A global quality control method based on high performance liquid chromatography(HPLC)coupled with diode array detection(DAD),single quadrupole mass spectrometry(MS)and time-of-flight mass spectrometry(TOFMS)was develo...A global quality control method based on high performance liquid chromatography(HPLC)coupled with diode array detection(DAD),single quadrupole mass spectrometry(MS)and time-of-flight mass spectrometry(TOFMS)was developed for simultaneous determination of seven major components(mangiferin,neomangiferin,timosaponin E1,timosaponin E,timosaponin BⅡ,timosaponin BⅢ,and timosaponin AⅢ)and identification of most components in extracts of Rhizoma Anemarrhenae(RA).HPLC analysis was performed on an Agilent SB-C18 column(4.6 mm×150 mm,5 μm)by gradient elution using acetonitrile and water-acetic acid(100∶0.05,v/v)as the mobile phase.Seven major components in RA were successfully separated.This quantitative method was fully validated in respect of the following performance criteria:linearity,precision,repeatability,stability,accuracy,limits of detection(LOD)and quantification(LOQ).A formula database of known compounds in RA was established,against which,most of the reported components in this herbal extract were identified effectively based on the extract masses acquired by TOFMS.This qualitative and quantitative method was successfully used to analyze the components in 10 batches of RA samples collected from different regions in China.This global quality control method,which consisted of HPLC-DAD-MS assay of seven major components and unambiguous identification of nineteen components,is suitable for routine quantification and comprehensive quality control of RA.展开更多
Objective:To investigate the effect of Rhizoma Anemarrhenae extract on glucose and lipid metabolism in diabetic mice,and provide basis for follow-up research.Methods:The method of streptozotocin combined with high-fat...Objective:To investigate the effect of Rhizoma Anemarrhenae extract on glucose and lipid metabolism in diabetic mice,and provide basis for follow-up research.Methods:The method of streptozotocin combined with high-fat diet was used to replicate the diabetes model in this study.After continuous intragastric administration for 6 weeks,the effects of Rhizoma Anemarrhenae extract on body weight,blood sugar,blood lipids,serum insulin and the organ index in diabetic mice were detected.Results:The fasting blood glucose and blood lipids(serum total cholesterol,triglyceride,low density lipoprotein cholesterol levels)of the Rhizoma Anemarrhenae group of mice were significantly decreased,which was statistically significant compared with the model group.The area under the curve of the Rhizoma Anemarrhenae group is significantly smaller than that of the model group,and the result is statistically significant.Rhizoma Anemarrhenae can increase serum insulin levels in diabetic mice,which is statistically significant compared with the model group.Compared with the model group,the liver,spleen,kidney and pancreas indexes of the Rhizoma Anemarrhenae group are significantly reduced.Conclusion:The extract of Rhizoma Anemarrhenae can significantly reduce blood glucose concentration,lower blood lipid levels,promote serum insulin secretion,and protect liver,kidney,spleen,pancreas and other tissues and organs.展开更多
目的采用化学计量学结合灰色关联度—逼近理想解排序(technique for order preference by similarity to an ideal solution,TOPSIS)法模型建立综合评价知母药材质量的方法。方法建立高效液相色谱法(high performance liquid chromatogr...目的采用化学计量学结合灰色关联度—逼近理想解排序(technique for order preference by similarity to an ideal solution,TOPSIS)法模型建立综合评价知母药材质量的方法。方法建立高效液相色谱法(high performance liquid chromatography,HPLC)黄酮特征图谱及水分、总黄酮、总皂苷含量测定方法,采用聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法—判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)对不同批次知母样品进行化学计量学分析,利用熵权法结合灰色关联度—TOPSIS构建知母药材质量评价模型。结果建立的知母黄酮特征图谱、含量测定方法稳定、可靠;HCA、PCA和OPLS-DA可将10批知母样品分为2类;灰色关联度—TOPSIS模型综合得分显示,S10号知母样品质量较好,评分靠前。结论所建立的特征图谱结合化学计量学与灰色关联度—TOPSIS分析方法,能客观评价不同批次知母药材质量,为中药质量综合评价与控制的方法学建立提供参考。展开更多
基金supported by the National Science and Technology Supporting Program(No.2006BAI08B03-07)the National Natural Science Foundation of China(No.30873196)
文摘A global quality control method based on high performance liquid chromatography(HPLC)coupled with diode array detection(DAD),single quadrupole mass spectrometry(MS)and time-of-flight mass spectrometry(TOFMS)was developed for simultaneous determination of seven major components(mangiferin,neomangiferin,timosaponin E1,timosaponin E,timosaponin BⅡ,timosaponin BⅢ,and timosaponin AⅢ)and identification of most components in extracts of Rhizoma Anemarrhenae(RA).HPLC analysis was performed on an Agilent SB-C18 column(4.6 mm×150 mm,5 μm)by gradient elution using acetonitrile and water-acetic acid(100∶0.05,v/v)as the mobile phase.Seven major components in RA were successfully separated.This quantitative method was fully validated in respect of the following performance criteria:linearity,precision,repeatability,stability,accuracy,limits of detection(LOD)and quantification(LOQ).A formula database of known compounds in RA was established,against which,most of the reported components in this herbal extract were identified effectively based on the extract masses acquired by TOFMS.This qualitative and quantitative method was successfully used to analyze the components in 10 batches of RA samples collected from different regions in China.This global quality control method,which consisted of HPLC-DAD-MS assay of seven major components and unambiguous identification of nineteen components,is suitable for routine quantification and comprehensive quality control of RA.
基金This study was supported by the Key Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine in Gansu Province(ZDSYS-KJ-2018-007)the 2020 Gansu Provincial Higher Schools Industry Support and Guidance Project(2020C-9)Gansu University of Chinese Medicine Introduced Talent Research Startup Fund Project(2016YJRC-03,2016YJRC-06).
文摘Objective:To investigate the effect of Rhizoma Anemarrhenae extract on glucose and lipid metabolism in diabetic mice,and provide basis for follow-up research.Methods:The method of streptozotocin combined with high-fat diet was used to replicate the diabetes model in this study.After continuous intragastric administration for 6 weeks,the effects of Rhizoma Anemarrhenae extract on body weight,blood sugar,blood lipids,serum insulin and the organ index in diabetic mice were detected.Results:The fasting blood glucose and blood lipids(serum total cholesterol,triglyceride,low density lipoprotein cholesterol levels)of the Rhizoma Anemarrhenae group of mice were significantly decreased,which was statistically significant compared with the model group.The area under the curve of the Rhizoma Anemarrhenae group is significantly smaller than that of the model group,and the result is statistically significant.Rhizoma Anemarrhenae can increase serum insulin levels in diabetic mice,which is statistically significant compared with the model group.Compared with the model group,the liver,spleen,kidney and pancreas indexes of the Rhizoma Anemarrhenae group are significantly reduced.Conclusion:The extract of Rhizoma Anemarrhenae can significantly reduce blood glucose concentration,lower blood lipid levels,promote serum insulin secretion,and protect liver,kidney,spleen,pancreas and other tissues and organs.
文摘目的采用化学计量学结合灰色关联度—逼近理想解排序(technique for order preference by similarity to an ideal solution,TOPSIS)法模型建立综合评价知母药材质量的方法。方法建立高效液相色谱法(high performance liquid chromatography,HPLC)黄酮特征图谱及水分、总黄酮、总皂苷含量测定方法,采用聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和正交偏最小二乘法—判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)对不同批次知母样品进行化学计量学分析,利用熵权法结合灰色关联度—TOPSIS构建知母药材质量评价模型。结果建立的知母黄酮特征图谱、含量测定方法稳定、可靠;HCA、PCA和OPLS-DA可将10批知母样品分为2类;灰色关联度—TOPSIS模型综合得分显示,S10号知母样品质量较好,评分靠前。结论所建立的特征图谱结合化学计量学与灰色关联度—TOPSIS分析方法,能客观评价不同批次知母药材质量,为中药质量综合评价与控制的方法学建立提供参考。
