suppressors of cytokinesignaling,SOCS）是由3个研究小组分别采用不同的方法发现的,也称为细胞因子信号转导抑制因子。它主要通过抑制JAK/STAT通路对细胞因子进行负反馈调节,被称为细胞的“分子刹车”。SOCS家族包括SOCS-1、SOCS-2、SOCS-3、CIS（cytokine—inducible SH2-containing protein）以及SOCS-4-SOCS-7。
