[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangs...[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.[Result] Hormone combination 0.10 mg/L 6-BA ＋0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA ＋0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.[Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia.展开更多
[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extrac...[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.展开更多
用自然群体取样和同工酶遗传标记的方法对沼生植物野慈姑（Sagittaria trifolia Linn.）及其变型长瓣慈姑[S.trifolia Linn. f. longiloba (Turcz.) Makino]的异交率作了定量研究，3个自然群体的异交率估计值为=91.0％～98.0％，表明该...用自然群体取样和同工酶遗传标记的方法对沼生植物野慈姑（Sagittaria trifolia Linn.）及其变型长瓣慈姑[S.trifolia Linn. f. longiloba (Turcz.) Makino]的异交率作了定量研究，3个自然群体的异交率估计值为=91.0％～98.0％，表明该种为异交占绝对优势的交配系统。在野慈姑各群体间未发现异交率的显著差异。展开更多
基金Supported by Science and Technology Foundation of Guizhou Province(20092108)Key Agricultural Program from Science and Technology Bureau of Guiyang City(201001)Science Research Program for Doctors in Guizhou Normal University(2008)~~
文摘[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.[Result] Hormone combination 0.10 mg/L 6-BA ＋0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA ＋0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.[Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia.
基金Supported by Scientific Research Project of Education Department of Sichuan Province(16ZB0199)~~
文摘[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.
文摘用自然群体取样和同工酶遗传标记的方法对沼生植物野慈姑（Sagittaria trifolia Linn.）及其变型长瓣慈姑[S.trifolia Linn. f. longiloba (Turcz.) Makino]的异交率作了定量研究，3个自然群体的异交率估计值为=91.0％～98.0％，表明该种为异交占绝对优势的交配系统。在野慈姑各群体间未发现异交率的显著差异。
