Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the sal...Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.展开更多
The vacuolar proton pump ATPase(V-H^+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several ...The vacuolar proton pump ATPase(V-H^+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H^+-ATPase subunits B(Sc VHA-B, Gen Bank: JF826506), C(Sc VHA-C, Gen Bank: JF826507) and H(Sc VHA-H, Gen Bank: JF826508) were isolated from the halophyte Suaeda corniculata. The transcript levels of Sc VHA-B, Sc VHA-C and Sc VHA-H were increased by salt, drought and saline-alkali treatments. V-H^+-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H^+-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H^+-ATPase subunits B, C and H(Sc VHA-B, Sc VHA-C and Sc VHA-H) from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type(WT). More free proline, higher superoxide dismutase(SOD) activity and lower malondialdehyde(MDA) levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the Sc VHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of Sc VHA-B, Sc VHA-C and Sc VHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.展开更多
The communication complexity of the practical byzantine fault tolerance(PBFT)protocol is reduced with the threshold signature technique applied to the consensus process by phase voting PBFT(PV-PBFT).As most communicat...The communication complexity of the practical byzantine fault tolerance(PBFT)protocol is reduced with the threshold signature technique applied to the consensus process by phase voting PBFT(PV-PBFT).As most communication occurs between the primary node and replica nodes in PV-PVFT,consistency verification is accomplished through threshold signatures,multi-PV,and multiple consensus.The view replacement protocol introduces node weights to influence the election of a primary node,reducing the probability of the same node being elected primary multiple times.The experimental results of consensus algorithms show that compared to PBFT,the communication overhead of PV-PBFT decreases by approximately 90% with nearly one-time improvement in the throughput relative and approximately 2/3 consensus latency,lower than that of the scalable hierarchical byzantine fault tolerance.The communication complexity of the PBFT is O(N^(2)),whereas that of PV-PBFT is only O(N),which implies the significant improvement of the operational efficiency of the blockchain system.展开更多
The threshold cryptography provides a new approach to building intrusion tolerance applications. In this paper, a threshold decryption scheme based elliptic curve cryptography is presented. A zero-knowledge test appro...The threshold cryptography provides a new approach to building intrusion tolerance applications. In this paper, a threshold decryption scheme based elliptic curve cryptography is presented. A zero-knowledge test approach based on elliptic curve cryptography is designed. The application of these techniques in Web security is studied. Performance analysis shows that our scheme is characterized by excellent security as well as high efficiency.展开更多
This paper presents a novel technique for improved voting by adaptively varying the membership boundaries of a fuzzy voter to achieve realistic consensus among inputs of redundant modules of a fault tolerant system. W...This paper presents a novel technique for improved voting by adaptively varying the membership boundaries of a fuzzy voter to achieve realistic consensus among inputs of redundant modules of a fault tolerant system. We demonstrate that suggested dynamic membership partitioning minimizes the number of occurrences of incorrect outputs of a voter as compared to the fixed membership partitioning voter implementations. Simulation results for the proposed voter for Triple Modular Redundancy (TMR) fault tolerant system indicate that our algorithm shows better safety and availability performance as compared to the existing one. However, our voter design is general and thus it can be potentially useful for improving safety and availability of critical fault tolerant systems.展开更多
针对联盟链微电网电力交易场景的高吞吐量、高数据安全性及数据透明性的需求,提出一种在贡献值模型下的基于可验证随机函数(Verifiable Random Function,VRF)与基于BLS(Boneh-Lynn-Shacham Threshold Signatures,BLS)门限签名的改进实...针对联盟链微电网电力交易场景的高吞吐量、高数据安全性及数据透明性的需求,提出一种在贡献值模型下的基于可验证随机函数(Verifiable Random Function,VRF)与基于BLS(Boneh-Lynn-Shacham Threshold Signatures,BLS)门限签名的改进实用拜占庭容错共识算法(Contribution Value Model,Verifiable Random Function and Boneh-Lynn-Shacham Threshold Signatures Practical ByzantineFault Tolerance,CVB-PBFT)。CVB-PBFT算法通过贡献值模型筛选高贡献节点参与共识,采用VRF和安全随机函数选举不可预测的主节点,结合节点轮换和检测机制以及BLS签名优化通信流程,显著提高算法的性能和安全性。经实验证明,该算法能够有效防御恶意攻击,降低通信开销,并提升共识效率,满足微电网电力交易对时效性和安全性的要求。展开更多
Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of...Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of 80 adult Wistar rats were randomly selected to establish chronic MPS rat models,and randomly divided into four groups:control group,acupuncture group,internal heat acupuncture group A,and internal heat acupuncture group B,with 20 rats in each group.The rats in the control group were not given any treatment,and the rats in the acupuncture group were only given acupuncture treatment.The rats in group A were treated with internal heat acupuncture(needle heating up to 42℃),and the rats in group B were treated with internal heat acupuncture(needle heating up to 44℃).The tolerance time of plantar hot plate,the pain threshold of gastrocnemius muscle and the level of tumor necrosis factor-α(TNF-α)were observed and compared before modeling,1 d before treatment,and 1,7 and 14 d after treatment.Results:There were significant differences in hot plate tolerance time,time and interaction among the four groups(P<0.05),as well as in gastrocnemius tenderness threshold,time and interaction among the four groups(P<0.05),and there were also significant differences in TNF-αlevel among the three groups(P>0.05).There were significant differences in the number of electric shocks,time and interaction among the four groups(P<0.05).Conclusion:In contrast to conventional acupuncture treatment,internal heat acupuncture demonstrates greater efficacy in extending the tolerance duration of hot plate exposure and enhancing the pain threshold of the gastrocnemius muscle in rats afflicted with myofascial pain syndrome.Additionally,it accelerates the amelioration of inflammatory markers and motor function.However,it is important to note that the therapeutic impact of internal heat acupuncture may be influenced by its temperature,with 44℃being the most effective in this research.展开更多
基金funded by the Key-Area Research and Development Program of Guangdong Province,China(Grant No.2020B020219004)the Hainan Yazhou Bay Seed Lab(Grant No.B21HJ0216)the Agricultural Science and Technology Innovation Program and the Cooperation and Innovation Mission,China(Grant No.CAAS-ZDXT202001)。
文摘Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.
基金supported by the National Natural Science Foundation of China (31271746, 31401403, 31501366,31201237)
文摘The vacuolar proton pump ATPase(V-H^+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H^+-ATPase subunits B(Sc VHA-B, Gen Bank: JF826506), C(Sc VHA-C, Gen Bank: JF826507) and H(Sc VHA-H, Gen Bank: JF826508) were isolated from the halophyte Suaeda corniculata. The transcript levels of Sc VHA-B, Sc VHA-C and Sc VHA-H were increased by salt, drought and saline-alkali treatments. V-H^+-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H^+-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H^+-ATPase subunits B, C and H(Sc VHA-B, Sc VHA-C and Sc VHA-H) from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type(WT). More free proline, higher superoxide dismutase(SOD) activity and lower malondialdehyde(MDA) levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the Sc VHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of Sc VHA-B, Sc VHA-C and Sc VHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.
基金The National Key R&D Program of China(No.2020YFE0200600)。
文摘The communication complexity of the practical byzantine fault tolerance(PBFT)protocol is reduced with the threshold signature technique applied to the consensus process by phase voting PBFT(PV-PBFT).As most communication occurs between the primary node and replica nodes in PV-PVFT,consistency verification is accomplished through threshold signatures,multi-PV,and multiple consensus.The view replacement protocol introduces node weights to influence the election of a primary node,reducing the probability of the same node being elected primary multiple times.The experimental results of consensus algorithms show that compared to PBFT,the communication overhead of PV-PBFT decreases by approximately 90% with nearly one-time improvement in the throughput relative and approximately 2/3 consensus latency,lower than that of the scalable hierarchical byzantine fault tolerance.The communication complexity of the PBFT is O(N^(2)),whereas that of PV-PBFT is only O(N),which implies the significant improvement of the operational efficiency of the blockchain system.
基金Supported by the Foundation of National 863 Programme of China (No. 2002AA142040)
文摘The threshold cryptography provides a new approach to building intrusion tolerance applications. In this paper, a threshold decryption scheme based elliptic curve cryptography is presented. A zero-knowledge test approach based on elliptic curve cryptography is designed. The application of these techniques in Web security is studied. Performance analysis shows that our scheme is characterized by excellent security as well as high efficiency.
文摘This paper presents a novel technique for improved voting by adaptively varying the membership boundaries of a fuzzy voter to achieve realistic consensus among inputs of redundant modules of a fault tolerant system. We demonstrate that suggested dynamic membership partitioning minimizes the number of occurrences of incorrect outputs of a voter as compared to the fixed membership partitioning voter implementations. Simulation results for the proposed voter for Triple Modular Redundancy (TMR) fault tolerant system indicate that our algorithm shows better safety and availability performance as compared to the existing one. However, our voter design is general and thus it can be potentially useful for improving safety and availability of critical fault tolerant systems.
文摘针对联盟链微电网电力交易场景的高吞吐量、高数据安全性及数据透明性的需求,提出一种在贡献值模型下的基于可验证随机函数(Verifiable Random Function,VRF)与基于BLS(Boneh-Lynn-Shacham Threshold Signatures,BLS)门限签名的改进实用拜占庭容错共识算法(Contribution Value Model,Verifiable Random Function and Boneh-Lynn-Shacham Threshold Signatures Practical ByzantineFault Tolerance,CVB-PBFT)。CVB-PBFT算法通过贡献值模型筛选高贡献节点参与共识,采用VRF和安全随机函数选举不可预测的主节点,结合节点轮换和检测机制以及BLS签名优化通信流程,显著提高算法的性能和安全性。经实验证明,该算法能够有效防御恶意攻击,降低通信开销,并提升共识效率,满足微电网电力交易对时效性和安全性的要求。
文摘Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of 80 adult Wistar rats were randomly selected to establish chronic MPS rat models,and randomly divided into four groups:control group,acupuncture group,internal heat acupuncture group A,and internal heat acupuncture group B,with 20 rats in each group.The rats in the control group were not given any treatment,and the rats in the acupuncture group were only given acupuncture treatment.The rats in group A were treated with internal heat acupuncture(needle heating up to 42℃),and the rats in group B were treated with internal heat acupuncture(needle heating up to 44℃).The tolerance time of plantar hot plate,the pain threshold of gastrocnemius muscle and the level of tumor necrosis factor-α(TNF-α)were observed and compared before modeling,1 d before treatment,and 1,7 and 14 d after treatment.Results:There were significant differences in hot plate tolerance time,time and interaction among the four groups(P<0.05),as well as in gastrocnemius tenderness threshold,time and interaction among the four groups(P<0.05),and there were also significant differences in TNF-αlevel among the three groups(P>0.05).There were significant differences in the number of electric shocks,time and interaction among the four groups(P<0.05).Conclusion:In contrast to conventional acupuncture treatment,internal heat acupuncture demonstrates greater efficacy in extending the tolerance duration of hot plate exposure and enhancing the pain threshold of the gastrocnemius muscle in rats afflicted with myofascial pain syndrome.Additionally,it accelerates the amelioration of inflammatory markers and motor function.However,it is important to note that the therapeutic impact of internal heat acupuncture may be influenced by its temperature,with 44℃being the most effective in this research.