在广西大学校园内,对美人蕉(C anna ind ica L.)和花叶美人蕉(C anna g lauca L.)的叶绿素含量、净光合速率、蒸腾速率和水分利用效率等生理特性进行测定和对比分析。结果表明:2种植物的净光合速率和蒸腾速率日变化均为“双峰型”曲线,...在广西大学校园内,对美人蕉(C anna ind ica L.)和花叶美人蕉(C anna g lauca L.)的叶绿素含量、净光合速率、蒸腾速率和水分利用效率等生理特性进行测定和对比分析。结果表明:2种植物的净光合速率和蒸腾速率日变化均为“双峰型”曲线,1天中的最高峰出现在10:00左右,16:00左右出现第2个较小的峰。美人蕉的各项生理指标均高于花叶美人蕉,其中叶绿素含量和日平均净光合速率分别是花叶美人蕉的2.67倍和1.78倍,而且,美人蕉具有较高的水分利用效率。两者的蒸腾速率日平均值差异不大。展开更多
Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic pla...Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic plants,codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay(ELISA) examination,immunogold localization,and 1 H-nuclear magnetic resonance( 1 H-NMR) . Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions.The plants of transgenic line 1(L1) showed significantly higher net photosynthetic rate(Pn) and Pn recovery rate under high(45°C,4 h) and low temperature(1°C,48 h) treatments,and higher photosynthetic rate under high salinity conditions(100,200,and 300 mmol/L NaCl,respectively) than the wild-type plants.The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine,which is not found in the wild-type plants.Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L.spp.chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.展开更多
文摘在广西大学校园内,对美人蕉(C anna ind ica L.)和花叶美人蕉(C anna g lauca L.)的叶绿素含量、净光合速率、蒸腾速率和水分利用效率等生理特性进行测定和对比分析。结果表明:2种植物的净光合速率和蒸腾速率日变化均为“双峰型”曲线,1天中的最高峰出现在10:00左右,16:00左右出现第2个较小的峰。美人蕉的各项生理指标均高于花叶美人蕉,其中叶绿素含量和日平均净光合速率分别是花叶美人蕉的2.67倍和1.78倍,而且,美人蕉具有较高的水分利用效率。两者的蒸腾速率日平均值差异不大。
基金Project supported by the National Science Foundation of China (No.30571146)the National Key Basic Research Special Foundation of China(No.G1999011700)
文摘Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic plants,codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay(ELISA) examination,immunogold localization,and 1 H-nuclear magnetic resonance( 1 H-NMR) . Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions.The plants of transgenic line 1(L1) showed significantly higher net photosynthetic rate(Pn) and Pn recovery rate under high(45°C,4 h) and low temperature(1°C,48 h) treatments,and higher photosynthetic rate under high salinity conditions(100,200,and 300 mmol/L NaCl,respectively) than the wild-type plants.The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine,which is not found in the wild-type plants.Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L.spp.chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.
