In recent years, sweet potato virus disease （SPVD） has severely affected the production of sweet potato in China. In order to select chemical agents to prevent sweet potato from being infected with SPVD, 14 pesticid...In recent years, sweet potato virus disease （SPVD） has severely affected the production of sweet potato in China. In order to select chemical agents to prevent sweet potato from being infected with SPVD, 14 pesticides were sprayed on the plants of sweet potato infected with SPVD, and the relative mRNA level of the viruses in the leaves and physiological indicators of sweet potato plants were detected. The results showed that after the application of most of the pesticides, the relative mRNA level of the two viruses that caused SPVD decreased, and the chlorophyll content and biomass of sweet potato infected with SPVD increased significantly or extremely significantly. Among the pesticides, BASF virus liquid, 0.1% physcion and Aolike powder for cucumber virus were proved to be the best, as the chlorophyll content and stem length of sweet potato increased significantly. The experiment indicated that the pesticides could reduce the harm of SPVD to sweet potato.展开更多
As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)t...As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)technology to effectively up-regulate the expression of laccase(Ib LACs)by successfully inhibiting the expression of mi R397.The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation,including phenylalanine ammonialyase(PAL),4-coumarate-Co Aligase(4 CL),hydroxycinnamoyl Co A:shikimatetransferase(HTC),caffeicacid O-methyltransferase(COMT),and cinnamyl alcohol dehydrogenase(CAD).Meanwhile,the activities of PAL and LAC increased significantly,finally leading to increased lignin content.Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants,reduced the accumulation of SPVD transmitted by Bemisia tabaci(Gennadius),and promoted healthy sweetpotato growth.The results provide new insights for disease resistance breeding and green production of sweetpotato.展开更多
基金Supported by Construction Program of Culitvation Post for Mid-lower Reaches of Yangtze River of Modern Agricultural Industry Technology System(nycytx-16-B-13)Program from Nanchong Experimental Station of Modern Agricultural Industry Technology System(nycytx-16-c-16)Program of Sichuan-Chongqing Potato and Soybean Observation Station of Ministry of Agriculture~~
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund(CX(17)2023)Special Fund for Construction of Modern Agricultural Industrial Technology System(CARS-11-B-03)
文摘In recent years, sweet potato virus disease （SPVD） has severely affected the production of sweet potato in China. In order to select chemical agents to prevent sweet potato from being infected with SPVD, 14 pesticides were sprayed on the plants of sweet potato infected with SPVD, and the relative mRNA level of the viruses in the leaves and physiological indicators of sweet potato plants were detected. The results showed that after the application of most of the pesticides, the relative mRNA level of the two viruses that caused SPVD decreased, and the chlorophyll content and biomass of sweet potato infected with SPVD increased significantly or extremely significantly. Among the pesticides, BASF virus liquid, 0.1% physcion and Aolike powder for cucumber virus were proved to be the best, as the chlorophyll content and stem length of sweet potato increased significantly. The experiment indicated that the pesticides could reduce the harm of SPVD to sweet potato.
基金financially supported by the National Key R&D Program of China (2019YFD1001300 and 2019YFD1001305)the earmarked fund for CARS-10-Sweetpotatothe Jiangsu Postgraduate Scientific Research and Practical Innovation Program Project, China (KYCX19-2207)
文摘As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)technology to effectively up-regulate the expression of laccase(Ib LACs)by successfully inhibiting the expression of mi R397.The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation,including phenylalanine ammonialyase(PAL),4-coumarate-Co Aligase(4 CL),hydroxycinnamoyl Co A:shikimatetransferase(HTC),caffeicacid O-methyltransferase(COMT),and cinnamyl alcohol dehydrogenase(CAD).Meanwhile,the activities of PAL and LAC increased significantly,finally leading to increased lignin content.Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants,reduced the accumulation of SPVD transmitted by Bemisia tabaci(Gennadius),and promoted healthy sweetpotato growth.The results provide new insights for disease resistance breeding and green production of sweetpotato.
