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Design of Cold-Junction Compensation and Disconnection Detection Circuits of Various Thermocouples and Implementation of Multi-Channel Interfaces Using Them-A Secondary Publication
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作者 Hyeong-Woo Cha 《Journal of Electronic Research and Application》 2024年第1期93-105,共13页
Cold-junction compensation(CJC)and disconnection detection circuit design of various thermocouples(TC)and multi-channel TC interface circuits were designed.The CJC and disconnection detection circuit consists of a CJC... Cold-junction compensation(CJC)and disconnection detection circuit design of various thermocouples(TC)and multi-channel TC interface circuits were designed.The CJC and disconnection detection circuit consists of a CJC semiconductor device,an instrumentation amplifier(IA),two resistors,and a diode for disconnection detection.Based on the basic circuit,a multi-channel interface circuit was also implemented.The CJC was implemented using compensation semiconductor and IA,and disconnection detection was detected by using two resistors and a diode so that IA input voltage became-0.42 V.As a result of the experiment using R-type TC,the error of the designed circuit was reduced from 0.14 mV to 3μV after CJC in the temperature range of 0°C to 1400°C.In addition,it was confirmed that the output voltage of IA was saturated from 88 mV to-14.2 V when TC was disconnected from normal.The output voltage of the designed circuit was 0 V to 10 V in the temperature range of 0°C to 1400°C.The results of the 4-channel interface experiment using R-type TC were almost identical to the CJC and disconnection detection results for each channel.The implemented multi-channel interface has a feature that can be applied equally to E,J,K,T,R,and S-type TCs by changing the terminals of CJC semiconductor devices and adjusting the IA gain. 展开更多
关键词 R-type thermocouple(TC) Cold-junction compensation(CJC) TC disconnection detection multi-channel interface circuit Sensor interface
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Parametric study on contact sensors for MASW measurement-based interfacial debonding detection for SCCS
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作者 Chen Hongbing Pang Xin +3 位作者 Gan Shiyu Li Yuanyuan Gokarna Chalise Nie Xin 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第2期331-344,共14页
Steel-concrete composite structures(SCCS)have been widely used as primary load-bearing components in large-scale civil infrastructures.As the basis of the co-working ability of steel plate and concrete,the bonding sta... Steel-concrete composite structures(SCCS)have been widely used as primary load-bearing components in large-scale civil infrastructures.As the basis of the co-working ability of steel plate and concrete,the bonding status plays an essential role in guaranteeing the structural performance of SCCS.Accordingly,efficient non-destructive testing(NDT)on interfacial debondings in SCCS has become a prominent research area.Multi-channel analysis of surface waves(MASW)has been validated as an effective NDT technique for interfacial debonding detection for SCCS.However,the feasibility of MASW must be validated using experimental measurements.This study establishes a high-frequency data synchronous acquisition system with 32 channels to perform comparative verification experiments in depth.First,the current sensing approaches for high-frequency vibration and stress waves are summarized.Secondly,three types of contact sensors,namely,piezoelectric lead-zirconate-titanate(PZT)patches,accelerometers,and ultrasonic transducers,are selected for MASW measurement.Then,the selection and optimization of the force hammer head are performed.Comparative experiments are carried out for the optimal selection of ultrasonic transducers,PZT patches,and accelerometers for MASW measurement.In addition,the influence of different pasting methods on the output signal of the sensor array is discussed.Experimental results indicate that optimized PZT patches,acceleration sensors,and ultrasonic transducers can provide efficient data acquisition for MASW-based non-destructive experiments.The research findings in this study lay a solid foundation for analyzing the recognition accuracy of contact MASW measurement using different sensor arrays. 展开更多
关键词 steel-concrete composite structures(SCCS) interface debonding detection multi-channel analysis of surface waves(MASW) sensor selection comparative experimental study
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A New Dual-electrode and Multi-channel Electrochemical Detection System for Capillary Electrophoresis
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作者 BingYiYANG JinYuanMO RongLAI 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第4期439-442,共4页
A new type of dual-electrode and multi-channel electrochemical detection technology for capillary electrophoresis is described in this paper. Two detectors(the amperometric detector and the conductometric detector) or... A new type of dual-electrode and multi-channel electrochemical detection technology for capillary electrophoresis is described in this paper. Two detectors(the amperometric detector and the conductometric detector) or two conductometric detectors are connected to the same capillary electrophoresis system. The whole system possesses the advantages of the two electrochemical detectors including sparing time, improving the analytical speed and expanding the sample range. The working electrode and detector cell are handled easily. The system was applied to sample detection with satisfactory results. 展开更多
关键词 Capillary electrophoresis DUAL-ELECTRODE multi-channel electrochemical detection.
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Research of multi-channel impulse noise detection algorithms based on template
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作者 赵薇 Xie Qianli +1 位作者 Wu Yin Zhang Qin 《High Technology Letters》 EI CAS 2015年第2期147-154,共8页
Attenuating the undesired audio noise generated by impulse noise,such as shot and scream of brakes,is specially useful for real-time audio recording of TV or broadcasting live report.On the basis of impulse noise dete... Attenuating the undesired audio noise generated by impulse noise,such as shot and scream of brakes,is specially useful for real-time audio recording of TV or broadcasting live report.On the basis of impulse noise detection algorithms based on template,this paper improves the method of establishing the template by using multiple microphones to pick up noise corrupted signals and impulse noises in the environment.The universal of thresholds is found and a detection algorithm with slope as the characteristic is proposed by comparing a variety of feature extraction algorithms.The proposed algorithm gets a significant improvement in testing speed and accuracy,which means it is suitable for real-time processing of audio signals. 展开更多
关键词 impulse noise detection TEMPLATE multi-channel universal of thresholds slop algorithm (SA)
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A Multi-Channel Fusion Based Newborn Seizure Detection
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作者 Malarvili BalaKrishnan Paul Colditz Boualeum Boashash 《Journal of Biomedical Science and Engineering》 2014年第8期533-545,共13页
We propose and compare two multi-channel fusion schemes to utilize the information extracted from simultaneously recorded multiple newborn electroencephalogram (EEG) channels for seizure detection. The first approach ... We propose and compare two multi-channel fusion schemes to utilize the information extracted from simultaneously recorded multiple newborn electroencephalogram (EEG) channels for seizure detection. The first approach is known as the multi-channel feature fusion. It involves concatenating EEG feature vectors independently obtained from the different EEG channels to form a single feature vector. The second approach, called the multi-channel decision/classifier fusion, is achieved by combining the independent decisions of the different EEG channels to form an overall decision as to the existence of a newborn EEG seizure. The first approach suffers from the large dimensionality problem. In order to overcome this problem, three different dimensionality reduction techniques based on the sum, Fisher’s linear discriminant and symmetrical uncertainty (SU) were considered. It was found that feature fusion based on SU technique outperformed the other two techniques. It was also shown that feature fusion, which was developed on the basis that there was inter-dependence between recorded EEG channels, was superior to the independent decision fusion. 展开更多
关键词 EEG NEWBORN SEIZURE detection multi-channel Feature FUSION Decision/Classifier FUSION
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The Detection of SARS-CoV with SPR Biosensor
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作者 Yujie Wang Dafu Cui +5 位作者 Junbo Wang Haoyuan Cai Zipan Zheng Wei Deng Chuan Qin Xiuhong Yang 《稀有金属材料与工程》 SCIE EI CAS CSCD 北大核心 2006年第A03期288-290,共3页
SPR (Surface Plasmon Resonance) is an emerging optical biosensor which can monitor the processes at metal interface in real time without labeling requirements.Instrument SPR-2004 can sense two areas in one channel and... SPR (Surface Plasmon Resonance) is an emerging optical biosensor which can monitor the processes at metal interface in real time without labeling requirements.Instrument SPR-2004 can sense two areas in one channel and get two signals at same time in one test.One signal is the graph of the reaction;the other is the graph of reference.SPR biochip is modified with dextran,and only reaction area can be used to immobilize the protein.Two methods of antibody immobilization on chip were tested.One was to immobilize directly,the other was to immobilize protein A firstly and then the antibody was caught by immobilized protein A.The latter was chosen as suitable for retention of the native binding ability with virus of antibody.The latter chip was used to detect SARS-Cov.The signal of detection reached to 60 units within 40 min.It was 55 units even after reference. 展开更多
关键词 spr-2004 BIOSENSOR SARS-COV detection ANTIBODY
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Advances in Phase Detection Technique of Surface Plasmon Resonance Biosensor 被引量:1
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作者 Yu Xinglong Wei Xing Deng Yan Liu Fangfang 《稀有金属材料与工程》 SCIE EI CAS CSCD 北大核心 2006年第A03期299-304,共6页
Surface plasmon resonance (SPR) sensing is an optical method based on evanescent wave.SPR biosensor can detect interaction of label-free biomolecules in real-time.With further development,it can become a research ins... Surface plasmon resonance (SPR) sensing is an optical method based on evanescent wave.SPR biosensor can detect interaction of label-free biomolecules in real-time.With further development,it can become a research instrument in proteomics.SPR biosensor can be divided intensity measurement and phase measurement,and the latter possesses higher sensitivity than the former one.This paper attempts to summarize the SPR phase detection theory,discuss the major developments,compare the merits and deficiencies of various methods,and look forward to future prospects. 展开更多
关键词 surface plasmon resonance(spr) phase detection ELLIPSOMETRY HETERODYNE INTERFEROMETRY
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A multi-channel chemical sensor and its application in detecting hydrothermal vents 被引量:1
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作者 Zhen Cai A J Mur Luis +3 位作者 Jiwan Han Kui Wang Huawei Qin Ying Ye 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2019年第9期128-134,共7页
There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-cha... There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-channel chemical sensor to detect such anomalies which can be used in deep-sea at depths of more than 4 000 m. The design allowed five all-solid-state electrodes to be mounted on it and each (apart from one reference electrode) could be changed according to chemicals to be measured. Two experiments were conducted using the chemical sensors. The first was a shallow-sea trial which included sample measurements and in situ monitoring. pH, Eh, CO3^2- and SO4^2- electrodes were utilized to demonstrate that the chemical sensor was accurate and stable outside the laboratory. In the second experiment, the chemical sensor was integrated with pH, Eh, CO3^2- and H2S electrodes, and was used in 29 scans of the seabed along the Southwest Indian Ridge (SWIR) to detect hydrothermal vents, from which 27 sets of valid data were obtained. Hydrothermal vents were identified by analyzing the chemical anomalies, the primary judging criteria were decreasing voltages of Eh and H2S, matched by increasing voltages of pH and CO3^2- . We proposed that simultaneous detection of changes in these parameters will indicate a hydrothermal vent. Amongst the 27 valid sets of data, five potential hydrothermal vents were targeted using the proposed method. We suggest that our sensors could be widely employed by marine scientists. 展开更多
关键词 CHEMICAL sensor multi-channel HYDROTHERMAL VENTS detection CHEMICAL ANOMALIES SWIR
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利用胶体金复合抗体提高SPR生物传感器的微生物检测精度 被引量:12
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作者 殷涌光 葛晶 +1 位作者 于庆宇 王凯 《吉林大学学报(工学版)》 EI CAS CSCD 北大核心 2005年第4期446-450,共5页
使用集成化手持式SpreetaTMSPR传感器快速检测大肠杆菌,将亲和素生物素系统用于SPR免疫传感器以保证检测的准确性。利用大肠杆菌抗体的免疫吸附反应,采用一次抗体抗原二次抗体的夹心式方法增加质量,扩大检测大肠杆菌的信号。引入胶体金... 使用集成化手持式SpreetaTMSPR传感器快速检测大肠杆菌,将亲和素生物素系统用于SPR免疫传感器以保证检测的准确性。利用大肠杆菌抗体的免疫吸附反应,采用一次抗体抗原二次抗体的夹心式方法增加质量,扩大检测大肠杆菌的信号。引入胶体金复合抗体作为二次抗体大幅度增加质量,进一步扩大了检测信号。同时延长胶体金复合抗体与微生物的结合过程,使检测信号进一步稳定与放大,从而显著提高了检测精度,使该传感器对大肠杆菌的检测精度由106cfu/mL提高到101cfu/mL。 展开更多
关键词 仪器检验 胶体金复合抗体 spr生物传感器 微生物 检测精度
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基于高斯拟合的光纤型SPR信号的峰值检测算法 被引量:9
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作者 周鹏 张文斌 +4 位作者 王军星 孙翠迎 刘瑾 苏荣欣 王学民 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2016年第6期1949-1953,共5页
光纤型表面等离子共振光谱具有半峰宽较宽,共振峰不尖锐等特征,传统的针对棱镜型SPR光谱的峰值检测算法无法准确定位此类光谱的共振峰值。为了准确计算光纤型SPR光谱的共振波长,提出了一种基于信赖域算法的高斯拟合方法,在拟合的光谱曲... 光纤型表面等离子共振光谱具有半峰宽较宽,共振峰不尖锐等特征,传统的针对棱镜型SPR光谱的峰值检测算法无法准确定位此类光谱的共振峰值。为了准确计算光纤型SPR光谱的共振波长,提出了一种基于信赖域算法的高斯拟合方法,在拟合的光谱曲线上利用一维搜索的方式确定峰值位置。通过对标准甘油光谱数据的处理,证明高斯拟合法能够适应光纤型SPR光谱的特征,波长计算准确。通过搭建的SPR系统,实验测量了不同浓度下蔗糖溶液的SPR光谱数据,并分别采用加权质心法,追踪质心法和该研究提出的高斯拟合法进行共振波长的计算。结果表明高斯拟合法能够有效提升分辨率,且运算速度较快,有利于工程集成。 展开更多
关键词 spr光谱 高斯拟合法 信赖域算法 峰值检测
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利用参比型SPR生物传感器进行SARS病毒抗原检测的研究 被引量:4
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作者 王于杰 崔大付 +5 位作者 邓巍 秦川 王军波 蔡浩原 关昕 王利 《传感技术学报》 CAS CSCD 北大核心 2007年第10期2151-2154,共4页
参比型SPR生物传感器是一种可以在单通道内实现对检测、参比两个位点同时检测的新型SPR分析系统.利用参比型SPR生物传感器在线制备了两种SARS病毒抗原的检测芯片.直接固定抗体法制备的检测芯片在检测时基本无反应;蛋白A固定抗体法制备... 参比型SPR生物传感器是一种可以在单通道内实现对检测、参比两个位点同时检测的新型SPR分析系统.利用参比型SPR生物传感器在线制备了两种SARS病毒抗原的检测芯片.直接固定抗体法制备的检测芯片在检测时基本无反应;蛋白A固定抗体法制备的芯片在通入灭活SARS病毒时检测信号明显,参比信号略有上升.第二种芯片可以直接检测到病毒培养液上清中的灭活SARS病毒,检测灵敏度已达到1.66×104PFU/mL.参比位点可以用来检测待测物中杂质引起的非特异性吸附. 展开更多
关键词 生物传感器 SARS病毒 检测 参比 spr
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基于生物素-亲和素放大的SPR传感器检测大肠杆菌研究 被引量:10
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作者 刘儒平 王程 +3 位作者 徐万帮 岳钊 牛文成 刘国华 《传感技术学报》 CAS CSCD 北大核心 2013年第6期757-761,共5页
建立了一种基于表面等离子体共振(SPR)技术的大肠杆菌特异性快速检测方法。采用EDC/NHS将葡聚糖修饰的CM5芯片表面活化,通过亲和素固定生物素标记的二抗(羊抗兔IgG抗体),利用一抗(兔抗大肠杆菌ATCC25922单克隆抗体)和二抗反应,将兔抗大... 建立了一种基于表面等离子体共振(SPR)技术的大肠杆菌特异性快速检测方法。采用EDC/NHS将葡聚糖修饰的CM5芯片表面活化,通过亲和素固定生物素标记的二抗(羊抗兔IgG抗体),利用一抗(兔抗大肠杆菌ATCC25922单克隆抗体)和二抗反应,将兔抗大肠杆菌单克隆抗体固定在传感芯片表面。利用一抗和二抗的质量扩增效应和生物素-亲和素的多级放大效应,实现了对低浓度大肠杆菌ATCC25922的快速检测,提高了SPR传感器灵敏度。利用NaOH溶液对芯片再生,可对多个不同浓度样品进行检测,采用相对响应单位(RU)记录数据。本传感芯片对大肠杆菌ATCC25922响应的线性范围为1.5×102 CFU/mL~1.5×107 CFU/mL,检测限为1.5×102 CFU/mL,相关系数r为0.981 5。这种方法简便快捷,有望成为一种在线检测治病菌的有力手段。 展开更多
关键词 spr传感器 生物素-亲和素多级放大 大肠杆菌ATCC25922 快速检测
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使用SPR生物传感器快速检测大肠杆菌E.Coli 0157∶H7 被引量:19
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作者 葛晶 殷涌光 王凯 《吉林大学学报(工学版)》 EI CAS CSCD 北大核心 2005年第2期214-218,共5页
利用大肠杆菌抗体的免疫吸附反应,使用集成化手持式 SpreetaTM SPR传感器快速检测大肠杆菌E.Coli 0157∶H7,采用亲和素 生物素系统放大检测的响应信号,并引入复合抗体作为二次抗体,使该传感器对大肠杆菌的检测限由106cfu/mL下降到105cfu... 利用大肠杆菌抗体的免疫吸附反应,使用集成化手持式 SpreetaTM SPR传感器快速检测大肠杆菌E.Coli 0157∶H7,采用亲和素 生物素系统放大检测的响应信号,并引入复合抗体作为二次抗体,使该传感器对大肠杆菌的检测限由106cfu/mL下降到105cfu/mL。 展开更多
关键词 食品检测 spr生物传感器 快速检测 大肠杆菌 复合抗体
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纳米金标记抗体增强SPR检测大肠杆菌O157∶H7 被引量:8
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作者 刘霞 李蓉卓 +2 位作者 李蕾 李文进 周春娇 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2013年第6期1333-1338,共6页
将金纳米粒子(AuNPs)标记的大肠杆菌O157∶H7(E.coli O157∶H7)的多克隆抗体(PAb)作为二抗,采用氨基偶联法将PAb固定在传感器表面作为一抗,通过三明治方法用双通道表面等离子体子共振(SPR)传感器对E.coli O157∶H7进行检测,并与SPR直接... 将金纳米粒子(AuNPs)标记的大肠杆菌O157∶H7(E.coli O157∶H7)的多克隆抗体(PAb)作为二抗,采用氨基偶联法将PAb固定在传感器表面作为一抗,通过三明治方法用双通道表面等离子体子共振(SPR)传感器对E.coli O157∶H7进行检测,并与SPR直接法检测进行了比较.结果表明,直接法的检出限为103cfu/mL,线性范围为103~109cfu/mL;AuNPs增强三明治法的检出限为10 cfu/mL,线性范围为10~1010cfu/mL,灵敏度比直接法提高了100倍,且具有更宽的检测范围.本方法不仅检测时间短,而且具有良好的选择性和重现性. 展开更多
关键词 金纳米粒子 大肠杆菌O157∶H7 表面等离子体子共振 标记 检测
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蛋白质微阵列SPR实时相位检测 被引量:2
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作者 余兴龙 魏星 +3 位作者 王鼎新 定翔 廖玮 赵新生 《物理化学学报》 SCIE CAS CSCD 北大核心 2005年第8期888-892,共5页
将表面等离子体共振(surfaceplasmonresonance,SPR)与空间相位调制检测结合,用一束准直的平行光照射传感芯片,在反射光路中引入渥拉斯顿棱镜,使光束中的p光和s光产生偏振光干涉形成条纹,生化反应的有关信息从干涉条纹的相位变化中得出.... 将表面等离子体共振(surfaceplasmonresonance,SPR)与空间相位调制检测结合,用一束准直的平行光照射传感芯片,在反射光路中引入渥拉斯顿棱镜,使光束中的p光和s光产生偏振光干涉形成条纹,生化反应的有关信息从干涉条纹的相位变化中得出.选择金膜厚度为30nm和40nm的两种芯片,分别用浓度为50%和20%的酒精-水溶液进行实验,得到SPR阵列图谱;选择金膜厚度为30nm,兔IgG作为阳性对照,牛血清白蛋白(BSA)作为阴性对照,与0.2mL的羊抗兔IgG、1.8mL水的混合溶液反应,得到阵列反应图谱.实验结果表明,这种方法具有抗干扰能力强,灵敏度高,无需标记和可实时检测等优点,能满足蛋白质芯片检测的要求. 展开更多
关键词 蛋白质微阵列 spr 相位检测 偏振光干涉
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SPR生物传感器快速检测醋酸甲羟孕酮的初步应用研究 被引量:5
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作者 黄强力 王晶钰 +3 位作者 韩雪清 林祥梅 王慧煜 梅琳 《中国畜牧兽医》 CAS 北大核心 2010年第3期102-105,共4页
动物源性食品中残留的醋酸甲羟孕酮(medroxy progesterone acetate,MPA)对人类健康有潜在的危害。利用SPR生物传感器快速检测MPA,对抗原的固定条件、抗体的浓度及芯片再生条件进行了优化,同时对抗体的稳定性及特异性进行了分析。结果显... 动物源性食品中残留的醋酸甲羟孕酮(medroxy progesterone acetate,MPA)对人类健康有潜在的危害。利用SPR生物传感器快速检测MPA,对抗原的固定条件、抗体的浓度及芯片再生条件进行了优化,同时对抗体的稳定性及特异性进行了分析。结果显示,该方法检测的最低限约为1 ng/mL。利用模拟标本进行平行测试表明,其与进口ELISA检测试剂盒结果基本一致。SPR生物传感器检测MPA,单个样品的检测时间小于5 min,非常适合现场的快速检测,具有广泛的应用前景。 展开更多
关键词 醋酸甲羟孕酮 spr生物传感器 快速检测
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表面等离子共振(SPR)——一种新型化学检测方法的原理 被引量:8
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作者 吴世康 汪鹏飞 《影像科学与光化学》 EI CAS CSCD 2008年第2期157-168,共12页
近年来,表面等离子共振(SPR)现象、由于在金属纳米材料研究以及在作为灵敏检测不同化学和生化物种信号的报告器方面有着特殊的应用价值,因而受到广泛的关注.本文较详细的介绍了表面等离子共振现象的形成机制,特别对如何利用本方法作为... 近年来,表面等离子共振(SPR)现象、由于在金属纳米材料研究以及在作为灵敏检测不同化学和生化物种信号的报告器方面有着特殊的应用价值,因而受到广泛的关注.本文较详细的介绍了表面等离子共振现象的形成机制,特别对如何利用本方法作为一种新型的物种检测报告器的有关问题做了较系统的讨论. 展开更多
关键词 表面等离子共振 金属纳米材料 化学物种检测 倏逝波
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应用SPR生物传感器检测酸菜发酵液中大肠杆菌 被引量:8
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作者 张文浩 郑先哲 初国超 《东北农业大学学报》 CAS CSCD 北大核心 2010年第6期117-121,共5页
应用SPR传感器可以快速检测酸菜发酵液中的大肠杆菌,以便检测和控制酸菜发酵液中的亚硝酸盐含量,保证发酵食品安全。采用生物素-亲和素系统增大SPR传感器折射信号,获得了传感器的折射率与大肠杆菌浓度的回归方程,实现了对酸菜发酵液中... 应用SPR传感器可以快速检测酸菜发酵液中的大肠杆菌,以便检测和控制酸菜发酵液中的亚硝酸盐含量,保证发酵食品安全。采用生物素-亲和素系统增大SPR传感器折射信号,获得了传感器的折射率与大肠杆菌浓度的回归方程,实现了对酸菜发酵液中大肠杆菌浓度快速,精确检测,提高大肠杆菌检测限到104cfu·mL-1。 展开更多
关键词 等离子共振生物传感器 酸菜发酵液 大肠杆菌 生物素-亲和素系统 检测限
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分子印迹膜SPR传感器检测氯磺隆的方法 被引量:2
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作者 张璐璐 崔大付 +3 位作者 蔡浩原 李辉 孙建海 陈兴 《微纳电子技术》 CAS 北大核心 2011年第4期254-257,共4页
研究使用了中国科学院电子学研究所自行研制的高灵敏度单通道SPR分析仪和进样装置,仪器为棱镜耦合型SPR传感器结构,其检测角度范围是40°~70°,折射率检测范围为1.04~1.47,谐振角的精度在0.001°。适用于SPR分析仪的分子... 研究使用了中国科学院电子学研究所自行研制的高灵敏度单通道SPR分析仪和进样装置,仪器为棱镜耦合型SPR传感器结构,其检测角度范围是40°~70°,折射率检测范围为1.04~1.47,谐振角的精度在0.001°。适用于SPR分析仪的分子印迹芯片采用PVC-MIP共聚膜法制备,SPR角度扫描结果表明,200nm以下厚度芯片具有较好的SPR吸收特性。以氯磺隆为模板的分子印迹膜与不加氯磺隆的分子印迹膜对比实验发现前者具有特异性结合的能力。实验对浓度0.1,0.2,0.5和1μg/mL的氯磺隆进行了SPR定点检测,这四种浓度氯磺隆的折射率响应信号满足线性关系,相关系数R=0.996 4。实验中对低浓度的氯磺隆进行反复检测,检测到50ng/mL的氯磺隆,满足农残检测的要求。 展开更多
关键词 传感器 表面等离子共振(spr) 分子印迹膜(MIP) 氯磺隆(CS) 连续检测
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基于SPR相位检测的蛋白质芯片信号获取与处理研究 被引量:3
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作者 余兴龙 刘俊锋 定翔 《生命科学仪器》 2005年第3期20-24,共5页
表面等离子体共振传感器有可能发展成为一种灵敏的高通量检测的蛋白质组学研究工具,而信号获取和数据处理是其关键之一。本文利用数值模拟全面研究了微阵列相位检测的分辨率与空间采样频率、采样周期数以及ADC位数等关系,详细分析了相... 表面等离子体共振传感器有可能发展成为一种灵敏的高通量检测的蛋白质组学研究工具,而信号获取和数据处理是其关键之一。本文利用数值模拟全面研究了微阵列相位检测的分辨率与空间采样频率、采样周期数以及ADC位数等关系,详细分析了相关、正弦拟合和傅立叶变换(FTP)等算法对相位检测的精度和误差等影响。研究结果表明,在理想状况下,4个CCD像素就能满足分辨率对光电转换的要求,而在有噪音的情况下,分辨率随着空间采样频率的增加而提高;信号周期数对分辨率影响不大,但有利于克服噪声影响,提高精度;ADC对分辨率影响最大,高分辨率需要选择多位ADC,8位ADC时分辨率约为0.15°,10位时约为0.05°,而12位时约为0.01°。 展开更多
关键词 spr 相位检测 蛋白质芯片 信号获取
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