目的:考察济阴颗粒对雌性冠心病大鼠脂代谢通路因子小窝蛋白1(caveolin-1,Cav-1)、低密度脂蛋白受体(low density lipoprotein receptor,LDLR)、极低密度脂蛋白受体(very low density lipoprotein receptor,VLDLR)、ATP结合盒转运蛋白...目的:考察济阴颗粒对雌性冠心病大鼠脂代谢通路因子小窝蛋白1(caveolin-1,Cav-1)、低密度脂蛋白受体(low density lipoprotein receptor,LDLR)、极低密度脂蛋白受体(very low density lipoprotein receptor,VLDLR)、ATP结合盒转运蛋白家族转运蛋白A1(atp-binding cassette transporter a1,ABCA1)、载脂蛋白A1(apolipoprotein-a1,ApoA1)、B1类清道夫受体(scavenger receptor class B type1,SRB1)mRNA水平、血清总胆固醇含量的影响。方法:健康雌性SD大鼠75只,除空白组15只外,其余60只制备绝经期冠心病模型,分为模型组、西药对照组、中药对照组、济阴颗粒组,每组15只。分离心脏主动脉,Elisa法测定各组大鼠血清总胆固醇含量,实时定量PCR方法检测雌性冠心病大鼠CAV1、LDLR、VLDLR、ABCA1、ApoA1、SRB1 mRNA水平。结果:实时定量PCR结果显示,同模型组比较,空白组、西药阳性组、中药阳性组、济阴颗粒组ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA表达增加,差异有统计学意义(P<0.05)。与空白组相比,济阴颗粒组ApoA1、CAV1、SRB1、VLDLR mRNA表达明显减少,差异有统计学意义(P<0.01),济阴颗粒组ABCA1、LDLR mRNA表达差异无统计学意义。ELISA结果显示,与模型组比较,空白组、西药阳性组、中药阳性组总胆固醇含量显著减少,差异有统计学意义(P<0.01),济阴颗粒组总胆固醇含量减少,具有统计学意义(P<0.05)。结论:济阴颗粒可能通过降低去卵巢雌性冠心病大鼠总胆固醇含量,提高ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA相对表达量来改善雌性大鼠冠心病。展开更多
Inhibition of atherogenesis through inhibition of lipid metabolism has not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done. Inhibition of atherogenesis v...Inhibition of atherogenesis through inhibition of lipid metabolism has not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done. Inhibition of atherogenesis via inhibition of lipid metabolism can be done through the mechanism of Reverse Cholesterol Transport (RCT). Signaling pathways that play a role in this mechanism is LXR signaling. LXR activation by an LXR agonist led increasing cholesterol efflux. Catechins based on bioinformatics study showed as a potent candidate LXR agonists that can be used as an inhibitor of atherogenesis. This study aims to prove that the administration Catechins green tea Clone GMB4 can prevent atherosclerosis through increasing mechanism cholesterol efflux from macrophage by taking effect of ABCA1, ABCG1, SRB1 gene expression in cultured macrophages were exposed ox-LDL. Long-term goals of the outcome of the research are the use of Catechins Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used as a complementary therapy for the treatment of atherosclerosis and cardiovascular diseases. The research is divided into 5 groups, namely the culture of macrophages without exposed ox-LDL, culture exposed ox-LDL and groups of Catechins dose I, II, III. In vitro study showed that administration of Catechins increases mRNA of ABCA1, whereas mRNA ABCG1 and SRB1 decreased at all three doses given. The result of protein profilling was identified a protein with a molecular weight of 70 kDa by SDS-PGE with silver staining.展开更多
文摘目的:考察济阴颗粒对雌性冠心病大鼠脂代谢通路因子小窝蛋白1(caveolin-1,Cav-1)、低密度脂蛋白受体(low density lipoprotein receptor,LDLR)、极低密度脂蛋白受体(very low density lipoprotein receptor,VLDLR)、ATP结合盒转运蛋白家族转运蛋白A1(atp-binding cassette transporter a1,ABCA1)、载脂蛋白A1(apolipoprotein-a1,ApoA1)、B1类清道夫受体(scavenger receptor class B type1,SRB1)mRNA水平、血清总胆固醇含量的影响。方法:健康雌性SD大鼠75只,除空白组15只外,其余60只制备绝经期冠心病模型,分为模型组、西药对照组、中药对照组、济阴颗粒组,每组15只。分离心脏主动脉,Elisa法测定各组大鼠血清总胆固醇含量,实时定量PCR方法检测雌性冠心病大鼠CAV1、LDLR、VLDLR、ABCA1、ApoA1、SRB1 mRNA水平。结果:实时定量PCR结果显示,同模型组比较,空白组、西药阳性组、中药阳性组、济阴颗粒组ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA表达增加,差异有统计学意义(P<0.05)。与空白组相比,济阴颗粒组ApoA1、CAV1、SRB1、VLDLR mRNA表达明显减少,差异有统计学意义(P<0.01),济阴颗粒组ABCA1、LDLR mRNA表达差异无统计学意义。ELISA结果显示,与模型组比较,空白组、西药阳性组、中药阳性组总胆固醇含量显著减少,差异有统计学意义(P<0.01),济阴颗粒组总胆固醇含量减少,具有统计学意义(P<0.05)。结论:济阴颗粒可能通过降低去卵巢雌性冠心病大鼠总胆固醇含量,提高ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA相对表达量来改善雌性大鼠冠心病。
文摘Inhibition of atherogenesis through inhibition of lipid metabolism has not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done. Inhibition of atherogenesis via inhibition of lipid metabolism can be done through the mechanism of Reverse Cholesterol Transport (RCT). Signaling pathways that play a role in this mechanism is LXR signaling. LXR activation by an LXR agonist led increasing cholesterol efflux. Catechins based on bioinformatics study showed as a potent candidate LXR agonists that can be used as an inhibitor of atherogenesis. This study aims to prove that the administration Catechins green tea Clone GMB4 can prevent atherosclerosis through increasing mechanism cholesterol efflux from macrophage by taking effect of ABCA1, ABCG1, SRB1 gene expression in cultured macrophages were exposed ox-LDL. Long-term goals of the outcome of the research are the use of Catechins Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used as a complementary therapy for the treatment of atherosclerosis and cardiovascular diseases. The research is divided into 5 groups, namely the culture of macrophages without exposed ox-LDL, culture exposed ox-LDL and groups of Catechins dose I, II, III. In vitro study showed that administration of Catechins increases mRNA of ABCA1, whereas mRNA ABCG1 and SRB1 decreased at all three doses given. The result of protein profilling was identified a protein with a molecular weight of 70 kDa by SDS-PGE with silver staining.
