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STK/Connect模块在GPS仿真演示系统中的应用 被引量:10
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作者 潘成胜 张馨 李定主 《火力与指挥控制》 CSCD 北大核心 2008年第10期117-120,共4页
卫星工具软件STK(Satellite Tool Kit)是航天领域中进行卫星系统分析和仿真的有效工具,其重要模块之一STK/Connect可提供用户在客户机/服务器环境下与STK连接的功能。在深入研究STK/Connect模块的核心技术的基础上,详细说明了第三方应... 卫星工具软件STK(Satellite Tool Kit)是航天领域中进行卫星系统分析和仿真的有效工具,其重要模块之一STK/Connect可提供用户在客户机/服务器环境下与STK连接的功能。在深入研究STK/Connect模块的核心技术的基础上,详细说明了第三方应用程序与STK进行通信的方法,并将其应用于GPS应用仿真演示系统,同时进行了编程实现,取得了较好的效果。 展开更多
关键词 卫星工具软件 stk/connect GPS
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STK/Connect模块分析与应用 被引量:26
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作者 张云彬 张永生 《测绘学院学报》 北大核心 2001年第B09期29-32,共4页
STK的重要模块之一STK/Connect提供用户在客户机 /服务器环境下与STK连接的功能 ,根据作者的实践 。
关键词 stk 客户机/服务器 卫星工具软件 连接库 可视化 航天遥感
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基于STK的Connect模块设计与应用 被引量:12
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作者 李弢 陈立云 +1 位作者 连云峰 高秀峰 《科学技术与工程》 2008年第22期6110-6112,6121,共4页
卫星工具软件(STK)是广泛用于航天工程全过程的先进卫星系统分析软件,具有准确的仿真能力,但在实际应用中经常遇到STK与应用程序的集成问题。介绍了利用STK/Connect模块实现STK与VC集成的方法,并通过一个通信卫星链路的应用实例详细说... 卫星工具软件(STK)是广泛用于航天工程全过程的先进卫星系统分析软件,具有准确的仿真能力,但在实际应用中经常遇到STK与应用程序的集成问题。介绍了利用STK/Connect模块实现STK与VC集成的方法,并通过一个通信卫星链路的应用实例详细说明了两者联合编程的步骤。两者的集成有效地利用了STK准确的仿真能力与VC高效的数据分析能力,提高了仿真分析系统开发的灵活性。 展开更多
关键词 卫星工具软件 connect模块 仿真
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基于C# Connect连接的STK远程控制系统设计与实现 被引量:4
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作者 霍烁烁 刘镇瑜 +2 位作者 陈磊 赵文杰 钱苏敏 《舰船电子工程》 2017年第11期102-105,共4页
STK(卫星仿真工具包)已成为系统仿真领域重要的工具软件,其覆盖雷达、通信、光电等领域的仿真开发能力为各类系统仿真开发提供了强大的支撑。STK Connect模块为基于网络通信的STK二次开发提供了高效的实现方法。论文深入研究了基于C#的S... STK(卫星仿真工具包)已成为系统仿真领域重要的工具软件,其覆盖雷达、通信、光电等领域的仿真开发能力为各类系统仿真开发提供了强大的支撑。STK Connect模块为基于网络通信的STK二次开发提供了高效的实现方法。论文深入研究了基于C#的STK Connect连接方法,提出一种基于此的STK远程控制系统软件的设计方法,实现了对STK的通用驱动。 展开更多
关键词 stk connect C# 二次开发
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改进RRT-Connect与DWA算法的巡检机器人路径规划研究 被引量:1
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作者 罗征志 韩怡可 +1 位作者 张鑫 邹宇博 《计算机工程与应用》 CSCD 北大核心 2024年第15期344-354,共11页
针对传统RRT-Connect算法在密集复杂环境中路径规划效率低、动态避障效果差等问题,提出一种改进RRT-Connect与DWA融合算法。该算法通过改进采样策略、动态步长优化和碰撞检测引导随机树生长;在随机树中采用贪心策略和角度约束优化路径... 针对传统RRT-Connect算法在密集复杂环境中路径规划效率低、动态避障效果差等问题,提出一种改进RRT-Connect与DWA融合算法。该算法通过改进采样策略、动态步长优化和碰撞检测引导随机树生长;在随机树中采用贪心策略和角度约束优化路径。基于巡检机器人建立运动学模型,通过速度采样空间生成轨迹簇;建立模糊逻辑系统自适应调整DWA算法评价函数的权重系数,将全局最优路径点融入DWA算法中实现全局最优路径和实时避障。仿真结果表明,在油气站场密集复杂环境中,改进RRT-Connect算法较传统算法路径缩短约27.09%,平滑度提高约84.6%,碰撞距离提高约18.75%;改进融合算法路径减少约2.97%,平滑度提高约78.8%,碰撞距离提高约30.6%,验证了提出算法的有效性。 展开更多
关键词 路径规划 改进RRT-connect算法 DWA算法 融合算法
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STK/Connect在态势模拟系统中的应用研究
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作者 王甲 姜希 《电子世界》 2016年第7期92-93,共2页
针对现有态势模拟系统仿真度低、成本高昂等问题,提出了一种基于STK/Connect的态势模拟系统。该系统通过单元与STK/Connect中间件耦合的方式,构建出具有扩展性强、灵活快捷特点的态势模拟系统,对战场快速模拟与推演具有较强的应用价值。
关键词 态势模拟 stk stk/connect组件 中间件
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基于逆向重布线改进RRT-Connect算法的机械臂路径规划
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作者 王博 顾寄南 李兴家 《江苏科技大学学报(自然科学版)》 CAS 2024年第2期47-52,共6页
为解决RRT-Connect算法在复杂非结构环境下规划出的路径成本较长、有效节点数过多、曲折不平滑等问题,提出了一种基于逆向重布线的改进RRT-Connect算法(Improved-RRT-Connect).首先,在算法的起始位置加入碰撞检测函数,当起点和终点之间... 为解决RRT-Connect算法在复杂非结构环境下规划出的路径成本较长、有效节点数过多、曲折不平滑等问题,提出了一种基于逆向重布线的改进RRT-Connect算法(Improved-RRT-Connect).首先,在算法的起始位置加入碰撞检测函数,当起点和终点之间不存在障碍物时,能够快速生成一条路径;其次,通过对生成的路径采取逆向重布线的措施,对生成的路径中的节点进行重新筛选布线,达到缩短生成路径长度,减少有效节点数量的目的;最后,通过结合B样条曲线对生成的路径进行拟合处理,得到一条平滑连续的路径.通过Improved-RRT-Connect算法与RRT、RRT^(*)-Smart、RRT-Connect等算法的对比实验表明,在规划时间上,Improved-RRT-Connect算法比RRT、RRT^(*)-Smart算法减少了52.14%、98.53%,在路径成本上比RRT、RRT-Connect算法减少了19.39%、17.15%,阐明了提出的Improved-RRT-Connect算法的优越性. 展开更多
关键词 RRT-connect算法 机械臂 碰撞检测 逆向重布线 B样条曲线
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基于改进RRT-Connect算法的全局路径规划
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作者 朱建军 王明森 《组合机床与自动化加工技术》 北大核心 2024年第4期52-55,61,共5页
针对RRT-Connect算法在复杂环境内的路径规划中存在探索性弱、收敛速度慢、冗余节点多、搜索路径较长等问题,提出一种改进的RRT-Connect算法。通过引入高质量随机点和动态步长的方法,提高了生成随机树的质量并减少了冗余节点数量;采用... 针对RRT-Connect算法在复杂环境内的路径规划中存在探索性弱、收敛速度慢、冗余节点多、搜索路径较长等问题,提出一种改进的RRT-Connect算法。通过引入高质量随机点和动态步长的方法,提高了生成随机树的质量并减少了冗余节点数量;采用正向寻优和逆向贪婪的方式,改善了搜索路径较长的问题。实验结果表明,改进RRT-Connect算法平均路径规划时间缩短26.41%,平均路径规划长度缩短19.05%,平均路径规划节点个数减少41.91%,证明了改进RRT-Connect算法相比于原算法规划效率更高,规划时间更少,规划路径质量更优。 展开更多
关键词 路径规划 RRT-connect算法 动态步长 轨迹优化 贪婪算法 3次B样条曲线
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Ensuring Secure Platooning of Constrained Intelligent and Connected Vehicles Against Byzantine Attacks:A Distributed MPC Framework 被引量:1
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作者 Henglai Wei Hui Zhang +1 位作者 Kamal AI-Haddad Yang Shi 《Engineering》 SCIE EI CAS CSCD 2024年第2期35-46,共12页
This study investigates resilient platoon control for constrained intelligent and connected vehicles(ICVs)against F-local Byzantine attacks.We introduce a resilient distributed model-predictive platooning control fram... This study investigates resilient platoon control for constrained intelligent and connected vehicles(ICVs)against F-local Byzantine attacks.We introduce a resilient distributed model-predictive platooning control framework for such ICVs.This framework seamlessly integrates the predesigned optimal control with distributed model predictive control(DMPC)optimization and introduces a unique distributed attack detector to ensure the reliability of the transmitted information among vehicles.Notably,our strategy uses previously broadcasted information and a specialized convex set,termed the“resilience set”,to identify unreliable data.This approach significantly eases graph robustness prerequisites,requiring only an(F+1)-robust graph,in contrast to the established mean sequence reduced algorithms,which require a minimum(2F+1)-robust graph.Additionally,we introduce a verification algorithm to restore trust in vehicles under minor attacks,further reducing communication network robustness.Our analysis demonstrates the recursive feasibility of the DMPC optimization.Furthermore,the proposed method achieves exceptional control performance by minimizing the discrepancies between the DMPC control inputs and predesigned platoon control inputs,while ensuring constraint compliance and cybersecurity.Simulation results verify the effectiveness of our theoretical findings. 展开更多
关键词 Model predictive control Resilient control Platoon control Intelligent and connected vehicle Byzantine attacks
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A divergent pattern in functional connectivity: a transdiagnostic perspective
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作者 Lu Zhang Lorenzo Pini 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第9期1885-1886,共2页
Functional magnetic resonance imaging(fMRI)is a popular tool used to investigate not only how the brain responds to specific stimuli during sensorimotor or cognitive tasks,but also brain activity at rest.The physics b... Functional magnetic resonance imaging(fMRI)is a popular tool used to investigate not only how the brain responds to specific stimuli during sensorimotor or cognitive tasks,but also brain activity at rest.The physics beyond this approach is based on the analysis of the blood oxygenation level-dependent signal. 展开更多
关键词 PATTERN connectIVITY FUNCTIONAL
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基于STK的反导预警雷达作战场景仿真分析方法
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作者 张浩 史新虎 《空天预警研究学报》 CSCD 2024年第1期61-64,共4页
针对有先验信息的类弹道导弹发射活动,提出了一种基于卫星工具软件(STK)的反导预警雷达作战场景仿真分析方法,利用STK软件可视化设计的优势,对反导预警雷达拦截搜索屏进行设计,并对搜索屏的拦截效果进行评估.这对进行雷达总体设计的相... 针对有先验信息的类弹道导弹发射活动,提出了一种基于卫星工具软件(STK)的反导预警雷达作战场景仿真分析方法,利用STK软件可视化设计的优势,对反导预警雷达拦截搜索屏进行设计,并对搜索屏的拦截效果进行评估.这对进行雷达总体设计的相关人员,具有一定的实用价值. 展开更多
关键词 卫星工具软件 反导作战预案 反导预警雷达 拦截搜索屏
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Approximately Bi-Similar Symbolic Model for Discretetime Interconnected Switched System
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作者 Yang Song Yongzhuang Liu Wanqing Zhao 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第10期2185-2187,共3页
Dear Editor,This letter concerns the development of approximately bi-similar symbolic models for a discrete-time interconnected switched system(DT-ISS).The DT-ISS under consideration is formed by connecting multiple s... Dear Editor,This letter concerns the development of approximately bi-similar symbolic models for a discrete-time interconnected switched system(DT-ISS).The DT-ISS under consideration is formed by connecting multiple switched systems known as component switched systems(CSSs).Although the problem of constructing approximately bi-similar symbolic models for DT-ISS has been addressed in some literature,the previous works have relied on the assumption that all the subsystems of CSSs are incrementally input-state stable. 展开更多
关键词 APPROXIMATE SYMBOLIC connectED
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基于STK的卫星信号仿真在复杂环境GNSS滑坡监测中的应用
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作者 董颖略 《铁道勘察》 2024年第6期17-23,30,共8页
为更加有效地控制GNSS监测关键环节,提高GNSS自动化监测效率和监测精度,实现滑坡灾害的有效监测,提出一种基于卫星仿真工具包(STK)的GNSS监测站分析技术,对研究区内GNSS卫星定位性能进行分析。基于STK卫星轨道预报数据建立北斗卫星导航... 为更加有效地控制GNSS监测关键环节,提高GNSS自动化监测效率和监测精度,实现滑坡灾害的有效监测,提出一种基于卫星仿真工具包(STK)的GNSS监测站分析技术,对研究区内GNSS卫星定位性能进行分析。基于STK卫星轨道预报数据建立北斗卫星导航星座,用来模拟GNSS监测过程中的卫星运动轨迹和定位信号传输过程,使用无人机获取高精度数字高程模型用以模拟真实的地形变化,并建立一套GNSS监测设备部署方法。选取某滑坡区进行工程验证,通过该技术分析全天GNSS卫星覆盖时段和时长信息,其中MEO卫星的平均监测时长5.92 h、IGSO卫星的平均监测时长16.87h,可以满足实验区全时段有效监测;重点计算实验区各点EDOP、NDOP、VDOP及PDOP值并绘制导航精度分布,研究区内PDOP值在0.8915~0.8955之间,定位精度理想,通过精度分布可以确定103°19′E,36°6′N处定位精度最优。工程验证表明,提出的GNSS监测站分析技术可以有效获得研究区不同区域的GNSS导航卫星定位精度,为复杂环境下监测站的精细化部署提供科学参考。 展开更多
关键词 铁路 滑坡 stk GNSS监测仿真 导航精度因子 监测站选址
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Ultra Reliable Load-Aware Connection Management(LACM)Algorithm in WIA-FA Systems
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作者 Liu Gang Jiang Chunhao +3 位作者 Ren Xiaochun Fan Pingzhi Liang Chengchao Ma Zheng 《China Communications》 SCIE CSCD 2024年第8期142-161,共20页
The increasing demand for industrial automation and intelligence has put forward higher requirements for the reliability of industrial wireless communication technology.As an international standard based on 802.11,Wir... The increasing demand for industrial automation and intelligence has put forward higher requirements for the reliability of industrial wireless communication technology.As an international standard based on 802.11,Wireless networks for Industrial Automation-Factory Automation(WIA-FA)greatly improves the reliability in factory automation scenarios by Time Division Multiple Access(TDMA).However,in ultra-dense WIA-FA networks with mobile users,the basic connection management mechanism is inefficient.Most of the handover and resource management algorithms are all based on frequency division multiplexing,not suitable for the TDMA in the WIA-FA network.Therefore,we propose Load-aware Connection Management(LACM)algorithm to adjust the linkage and balance the load of access devices to avoid blocking and improve the reliability of the system.And then we simulate the algorithm to find the optimal settings of the parameters.After comparing with other existing algorithms,the result of the simulation proves that LACM is more efficient in reliability and maintains high reliability of more than 99.8%even in the ultra-dense moving scenario with 1500 field devices.Besides,this algorithm ensures that only a few signaling exchanges are required to ensure load bal-ancing,which is no more than 5 times,and less than half of the best state-of-the-art algorithm. 展开更多
关键词 connection management load-aware multi-connectivity reliability RETRANSMISSION WIAFA
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Research on Quantitative Identification of Three-Dimensional Connectivity of Fractured-Vuggy Reservoirs
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作者 Xingliang Deng Peng Cao +3 位作者 Yintao Zhang Yuhui Zhou Xiao Luo Liang Wang 《Energy Engineering》 EI 2024年第5期1195-1207,共13页
The fractured-vuggy carbonate oil resources in the western basin of China are extremely rich.The connectivity of carbonate reservoirs is complex,and there is still a lack of clear understanding of the development and ... The fractured-vuggy carbonate oil resources in the western basin of China are extremely rich.The connectivity of carbonate reservoirs is complex,and there is still a lack of clear understanding of the development and topological structure of the pore space in fractured-vuggy reservoirs.Thus,effective prediction of fractured-vuggy reservoirs is difficult.In view of this,this work employs adaptive point cloud technology to reproduce the shape and capture the characteristics of a fractured-vuggy reservoir.To identify the complex connectivity among pores,fractures,and vugs,a simplified one-dimensional connectivity model is established by using the meshless connection element method(CEM).Considering that different types of connection units have different flow characteristics,a sequential coupling calculation method that can efficiently calculate reservoir pressure and saturation is developed.By automatic history matching,the dynamic production data is fitted in real-time,and the characteristic parameters of the connection unit are inverted.Simulation results show that the three-dimensional connectivity model of the fractured-vuggy reservoir built in this work is as close as 90%of the fine grid model,while the dynamic simulation efficiency is much higher with good accuracy. 展开更多
关键词 Fractured-vuggy reservoir three-dimensional connectivity connection unit dynamic prediction automatic history matching
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Structural and functional connectivity of the whole brain and subnetworks in individuals with mild traumatic brain injury:predictors of patient prognosis
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作者 Sihong Huang Jungong Han +4 位作者 Hairong Zheng Mengjun Li Chuxin Huang Xiaoyan Kui Jun Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第7期1553-1558,共6页
Patients with mild traumatic brain injury have a diverse clinical presentation,and the underlying pathophysiology remains poorly understood.Magnetic resonance imaging is a non-invasive technique that has been widely u... Patients with mild traumatic brain injury have a diverse clinical presentation,and the underlying pathophysiology remains poorly understood.Magnetic resonance imaging is a non-invasive technique that has been widely utilized to investigate neuro biological markers after mild traumatic brain injury.This approach has emerged as a promising tool for investigating the pathogenesis of mild traumatic brain injury.G raph theory is a quantitative method of analyzing complex networks that has been widely used to study changes in brain structure and function.However,most previous mild traumatic brain injury studies using graph theory have focused on specific populations,with limited exploration of simultaneous abnormalities in structural and functional connectivity.Given that mild traumatic brain injury is the most common type of traumatic brain injury encounte red in clinical practice,further investigation of the patient characteristics and evolution of structural and functional connectivity is critical.In the present study,we explored whether abnormal structural and functional connectivity in the acute phase could serve as indicators of longitudinal changes in imaging data and cognitive function in patients with mild traumatic brain injury.In this longitudinal study,we enrolled 46 patients with mild traumatic brain injury who were assessed within 2 wee ks of injury,as well as 36 healthy controls.Resting-state functional magnetic resonance imaging and diffusion-weighted imaging data were acquired for graph theoretical network analysis.In the acute phase,patients with mild traumatic brain injury demonstrated reduced structural connectivity in the dorsal attention network.More than 3 months of followup data revealed signs of recovery in structural and functional connectivity,as well as cognitive function,in 22 out of the 46 patients.Furthermore,better cognitive function was associated with more efficient networks.Finally,our data indicated that small-worldness in the acute stage could serve as a predictor of longitudinal changes in connectivity in patients with mild traumatic brain injury.These findings highlight the importance of integrating structural and functional connectivity in unde rstanding the occurrence and evolution of mild traumatic brain injury.Additionally,exploratory analysis based on subnetworks could serve a predictive function in the prognosis of patients with mild traumatic brain injury. 展开更多
关键词 cognitive function CROSS-SECTION FOLLOW-UP functional connectivity graph theory longitudinal study mild traumatic brain injury prediction small-worldness structural connectivity subnetworks whole brain network
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FADSF:A Data Sharing Model for Intelligent Connected Vehicles Based on Blockchain Technology
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作者 Yan Sun Caiyun Liu +1 位作者 Jun Li Yitong Liu 《Computers, Materials & Continua》 SCIE EI 2024年第8期2351-2362,共12页
With the development of technology,the connected vehicle has been upgraded from a traditional transport vehicle to an information terminal and energy storage terminal.The data of ICV(intelligent connected vehicles)is ... With the development of technology,the connected vehicle has been upgraded from a traditional transport vehicle to an information terminal and energy storage terminal.The data of ICV(intelligent connected vehicles)is the key to organically maximizing their efficiency.However,in the context of increasingly strict global data security supervision and compliance,numerous problems,including complex types of connected vehicle data,poor data collaboration between the IT(information technology)domain and OT(operation technology)domain,different data format standards,lack of shared trust sources,difficulty in ensuring the quality of shared data,lack of data control rights,as well as difficulty in defining data ownership,make vehicle data sharing face a lot of problems,and data islands are widespread.This study proposes FADSF(Fuzzy Anonymous Data Share Frame),an automobile data sharing scheme based on blockchain.The data holder publishes the shared data information and forms the corresponding label storage on the blockchain.The data demander browses the data directory information to select and purchase data assets and verify them.The data demander selects and purchases data assets and verifies them by browsing the data directory information.Meanwhile,this paper designs a data structure Data Discrimination Bloom Filter(DDBF),making complaints about illegal data.When the number of data complaints reaches the threshold,the audit traceability contract is triggered to punish the illegal data publisher,aiming to improve the data quality and maintain a good data sharing ecology.In this paper,based on Ethereum,the above scheme is tested to demonstrate its feasibility,efficiency and security. 展开更多
关键词 Blockchain connected vehicles data sharing smart contracts credible traceability
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Functional connectivity of paired default mode network subregions in patients with eye trauma
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作者 Zheng-Mao Xing Dan Song +12 位作者 Jin-Yu Hu Xian-Mei Zhou Xuan Liao Cheng Chen Hong Wei Min Kang Qian Ling Liang-Qi He Zhen-Zhe Liu Jie Zou Xu Chen Zhen-Kai Wu Yi Shao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第12期2248-2255,共8页
AIM:To study functional brain abnormalities in patients with eye trauma(ET)and to discuss the pathophysiological mechanisms of ET.METHODS:Totally 31 ET patients and 31 healthy controls(HCs)were recruited.The age,gende... AIM:To study functional brain abnormalities in patients with eye trauma(ET)and to discuss the pathophysiological mechanisms of ET.METHODS:Totally 31 ET patients and 31 healthy controls(HCs)were recruited.The age,gender,and educational background characteristics of the two groups were similar.After functional magnetic resonance imaging(fMRI)scanning,the subjects’spontaneous brain activity was evaluated with the functional connectivity(FC)method.Receiver operating characteristic(ROC)curve analysis was used to classify the data.Pearson’s correlation analysis was used to explore the relationship between FC values in specific brain regions and clinical behaviors in patients with ET.RESULTS:Significantly increased FC between several regions was identified including the medial prefrontal cortex(MPFC)and left hippocampus formations(HF),the MPFC and left inferior parietal lobule(IPL),the left IPL and left medial temporal lobe(MTL),the left IPL and right MTL,and the right IPL and left MTL.No decreased region-to-region connectivity was detected in default mode network(DMN)sub-regions in patients with ET.Compared with HCs,ET patients exhibited significantly increased FC between several paired DMN regions,as follows:posterior cingulate cortex(PCC)and right HF(HF.R,t=2.196,P=0.032),right inferior parietal cortices(IPC.R)and left MTL(MTL.L,t=2.243,P=0.029),and right MTL(MTL.R)and HF.R(t=2.236,P=0.029).CONCLUSION:FC values in multiple brain regions of ET patients are abnormal,suggesting that these brain regions in ET patients may be dysfunctional,which may help to reveal the pathophysiological mechanisms of ET. 展开更多
关键词 eye trauma functional connectivity brain region
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Seismic fragility analysis of three-tower cable-stayed bridges with different connection configurations
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作者 Chen Chen Liu Jinlong +1 位作者 Lin Junqi Li Suchao 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第4期1009-1027,共19页
Seismic fragility analysis of three-tower cable-stayed bridges with three different structural systems,including rigid system(RS),floating system(FS),and passive energy dissipation system(PEDS),is conducted to study t... Seismic fragility analysis of three-tower cable-stayed bridges with three different structural systems,including rigid system(RS),floating system(FS),and passive energy dissipation system(PEDS),is conducted to study the effects of connection configurations on seismic responses and fragilities.Finite element models of bridges are established using OpenSees.A new ground motion screening method based on the statistical characteristic of the predominant period is proposed to avoid irregular behavior in the selection process of ground motions,and incremental dynamic analysis(IDA)is performed to develop components and systems fragility curves.The effects of damper failure on calculated results for PEDS are examined in terms of seismic response and fragility analysis.The results show that the bridge tower is the most affected component by different structural systems.For RS,the fragility of the middle tower is significantly higher than other components,and the bridge failure starts from the middle tower,exhibiting a characteristic of local failure.For FS and PEDS,the fragility of the edge tower is higher than the middle tower.The system fragility of RS is higher than FS and PEDS.Taking the failure of dampers into account is necessary to obtain reliable seismic capacity of cable-stayed bridges. 展开更多
关键词 seismic fragility cable-stayed bridge connection configuration viscous damper comparison analysis
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Two missense STK11 gene variations impaired LKB1/adenosine monophosphate-activated protein kinase signaling in Peutz-Jeghers syndrome
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作者 Jin Liu Si-Cong Zeng +3 位作者 An Wang Hai-Ying Cheng Qian-Jun Zhang Guang-Xiu Lu 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第4期1532-1546,共15页
BACKGROUND Peutz-Jeghers syndrome(PJS)is a rare hereditary neoplastic disorder mainly associated with serine/threonine kinase 11(STK11/LKB1)gene mutations.Preimplantation genetic testing can protect a patient’s offsp... BACKGROUND Peutz-Jeghers syndrome(PJS)is a rare hereditary neoplastic disorder mainly associated with serine/threonine kinase 11(STK11/LKB1)gene mutations.Preimplantation genetic testing can protect a patient’s offspring from mutated genes;however,some variations in this gene have been interpreted as variants of uncertain significance(VUS),which complicate reproductive decision-making in genetic counseling.AIM To identify the pathogenicity of two missense variants and provide clinical guidance.METHODS Whole exome gene sequencing and Sanger sequencing were performed on the peripheral blood of patients with PJS treated at the Reproductive and Genetic Hospital of Citic-Xiangya.Software was employed to predict the protein structure,conservation,and pathogenicity of the two missense variation sites in patients with PJS.Additionally,plasmids were constructed and transfected into HeLa cells to observe cell growth.The differences in signal pathway expression between the variant group and the wild-type group were compared using western blot and immunohistochemistry.Statistical analysis was performed using one-way analysis of variance.P<0.05 was considered statistically significant.RESULTS We identified two missense STK11 gene VUS[c.889A>G(p.Arg297Gly)and c.733C>T(p.Leu245Phe)]in 9 unrelated PJS families who were seeking reproductive assistance.The two missense VUS were located in the catalytic domain of serine/threonine kinase,which is a key structure of the liver kinase B1(LKB1)protein.In vitro experiments showed that the phosphorylation levels of adenosine monophosphate-activated protein kinase(AMPK)at Thr172 and LKB1 at Ser428 were significantly higher in transfected variation-type cells than in wild-type cells.In addition,the two missense STK11 variants promoted the proliferation of HeLa cells.Subsequent immunohistochemical analysis showed that phosphorylated-AMPK(Thr172)expression was significantly lower in gastric,colonic,and uterine polyps from PJS patients with missense variations than in non-PJS patients.Our findings indicate that these two missense STK11 variants are likely pathogenic and inactivate the STK11 gene,causing it to lose its function of regulating downstream phosphorylated-AMPK(Thr172),which may lead to the development of PJS.The identification of the pathogenic mutations in these two clinically characterized PJS patients has been helpful in guiding them toward the most appropriate mode of pregnancy assistance.CONCLUSION These two missense variants can be interpreted as likely pathogenic variants that mediated the onset of PJS in the two patients.These findings not only offer insights for clinical decision-making,but also serve as a foundation for further research and reanalysis of missense VUS in rare diseases. 展开更多
关键词 MISSENSE stk11 Peutz-Jeghers syndrome Rare disease Genetic counseling Assisted reproductive technique
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