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转录组与代谢组联合分析三蕊柳对柳蓝叶甲的抗虫机制
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作者 王书柔 闫晓丽 +1 位作者 刘兴菊 梁海永 《林业与生态科学》 2024年第4期388-397,共10页
为研究三蕊柳抗柳蓝叶甲的机制及其中的原理,以垂柳、黄花柳、旱柳为代表的非抗虫组和以3个无性系的三蕊柳为代表的抗虫组4种不同且相关的柳树品种为试验对象,以差异代谢物儿茶素溶液、水杨酸溶液、没食子酰葡萄糖溶液、新橙皮苷溶液以... 为研究三蕊柳抗柳蓝叶甲的机制及其中的原理,以垂柳、黄花柳、旱柳为代表的非抗虫组和以3个无性系的三蕊柳为代表的抗虫组4种不同且相关的柳树品种为试验对象,以差异代谢物儿茶素溶液、水杨酸溶液、没食子酰葡萄糖溶液、新橙皮苷溶液以及异泽兰黄素溶液浸泡处理的叶片饲喂柳蓝叶甲成虫及幼虫进行试验,探究三蕊柳抗柳蓝叶甲的机制。结果表明:(1)柳蓝叶甲在食用异泽兰黄素溶液处理的叶片后出现死亡:24 h后成虫存活率平均为83.33%,48 h后成虫存活率平均为40%;24 h后幼虫存活率平均为80%,48 h后幼虫存活率平均为35%。(2)其他溶液处理的叶片均未造成柳蓝叶甲死亡:24 h后及48 h后,成虫、幼虫的成活率均为100%。(3)结合转录组与代谢组分析结果筛选出来的5种差异代谢物溶液处理叶片饲喂柳蓝叶甲之后,只有异泽兰黄素溶液处理出现柳蓝叶甲死亡,异泽兰黄素可能是三蕊柳抗柳蓝叶甲的重要原因。综上所述,推测三蕊柳抗虫机制与次生代谢物——异泽兰黄素有关。 展开更多
关键词 三蕊柳 柳蓝叶甲 异泽兰黄素 次生代谢物
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Leaf litter ecological fate in the Schelde Estuary in Belgium 被引量:2
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作者 LUOYi MickyTackx +2 位作者 LIFa-yun MAODa-qing ZHOUQi-xing 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2002年第4期563-567,共5页
Two dominant species of Willow( Salix triandra )and Reed (Phragmites australis) along the Schelde Estuary(in Belgium)were selected in this research. The pigments of higher plant was used as biomarkers, the deco... Two dominant species of Willow( Salix triandra )and Reed (Phragmites australis) along the Schelde Estuary(in Belgium)were selected in this research. The pigments of higher plant was used as biomarkers, the decomposition process of the two species were studied after they fall into the Schelde Estuary. After statistical analysis(Spearman rank order correlation, P <0 05), the results has shown the decomposition dynamics pattern of the pigments, and the willow showed different pattern in comparing with the reed, e.g. Chlorophyll a decomposition dynamics for willow is: y 1=12196 x 2 - 175895 x +1E+06+ k , R 2=0 5706 while for reed is: y 2=-37878 x 2+229782 x +734282+ k , R 2=0 9065 The precise time of the leaf litter spent in the water was also calculated as were less than 24 days, 24-37 days, longer than 37 days(willow)and less than 24 days, longer than 24 days(reed), the leaf litter fate of the two dominant species in the Schelde Estuary was also compared. 展开更多
关键词 leaf litter ecologcal fate salix triandra Phragmites australis Schelde Estuary
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Decomposition dynamic of higher plant pigments by HPLC analysis
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作者 LUOYi ZHANGTing-zhou +2 位作者 ZHOUQi-xing MAODa-qing WANDong-mei 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2004年第5期847-850,共4页
The fate of the litter of dominant vegetation(willows and reeds) is one of the aspects studied in the frame of the project “Onderzoek Milieu Effecten Sigmaplan”. One of the questions to be considered is how long the... The fate of the litter of dominant vegetation(willows and reeds) is one of the aspects studied in the frame of the project “Onderzoek Milieu Effecten Sigmaplan”. One of the questions to be considered is how long the litter stays within the estuary. In this paper, the time the leaf litter(Salix triandra and Phragmites australis) stayed in the Schelde estuary was studied by using plant pigment as biomarkers with HPLC application. After analyzing the original data from the incubation experiment described by Dubuison and Geers(1999), the decomposition dynamics patterns of pigments were analyzed and described, and these decomposition dynamics patterns were used as calibration patterns. By using Spearman Rank Order Correlation, the calibration patterns of the pigments which were significant(p<0.05) were grouped. In this way, several groups of the calibration patterns of pigment decomposition were achieved. The presence or absence of these groups of pigments (whether they can be detected or not from HPLC) was shown to be useful in determining the time the litter has stayed in the water. Combining data of DW and POC, more precise timing can be obtained. 展开更多
关键词 decomposition dynamics pattern salix triandra Phragmites australis Spearman Rank Order Correlation Schelde Estuary HPLC
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三蕊柳的组织培养及快速繁殖的研究 被引量:3
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作者 张云慧 姜长阳 《哈尔滨师范大学自然科学学报》 CAS 2011年第3期76-79,共4页
以三蕊柳的休眠芽为材料,进行了萌动芽的生长,不定芽的分化,试管苗的继代培养与留茬培养,生根苗扦插和移植的研究,成功地建立起三蕊柳快速繁殖技术.研究结果证明:MS+BA0.1 mg.L-1+GA 1.0 mg.L-1+IAA 0.2 mg.L-1是休眠芽生长培养的理想... 以三蕊柳的休眠芽为材料,进行了萌动芽的生长,不定芽的分化,试管苗的继代培养与留茬培养,生根苗扦插和移植的研究,成功地建立起三蕊柳快速繁殖技术.研究结果证明:MS+BA0.1 mg.L-1+GA 1.0 mg.L-1+IAA 0.2 mg.L-1是休眠芽生长培养的理想培养基;1/2MS+BA 0.6 mg.L-1+IAA 0.2 mg.L-1+NAA 0.1mg.L-1是生长芽分化培养的理想培养基;1/2MS+BA 0.4mg.L-1+AgNO30.8 mg.L-1+GA30.5 mg.L-1+IAA 0.3 mg.L-1+NAA 0.1 mg.L-1是不定芽分化继代培养和留茬继代培养的理想培养基;移植到河滩上的试管苗生长旺盛,呈丛生状. 展开更多
关键词 三蕊柳 组织培养 快速繁殖
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6种柳树对柳蓝叶甲的抗性鉴定及主要代谢物分析
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作者 李文杰 崔露露 +3 位作者 麻芸娇 王春荣 郭娇娇 梁海永 《林业与生态科学》 2021年第4期379-385,共7页
为了探究不同柳树对柳蓝叶甲的抗性表现,筛选出具有优良抗性的品种,以三蕊柳、蒿柳、红皮柳、黄花柳、垂柳、白柳6种柳树为试验材料,进行了田间调查和室内饲虫试验。结果表明,在田间自然条件下三蕊柳的叶片未受侵害,成虫数量及幼虫数量... 为了探究不同柳树对柳蓝叶甲的抗性表现,筛选出具有优良抗性的品种,以三蕊柳、蒿柳、红皮柳、黄花柳、垂柳、白柳6种柳树为试验材料,进行了田间调查和室内饲虫试验。结果表明,在田间自然条件下三蕊柳的叶片未受侵害,成虫数量及幼虫数量均为零,表现出了高抗。在室内饲虫试验中,柳蓝叶甲成虫在饲养前5 d未取食三蕊柳,5 d后开始出现取食行为,但叶片取食面积显著低于其他品种。饲养15 d后成虫的存活率仅为6.67%,显著低于喂食其他品种的存活率。柳蓝叶甲幼虫在饲养前3 d内对三蕊柳叶片的取食量显著低于喂食其他品种的,且在喂食4 d后全部死亡。根据抗虫性鉴定试验证明三蕊柳为柳蓝叶甲高抗品种。测定三蕊柳与其他5种柳树的差异次生代谢产物得出异泽兰黄素-葡萄糖苷、异泽兰黄素、2a羟基-齐墩果酸、木犀草素-香豆酰辅酶A己糖、2-O-二没食子酰基-β-D-吡喃葡萄糖苷-β-D-吡喃葡萄糖苷、6,8-二异戊烯基香豌豆苷元6种主要的上调差异次生代谢产物。 展开更多
关键词 柳树 柳蓝叶甲 三蕊柳 抗虫性 次生代谢物
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