For gene transfer in fish, the cellular or viral promoters derived from mammalian are probably the only choice to use for the time being. This is due to the fact that the promoter of homologous transcriptional regulat...For gene transfer in fish, the cellular or viral promoters derived from mammalian are probably the only choice to use for the time being. This is due to the fact that the promoter of homologous transcriptional regulatory sequences is absent in fish.In this study, the bacterial gene for chloramphenicol acetyltransferase (CAT) under the control of the early promoter from simian virus 40 (SV40)was used to transfect the cultured kidney cells of grass carp and efficient expression was obtained. It is suggested that the mammalian viral SV40 promoter can be used for the expression of the foreign gene in transgenic fish.展开更多
文摘For gene transfer in fish, the cellular or viral promoters derived from mammalian are probably the only choice to use for the time being. This is due to the fact that the promoter of homologous transcriptional regulatory sequences is absent in fish.In this study, the bacterial gene for chloramphenicol acetyltransferase (CAT) under the control of the early promoter from simian virus 40 (SV40)was used to transfect the cultured kidney cells of grass carp and efficient expression was obtained. It is suggested that the mammalian viral SV40 promoter can be used for the expression of the foreign gene in transgenic fish.
