Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kin...Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kinβγ mutant, the levels of reactive oxygen species were decreased which lead to compromised hydration of the mutant pollen on the stigma. In this study, we analyzed gene expression in kinβγ mutant pollen by RNA-seq and found the expression of inward shaker K^＋ channel SPIK was down-regulated in the kinβγ /pollen. Furthermore, we showed that the pollen hydration of the Arabidopsis spik mutant was defective on the wild-type stigma, althoughthe mutant pollen demonstrated normal hydration in vitro. Additionally, the defective hydration of spik mutant pollen could not be rescued by the wild-type pollen on the stigma, indicating that the spik mutation deprived the capability of pollen absorption on the stigma. Our results suggest that the Arabidopsis SnRK1 complex regulates SPIK expression, which functions in determining pollen hydration on the stigma.展开更多
SARS virus spike gene fra gment was expressed by Ecoli expr ession systemThe fragment enclose s major neutralization epitope of the virusThe expressed protein wa s purified and an ELISA method was set upBy...SARS virus spike gene fra gment was expressed by Ecoli expr ession systemThe fragment enclose s major neutralization epitope of the virusThe expressed protein wa s purified and an ELISA method was set upBy using the recombinant,tw elve patients’ sera were detected The recombinant SARS coronavirus s pike protein offers an efficient wa y for serological diagnosis and is useful for epidemiological survey and vaccin e展开更多
基金supported by the Major Research Plan from the Ministry of Science and Technology of China(2013CB945100)the National Natural Science Foundation of China(31270358)
文摘Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kinβγ mutant, the levels of reactive oxygen species were decreased which lead to compromised hydration of the mutant pollen on the stigma. In this study, we analyzed gene expression in kinβγ mutant pollen by RNA-seq and found the expression of inward shaker K^＋ channel SPIK was down-regulated in the kinβγ /pollen. Furthermore, we showed that the pollen hydration of the Arabidopsis spik mutant was defective on the wild-type stigma, althoughthe mutant pollen demonstrated normal hydration in vitro. Additionally, the defective hydration of spik mutant pollen could not be rescued by the wild-type pollen on the stigma, indicating that the spik mutation deprived the capability of pollen absorption on the stigma. Our results suggest that the Arabidopsis SnRK1 complex regulates SPIK expression, which functions in determining pollen hydration on the stigma.
文摘SARS virus spike gene fra gment was expressed by Ecoli expr ession systemThe fragment enclose s major neutralization epitope of the virusThe expressed protein wa s purified and an ELISA method was set upBy using the recombinant,tw elve patients’ sera were detected The recombinant SARS coronavirus s pike protein offers an efficient wa y for serological diagnosis and is useful for epidemiological survey and vaccin e
