目的探讨Sprouty2蛋白与人乳腺癌MCF-7细胞增殖与迁徙的关系。方法通过siRNA技术干扰MCF-7细胞Sprouty2基因的表达,通过Real time PCR、Western blotting以及细胞免疫荧光检测Sprouty2基因的干扰效果,划痕实验以及Transwell实验观察细...目的探讨Sprouty2蛋白与人乳腺癌MCF-7细胞增殖与迁徙的关系。方法通过siRNA技术干扰MCF-7细胞Sprouty2基因的表达,通过Real time PCR、Western blotting以及细胞免疫荧光检测Sprouty2基因的干扰效果,划痕实验以及Transwell实验观察细胞迁移和侵袭的能力,Western blotting检测MMP-2、MMP-9和MMP-13的表达。结果通过siRNA干扰,有效地干扰了Sprouty2基因的表达,建立了Sprouty2基因低表达的细胞株;Sprouty2基因沉默后,细胞的迁移和侵袭能力明显增强;沉默Sprouty2基因的MCF-7细胞中MMP-2、MMP-9和MMP-13蛋白较对照组均升高。结论 Sprouty2基因下调后,MCF-7细胞的迁移和侵袭能力明显提高,可能与MMP-2、MMP-9和MMP-13蛋白有关。展开更多
目的分析寻常型银屑病皮损组织中chemerin和Sprouty1蛋白表达与病情严重程度的相关性。方法选取2018年2月-2020年11月在无锡市第五人民医院和江南大学附属医院皮肤性病科门诊接受治疗的68例寻常型银屑病患者作为实验组,以同期在两家医...目的分析寻常型银屑病皮损组织中chemerin和Sprouty1蛋白表达与病情严重程度的相关性。方法选取2018年2月-2020年11月在无锡市第五人民医院和江南大学附属医院皮肤性病科门诊接受治疗的68例寻常型银屑病患者作为实验组,以同期在两家医院皮肤性病科门诊体检的68名健康者作为对照组。采集寻常型银屑病患者躯干或四肢的皮损组织及健康者的正常皮肤组织,采用免疫组化及Western blotting检测皮肤组织中chemerin、Sprouty1蛋白相对表达量,RT-PCR检测皮肤组织中chemerin、Sprouty1 mRNA相对表达量,采用Pearson相关分析法分析chemerin、Sprouty1蛋白相对表达量与银屑病皮损面积和严重程度指数(PASI)的相关性。结果免疫组化检测结果显示,两组皮肤组织中均有chemerin、Sprouty1蛋白的表达。Chemerin蛋白在实验组皮损脂肪组织中呈高表达,在对照组皮下脂肪组织中少量表达;Sprouty1蛋白在实验组皮损颗粒层细胞膜中少量表达,在对照组表皮全层细胞的细胞膜和细胞质中呈高表达。实验组chemerin mRNA(1.478±0.14 vs. 0.858±0.08,P<0.001)和蛋白(1.138±0.12 vs. 0.789±0.06,P<0.001)相对表达量明显高于对照组,Sprouty1 mRNA(0.837±0.04 vs. 1.346±0.09,P<0.001)和蛋白(0.646±0.05 vs. 1.212±0.12,P<0.001)相对表达量明显低于对照组。Pearson相关分析显示,chemerin蛋白相对表达量与PASI评分呈正相关(r=0.536,P<0.05),Sprouty1蛋白相对表达量与PASI评分呈负相关(r=-0.619,P<0.05)。结论寻常型银屑病皮损组织中chemerin及Sprouty1蛋白相对表达量与PASI评分存在相关性。展开更多
BACKGROUND:In vitro experiments and mice models have confirmed the importance of Sprouty 2(Spry2) in inhibiting tumorigenesis and the progression of human cancer.However,the prognostic value of Spry2 in cancer patient...BACKGROUND:In vitro experiments and mice models have confirmed the importance of Sprouty 2(Spry2) in inhibiting tumorigenesis and the progression of human cancer.However,the prognostic value of Spry2 in cancer patients remains unknown.This study is aimed to investigate the clinical relevance and prognostic significance of Spry2 expression in patients with hepatocellular carcinoma(HCC).METHODS:With samples from 240 randomly-selected HCC patients who underwent surgery,immunohistochemistry was used to investigate Spry2 expression on tissue microarrays.The correlation of Spry2 expression with survival was estimated by the Kaplan-Meier method and univariate/multivariate Cox proportional hazard regression analysis.Spry2,ERK and phospho-ERK expression in HCC cell lines was detected by Western blotting.RESULTS:Among the patients,86.3%(207 of 240) exhibited down-regulation of Spry2 expression.Patients negative for Spry2 showed poorer survival(P=0.002) and increased recurrence(P=0.003).Multivariate analysis further established Spry2 as an independent predictor of postoperative recurrence in HCC patients(HR=1.47;95% CI,1.02-2.08;P=0.037).Downregulation of Spry2 was associated with highly malignant phenotypes like vascular invasion and advanced tumor stages,and was positively correlated with the metastatic potential of HCC cell lines.CONCLUSION:In the era of molecular targeted therapy,the expression of Spry2 in HCC may have relevant clinical significance and turn out to be a key factor in prognostic assessment and in treatment planning.展开更多
文摘目的分析寻常型银屑病皮损组织中chemerin和Sprouty1蛋白表达与病情严重程度的相关性。方法选取2018年2月-2020年11月在无锡市第五人民医院和江南大学附属医院皮肤性病科门诊接受治疗的68例寻常型银屑病患者作为实验组,以同期在两家医院皮肤性病科门诊体检的68名健康者作为对照组。采集寻常型银屑病患者躯干或四肢的皮损组织及健康者的正常皮肤组织,采用免疫组化及Western blotting检测皮肤组织中chemerin、Sprouty1蛋白相对表达量,RT-PCR检测皮肤组织中chemerin、Sprouty1 mRNA相对表达量,采用Pearson相关分析法分析chemerin、Sprouty1蛋白相对表达量与银屑病皮损面积和严重程度指数(PASI)的相关性。结果免疫组化检测结果显示,两组皮肤组织中均有chemerin、Sprouty1蛋白的表达。Chemerin蛋白在实验组皮损脂肪组织中呈高表达,在对照组皮下脂肪组织中少量表达;Sprouty1蛋白在实验组皮损颗粒层细胞膜中少量表达,在对照组表皮全层细胞的细胞膜和细胞质中呈高表达。实验组chemerin mRNA(1.478±0.14 vs. 0.858±0.08,P<0.001)和蛋白(1.138±0.12 vs. 0.789±0.06,P<0.001)相对表达量明显高于对照组,Sprouty1 mRNA(0.837±0.04 vs. 1.346±0.09,P<0.001)和蛋白(0.646±0.05 vs. 1.212±0.12,P<0.001)相对表达量明显低于对照组。Pearson相关分析显示,chemerin蛋白相对表达量与PASI评分呈正相关(r=0.536,P<0.05),Sprouty1蛋白相对表达量与PASI评分呈负相关(r=-0.619,P<0.05)。结论寻常型银屑病皮损组织中chemerin及Sprouty1蛋白相对表达量与PASI评分存在相关性。
基金supported by grants from the National Key Sci-Tech Special Project of China(2008ZX10002-018/019)the Shanghai Rising-Star Program(10QA1401300)+1 种基金the National Natural Science Foundation of China(30901432)the Research Fund for the Doctoral Program of Higher Education of China (20090071120026)
文摘BACKGROUND:In vitro experiments and mice models have confirmed the importance of Sprouty 2(Spry2) in inhibiting tumorigenesis and the progression of human cancer.However,the prognostic value of Spry2 in cancer patients remains unknown.This study is aimed to investigate the clinical relevance and prognostic significance of Spry2 expression in patients with hepatocellular carcinoma(HCC).METHODS:With samples from 240 randomly-selected HCC patients who underwent surgery,immunohistochemistry was used to investigate Spry2 expression on tissue microarrays.The correlation of Spry2 expression with survival was estimated by the Kaplan-Meier method and univariate/multivariate Cox proportional hazard regression analysis.Spry2,ERK and phospho-ERK expression in HCC cell lines was detected by Western blotting.RESULTS:Among the patients,86.3%(207 of 240) exhibited down-regulation of Spry2 expression.Patients negative for Spry2 showed poorer survival(P=0.002) and increased recurrence(P=0.003).Multivariate analysis further established Spry2 as an independent predictor of postoperative recurrence in HCC patients(HR=1.47;95% CI,1.02-2.08;P=0.037).Downregulation of Spry2 was associated with highly malignant phenotypes like vascular invasion and advanced tumor stages,and was positively correlated with the metastatic potential of HCC cell lines.CONCLUSION:In the era of molecular targeted therapy,the expression of Spry2 in HCC may have relevant clinical significance and turn out to be a key factor in prognostic assessment and in treatment planning.
