Objective To explore the effects of different acupuncture-moxibustion methods on the plasma substance P（SP） content,and the expression of colonic SP m RNA of the rats with functional constipation.Methods Sixty healt...Objective To explore the effects of different acupuncture-moxibustion methods on the plasma substance P（SP） content,and the expression of colonic SP m RNA of the rats with functional constipation.Methods Sixty healthy male SD rats were divided into six groups according to the random number table,including blank control group（group B,n=8）,model group（group M,n=11）,drug control group（group D,n=8）,filiform needle group（group F,n=11）,electroacupuncture group（group E,n=11） and moxibustion group（group MB,n=11）.The rats in group B and group M accepted no intervention,gavage with cisapride suspension was conducted in group D,and electroacupuncture,filiform needling and moxibustion therapies were carried out in group E,group F and group MB for 6 days.After treatment,the stool quantity and fecal moisture content during 24 h of the rats were observed in each group,colon tissue structure and the acidic mucus in mucosa layer were observed,plasma SP content was detected and the expression of colonic SP m RNA was detected.Results compared with group B,the stool quantity and fecal moisture content during 24 h of the rats in group M decreased significantly（P0.01）;compared with group M,the stool quantity during 24 h of the rats in each group increased（P0.05 or 0.01）,and the fecal moisture content during 24 h of the rats in each group also increased significantly except group MB（P0.01）;compared with group D,the fecal moisture content during 24 h of the rats in group MB decreased（P0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,there was no difference in the stool quantity during 24 h of the rats in each group（P0.05）,the fecal moisture contents during 24 h of the rats in group F and group E were higher than that of group MB（P0.01）,and there was no difference between group F and group E（P0.05）.HE staining of colon tissue of rats showed that the structure was normal and complete,and there was no difference among each group.PAS staining showed that the acidic mucus level of the rats in group M decreased significantly when compared with group B;compared with group M,the acidic mucus level of the rats in group D,fgroup F,group E and group MB increased in varying degrees.Compared with group B,the plasma SP content of the rats in group M decreased（P0.05）;compared with group M,the plasma SP contents of the rats in group F and group MB increased（P0.01）;the plasma SP contents of the rats in group F,group MB and group E were higher than that of group D（P0.05 or 0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,the plasma SP content of the rats in group F was higher than that of group MB and electro-acupuncture group（P0.05）,and there was no difference between group MB and group E.Compared with group B,the expression level of colonic SP m RNA of the rats in group M elevated（P0.05）;compared with group M,the expression level of colonic SP m RNA of the rats in the four intervention groups declined（P0.05）;compared with drug group D,there was no difference among group F,group E and group MB（P0.1）;according to the comparison among the groups with different intervention methods,the expression level of colonic SP m RNA of the rats in group MB was lower than that of group F（P0.05）.Conclusion The different efficacy of different acupuncture-moxibustion methods in treatment of functional constipation may be related with the mechanism of regulation of plasma SP content and the expression level of colonic SP m RNA.展开更多
In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural...In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by immunohistochemically measured. The mRNA expression in the lung tissues was determined by reverse transcription polymerase chain reaction （RT-PCR）. The number of cells positive for SPA of the S group （0.52±0.05） was lower than that of the N group （0.72±0.06） （P〈0.05）. The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease.展开更多
基金Supported by Young Talents Program of Hebei Higher Education Institutions:BJ2014047Scientific Research Fund Project for Doctors of Hebei University of Chinese Medicine:BSZ2015009National Natural Science Foundation
文摘Objective To explore the effects of different acupuncture-moxibustion methods on the plasma substance P（SP） content,and the expression of colonic SP m RNA of the rats with functional constipation.Methods Sixty healthy male SD rats were divided into six groups according to the random number table,including blank control group（group B,n=8）,model group（group M,n=11）,drug control group（group D,n=8）,filiform needle group（group F,n=11）,electroacupuncture group（group E,n=11） and moxibustion group（group MB,n=11）.The rats in group B and group M accepted no intervention,gavage with cisapride suspension was conducted in group D,and electroacupuncture,filiform needling and moxibustion therapies were carried out in group E,group F and group MB for 6 days.After treatment,the stool quantity and fecal moisture content during 24 h of the rats were observed in each group,colon tissue structure and the acidic mucus in mucosa layer were observed,plasma SP content was detected and the expression of colonic SP m RNA was detected.Results compared with group B,the stool quantity and fecal moisture content during 24 h of the rats in group M decreased significantly（P0.01）;compared with group M,the stool quantity during 24 h of the rats in each group increased（P0.05 or 0.01）,and the fecal moisture content during 24 h of the rats in each group also increased significantly except group MB（P0.01）;compared with group D,the fecal moisture content during 24 h of the rats in group MB decreased（P0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,there was no difference in the stool quantity during 24 h of the rats in each group（P0.05）,the fecal moisture contents during 24 h of the rats in group F and group E were higher than that of group MB（P0.01）,and there was no difference between group F and group E（P0.05）.HE staining of colon tissue of rats showed that the structure was normal and complete,and there was no difference among each group.PAS staining showed that the acidic mucus level of the rats in group M decreased significantly when compared with group B;compared with group M,the acidic mucus level of the rats in group D,fgroup F,group E and group MB increased in varying degrees.Compared with group B,the plasma SP content of the rats in group M decreased（P0.05）;compared with group M,the plasma SP contents of the rats in group F and group MB increased（P0.01）;the plasma SP contents of the rats in group F,group MB and group E were higher than that of group D（P0.05 or 0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,the plasma SP content of the rats in group F was higher than that of group MB and electro-acupuncture group（P0.05）,and there was no difference between group MB and group E.Compared with group B,the expression level of colonic SP m RNA of the rats in group M elevated（P0.05）;compared with group M,the expression level of colonic SP m RNA of the rats in the four intervention groups declined（P0.05）;compared with drug group D,there was no difference among group F,group E and group MB（P0.1）;according to the comparison among the groups with different intervention methods,the expression level of colonic SP m RNA of the rats in group MB was lower than that of group F（P0.05）.Conclusion The different efficacy of different acupuncture-moxibustion methods in treatment of functional constipation may be related with the mechanism of regulation of plasma SP content and the expression level of colonic SP m RNA.
基金grants from the National Natural Sciences Foundation of China (No. 30500224, 3040199)
文摘In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by immunohistochemically measured. The mRNA expression in the lung tissues was determined by reverse transcription polymerase chain reaction （RT-PCR）. The number of cells positive for SPA of the S group （0.52±0.05） was lower than that of the N group （0.72±0.06） （P〈0.05）. The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease.
