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观赏羽衣甘蓝SSR标记分型与亲缘关系研究 被引量:9
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作者 郭宁 高怀杰 +4 位作者 韩硕 宗梅 王桂香 张月云 刘凡 《植物遗传资源学报》 CAS CSCD 北大核心 2017年第2期349-357,358,共10页
观赏羽衣甘蓝凭借优良的观赏特性和抗逆性已经成为重要的冷季观赏植物。国内观赏羽衣甘蓝育种起步较晚,并且缺乏对种质资源遗传背景的系统研究。本研究应用SSR标记对不同类型的观赏羽衣甘蓝材料进行标记分型和亲缘关系分析。从99对均匀... 观赏羽衣甘蓝凭借优良的观赏特性和抗逆性已经成为重要的冷季观赏植物。国内观赏羽衣甘蓝育种起步较晚,并且缺乏对种质资源遗传背景的系统研究。本研究应用SSR标记对不同类型的观赏羽衣甘蓝材料进行标记分型和亲缘关系分析。从99对均匀分布于甘蓝基因组的SSR引物中筛选出46对多态性好的引物,对27份不同类型的观赏羽衣甘蓝材料进行标记分型,共扩增出210个多态性位点,平均PIC值为0.58。进一步利用标记分型结果进行STRUCTURE群体结构、UPGMA聚类和聚类热图分析,结果显示3种分析结果基本一致,可以将27份材料分为圆叶、羽叶和皱叶3种类型,其中圆叶和羽叶类型的亲缘关系更近,与皱叶类型的亲缘关系较远;STRUCTURE分析还可以将双亲为不同类型的杂交种材料进行区分;聚类热图分析可以将标记分型结果形象的展示出来。本研究为进一步建立观赏羽衣甘蓝分子指纹图谱,明确种质资源的遗传背景,建立观赏羽衣甘蓝分子标记辅助选择育种体系,培育具有自主知识产权的新品种奠定基础。 展开更多
关键词 观赏羽衣甘蓝 ssr标记分型 群体结构 聚类分析 亲缘关系
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Molecular Characterization of Olive Cultivars in Iraq Using SSR Markers and Compare with Phenotypic Characterization
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作者 Iqbal Harbi Salwa Jaber A1-Awadi Ali Imad Mohammad Moner 《Journal of Life Sciences》 2012年第12期1343-1350,共8页
Abstract: Simple sequence repeat (SSR) analysis was used to study the genotype relation among ten different olives varieties from Al-Zafrania and A1-Mosel station ministry of agriculture/Iraq Shami, Sorani, Manzenl... Abstract: Simple sequence repeat (SSR) analysis was used to study the genotype relation among ten different olives varieties from Al-Zafrania and A1-Mosel station ministry of agriculture/Iraq Shami, Sorani, Manzenllo, Qaysi, Arbqween, Jlot (Labeeb), Baashiqi, Dahkan, Nepali, Khodeiri, Fifteen SSR loci were studied and produced 239 amplified fragment. Two hundred and thirty seven of these loci (99.16%) were polymorphic over all the genotypes tested. Dendrogram and matrix of similarity were obtained by the Unweighted Pair-Group Method analysis (UPGMA). Study showed two groups: group A: Nepali, Arbqween, and group B: divided in two sub groups (sub group B 1: Jlot, Dahkan, sub group B2: other cultivar), while the genotype relation according to phenotype was confused. SSR has a better molecular marker than other molecular technique for detecting genetic relationship among cultivars, and help in known the pedigree of relatives and ancestors. 展开更多
关键词 OLIVE PCR ssr molecular markers.
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