[Objective] This study aimed to revealing the effects of two kinds of heavy metals including Cr6+ and Pb2+ on garlic germination.[Method] Root length,enzyme(SOD and CAT)activity and soluble protein content during ...[Objective] This study aimed to revealing the effects of two kinds of heavy metals including Cr6+ and Pb2+ on garlic germination.[Method] Root length,enzyme(SOD and CAT)activity and soluble protein content during garlic germination were determined under the stress of two kinds of heavy metals including Cr6+ and Pb2+.[Result] During garlic germination,there was an obvious inhibition on buds and seedlings when the concentration of heavy metals including Cr6+ and Pb2+ was more than 20 mg/L,while SOD and CAT had certain buffering ability to the heavy metal toxicity with low concentration.With the stress of Cr6+ in low concentration,SOD activity increased with the increase of metal ion concentration,and then reached maximum when the treatment concentration was 50 mg/L,while CAT activity gradually increased when Cr6+ concentration was lower than 20 mg/L.With the high concentration of Cr6+(more than 50 mg/L)or the Pb2+ stress,SOD and CAT activity continued to decrease with the increase of concentration of heavy metal ions.With various concentrations of heavy metal(Cr6+ and Pb2+)solution,soluble protein content showed the decreasing trend with time prolong,and the decreasing trend was obvious with the increase of concentration.Moreover,the tolerance of garlic on Cr6+ toxicity was much higher than that on Pb2+.[Conclusion] Two kinds of heavy metals including Cr6+ and Pb2+ have impacts on four factors mentioned above during garlic germination,and this study provides a theoretical basis for further discussion of the toxic mechanism of heavy metals on plants.展开更多
基金Supported by Key Project of Haixi Construction Served by Universities in Fujian(A101)Subject(Biology)Determined and Constructed by Master Degree-conferring Units+1 种基金A-class Scientific and Technological Project of Education Department in Fujian(JA09213)Scientific and Technological Project of Quanzhou(2010N28)~~
文摘[Objective] This study aimed to revealing the effects of two kinds of heavy metals including Cr6+ and Pb2+ on garlic germination.[Method] Root length,enzyme(SOD and CAT)activity and soluble protein content during garlic germination were determined under the stress of two kinds of heavy metals including Cr6+ and Pb2+.[Result] During garlic germination,there was an obvious inhibition on buds and seedlings when the concentration of heavy metals including Cr6+ and Pb2+ was more than 20 mg/L,while SOD and CAT had certain buffering ability to the heavy metal toxicity with low concentration.With the stress of Cr6+ in low concentration,SOD activity increased with the increase of metal ion concentration,and then reached maximum when the treatment concentration was 50 mg/L,while CAT activity gradually increased when Cr6+ concentration was lower than 20 mg/L.With the high concentration of Cr6+(more than 50 mg/L)or the Pb2+ stress,SOD and CAT activity continued to decrease with the increase of concentration of heavy metal ions.With various concentrations of heavy metal(Cr6+ and Pb2+)solution,soluble protein content showed the decreasing trend with time prolong,and the decreasing trend was obvious with the increase of concentration.Moreover,the tolerance of garlic on Cr6+ toxicity was much higher than that on Pb2+.[Conclusion] Two kinds of heavy metals including Cr6+ and Pb2+ have impacts on four factors mentioned above during garlic germination,and this study provides a theoretical basis for further discussion of the toxic mechanism of heavy metals on plants.