This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis....This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis. Using 12 pairs of SSR primers,a total of 47 allelic variants were detected in 13 inbred lines,2-5 alleles were detected for each pair of primers,an average of 3. 9,and polymorphism information content varied from 0. 379 to 0. 828. According to the cluster analysis,the 13 inbred lines could be divided into 5 groups.展开更多
Five super hybrid rice combinations, i.e. HYS-1/R105, Pei'ai 64S/E32, Liangyoupeijiu (Pei'ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SS...Five super hybrid rice combinations, i.e. HYS-1/R105, Pei'ai 64S/E32, Liangyoupeijiu (Pei'ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributed on 12 rice chromosomes were used, out of which 47 detected polymorphism among the tested rice lines. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish among most rice genotypes tested. Twenty-four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker-based fingerprinting for the rice lines. For most of the primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F1 from its parental lines. In addition, 5 primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification.展开更多
As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined ...As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined deeply,so it is urgent to improve and strengthen the purity and viability of "Huangzhou radish" including bad phenomena such as degeneration of varieties after self-pollination.At present,there was no SSR molecular marker can be used for genetic diversity identification of "Huangzhou radish".SSR molecular marker can provide a favorable tool to study "Huangzhou radish".In this study,"Xiakang","Hongbao"and "Huangzhou radish" were used as experimental materials,100 SSR primers were screened,and 25 pairs of SSR primers were selected.Finally,two pairs of primers were selected for identification of the purity of "Huangzhou radish" by non-denatured polyacrylamide gel electrophoresis (PAGE).The results showed that R-102 and R-112 primer pairs could amplify the special bands for "Huangzhou radish" from above three kinds of radishes.These two pairs of primers could be developed as SSR markers to identify the specificity and purity of "Huangzhou radish".Furthermore,this study provides convenient conditions for promoting the economic and social value of "Huangzhou radish".展开更多
SSR molecular markers were used to analyze the genetic diversity of 8 Jinsha pomelo germplasm and its closely-related varieties from main planting area in Jiangxi Province. A total of 92 alleles were detected from 26 ...SSR molecular markers were used to analyze the genetic diversity of 8 Jinsha pomelo germplasm and its closely-related varieties from main planting area in Jiangxi Province. A total of 92 alleles were detected from 26 pairs of selected primers with 53. 68% of polymorphism. The alleles detected per locus were ranged from 2 to 8. On average,3. 54 alleles were amplified from per pair of primers. The similarity coefficients of the 8 varieties were in the range of 0. 729-0. 924.According to the genetic similarities based on UPGMA method,all materials were divided into two groups of Jinlan pomelo and Shatian pomelo at the similarity coefficient of 0. 738.展开更多
This paper reports the development of the first SSR marker-based sugarcane (Saccharum spp.) molecular identity database in the world. Since 2005, 1,025 sugarcane clones were genotyped, including 811 Louisiana, 45 Flor...This paper reports the development of the first SSR marker-based sugarcane (Saccharum spp.) molecular identity database in the world. Since 2005, 1,025 sugarcane clones were genotyped, including 811 Louisiana, 45 Florida, 39 Texas, 130 foreign, and eight consultant/seed company clones. Genotyping was done on a fluorescence-capillary electrophoresis detection platform involving 21 highly polymorphic SSR markers that could potentially amplify 144 distinctive DNA fragments. Genotyping data were processed with the GeneMapper? software to reveal electrophoregrams that were manually checked against the 144 fragments. The presence (A) or absence (C) of these 144 fragments in any sugarcane clone was recorded in an affixed sequence order as a DNAMAN? file to represent its molecular identity being achieved into a local molecular identity database. The molecular identity database has been updated annually by continued genotyping of newly assigned sugarcane clones. The database provides molecular descriptions for new cultivar registration articles, enables sugarcane breeders to identify mis-labeled sugarcane clones in crossing programs and determine the paternity of cross progeny, and ensures the desired cultivars are grown in farmers’ fields.展开更多
文摘This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis. Using 12 pairs of SSR primers,a total of 47 allelic variants were detected in 13 inbred lines,2-5 alleles were detected for each pair of primers,an average of 3. 9,and polymorphism information content varied from 0. 379 to 0. 828. According to the cluster analysis,the 13 inbred lines could be divided into 5 groups.
文摘Five super hybrid rice combinations, i.e. HYS-1/R105, Pei'ai 64S/E32, Liangyoupeijiu (Pei'ai 64S/9311), 88S/0293, and J23A/Q611, and their parental lines were tested by means of SSR analysis. A total of 144 SSR primer pairs distributed on 12 rice chromosomes were used, out of which 47 detected polymorphism among the tested rice lines. Among all these primers, RM337 and RM154 produced polymorphic patterns in four or more of the tested experimental materials respectively, and they could distinguish among most rice genotypes tested. Twenty-four primer pairs, two on each rice chromosome, were selected to make a reference SSR marker-based fingerprinting for the rice lines. For most of the primer pairs, F1 hybrids mainly showed complementary pattern of both parents, which could be very useful to distinguish the F1 from its parental lines. In addition, 5 primer pairs were selected as special primer pairs for five hybrid rice combinations respectively. By combining the rapid, simple method on DNA extraction, it is suggested that SSR technique has wide prospective in variety authentication and purity identification.
基金Supported by the Hubei Special Project for Development of Science and Technology in Local by Central Guidance(2018ZYYD019)
文摘As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined deeply,so it is urgent to improve and strengthen the purity and viability of "Huangzhou radish" including bad phenomena such as degeneration of varieties after self-pollination.At present,there was no SSR molecular marker can be used for genetic diversity identification of "Huangzhou radish".SSR molecular marker can provide a favorable tool to study "Huangzhou radish".In this study,"Xiakang","Hongbao"and "Huangzhou radish" were used as experimental materials,100 SSR primers were screened,and 25 pairs of SSR primers were selected.Finally,two pairs of primers were selected for identification of the purity of "Huangzhou radish" by non-denatured polyacrylamide gel electrophoresis (PAGE).The results showed that R-102 and R-112 primer pairs could amplify the special bands for "Huangzhou radish" from above three kinds of radishes.These two pairs of primers could be developed as SSR markers to identify the specificity and purity of "Huangzhou radish".Furthermore,this study provides convenient conditions for promoting the economic and social value of "Huangzhou radish".
基金Supported by Natural Science Foundation of China(31501718,31460508)Scientific Research of Doctor Start-up Fund Project of Jiangxi Academy of Agricultural Sciences(20142CBS008)+3 种基金Jiangxi Province Foreign Science and Technology Cooperation Project(20141BDH80005)National International Scientific and Technological Cooperation Program(2015DFA31060)Innovation Fund of Jiangxi Academy of Agricultural sciences(2013CJJ009,2016CJJ002)Guangxi Innovation Team Nanning Test Station Project of Citrus Industry National Modern Agriculture Technology System(nycytxgxcxtd-05-08)
文摘SSR molecular markers were used to analyze the genetic diversity of 8 Jinsha pomelo germplasm and its closely-related varieties from main planting area in Jiangxi Province. A total of 92 alleles were detected from 26 pairs of selected primers with 53. 68% of polymorphism. The alleles detected per locus were ranged from 2 to 8. On average,3. 54 alleles were amplified from per pair of primers. The similarity coefficients of the 8 varieties were in the range of 0. 729-0. 924.According to the genetic similarities based on UPGMA method,all materials were divided into two groups of Jinlan pomelo and Shatian pomelo at the similarity coefficient of 0. 738.
文摘This paper reports the development of the first SSR marker-based sugarcane (Saccharum spp.) molecular identity database in the world. Since 2005, 1,025 sugarcane clones were genotyped, including 811 Louisiana, 45 Florida, 39 Texas, 130 foreign, and eight consultant/seed company clones. Genotyping was done on a fluorescence-capillary electrophoresis detection platform involving 21 highly polymorphic SSR markers that could potentially amplify 144 distinctive DNA fragments. Genotyping data were processed with the GeneMapper? software to reveal electrophoregrams that were manually checked against the 144 fragments. The presence (A) or absence (C) of these 144 fragments in any sugarcane clone was recorded in an affixed sequence order as a DNAMAN? file to represent its molecular identity being achieved into a local molecular identity database. The molecular identity database has been updated annually by continued genotyping of newly assigned sugarcane clones. The database provides molecular descriptions for new cultivar registration articles, enables sugarcane breeders to identify mis-labeled sugarcane clones in crossing programs and determine the paternity of cross progeny, and ensures the desired cultivars are grown in farmers’ fields.
