Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or...Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.展开更多
为有效防控云南茶树重要的食叶性害虫茶谷蛾(Agriophara rhombata),筛选防治茶谷蛾的适用药及适用量,指导科学用药。在室内采用浸叶法测定24~96 h 4种不同稀释倍数杀虫剂对茶谷蛾3龄幼虫的毒力作用。结果表明,4种化学杀虫剂对3龄茶谷蛾...为有效防控云南茶树重要的食叶性害虫茶谷蛾(Agriophara rhombata),筛选防治茶谷蛾的适用药及适用量,指导科学用药。在室内采用浸叶法测定24~96 h 4种不同稀释倍数杀虫剂对茶谷蛾3龄幼虫的毒力作用。结果表明,4种化学杀虫剂对3龄茶谷蛾幼虫的毒力作用由强到弱表现为:24~72 h时,6%乙基多杀菌素悬浮剂(SC)>15%茚虫威乳油(EC)>15%唑虫酰胺SC>24%虫螨腈SC;96 h时,6%乙基多杀菌素SC>24%虫螨腈SC>15%茚虫威EC>15%唑虫酰胺SC。6%乙基多杀菌素SC与15%茚虫威EC具有较强毒杀力及速效性,可作为应急防治茶谷蛾的推荐药剂;24%虫螨腈SC发挥作用较缓,具有明显的时间—剂量—死亡率效应。研究结果可为云南茶区茶谷蛾的防治提供参考基础和依据。展开更多
明确乙基多杀菌素和烯啶虫胺等10种药剂对甘蔗蓟马的田间防治效果,为甘蔗蓟马防治提供科学指导。在甘蔗蓟马发生高峰期,采用背负式电动喷雾器将药剂喷施至甘蔗心叶及与心叶相连的顺数第1、2叶片,于药后3、10 d分别测定对蓟马的防治效果...明确乙基多杀菌素和烯啶虫胺等10种药剂对甘蔗蓟马的田间防治效果,为甘蔗蓟马防治提供科学指导。在甘蔗蓟马发生高峰期,采用背负式电动喷雾器将药剂喷施至甘蔗心叶及与心叶相连的顺数第1、2叶片,于药后3、10 d分别测定对蓟马的防治效果。结果表明:60 g/L乙基多杀菌素SC 36 g a.i./hm^(2)、50%烯啶虫胺WP 120 g a.i./hm^(2)对甘蔗蓟马的防效优于其他药剂,药后3 d的防效分别为93.3%、90.8%,药后10 d的防效分别为82.5%、84.6%,2种药剂无显著差异;其余药剂药后3、10 d防效均低于75%。建议在甘蔗蓟马发生高峰期,使用60 g/L乙基多杀菌素SC 36 g a.i./hm^(2)或50%烯啶虫胺WP 120 g a.i./hm^(2)能有效防控甘蔗蓟马。展开更多
基金funded by the National Natural Science Foundation of China(82002166)the fellowship of China Postdoctoral Science Foundation(2020M672676)+3 种基金the Guangdong Project of Basic and Applied Basic Research(20201910240000150)funded by the National Natural Science Foundation of China(81902093)Guangzhou Science and Technology Project(202002030100)Funding from State Key Laboratory of Biocontrol(Sun Yat-sen University)(2020SKLBC-KF01).
文摘Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.
文摘明确乙基多杀菌素和烯啶虫胺等10种药剂对甘蔗蓟马的田间防治效果,为甘蔗蓟马防治提供科学指导。在甘蔗蓟马发生高峰期,采用背负式电动喷雾器将药剂喷施至甘蔗心叶及与心叶相连的顺数第1、2叶片,于药后3、10 d分别测定对蓟马的防治效果。结果表明:60 g/L乙基多杀菌素SC 36 g a.i./hm^(2)、50%烯啶虫胺WP 120 g a.i./hm^(2)对甘蔗蓟马的防效优于其他药剂,药后3 d的防效分别为93.3%、90.8%,药后10 d的防效分别为82.5%、84.6%,2种药剂无显著差异;其余药剂药后3、10 d防效均低于75%。建议在甘蔗蓟马发生高峰期,使用60 g/L乙基多杀菌素SC 36 g a.i./hm^(2)或50%烯啶虫胺WP 120 g a.i./hm^(2)能有效防控甘蔗蓟马。
