AIM: To study the interactions between human gastriccarcinoma cell (HGCC) and human vascular endothelialcell (HVEC), and if the expression of sphingosine kinase(SPK) gene was involved in these interactions.METHODS: Th...AIM: To study the interactions between human gastriccarcinoma cell (HGCC) and human vascular endothelialcell (HVEC), and if the expression of sphingosine kinase(SPK) gene was involved in these interactions.METHODS: The specific inhibitor to SPK, dimethylsphingosine (DMS), was added acting on HGCC andHVEC, then the cell proliferation was measured by MTT.The conditioned mediums (CMs) of HGCC and HVECwere prepared. The CM of one kind of cell was added tothe other kind of cell, and the cell proliferation wasmeasured by MTr. After the action of CM, the cellularexpression of SPK gene in mRNA level was detectedwith in situ hybridization(ISH).RESULTS: DMS could almost completely inhibit theproliferation of HGCC and HVEC. The growth inhibitoryrates could amount to 97.21%, 83.42 %, respectively(P＜0.01). The CM of HGCC could stimulate the growthof HVEC (2.70±0.0:1, P＜0.01) while the CM of HVEC couldinhibit the growth of HGCC (52.97±0.01 %, P＜0.01).There was no significant change in the mRNA level ofSPK gene in one kind of cell after the action of the CM ofthe other kind of cell.CONCLUSION: SPK plays a key role in regulating theproliferation of HGCC and HVEC. There exist complicatedinteractions between HGCC and HVEC. HGCC cansignificantly stimulate the growth of HVEC while HVECcan significantly inhibit the growth of HGCC. Theexpression of SPK gene is not involved in the interactions.展开更多
This paper introduced the aviation biofuel technologies, among which the Fischer-Tropsch （FT） syn- thesis and the renewable jet fuel process provide alternative fuels for the potential environmental benefit of re- d...This paper introduced the aviation biofuel technologies, among which the Fischer-Tropsch （FT） syn- thesis and the renewable jet fuel process provide alternative fuels for the potential environmental benefit of re- duced life cycle greenhouse gas （GHG） emissions and the economic benefits associated with increased fuel availability and lower fuel costs. Tianjin University developed a new method （C-LTM process ） to produce wide- cut aviation biofuel from fatty acid methyl esters. All renewable jet fuel processes are low capital cost processes for producing high quality synthetic paraffinic kerosene （SPK） that has superior product properties to other op- tions available today with higher cetane number, lower cloud point and lower emissions, and it can be used in today＇ s tanks, pipelines, pumps and automobiles without any changes, which will save significant expense for renewable grows.展开更多
文摘AIM: To study the interactions between human gastriccarcinoma cell (HGCC) and human vascular endothelialcell (HVEC), and if the expression of sphingosine kinase(SPK) gene was involved in these interactions.METHODS: The specific inhibitor to SPK, dimethylsphingosine (DMS), was added acting on HGCC andHVEC, then the cell proliferation was measured by MTT.The conditioned mediums (CMs) of HGCC and HVECwere prepared. The CM of one kind of cell was added tothe other kind of cell, and the cell proliferation wasmeasured by MTr. After the action of CM, the cellularexpression of SPK gene in mRNA level was detectedwith in situ hybridization(ISH).RESULTS: DMS could almost completely inhibit theproliferation of HGCC and HVEC. The growth inhibitoryrates could amount to 97.21%, 83.42 %, respectively(P＜0.01). The CM of HGCC could stimulate the growthof HVEC (2.70±0.0:1, P＜0.01) while the CM of HVEC couldinhibit the growth of HGCC (52.97±0.01 %, P＜0.01).There was no significant change in the mRNA level ofSPK gene in one kind of cell after the action of the CM ofthe other kind of cell.CONCLUSION: SPK plays a key role in regulating theproliferation of HGCC and HVEC. There exist complicatedinteractions between HGCC and HVEC. HGCC cansignificantly stimulate the growth of HVEC while HVECcan significantly inhibit the growth of HGCC. Theexpression of SPK gene is not involved in the interactions.
文摘This paper introduced the aviation biofuel technologies, among which the Fischer-Tropsch （FT） syn- thesis and the renewable jet fuel process provide alternative fuels for the potential environmental benefit of re- duced life cycle greenhouse gas （GHG） emissions and the economic benefits associated with increased fuel availability and lower fuel costs. Tianjin University developed a new method （C-LTM process ） to produce wide- cut aviation biofuel from fatty acid methyl esters. All renewable jet fuel processes are low capital cost processes for producing high quality synthetic paraffinic kerosene （SPK） that has superior product properties to other op- tions available today with higher cetane number, lower cloud point and lower emissions, and it can be used in today＇ s tanks, pipelines, pumps and automobiles without any changes, which will save significant expense for renewable grows.
