Multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

基于改进YOLOv3-SPP算法的道路车辆检测

针对在城市道路场景下视觉检测车辆时,车辆密集和远处车辆呈现小尺度,导致出现检测精度低或者漏检的问题,提出了一种基于改进的YOLOv3-SPP算法,对激活函数进行优化,以DIOU-NMS Loss作为边界框损失函数,增强网络的表达能力。为提高所提算法对小目标和遮挡目标的特征提取能力,引入空洞卷积模块,增大目标的感受野。实验结果表明,所提算法在检测车辆目标时m AP提高了1.79%,也有效减少了在检测紧密车辆目标时出现的漏检现象。

FOSB、SPP1基因表达对经肝动脉介入化疗栓塞术治疗的中晚期肝癌生存期预测价值

目的探讨骨肉瘤原癌基凶同源物B(FOSB)、分泌性磷蛋白1(SPP 1)基因表达对经肝动脉介入化疗栓塞术治疗的中晚期肝癌患者术后生存期的预测价值。方法回顾性分析2016年1月—2017年10月在西安医学院第一附属医院进行诊治的中晚期肝癌患者115例作为研究对象,根据FOSB、SPP 1基因表达水平分为FOSB高表达组(n=52)、FOSB低表达组(n=63)及SPP 1高表达组(n=89)、SPP 1低表达组(n=26)。同时选取115例健康体检者为健康对照组。分析FOSB、SPP 1表达与中晚期肝癌患者临床病理特征的关系。对纳入研究的患者进行为期60个月的随访,Logistics风险回归模型分析影响患者生存期的危险因素。Kaplan-Meier生存曲线分析FOSB、SPP 1表达水平与患者生存预后的关系。结果肝癌患者外周血单个核细胞中FOSB mRNA表达水平明显低于健康对照组,SPP 1 mRNA表达水平明显高于健康对照组,差异具有统计学意义(P<0.05)。FOSB、SPP 1表达在中晚期肝癌患者肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期方面比较差异具有统计学意义(P<0.05)。单因素分析结果显示,存活组和死亡组在肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期、FOSB、SPP 1表达方面比较,差异均具有统计学意义(P<0.05)。肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期、FOSB低表达、SPP 1高表达均为中晚期肝癌患者生存期的独立危险因素(P<0.05)。随访时间60个月,患者生存率为17.39%(20/115),FOSB高表达组中位生存时间为39.7个月,明显高于FOSB低表达组的19.3个月(P<0.05);SPP 1低表达组中位生存时间为40个月,明显高于SPP 1高表达组的18个月(P<0.05)。结论FOSB在中晚期肝癌患者中表达明显下调,SPP 1表达上调,其对预测中晚期肝癌患者肝动脉介入化疗栓塞术治疗生存期具有一定的价值。FOSB、SPP 1有望成为评估介入术治疗患者预后的潜在指标,可协同肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期等临床指标预测或评估肝癌患者术后生存情况。

Effects of Sodium Lactate on the Survival of <i>Listeria Monocytogenes</i>, <i>Escherichia coli</i>O157:H7, and <i>Salmonella</i>spp. in Cooked Ham at Refrigerated and Abuse Temperatures

The objective of this study was to determine the effect of sodium lactate on the survival of Listeria monocytogenes, Escherichia coli O157: H7, and Salmonella spp. in cooked ham during storage at refrigerated and abuse temperatures. Cooked ham was added with 0% - 3% lactate, inoculated with a multiple-strain mixture of L. monocytogenes, E. coli O157: H7, or Salmonella spp. and stored at 4oC - 15oC for up to 35 day. The growth of the three pathogens was inhibited in ham containing 3% lactate, and no growth of E. coli O157: H7 and Salmonella spp. occurred at the lowest storage tem- peratures of 6 and 8oC, respectively. In ham containing no lactate, the average growth rates were 0.256 - 0.380 log CFU/day for L. monocytogenes at 4oC - 8oC, 0.242 - 0.315 log CFU/day for E. coli O157: H7 at 8oC - 15oC, and 0.249 - 0.328 log CFU/day for Salmonella spp. at 10oC - 15oC. The addition of 1% or 2% lactate significantly (P < 0.05) reduced the growth rates of the three pathogens, and the effect was more profound at lower temperatures. Salmonella spp. were more sensitive to the effect of lactate than L. monocytogenes and E. coli O157: H7. Polynomial models were developed to describe the growth rates of the three pathogens as affected by the lactate concentration and storage tem- perature. Results from this study demonstrate the effect of lactate on the growth of L. monocytogenes, E. coli O157: H7, and Salmonella spp. in cooked ham and indicate the effective lactate concentrations and storage temperatures that can be used to enhance the microbiological safety of ready-to-eat ham products.

Prevalence and antimicrobial resistance of Salmonella spp.in raw retail frozen imported freshwater fish to Eastern Province of Saudi Arabia

Objective:To determine the proportion of imported frozen fish contaminated with Salmonella among retail food stores and supermarkets in the Eastern Province of Saudi Arabia.Methods:A total of 223 frozen freshwater fish purchased from different supermarkets and grocery stores were analyzed for the presence of foodborne pathogen Salmonella.The isolation of Salmonella was determined and confirmed by using the methods of US Food and Drug Administration's Bacteriological Analytical Manual.CHROMagar Salmonella plus,biochemical tests and API 20E strips.Antimicrobial susceptibilities of Salmonella isolates were determined by the disk diffusion method on Muller-Hinton agar,as described by Kirby-Bauer.in accordance with the guidelines of the Clinical and Laboratory Standards Institute.Results:Out of the total 223 fish samples(20 of catfish,18 of carfu,20 of mirgal,25 of milkfish,35 of mackerel,75 of tilapia,and 30 of rohu),89(39.9%)were tested positive for Salmonella.The prevalence of positive samples were reported for the freshwater fish of pangas(60.0%,n=12),carfu(27.7%,n=5),mirgal(35.0%,n=7),milkfish(52.0%,n=13),mackerel(31.4%,n=11),tilapia imported from Thailand(64.0%,n=16),tilapia imported from India(28.0%,n=14),rohu imported from Thailand(26.6%,n=4)and rohu imported from Myanmar(46.6%,n=7).A total of 140 isolates of Salmonella spp.were yielded from at least seven different types of frozen freshwater fish imported from 5 different countries and were tested for their susceptibility to 16 selected antimicrobial agents.The highest antibiotic resistance was observed to tetracycline(90.71%)followed by ampicillin(70%)and amoxicillin-clavulanic acid(45%).Conclusions:The obtained results of this study shows that these raw retail imported frozen freshwater fish are contaminated with potentially pathogenic Salmonella spp.And the study recommend and suggest that there is a need for adequate consumer measures.

Bovine salmonellosis in Northeast of Iran:Frequency,genetic fingerprinting and antimicrobial resistance patterns of Salmonella spp.

Objective:To evaluate serovar and antimicrobial resistance patterns of Salmonella spp isolated from healthy,diseased and necropsied cows and calves in this observational study.Methods:Nineteen isolates recovered from feces and tissues of salmonellosis-affected animals of two commercial farms in north-east of Iran.In second part of the study,the two farms were sampled 4 times with an interval of 2 month.The samples included calves'feces,adult cows'feces,feeds,water,milk filters,and milk fed to calves.Five Salmonella were isolated from 332fecal samples collected from calves and peri-parturient cows.No Salmonella was recovered from water,feed,milk filers and milk fed to calves.Results:Salmonella Typhimurium was the most frequently isolate among all sero-groups.S.Dublin was only accounted for 8%(two out of 24)of isolates.Isolated Salmonella strains were used for the ERIC PCR DNA fingerprinting assay.Our results grouped Salmonella isolates into 3 clusters,suggesting that specific genotypes were responsible for each sero-group of Salmonella.The results also revealed diversity among Salmonella isolates in cluster III(sero-group B).Eighteen out of 19 Salmonella spp.were resistant to oxytetracycline.Five isolates out of 19 showed more than one drug resistance.Multi-drug resistance was seen only among Salmonella Typhimurium isolates.Enrofloxacin was the most susceptible antibiotic against all isolates in this study.Conclusion:The emergence of multiple antibiotic-resistant strains of Salmonella Typhimurium should be of great concern to the public.No correlation between ERIC fingerprinting and resistance patterns of Salmonella isolates was found,which indicates resistance to antimicrobial agents was not related to specific genetic background.

Food Safety and Antimicrobial Resistance an Approach to the Genus <i>Salmonella</i>spp.

Foodborne Illnesses (FI) are considered an important problem in public health for their high levels of morbidity and, in some cases, of mortality in Mexico and around the world. Different agents that cause Foodborne Illnesses, in which biological agents such as the genus Salmonella spp., are included, have been often associated with outbreaks. A continued effort has been observed in the food industry, in collaboration with sanitary authorities on a global scale, through the creation and continued improvement of different procedures to prevent the contamination by Salmonella, in which the elaboration of laboratory methodologies for the detection and isolation of this pathogen in foods and, in such manner, prevents the outbreak of illnesses. However, along with the aforementioned, it has been reported that a few years ago, the appearance of an ever-increasing number of strains of Salmonella spp., in foods with multi-drug resistance to antibiotics which are used in the treatment of its illness, results in a major emphasis on the health issue related to Foodborne Illnesses and, in particular, to those generated by factors of medical dependence such as the increase in recuperation time, costs, and reduction in the number of alternative pharmaceutical treatments. This work presents a general overview of FI, in which illnesses generated by bacteria of the genus Salmonella spp., their detection in a microbiological laboratory, as well as the phenomenon of resistance to antibiotics by these bacteria, and the current and difficult issue reported through different investigations all around the world, of the rise in this phenomenon and its importance in public health, are discussed.

Modeling the Surface Cross-Contamination of <i>Salmonella</i>spp. on Ready-to-Eat Meat via Slicing Operation

Salmonella spp. surface transfer patterns/models between ready-to-eat (RTE) deli meat and a typical meat slicer are presented. A five-strain cocktail of Salmonella spp. was inoculated directly onto the rim of a round slicer blade at an initial level of ca. 4, 5, 6, 7 or 8 log CFU/blade (ca. 3, 4, 5, 6 or 7 log CFU/cm2 of the blade edge area). Thereafter, a RTE deli meat (ham) was sliced to a thickness of 1.5 - 2.0 mm. In another cross-contamination scenario, a clean blade was initially used to slice ham, which was pre-surface-inoculated with the Salmonella cocktail (ca. 2, 3, 4, 5, 6 or 7 log CFU/cm2 with a total area of 100 cm2), followed by slicing of an un-inoculated ham. The log CFU of Salmonella per ham slice was determined and empirical models were developed. The models, follow the decreasing Power law, predict the surface cross-contamination of Salmonella spp. (at any initial level) for sliced deli meat (ham) and will provide a useful tool in developing RTE meat risk assessments. Surface transfer patterns of three foodborne pathogens, i.e. Listeria monocytogenes, E. coli O157:H7 and Salmonella predicted by models are also presented.

Presence of Multidrug-Resistant <i>E. coli</i>, <i>Enterococcus</i>spp. and <i>Salmonella</i>spp. in Lakes and Fountains of Porto, Portugal

Urban lakes and fountains provide recreational activities that could facilitate the contact between humans, animals and biological agents. The objective of this work was to assess the water quality and safety of 17 lakes and 13 fountains in the city of Porto (Portugal), by detecting the presence of Escherichia coli, enterococci and Salmonella spp., and analyzing their antimicrobial resistance. (For more information,please refer to the pdf.)

Simultaneous Detection of <i>Salmonella</i>spp., <i>Salmonella</i>Enteritidis and <i>Salmonella</i>Typhimurium in Raw Salad Vegetables and Vegetarian Burger Patties

The health risks posed by Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium through the consumption of raw vegetables and vegetarian burger patties necessitates the needs for the optimization of analytical approach for their detection and enumeration in the raw vegetables, which served as potential vehicles for transmission of these pathogenic microorganisms. We sought to establish a rapid, economic and sensitive method to detect and determine the load of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium using the most probable numbers (MPN) in combination with the multiplex polymerase chain reaction (MPCR). From the naturally contaminated one hundred and seventy five samples tested (n = 175), the overall prevalence of Salmonella spp. was 28%, Salmonella Enteritidis was 20% and Salmonella Typhimurium was 14.3%, respectively. The MPN-MPCR is a quantitative method to determine the density of cell concentration of Salmonella in all the samples (Salmonella spp. ranged from <3 to 53 MPN/g;S. Enteritidis ranged from <3 to 24 MPN/g;and S. Typhimurium ranged from <3 to 15 MPN/g). The combination of the MPN-MPCR is an efficient, simple, fast analytical method for the detection and enumeration of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in vegetables and the vegetarian burger patties since it can significantly reduce time and labour with analysis completed within 2 days, as opposed to the traditional confirmation method that can take up to 5 days for unequivocal identification of species.

Evaluation of the Microbiological Quality of Poultry Imported into Togo and the Antibiotic Resistance of Salmonella spp. Isolated

In Togo, despite the government’s efforts, food requirements in terms of animal proteins are not covered by national production and are subject to huge imports of meat products. However, the hygienic quality of these imports is not guaranteed for the consumer. The aim of this study was to estimate the frequency of unhygienically unsatisfactory imported poultry and to determine the antibiotic resistance profile of Salmonella spp. strains. A total of 285 samples of imported poultry, including 55 chicken thighs, 10 chicken backbones, 25 chicken wings, 5 whole chickens, 30 sausages, 35 chicken forequarters, 95 chicken drumsticks and 30 guinea fowl wings, were analyzed using standard AFNOR routine methods. The following germs were tested: Total Aerobic Mesophilic Flora (TAMF), Anaerobic-Sulfite-Reducing (ASR), Escherichia coli, Staphylococcus aureus and Salmonella spp. Antibiotic susceptibility testing was carried out on Salmonella spp. strains isolated using the agar disk diffusion method (CA-SFM). Results showed 100% compliance for TAMF, coagulase-positive Staphylococci and Escherichia coli. On the other hand, 3.84% and 2.46% non-compliance were recorded for ASR and Salmonella respectively. Non-compliance with hygiene rules is generally thought to be the cause of meat contamination. Seven 7 strains of Salmonella were isolated, 5 of which were of the OMA serogroup, and the other two of the OMB and HMB groups. Antibiotic susceptibility tests revealed resistance to certain beta-lactam antibiotics and quinolones, in particular: cefalexin (28.57%), cefoxitin (14.28%), cefuroxime (28.57%), ceftazidime (28.57%), ceftriaxone (28.57%) and nalidixic acid (28.57%). This result may be explained by the uncontrolled use of B-lactam and quinolone antibiotics in poultry farming. As Salmonella spp. is a pathogenic enteric bacterium that causes food-borne illness in humans, resistance to third-generation cephalosporins remains a major public health problem.

Monitoring of Escherichia coli, Salmonella spp. and Staphylococci in Poultry Meat-Based Fast Food in Saudi Arabia

An investigation was made to survey the possible presence of Staphylococcus aureus, Escherichia coli and Salmonella spp. from fast-food shops in Al-Ahsa Province, Kingdom of Saudi Arabia (KSA), as potential reservoir of human infection and antimicrobial resistance. A total of 100 samples of shawarma poultry meat were collected from different localities of the province. Conventional, commercial VITEK 2 and molecular techniques were used for isolates’ identification and antibiogram detection. Staph aureus was isolated at a rate of 14% and CNS as Staph. sciuri and Staph. xylosus at 2%. E. coli was identified at a rate of 12% and antibiogram analysis showed 41.67% of isolates to be extended-spectrum β-lactamases (ESBL) with evidence of multi-drug resistance (MDR). Molecular analysis of E. coli revealed presence of sero-groups O1 and O2, entero-toxigenic (ETEC), shiga-toxigenic, ST540 and the prototypical ETEC strain H10407 which are potential public health hazard. Salmonella enterica serovar Enteritidis showed 19% prevalence while S. Typhimurium with 8% prevalence. Anti-microbial sensitivity of 15 strains of S. Enteritidis and 5 strains of S. Typhimurium showed multi-drug resistance (MDR).

Potential Risk of Transmission of Escherichia coli and Salmonella spp. Infections by the Musca domestica Fly and the Periplaneta americana Cockroach in the City of Cotonou (South Benin)

Background: The occurrence of bacterial infections sometimes involves synanthropic flies and cockroaches, as mechanical vectors of pathogenic microorganisms. The aim of this study was to identify the different species of flies and cockroaches that cohabit with humans in the city of Cotonou and determine the contribution of Musca domestica and Periplaneta americana in the transmission of bacteria responsible for infections in humans. Methods: Capture sessions during the day for flies and at night for cockroaches were carried out in 4 arrondissements of the city of Cotonou. The insects collected were transported to the Center of Research Entomological of Cotonou for identification, then sent to the Section Hygiene of Water and Food for microbiological analysis, enumeration and testing for human pathogenic bacteria. Results: A total of 351 flies and 26 cockroaches were collected at the sites, including two synanthropic species belonging to two families each. Musca domestica and Periplaneta americana were the most abundant species found after identification in the city of Cotonou. Thermo-tolerant coliforms and Escherichia coli were found on all specimens at varying concentrations. Salmonella spp. bacteria were identified in the fly population from the Dantokpa dump. Conclusion: Flies and cockroaches can transmit microorganisms to humans. The presence of Salmonella spp. among the specimens revealed that infections frequently caused by contaminated food or water are also transmitted to humans by flies. The presence of these germs on flies and cockroaches represents a potential risk of mechanical transmission to humans. It is, therefore, essential to continue investigations in order to assess the species of bacteria propagated, improve control strategies against these troublesome insects and adopt better hygiene conditions for better living.

Combination of a <i>Lactobacillus</i>-Based Probiotic and Organic Acids Decrease Egg to Chick Weight Loss and Reduce <i>Salmonella</i>spp. Counts in the Litter of Commercial Broiler Breeders

The effects of supplementing a blend of organic acids (OA) and a lactic acid bacteria (LAB) based-probiotic on egg to chick weight loss (%) and Salmonella spp. recovery counts in the litter of commercial broiler breeders were examined in three independent trials during the years 2013, 2014 and 2015. In each trial, ten thousand birds were divided into two groups of 5000 birds (4500 females and 500 males in each group): Control non-treated group, receiving regular water or treated group, receiving OA and probiotic in the drinking water from 25 to 35 weeks of age. During the ten weeks of evaluation, one thousand fertile eggs or hatched chickens in each trial respectively, were evaluated to obtained, hatching set weight, transferred egg weight, and hatching chick weight, to estimate the difference between egg to chick weight loss (%). Besides, in trial 2 (2014) and trial 3 (2015), litter samples were collected to evaluate Salmonella spp. counts. The supplementation of OA and probiotic during ten consecutive weeks significantly decreased the egg to chick weight loss (%) when compared with the control non-treated groups in all three trials evaluated. Interestingly, at the end of the trials 2 and 3, no counts of Salmonella spp. in the litter were detected. In contrast, control-non treated groups resulted in 4.30 and 4.24 Log10 of Salmonella spp. in trials two and three respectively. The results of the present study suggest that supplementation of OA and a LAB-probiotic for ten consecutive weeks decrease the egg to chick weight loss (%) and reduce Salmonella spp. counts in the litter of commercial broiler breeders. Higher initial body weight in broiler chickens and reduction of Salmonella spp., clearly justify the use of the combination of these products, as alternatives to antibiotics.

Prevalence and Antibiotic Resistance of <i>Salmonella</i>spp. in Turkey

The current study was conducted to investigate the prevalence of Salmonella spp. in turkey and to determine the antimicrobial resistance pattern of the isolated Salmonellae. Two hundred and fifty turkeys were randomly selected for cloacal soab samples preparation, and the samples were investigated for Salmonella isolation. Identification of the isolated Salmonella was performed using standard bacteriological and biochemical procedures. The prevalence of Salmonella in turkey was about 14.8%. Disc diffusion tests on Muller-Hinton agar were used to determine the sensitivity to antibacterial agents. Ten antibiotics were studied: lincospectin, colistin, cephalexin, ciprofloxacin, chloramphenicol, gentamycin, furazolidone, streptomycin, co-trimoxazole (trimethoprim-sulfamethoxazole) and tetracycline. The highest resistant was observed against cephalexin (89.2%), tetracycline (86.5%), colistin (83.8%), and furazolidone (73%). The Highest sensitivity was found to gentamycin (86.5%), ciprofloxacin (83.8%), chloramphenicol (51.4%) and streptomycin (40.6%). The results showed high prevalence of Salmonella spp. in turkey and high levels of antimicrobial resistance pattern of the isolated Salmonellae were observed.

Different Species of Salmonella spp and Shigella spp and Their Risk of Infection in N’Djamena Chad

Introduction: Salmonella and Shigella are gram-negative bacilli that are resistant to most antibiotics and play an important role in an etiology of diarrhea disease. The aim of the study was to determine the prevalence of Salmonella spp and Shigella spp isolated from stool and blood samples in the city of N’Djamena. Materials and Method: This was a prospective study conducted in the four district hospitals of N’Djamena from 14 July 2022 to 31 December 2022. A questionnaire form was drawn up to collect the information sent to the study patients. The samples were analyzed at the CHU de la Mère et de l’Enfant, Labo-Redes laboratory according to their protocols and the standard of the antibiogram committee of the French microbiology society. Results: Of the 803 biological samples analyzed, 39 were positive for Salmonella spp and Shigella spp, including 15 for Salmonella and 24 for Shigella, giving an overall prevalence rate of 4.85%. Borehole water, uncooked food and lack of access to a latrine constitute a risk of being infected by Salmonella spp and Shigella spp species. Of the 8 antibiotics tested, Salmonella spp and Shigella spp strains showed good sensitivity to nalidixic acid (100% for Salmonella and 90 for Shigella) and to ciprofloxacin (90.9% for Salmonella and 75% for Shigella). Resistance to ampicillin was found in 81.81% of Salmonella species and 78.57% of Shigella species, as was resistance to chloramphenicol (81.81% of Salmonella species and 67.85% of Shigella species). Similarly, cleanliness of the service and equipment is an essential factor in preventing Salmonella and Shigella infections.

SPP1和PD-L1在子宫内膜癌组织中的表达及其预后价值

目的:分析分泌型磷蛋白1(secreted phosphoprotein 1,SPP1)和程序性死亡蛋白配体1(programmed cell death-ligand 1,PD-L1)在子宫内膜癌(endometrial carcinoma,EC)组织中的表达水平及与预后的关系。方法:通过免疫组织化学方法分析SPP1和PD-L1在84例EC组织和61例正常子宫内膜组织中的表达情况,分析SPP1和PD-L1表达与临床病理特征及预后的关系,利用UALCAN分析工具(https://ualcan.path.uab.edu/)分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中EC组织SPP1和PD-L1 mRNA的表达及与预后的关系。结果:EC组织SPP1阳性表达率高于正常子宫内膜组织(71.43%vs 36.07%,P=0.001),PD-L1阳性表达率低于正常子宫内膜组织(25.00%vs 42.62%,P=0.025);EC组织中,SPP1阳性表达率与组织学分级相关(P<0.05),PD-L1阳性表达率与淋巴结转移相关(P<0.05),SPP1与PD-L1表达呈正相关(列联系数r=0.237,P=0.026);EC组织中,不同SPP1和PD-L1表达患者总生存期无差异(均P>0.05)。TCGA数据库中生物信息学分析显示EC中SPP1 mRNA表达水平显著高于正常子宫内膜组织(P<0.01),PD-L1 mRNA表达水平与正常子宫内膜组织比较,差异无统计学意义(P=0.057);EC组织SPP1 mRNA和PD-L1 mRNA表达水平呈正相关(r=0.350,P<0.05);不同SPP1和PD-L1表达水平患者总生存期无差异(均P>0.05)。结论:SPP1在EC组织中的表达与PD-L1呈正相关,可能共同参与EC免疫微环境调控,SPP1和PD-L1均不是EC独立预后因子。

Prevalence and Predictors of Salmonella spp. in Retail Meat Shops in Kathmandu

A cross-sectional study was conducted from November 2008 to May 2009 to estimate the prevalence of Salmonella in retail meat shops in Kathmandu. The methods followed were ISO 18593：2004 for swab sample collection, ISO 6579：2002 for Salmonella isolation and manufacturer＇s instructions （SIFIN, Germany） for serotype identification. A questionnaire was used to collect information on some of the risk factors of shops likely to be associated with Salmonella identification. A total of 492 environmental swab samples （164 chopping board samples, 164 knife samples and 164 table samples） from 82 retail meat shops were analyzed. The prevalence of Salmonella positive shops was 40.2% （95% CI： 29-51）. The isolation rates of Salmonella from chopping boards （36.0%）, knives （32.9%） and tables （25.0%） were not significantly different （P 〉 0.05）. Retail meat shops were 1.9 times more likely to yield Salmonella in the evening （38.2%） as compared to the morning （24.4%） （P = 0.001）. S. Typhimurium （54.5%） was the most common serotype found in retail meat shops followed by S. Enteritidis （16.9%）, S. Haifa （13.6%）, S. Virchow （10.4%）, S. Agona （3.9%） and S. enterica （0.6%）. Among the risk factors examined, ＂hygiene status of shop＂, ＂type of shops＂, ＂number of person handling meats＂, ＂number of knives used＂, ＂number of kinds of meat sold＂ and ＂number of kinds of meat sold using different numbers of knives＂ were individually significantly （P 〈 0.05） associated with Salmonella contamination in the retail meat shops. After univariate analysis of these risk factors, a final logistic regression model with Salmonella yes or no category of shops as outcome variable identified four significant predictors. Odds ratios, indicating the likelihood increase of a shop to achieve Salmonella positivity status were 10.17 for multiple persons rather than a single person involved, 7.66 for open rather than closed shops, 9.44 for use of several knives rather than one knife and 5.18 for single kind of meat using several knives. The results of this investigation revealed that retail meat shops to a noticeable extent are Salmonella contaminated, with a considerable degree of cross-contamination between meats and personnel and equipment used during a day in processing of meats.

Antimicrobial Susceptibilities of Salmonella spp. Isolated from Chicken Meat Samples

The aim of this study was to determine presence of Salmonella spp. in chicken meat samples collected from Ankara, Turkey and determine the susceptibility of the Salmonella isolates to some antimicrobial agents. For this purpose, 127 chicken samples were collected from local markets. Investigation of Salmonella was done according to horizontal method, the guidelines of the method recommended by International Standards Organization （ISO）. Antimicrobial susceptibilities of Salmonella spp. was performed with microdilution method according to the guidelines of CLSI M100-SI8. Ampicillin, gentamicin sulphate, ofloxacin, levofloxacin, ciprofloxacin, enrofloxacin, tetracycline, ceftriaxon, amoxicillin/clavulanic acid and trimethoprim/sulfamethoxazole were used in the study. Salmonella spp. was isolated from 5 （3.94%） of the 127 chicken meat samples. Although our findings are not within Turkish Food Codex （TFC） values, lower number of samples that involve Salmonella spp. indicates an improvement in the hygienic conditions in Turkey. Among 5 isolates, one isolate was sensitive to all antimicrobial agents tested. 2 isolates exhibited multidrug resistance. Successfully, all the isolates were sensitive to quinolones as a good result in spite of the reported reduced susceptibility from different regions of the world. However, this study should be improved with more chicken samples and Salmonella spp. isolate numbers to support these results.

Genetic Diversity of Jute Mallow (Corchorus spp.) Accessions Based on ISSR Markers

Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.