AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were colle...AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups:benign ascites(n=21)and malignant ascites(n=15).Benign ascites included cirrhotic ascites(n=10)and tuberculous ascites(n=5).Malignant ascites included oophoroma(n=7),cancer of colon(n=5),cancer of the liver(n=6),gastric cancer(n=2),and bladder carcinoma(n=1).The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction(RT-PCR)technology,and the positive rate of COX-2 mRNA was compared between different diseases.RESULTS:The positive rate of COX-2 mRNA in malignant ascites was 42.9%(9/21),which was significantly higher than in benign ascites,6.7%(1/15),difference being significant between these two groups(χ2=4.051,P=0.044).The proportion of the positive rate in the malignant ascites was as follows:ovarian cancers57.1%(4/7),colon cancer 40.0%(2/5),liver cancer33.3%(2/6),gastric cancer 50.0%(1/2),and bladder cancer 0.00%(0/1).However,there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites(χ2=1.614,P=0.806).Among the benign ascites,COX-2 mRNA levels were different between the tuberculous ascites(0/5)and cirrhotic ascites(1/10),but there was no significant difference(P=1.000).CONCLUSION:COX-2 mRNA,detected by RT-PCR,is useful in the differential diagnosis of benign and malignant ascites,which also has potential value in the clinical diagnosis of tumors.展开更多
【目的】通过检测腹水COX-2 m RNA的表达水平,探讨COX-2 m RNA对腹水鉴别诊断的价值和意义。【方法】收集2014年6月至2015年2月期间到我院就诊并明确诊断的恶性腹水34例,良性腹水15例。采用实时荧光定量PCR方法检测腹水中COX-2 m RNA的...【目的】通过检测腹水COX-2 m RNA的表达水平,探讨COX-2 m RNA对腹水鉴别诊断的价值和意义。【方法】收集2014年6月至2015年2月期间到我院就诊并明确诊断的恶性腹水34例,良性腹水15例。采用实时荧光定量PCR方法检测腹水中COX-2 m RNA的表达水平,比较COX-2 m RNA在良恶性腹水中表达的差异,及在不同原发疾病中的表达情况。【结果】34例恶性腹水及15例良性腹水均扩增出COX-2,良恶性腹水中COX-2 m RNA的相对表达水平与性别、年龄构成比无关系,差异无统计学意义(P>0.05)。恶性腹水和良性腹水COX-2 m RNA的表达水平分别为7.93(5.04,16.58)和0.95(0.57,1.79),即恶性腹水COX-2 m RNA的表达水平显著高于良性腹水,差异有统计学意义(P<0.05)。恶性腹水COX-2m RNA的表达水平在不同原发疾病中差异无统计学意义(P>0.05),良性腹水COX-2 m RNA表达水平在肝硬化和结核性腹膜炎中分别为1.55(0.55,2.55)和0.87(0.61,1.38),差异无统计学意义(P>0.05)。【结论】COX-2 m RNA在恶性腹水中的表达水平显著高于良性腹水,在鉴别诊断腹水性质中具有重要的参考价值。展开更多
基金Supported by Fund of Science and Technology Plan Project in Zhuhai,No.PC20061084
文摘AIM:To investigate the mRNA expression of cyclooxygensae-2(COX-2)in benign and malignant ascites,and to explore the difference in COX-2 mRNA expression among different diseases.METHODS:A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups:benign ascites(n=21)and malignant ascites(n=15).Benign ascites included cirrhotic ascites(n=10)and tuberculous ascites(n=5).Malignant ascites included oophoroma(n=7),cancer of colon(n=5),cancer of the liver(n=6),gastric cancer(n=2),and bladder carcinoma(n=1).The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction(RT-PCR)technology,and the positive rate of COX-2 mRNA was compared between different diseases.RESULTS:The positive rate of COX-2 mRNA in malignant ascites was 42.9%(9/21),which was significantly higher than in benign ascites,6.7%(1/15),difference being significant between these two groups(χ2=4.051,P=0.044).The proportion of the positive rate in the malignant ascites was as follows:ovarian cancers57.1%(4/7),colon cancer 40.0%(2/5),liver cancer33.3%(2/6),gastric cancer 50.0%(1/2),and bladder cancer 0.00%(0/1).However,there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites(χ2=1.614,P=0.806).Among the benign ascites,COX-2 mRNA levels were different between the tuberculous ascites(0/5)and cirrhotic ascites(1/10),but there was no significant difference(P=1.000).CONCLUSION:COX-2 mRNA,detected by RT-PCR,is useful in the differential diagnosis of benign and malignant ascites,which also has potential value in the clinical diagnosis of tumors.
文摘【目的】通过检测腹水COX-2 m RNA的表达水平,探讨COX-2 m RNA对腹水鉴别诊断的价值和意义。【方法】收集2014年6月至2015年2月期间到我院就诊并明确诊断的恶性腹水34例,良性腹水15例。采用实时荧光定量PCR方法检测腹水中COX-2 m RNA的表达水平,比较COX-2 m RNA在良恶性腹水中表达的差异,及在不同原发疾病中的表达情况。【结果】34例恶性腹水及15例良性腹水均扩增出COX-2,良恶性腹水中COX-2 m RNA的相对表达水平与性别、年龄构成比无关系,差异无统计学意义(P>0.05)。恶性腹水和良性腹水COX-2 m RNA的表达水平分别为7.93(5.04,16.58)和0.95(0.57,1.79),即恶性腹水COX-2 m RNA的表达水平显著高于良性腹水,差异有统计学意义(P<0.05)。恶性腹水COX-2m RNA的表达水平在不同原发疾病中差异无统计学意义(P>0.05),良性腹水COX-2 m RNA表达水平在肝硬化和结核性腹膜炎中分别为1.55(0.55,2.55)和0.87(0.61,1.38),差异无统计学意义(P>0.05)。【结论】COX-2 m RNA在恶性腹水中的表达水平显著高于良性腹水,在鉴别诊断腹水性质中具有重要的参考价值。
