Based on the previous studies on numerical taxonomy, SDS\|PAGE of whole\|cell protein and DNA hybridization, the rhizobial strains isolated from \%Kummerowia\% sp. in semi\|arid area of North\|west constituted a new s...Based on the previous studies on numerical taxonomy, SDS\|PAGE of whole\|cell protein and DNA hybridization, the rhizobial strains isolated from \%Kummerowia\% sp. in semi\|arid area of North\|west constituted a new subgroup, the 16S rDNA sequence of representative strain SH714 were tested. The unrooted phylogenetic tree was produced. In this tree, the strain SH714 with \%Sinorhizobium xinjiangensis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% constituted a branch of \%Sinorhizobium.\% Within this branch, the similarity valuse of 16S rDNA sequence between strain SH714 and \%S. xinjiangesis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% were 97.4%, 97.5%, 96.8%, 96.7%, 97.2% and 95.6% respectively, the values were more than 95%, this indicated that these known species should belong to the same genus. The values of DNA homology between type strains of these species were less than 70%. Thus, the strain SH714 represented a new rhizobial species, and there were some diversity between SH714 and known rhizobial species in phenotypic feature and composition of protien.展开更多
文摘目的:探讨吸入药物联合无创正压通气(noninvasive positive pressure ventilation,NPPV)对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)合并呼吸衰竭患者营养状况及Clara细胞蛋白(CC16)、肺表面活性蛋白D(SP-D)表达的影响。方法:选取2018年1月-2019年1月于本院呼吸内科住院治疗的COPD合并呼吸衰竭患者50例,按照随机数字表法将其分为观察组和对照组,各25例。对照组吸入布地奈德福莫特罗吸入粉雾剂治疗,观察组在对照组基础上联合NPPV。比较两组治疗前后动脉血气指标、圣乔治呼吸问卷(St.George’s respiratory questionnaire,SGRQ)评分、改良英国医学研究学会呼吸困难指数(modified British medical research council,mMRC)、6 min步行试验(6-minute walk test,6-MWT)、营养指标、CC16及SP-D表达水平。结果:治疗后,观察组SGRQ评分与mMRC分级均低于对照组,而6-MWT高于对照组(P<0.05)。治疗后,观察组动脉血氧分压(PaO2),动脉血氧饱和度(SaO2)及氧合指数(OI)均高于对照组,而动脉二氧化碳分压(PaCO2)低于对照组(P<0.05)。治疗后,观察组体质指数(BMI)、血清总蛋白(TP)与血清白蛋白(ALB)水平均高于对照组(P<0.05)。治疗后,观察组CC16表达水平高于对照组,而SP-D表达水平低于对照组(P<0.05)。结论:吸入药物联合NPPV可有效改善COPD合并呼吸衰竭患者的症状与营养状况,增加CC16表达量、降低SP-D表达量,值得临床推广应用。
文摘Based on the previous studies on numerical taxonomy, SDS\|PAGE of whole\|cell protein and DNA hybridization, the rhizobial strains isolated from \%Kummerowia\% sp. in semi\|arid area of North\|west constituted a new subgroup, the 16S rDNA sequence of representative strain SH714 were tested. The unrooted phylogenetic tree was produced. In this tree, the strain SH714 with \%Sinorhizobium xinjiangensis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% constituted a branch of \%Sinorhizobium.\% Within this branch, the similarity valuse of 16S rDNA sequence between strain SH714 and \%S. xinjiangesis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% were 97.4%, 97.5%, 96.8%, 96.7%, 97.2% and 95.6% respectively, the values were more than 95%, this indicated that these known species should belong to the same genus. The values of DNA homology between type strains of these species were less than 70%. Thus, the strain SH714 represented a new rhizobial species, and there were some diversity between SH714 and known rhizobial species in phenotypic feature and composition of protien.
