[Objective] This study aimed to establish a high-efficiency and stable SSR amplification system and screen polymorphic primers in order to further compare the tri-group probability analysis method and traditional QTL ...[Objective] This study aimed to establish a high-efficiency and stable SSR amplification system and screen polymorphic primers in order to further compare the tri-group probability analysis method and traditional QTL mapping method. [Method] In this study, we preliminarily screened the 605 pairs of primers evenly distributed on the 12 chromosomes through investigating their polymorphism performance in amplification, and established an optimized SSR-PCR reaction system. [Result] A 10μL SSR-PCR reaction system suitable for rice was set up as fol ows： 2 μl of 10 × Buffer, 2.0 mmol/L Mg2＋ （final concentration）, 0.5 mmol/L dNTPs, 1.0 μmol/L primers （final concentration）, 1 μl of DNA template, 0.15 U Taq DNA polymerase. Among the SSR primers distributed over the genome, 142 pairs that were polymorphic upon the parents were screened. [Conclusion] This study lays a good foundation for sub-sequent QTL mapping studies.展开更多
Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showe...Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showed that 493 discernible loci with distinct elec- trophoretic bands were obtained, of which, 478 loci （96.78%） were polymorphic. This indicated that oil-tea germplasms possess abundant genetic diversities. By clustering analysis performed using softwares of NTSYS 2.10 and Winboot, 51 oil-tea germplasms were divided into two groups： Group I had 48 lines of Camellia oleifera Abel, while Group II had three C. oleifera Abel related species and their similarity coefficient was 0.62. Group I was further divided into Group I-1 and Group I-2, and their similarity coefficient （Gs） was 0.634. All members of Group I-1 originated from Hunan Province, while Group I-2 included the rest of Hunan lines and those originated from other regions of China. Analyzed by software POPGENE 1.32, the Shannon＇s information index （I＊） of genetic polymorphism was 0.3852, the genetic diversity among different region popula- tions （Ht） was 0.2537, the genetic diversity within populations （Hs） was 0.15545, the dif- ferentiation coefficient of genetic diversity among populations （Gst） was 0.3967, and the gene flow among populations （Nm＊） was 0.8262. The Nei＇s genetic distances between the Hunan population and the populations originated from other regions of China implied that geographic isolation strongly influenced genetic differentiation among populations. Meanwhile, seedling rootstock grafting and high grafting for tree crown produced genetic variations among clonal offsprings.展开更多
基金Supported by the Governor Special Fund for Excellent Talents for Education of Science and Technology of Guizhou Province(2012093025)~~
文摘[Objective] This study aimed to establish a high-efficiency and stable SSR amplification system and screen polymorphic primers in order to further compare the tri-group probability analysis method and traditional QTL mapping method. [Method] In this study, we preliminarily screened the 605 pairs of primers evenly distributed on the 12 chromosomes through investigating their polymorphism performance in amplification, and established an optimized SSR-PCR reaction system. [Result] A 10μL SSR-PCR reaction system suitable for rice was set up as fol ows： 2 μl of 10 × Buffer, 2.0 mmol/L Mg2＋ （final concentration）, 0.5 mmol/L dNTPs, 1.0 μmol/L primers （final concentration）, 1 μl of DNA template, 0.15 U Taq DNA polymerase. Among the SSR primers distributed over the genome, 142 pairs that were polymorphic upon the parents were screened. [Conclusion] This study lays a good foundation for sub-sequent QTL mapping studies.
文摘Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showed that 493 discernible loci with distinct elec- trophoretic bands were obtained, of which, 478 loci （96.78%） were polymorphic. This indicated that oil-tea germplasms possess abundant genetic diversities. By clustering analysis performed using softwares of NTSYS 2.10 and Winboot, 51 oil-tea germplasms were divided into two groups： Group I had 48 lines of Camellia oleifera Abel, while Group II had three C. oleifera Abel related species and their similarity coefficient was 0.62. Group I was further divided into Group I-1 and Group I-2, and their similarity coefficient （Gs） was 0.634. All members of Group I-1 originated from Hunan Province, while Group I-2 included the rest of Hunan lines and those originated from other regions of China. Analyzed by software POPGENE 1.32, the Shannon＇s information index （I＊） of genetic polymorphism was 0.3852, the genetic diversity among different region popula- tions （Ht） was 0.2537, the genetic diversity within populations （Hs） was 0.15545, the dif- ferentiation coefficient of genetic diversity among populations （Gst） was 0.3967, and the gene flow among populations （Nm＊） was 0.8262. The Nei＇s genetic distances between the Hunan population and the populations originated from other regions of China implied that geographic isolation strongly influenced genetic differentiation among populations. Meanwhile, seedling rootstock grafting and high grafting for tree crown produced genetic variations among clonal offsprings.
