目的建立骨骼肌胰岛素样生长因子1及胰岛素双受体功能缺失(Mouse overexpressing a dominantnegative IGF-I receptor specifically in muscle,MKR)转基因小鼠糖尿病合并心肌损伤的模型,观察MKR鼠在糖尿病病程中的心肌损伤表现。方法201...目的建立骨骼肌胰岛素样生长因子1及胰岛素双受体功能缺失(Mouse overexpressing a dominantnegative IGF-I receptor specifically in muscle,MKR)转基因小鼠糖尿病合并心肌损伤的模型,观察MKR鼠在糖尿病病程中的心肌损伤表现。方法2018年9月—2019年1月选取10只C57小鼠,设为正常组,普通饲料喂养;选取24只MKR鼠分为模型组、空白组,高脂饲料喂养,每组12只。模型组小鼠腹腔注射40mg/kg的链脲佐菌素(Streptozotocin,STZ,),正常组、空白组小鼠腹腔注射40mg/kg的柠檬酸盐缓冲液。记录各组小鼠一般情况、体质量及空腹血糖,检测TG、TC、LDL-C、HDL-C观察脂代谢情况,检测cTnI和CK-MB评估心肌损伤情况,光镜及电镜下观察心肌结构。结果第15周,模型组MKR鼠多食、多饮、多尿表现典型,体质量较第10周显著下降,差异有统计学意义(P<0.05)。模型组、空白组空腹血糖均≥11.1mmol/L,明显高于正常组小鼠,差异有统计学意义(P<0.05)。与空白组对比,模型组空腹血糖更高,差异有统计学意义(P<0.05)。与正常组比较,模型组TG、TC、LDL-C水平更高,HDL-C更低,差异有统计学意义(P<0.05)。与空白组相比,模型组MKR鼠cTnI、CK-MB水平更高,差异有统计学意义(P<0.05)。与空白组比较,模型组MKR鼠心肌组织在光镜及电镜下损伤程度更重。结论经高脂喂养联合STZ腹腔注射造模后的MKR鼠糖尿病特征典型,高糖、高脂显著,心肌损伤严重。展开更多
Objective:To investigate the hypoglycemic effect of the aqueous extract of Octomeles sumatrana (O.sumatrana)(OS) in streptozotocin-induced diabetic rats(STZ) and its molecular mechanisms. Methods:Diabetes was induced ...Objective:To investigate the hypoglycemic effect of the aqueous extract of Octomeles sumatrana (O.sumatrana)(OS) in streptozotocin-induced diabetic rats(STZ) and its molecular mechanisms. Methods:Diabetes was induced by intraperitoneal(i.p.) injection of streptozotocin(55 mg/kg) in to male Sprague-Dawley rats.Rats were divided into six different groups;normal control rats were not induced with STZ and served as reference,STZ diabetic control rats were given normal saline.Three groups were treated with OS aqueous extract at 0.2,0.3 and 0.5 g/kg,orally twice daily continuously for 21 d.The fifth group was treated with glibenclamide(6 mg/kg) in aqueous solution orally continuously for 21 d.After completion of the treatment period,biochemical parameters and expression levels of glucose transporter 2(Slc2a2),glucose-6-phosphatase (GoPase) and phosphoenolpyruvate carboxykinase(PCK1) were determined in liver by quantitative real time PCR.Results:Administration of OS at different doses to STZ induced diabetic rats, resulted in significant decrease(P【0.05) in blood glucose level in a dose dependent manner by 36%,48%,and 64%at doses of 0.2,0.3 and 0.5 g/kg,respectively,in comparison to the STZ control values.Treatment with OS elicited an increase in the expression level of Slc2a2 gene but reduced the expression of G6Pase and PCK1 genes.Morefore,OS treated rats,showed significantly lower levels of serum alanine transaminase(ALT),aspartate aminotransferase(AST) and urea levels compared to STZ untreated rats.The extract at different doses elicited signs of recovery in body weight gain when compared to STZ diabetic controls although food and water consumption were significantly lower in treated groups compared to STZ diabetic control group.Conclusions:O.sumatrana aqueous extract is beneficial for improvement of hyperglycemia by increasing gene expression of liver Slc2a2 and reducing expression of G6Pase and PCK1 genes in streptozotocin-induced diabetic rats.展开更多
文摘目的建立骨骼肌胰岛素样生长因子1及胰岛素双受体功能缺失(Mouse overexpressing a dominantnegative IGF-I receptor specifically in muscle,MKR)转基因小鼠糖尿病合并心肌损伤的模型,观察MKR鼠在糖尿病病程中的心肌损伤表现。方法2018年9月—2019年1月选取10只C57小鼠,设为正常组,普通饲料喂养;选取24只MKR鼠分为模型组、空白组,高脂饲料喂养,每组12只。模型组小鼠腹腔注射40mg/kg的链脲佐菌素(Streptozotocin,STZ,),正常组、空白组小鼠腹腔注射40mg/kg的柠檬酸盐缓冲液。记录各组小鼠一般情况、体质量及空腹血糖,检测TG、TC、LDL-C、HDL-C观察脂代谢情况,检测cTnI和CK-MB评估心肌损伤情况,光镜及电镜下观察心肌结构。结果第15周,模型组MKR鼠多食、多饮、多尿表现典型,体质量较第10周显著下降,差异有统计学意义(P<0.05)。模型组、空白组空腹血糖均≥11.1mmol/L,明显高于正常组小鼠,差异有统计学意义(P<0.05)。与空白组对比,模型组空腹血糖更高,差异有统计学意义(P<0.05)。与正常组比较,模型组TG、TC、LDL-C水平更高,HDL-C更低,差异有统计学意义(P<0.05)。与空白组相比,模型组MKR鼠cTnI、CK-MB水平更高,差异有统计学意义(P<0.05)。与空白组比较,模型组MKR鼠心肌组织在光镜及电镜下损伤程度更重。结论经高脂喂养联合STZ腹腔注射造模后的MKR鼠糖尿病特征典型,高糖、高脂显著,心肌损伤严重。
文摘Objective:To investigate the hypoglycemic effect of the aqueous extract of Octomeles sumatrana (O.sumatrana)(OS) in streptozotocin-induced diabetic rats(STZ) and its molecular mechanisms. Methods:Diabetes was induced by intraperitoneal(i.p.) injection of streptozotocin(55 mg/kg) in to male Sprague-Dawley rats.Rats were divided into six different groups;normal control rats were not induced with STZ and served as reference,STZ diabetic control rats were given normal saline.Three groups were treated with OS aqueous extract at 0.2,0.3 and 0.5 g/kg,orally twice daily continuously for 21 d.The fifth group was treated with glibenclamide(6 mg/kg) in aqueous solution orally continuously for 21 d.After completion of the treatment period,biochemical parameters and expression levels of glucose transporter 2(Slc2a2),glucose-6-phosphatase (GoPase) and phosphoenolpyruvate carboxykinase(PCK1) were determined in liver by quantitative real time PCR.Results:Administration of OS at different doses to STZ induced diabetic rats, resulted in significant decrease(P【0.05) in blood glucose level in a dose dependent manner by 36%,48%,and 64%at doses of 0.2,0.3 and 0.5 g/kg,respectively,in comparison to the STZ control values.Treatment with OS elicited an increase in the expression level of Slc2a2 gene but reduced the expression of G6Pase and PCK1 genes.Morefore,OS treated rats,showed significantly lower levels of serum alanine transaminase(ALT),aspartate aminotransferase(AST) and urea levels compared to STZ untreated rats.The extract at different doses elicited signs of recovery in body weight gain when compared to STZ diabetic controls although food and water consumption were significantly lower in treated groups compared to STZ diabetic control group.Conclusions:O.sumatrana aqueous extract is beneficial for improvement of hyperglycemia by increasing gene expression of liver Slc2a2 and reducing expression of G6Pase and PCK1 genes in streptozotocin-induced diabetic rats.
