Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteris...Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C4 column （250 mm×4.6 mm×5μm） and Shiseido CAPCELL PAK SG 300 C18 column （250 mm× 4.6 mm×5 μm） were used in the experiment. Phase A was composed of 0.1% （V/V） trifluoroacetic acid （TFA） in ultrapure water and Phase B （organic phase） was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min-1. The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30℃. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%-104.38%, C18 column had stronger ability to resist acid and more stable, and the method with C4 column had excellent sensitivities and good separation.展开更多
A column chromatographic method has been developed for the separation and determination of cerium(Ⅲ) using poly[dibenzo-18-crown-6].The separation was carried out in L-valine medium.The adsorption of cerium(Ⅲ) was q...A column chromatographic method has been developed for the separation and determination of cerium(Ⅲ) using poly[dibenzo-18-crown-6].The separation was carried out in L-valine medium.The adsorption of cerium(Ⅲ) was quantitative from 1×10-1 to 1×10-4 mol/L L-valine.Amongst the various eluents,1.0-8.0 mol/L hydrochloric acid,1.0-8.0 mol/L hydrobromic acid,1.0-8.0 mol/L perchloric acid,1.0-2.0 mol/L sulfuric acid and 4.0-5.0 mol/L acetic acid,were found to be the efficient eluents for cerium(Ⅲ).The capacity of poly[dibenzo-18-crown-6] for cerium(Ⅲ) was(0.428±0.01) mmol/g.The method was applied to the separation of cerium(Ⅲ) from associated elements link uranium(Ⅵ) and thorium(Ⅳ).It was also applied for the determination of cerium(Ⅲ) in geological samples.The method is simple,rapid and selective with good reproducibility(approximately±2%).展开更多
基金Supported by the Project of Science & Technology Plans in Heilongjiang Province in the 11th Five-year Period (GB07B407)
文摘Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C4 column （250 mm×4.6 mm×5μm） and Shiseido CAPCELL PAK SG 300 C18 column （250 mm× 4.6 mm×5 μm） were used in the experiment. Phase A was composed of 0.1% （V/V） trifluoroacetic acid （TFA） in ultrapure water and Phase B （organic phase） was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min-1. The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30℃. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%-104.38%, C18 column had stronger ability to resist acid and more stable, and the method with C4 column had excellent sensitivities and good separation.
基金This work was supported by the University Grant Commission for awarding Junior Research Fellowship.
文摘A column chromatographic method has been developed for the separation and determination of cerium(Ⅲ) using poly[dibenzo-18-crown-6].The separation was carried out in L-valine medium.The adsorption of cerium(Ⅲ) was quantitative from 1×10-1 to 1×10-4 mol/L L-valine.Amongst the various eluents,1.0-8.0 mol/L hydrochloric acid,1.0-8.0 mol/L hydrobromic acid,1.0-8.0 mol/L perchloric acid,1.0-2.0 mol/L sulfuric acid and 4.0-5.0 mol/L acetic acid,were found to be the efficient eluents for cerium(Ⅲ).The capacity of poly[dibenzo-18-crown-6] for cerium(Ⅲ) was(0.428±0.01) mmol/g.The method was applied to the separation of cerium(Ⅲ) from associated elements link uranium(Ⅵ) and thorium(Ⅳ).It was also applied for the determination of cerium(Ⅲ) in geological samples.The method is simple,rapid and selective with good reproducibility(approximately±2%).
