Objective: To study the enhancement of the immune functions and autologous tumor killing (ATK) activity by kappa selenocarrageenan (KSC) in mice bearing sarcoma 180. Methods: To measure the effects of KSC and/or Cy...Objective: To study the enhancement of the immune functions and autologous tumor killing (ATK) activity by kappa selenocarrageenan (KSC) in mice bearing sarcoma 180. Methods: To measure the effects of KSC and/or Cyclophosphamide (Cy) on natural killer (NK) activity, lymphokine activated killer (LAK) activity, the produc tion of interleukin 2 (IL 2), ATK activity and the growth of sarcoma 180 (S 180 ). Results: KSC promoted NK activity, LAK activity and ATK activity in vivo , increased IL 2 production at 40 mg/kg/d×9d. It also enhanced the antitumor action of Cy (20 mg/kg/d×9d) and offset the inhibition of Cy on immunocopetent cells. The ATK activity in splenocytes of S 180 bearing mice could be induced and increased by recombinant interleukin 2 (rIL 2) in vitro . Conclusion: KSC has an up regulating effect on the immune functions and ATK activity in tumor bearing mice. It can be used as a biological response modifier (BRM) in cancer biotherapy.展开更多
Kunming female mice were used and S180 sarcoma cells were transplanted subeutaneously into right anterior armpit or the mice. The mice were divided into three groups randomly, and orally administered with water, galli...Kunming female mice were used and S180 sarcoma cells were transplanted subeutaneously into right anterior armpit or the mice. The mice were divided into three groups randomly, and orally administered with water, gallium chloride and GaCl3+ Na2SeO3 respectively for 2 weeks. After that,the tumors and foreleg bones were taken out for study. The experimental results showed that oral administration with gallium chloride-could inhibit the growth or S180 sarcoma in mice and the tumor morphology was changed. Selenium could accelerate the gallium entering into bone. This fact might supply a new clue to further clinical application of gallium and selenium.展开更多
Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Method...Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.展开更多
The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were...The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were studied through electron microscope in this article. The results indicated that the ultrastructures of.S-180 sarcoma cells were destructed obviously by the administratiouof GP to the tumor-bearing mouse with a Pattern or dose-depeudant, especially to the cellular nuclear. We saw both apoptosis and necrosis in morphologic alterations in tumor cells, suck as a reductionin cellular volume. an increase in cytoplasm, uucleoplasm electron density and condensation of nuclear chromatiu either to periphery or the nuclear membrane or inclumps within the cell, lots of inthe cytoplasm and apoptotic body in some turner cells or some cytoplast breaking into small frag- ments etc.展开更多
文摘Objective: To study the enhancement of the immune functions and autologous tumor killing (ATK) activity by kappa selenocarrageenan (KSC) in mice bearing sarcoma 180. Methods: To measure the effects of KSC and/or Cyclophosphamide (Cy) on natural killer (NK) activity, lymphokine activated killer (LAK) activity, the produc tion of interleukin 2 (IL 2), ATK activity and the growth of sarcoma 180 (S 180 ). Results: KSC promoted NK activity, LAK activity and ATK activity in vivo , increased IL 2 production at 40 mg/kg/d×9d. It also enhanced the antitumor action of Cy (20 mg/kg/d×9d) and offset the inhibition of Cy on immunocopetent cells. The ATK activity in splenocytes of S 180 bearing mice could be induced and increased by recombinant interleukin 2 (rIL 2) in vitro . Conclusion: KSC has an up regulating effect on the immune functions and ATK activity in tumor bearing mice. It can be used as a biological response modifier (BRM) in cancer biotherapy.
文摘Kunming female mice were used and S180 sarcoma cells were transplanted subeutaneously into right anterior armpit or the mice. The mice were divided into three groups randomly, and orally administered with water, gallium chloride and GaCl3+ Na2SeO3 respectively for 2 weeks. After that,the tumors and foreleg bones were taken out for study. The experimental results showed that oral administration with gallium chloride-could inhibit the growth or S180 sarcoma in mice and the tumor morphology was changed. Selenium could accelerate the gallium entering into bone. This fact might supply a new clue to further clinical application of gallium and selenium.
基金supported by grants from FAPES(Fundacao de Amparo a Pesquisa e Inovacao do Espirito Santo)-term of grant 225/2015
文摘Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.
文摘The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were studied through electron microscope in this article. The results indicated that the ultrastructures of.S-180 sarcoma cells were destructed obviously by the administratiouof GP to the tumor-bearing mouse with a Pattern or dose-depeudant, especially to the cellular nuclear. We saw both apoptosis and necrosis in morphologic alterations in tumor cells, suck as a reductionin cellular volume. an increase in cytoplasm, uucleoplasm electron density and condensation of nuclear chromatiu either to periphery or the nuclear membrane or inclumps within the cell, lots of inthe cytoplasm and apoptotic body in some turner cells or some cytoplast breaking into small frag- ments etc.
