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大肠埃希氏菌噬菌体swi2裂解系统的表达及协同抑菌活性测定
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作者 邹玲 赵天祎 +3 位作者 王馨锐 甄建瑜 刘文华 张灿 《动物医学进展》 北大核心 2024年第4期75-81,共7页
为了确定大肠埃希氏菌噬菌体swi2裂解系统的穿孔素(holin)与裂解酶(lysin1、lysin2)在裂解宿主菌过程中的协同作用,根据噬菌体swi2裂解系统的基因序列设计引物,搭桥PCR法分别扩增holin-lysin1、holin-lysin2、lysin1-lysin2基因,以pCol... 为了确定大肠埃希氏菌噬菌体swi2裂解系统的穿孔素(holin)与裂解酶(lysin1、lysin2)在裂解宿主菌过程中的协同作用,根据噬菌体swi2裂解系统的基因序列设计引物,搭桥PCR法分别扩增holin-lysin1、holin-lysin2、lysin1-lysin2基因,以pColdTF为载体,构建重组质粒pCold-holin-lysin1、pCold-holin-lysin2、pCold-lysin1-lysin2,在大肠埃希氏菌BL21中诱导表达,并测定各串联蛋白对大肠埃希氏菌BL21的菌内外裂解活性。结果表明,成功构建了噬菌体swi2裂解系统相关基因两两串联的重组质粒,并在原核系统中实现了holin-lysin1、holin-lysin2、lysin1-lysin2蛋白的可溶性表达。lysin1、lysin2具有天然的菌内和菌外裂解活性,而单独的holin未表现出任何抑菌活性;lysin1与lysin2、holin与lysin1之间无协同作用,而holin与lysin2之间存在协同作用,可以显著增强lysin2的菌内外裂解活性(P<0.05)。研究结果证明了lysin1和lysin2在噬菌体swi2增殖后期替代holin完成裂解宿主菌的过程,两者之间不存在协同作用,这将为进一步阐明噬菌体swi2的裂解机制提供参考。 展开更多
关键词 噬菌体swi2 穿孔素 裂解酶 协同作用 抑菌活性
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Stochastic Simulation of Saline Intrusion in the Coastal Aquifer of Saloum, Senegal
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作者 Seyni Ndoye Amadou Sarr +3 位作者 Mathieu Le Coz Cheikh Becaye Gaye Moumtaz Razack Philippe Le Coustumer 《Journal of Environmental Protection》 2024年第8期863-873,共11页
In the Saloum region of central-western Senegal, water needs are essentially met by tapping an underground aquifer associated with the sandy-clay formations of the Continental Terminal, in contact with both the ocean ... In the Saloum region of central-western Senegal, water needs are essentially met by tapping an underground aquifer associated with the sandy-clay formations of the Continental Terminal, in contact with both the ocean to the west and the highly saline waters of the Saloum River to the north. In this estuarine and deltaic zone with its very low relief, the hydraulic loads in the water tables are generally close to zero or even negative, creating a reversal of the natural flow and encouraging saline intrusion into this system, which makes it very vulnerable. This study concerns the implementation of a numerical model of saline intrusion to provide a better understanding of the vulnerability of the water table by analyzing the variability of the freshwater/saltwater interface. The Modflow-2005 code is used to simulate saline intrusion using the SWI2 module, coupled with the GRASS (Geographic Resources Analysis Support System) software under the Linux operating system with the steep interface approach. The probable expansion of the wedge is studied in three scenarios, taking into account its position relative to the bedrock at 1 m, 5 m and 10 m. Simulations carried out under imposed potential and river conditions, based on variations in groundwater reserves using two effective porosity values, 10−1 and 10−2, show that the water table is highly vulnerable in the northwest sector. The probable expansion of the wedge increases as the storage coefficient decreases and is more marked with river conditions in the areas surrounding the Saloum River, reaching 6 km with a probability of 1. The probability of the wedge reaching a certain degree of expansion decreases from 1 to 0.5, and then cancels out as it moves inland. The probable position of the wedge is limited to 500 m or even 1 km depending on the corner around the coast to the southwest and in the southern zone. This modelling, carried out under natural conditions, will be developed further, taking into account climatic parameters and pumping from wells and boreholes. 展开更多
关键词 Saline Intrusion Stochastic Modelling MODFLOW swi2 Grass GIS AQUIFER Saloum Senegal
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染色质重构因子CHD蛋白家族的研究进展 被引量:3
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作者 王会平 徐勤枝 周平坤 《国际放射医学核医学杂志》 2006年第1期42-47,共6页
染色质重构是DNA修复、基因表达调控过程中的一个重要环节。染色质重构使染色质组织结构发生一系列重要的变化,如染色质去凝集,核小体变成开放式的疏松结构,使转录因子等更易接近并结合核小体DNA,从而调控基因转录等。CHD蛋白是目前已... 染色质重构是DNA修复、基因表达调控过程中的一个重要环节。染色质重构使染色质组织结构发生一系列重要的变化,如染色质去凝集,核小体变成开放式的疏松结构,使转录因子等更易接近并结合核小体DNA,从而调控基因转录等。CHD蛋白是目前已知的染色质重构复合物之一。目前已鉴定了6个人类CHD蛋白成员,主要有3种功能结构域:N端的两个染色质调节域,位于中部的类SWI2/SNF2 ATP酶/解旋酶域,以及C端的DNA结合域。CHD基因突变或表达异常与人类某些疾病有关。 展开更多
关键词 染色质重构 ATP酶 CHD swi2/SNF2
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