Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance （SPR） biosensor wa...Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance （SPR） biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically ＂taste＂ the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif- ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con- centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 x 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.展开更多
由于沙门氏菌引起的食物中毒不仅危害人们的身体健康,而且还会造成严重的经济损失。随着人们对食品安全的重视以及中国经济与国际市场的逐渐接轨,我国现行的沙门氏菌限量标准已不能满足保护消费者健康的需求,且不利于增强我国食品的进...由于沙门氏菌引起的食物中毒不仅危害人们的身体健康,而且还会造成严重的经济损失。随着人们对食品安全的重视以及中国经济与国际市场的逐渐接轨,我国现行的沙门氏菌限量标准已不能满足保护消费者健康的需求,且不利于增强我国食品的进出口贸易竞争力。本文总结了中国和国际食品微生物标准委员会(International Commission of Microbiological Specializations on Food,ICMSF)、国际食品法典委员会(Codex Alimentarius Commission,CAC)、欧盟(European Union,EU)、美国、日本、加拿大以及澳大利亚的食品卫生标准中沙门氏菌限量标准的异同,以期为我国制定与国际接轨的沙门氏菌限量标准提供借鉴。展开更多
目的：由于中国药典中规定的沙门菌检查采用微生物培养法,其操作繁琐、培养周期长,本研究拟建立一种快速定性检测沙门菌的方法以替代药典中繁琐耗时的微生物培养法。方法：取10 m L含动物类药材的口服制剂,分别加入0.096~96 cfu的沙门菌...目的：由于中国药典中规定的沙门菌检查采用微生物培养法,其操作繁琐、培养周期长,本研究拟建立一种快速定性检测沙门菌的方法以替代药典中繁琐耗时的微生物培养法。方法：取10 m L含动物类药材的口服制剂,分别加入0.096~96 cfu的沙门菌,同时以大肠埃希菌作为干扰对照菌,设置沙门菌污染组、大肠埃希菌污染组、沙门菌及大肠埃希菌混合污染组及阴性对照组共4个实验组,采用多重聚合酶链扩增技术（PCR）对供试品溶液进行扩增检测,分别考察该方法对沙门菌检出的专属性、准确性、灵敏度以及适用性。结果：所建立的方法检验周期短,仅需30小时;专属性好,能准确区分沙门菌与干扰对照菌;结果准确,检测结果与药典方法检验结果一致;灵敏度高,最低检测限为1 cfu。结论：本方法便捷高效、结果准确,可为药品检验中的沙门菌检查提供一种新手段。展开更多
文摘Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance （SPR） biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically ＂taste＂ the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif- ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con- centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 x 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.
文摘由于沙门氏菌引起的食物中毒不仅危害人们的身体健康,而且还会造成严重的经济损失。随着人们对食品安全的重视以及中国经济与国际市场的逐渐接轨,我国现行的沙门氏菌限量标准已不能满足保护消费者健康的需求,且不利于增强我国食品的进出口贸易竞争力。本文总结了中国和国际食品微生物标准委员会(International Commission of Microbiological Specializations on Food,ICMSF)、国际食品法典委员会(Codex Alimentarius Commission,CAC)、欧盟(European Union,EU)、美国、日本、加拿大以及澳大利亚的食品卫生标准中沙门氏菌限量标准的异同,以期为我国制定与国际接轨的沙门氏菌限量标准提供借鉴。
文摘目的：由于中国药典中规定的沙门菌检查采用微生物培养法,其操作繁琐、培养周期长,本研究拟建立一种快速定性检测沙门菌的方法以替代药典中繁琐耗时的微生物培养法。方法：取10 m L含动物类药材的口服制剂,分别加入0.096~96 cfu的沙门菌,同时以大肠埃希菌作为干扰对照菌,设置沙门菌污染组、大肠埃希菌污染组、沙门菌及大肠埃希菌混合污染组及阴性对照组共4个实验组,采用多重聚合酶链扩增技术（PCR）对供试品溶液进行扩增检测,分别考察该方法对沙门菌检出的专属性、准确性、灵敏度以及适用性。结果：所建立的方法检验周期短,仅需30小时;专属性好,能准确区分沙门菌与干扰对照菌;结果准确,检测结果与药典方法检验结果一致;灵敏度高,最低检测限为1 cfu。结论：本方法便捷高效、结果准确,可为药品检验中的沙门菌检查提供一种新手段。
