期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Molecular characterization of two suppressor of cytokine signaling 1 genes(SOCS1a and SOCS1b) in chickens
1
作者 Xue XU Jiannan ZHANG +1 位作者 Juan LI Yajun WANG 《Frontiers of Agricultural Science and Engineering》 2015年第1期73-83,共11页
Suppressor of cytokine signaling 1(SOCS1)protein can inhibit the signal transduction triggered by some cytokines or hormones and thus are important in many physiological/pathological processes, including innate and ad... Suppressor of cytokine signaling 1(SOCS1)protein can inhibit the signal transduction triggered by some cytokines or hormones and thus are important in many physiological/pathological processes, including innate and adaptive immunity, inflammation, and development in mammals. However, there is sparse information about their structure, tissue expression, in birds, where their biological functions remain unknown. In this study,we cloned and characterized two SOCS1 genes(named c SOCS1 a and c SOCS1b) from chickens. SOCS1 a is predicted to encode a 207-amino acid protein, which shares high amino acid sequence identity(64%–67%) with human and mouse SOCS1. Besides SOCS1 a, a novel SOCS1 b gene was also identified in chickens and other non-mammalian vertebrates including Xenopus tropicalis.Chicken SOCS1 b is predicted to encode a 212-amino acid protein, which shares only 30%–32% amino acid sequence identity with human SOCS1 and c SOCS1 a. RT-PCR assay revealed that both c SOCS1 a and c SOCS1 b are widely expressed in all chicken tissues. Using a luciferase reporter assay system, we further demonstrated that transient expression of c SOCS1 a and c SOCS1 b can significantly inhibit chicken growth hormone(GH)- or prolactin(PRL)-induced luciferase activities of Hep G2 cells expressing c GH receptor(or c PRL receptor), indicating that SOCS1 a and SOCS1 b proteins can negatively regulate GH/PRL signaling. Taken together, these data suggest that both c SOCS1 a and c SOCS1 b may function as negative regulators of cytokine/hormone actions, such as modulation of GH/PRL actions in chickens. 展开更多
关键词 CHICKEN SOCS1a socs1b growth hor-mone PROLACTIN
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部