To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,...To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.展开更多
Objective:To explore the molecular biological mechanism of the"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer based on network pharmacology.Methods:Searching the TCMSP...Objective:To explore the molecular biological mechanism of the"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer based on network pharmacology.Methods:Searching the TCMSP database and previous literatures to screen the active compounds which resist lung cancer activity in salvia chinensis and radix ranunculi ternati.The candidate compounds were unified in the DrugBank to find the corresponding drug targets which were corrected to the standard gene names by the UniProt database.The Swiss Target Prediction platform was used to predict other targets.Searching GeneCards,OMIM and DrugBank to obtain genes related to lung cancer.After taking the intersection,the candidate gene target of drug pair in the treatment of lung cancer could be obtained.The"herbs-compounds-targets-disease"network was bulit with Cytoscape,and the PPI network was bulit on the STRING platform while the core network nodes were screened.GO and KEGG analysis on candidate genes was implemented through Metacape platform,and a"pathways-targets"network was bulit to further screen key genes.Results:A total of 16 active compounds in salvia chinensis,18 active compounds in radix ranunculi ternati,164 candidate targets,2443 GO functions and 170 KEGG pathways was obtained.Conclusion:The effective compounds of"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer are quercetin,ursolic acid,β-sitosterol and caffeic acid.The key targets are MAPK1,AKT1,PIK3R1,RAF1 and EGFR.GO functions mainly include cytokines,oxidative stress,plasma membrane transmission,protein kinase binding and activity,apoptosis.KEGG could directly regulate pathways in cancer,non-small cells lung cancer pathway and small cell lung cancer pathway.KEGG also involves EGFR tyrosine kinase inhibitor resistance,IL-17,TNF,PI3K-AKT signaling pathway and apoptosis.This study reveals the molecular biological mechanism of"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer.It is reasoned that its potential targets affect multiple signaling pathways and ultimately resist the proliferation,differentiation,invasion,metastasis and promote apoptosis of lung cancer cells.Evidence for further experimental study is provided by this study.展开更多
该文对不同处理方式得到的两种凉粉草多糖(Mesona chinensis polysaccharides,MCP)的理化性质和体外抗氧化活性进行研究,采用同步热分析仪、紫外光谱、红外光谱、X射线衍射、扫描电镜(scanning electron microscope,SEM)等方法表征多糖...该文对不同处理方式得到的两种凉粉草多糖(Mesona chinensis polysaccharides,MCP)的理化性质和体外抗氧化活性进行研究,采用同步热分析仪、紫外光谱、红外光谱、X射线衍射、扫描电镜(scanning electron microscope,SEM)等方法表征多糖结构,从DPPH自由基清除率、ABTS+自由基清除率以及还原力方面考察多糖抗氧化活性。结果表明,在动态旋转水合半径上MCP-A溶液比MCP-B溶液黏度大;热分析结果表明,MCP-A比MCP-B具有更强的耐热性;红外光谱结果表明,两种多糖都具有多糖的特征吸收峰,MCP-A为α-糖苷键和β-糖苷键的吡喃型糖苷环骨架,MCP-B为β-糖苷键的吡喃型糖苷环骨架;X射线衍射图谱表明两种多糖主要形态以非晶体存在;SEM结果表明MCP-B比MCP-A具有更大比表面积。体外抗氧化结果显示,MCP-A清除DPPH自由基、ABTS+自由基的能力比MCP-B强,二者均有较强的还原力。研究表明,两种处理方法得多糖的理化性质和抗氧化存在明显差异,MCP-A具有更高的热稳定性和抗氧化能力,可作为可潜在的抗氧化食品成分用于食品加工。展开更多
前期研究发现石见穿多糖(polysaccharides from Salvia chinensis Benth.,PSSC)能有效抑制H22肝癌细胞在昆明小鼠体内的生长。在此基础上,本研究进一步探讨了PSSC对H22荷瘤小鼠免疫器官和免疫细胞的影响。通过在昆明小鼠右腋皮下注射H2...前期研究发现石见穿多糖(polysaccharides from Salvia chinensis Benth.,PSSC)能有效抑制H22肝癌细胞在昆明小鼠体内的生长。在此基础上,本研究进一步探讨了PSSC对H22荷瘤小鼠免疫器官和免疫细胞的影响。通过在昆明小鼠右腋皮下注射H22肝癌细胞构建荷瘤小鼠模型。采用流式细胞术、免疫组织化学染色、酶联免疫法等实验方法评价PSSC对CD4+T细胞、CD8+T细胞、自然杀伤细胞(NK细胞)抗肿瘤免疫能力的影响。结果表明PSSC可以有效增加小鼠的脾脏/胸腺指数并促进Con A/LPS刺激的脾细胞增殖。同时,PSSC剂量依赖性地增加了荷瘤小鼠外周血单核细胞、脾脏及淋巴结中CD8+T细胞比例并提高CD8+T细胞与NK细胞在肿瘤组织中的组分含量。注射PSSC后,CD4+T细胞分泌免疫促进因子IFN-γ和IL-2的量增加,而分泌免疫抑制因子IL-4和IL-10的量减少。这一系列实验研究结果表明PSSC具有显著的抗肿瘤免疫增强活性。展开更多
基金supported by the National Natural Science Foundation of China(NSFC:41801027)the National Natural Science Foundation of China(NSFC:3170043).
文摘To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.
基金National Natural Science Foundation of China(No.1673961)Beijing Natural Science Foundation Project(No.7172186)Special Training Program for Outstanding Young Scientific and Technological Talents(innovation)of Chinese Academy of Traditional Chinese Medicine(No.ZZ13-YQ-028)。
文摘Objective:To explore the molecular biological mechanism of the"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer based on network pharmacology.Methods:Searching the TCMSP database and previous literatures to screen the active compounds which resist lung cancer activity in salvia chinensis and radix ranunculi ternati.The candidate compounds were unified in the DrugBank to find the corresponding drug targets which were corrected to the standard gene names by the UniProt database.The Swiss Target Prediction platform was used to predict other targets.Searching GeneCards,OMIM and DrugBank to obtain genes related to lung cancer.After taking the intersection,the candidate gene target of drug pair in the treatment of lung cancer could be obtained.The"herbs-compounds-targets-disease"network was bulit with Cytoscape,and the PPI network was bulit on the STRING platform while the core network nodes were screened.GO and KEGG analysis on candidate genes was implemented through Metacape platform,and a"pathways-targets"network was bulit to further screen key genes.Results:A total of 16 active compounds in salvia chinensis,18 active compounds in radix ranunculi ternati,164 candidate targets,2443 GO functions and 170 KEGG pathways was obtained.Conclusion:The effective compounds of"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer are quercetin,ursolic acid,β-sitosterol and caffeic acid.The key targets are MAPK1,AKT1,PIK3R1,RAF1 and EGFR.GO functions mainly include cytokines,oxidative stress,plasma membrane transmission,protein kinase binding and activity,apoptosis.KEGG could directly regulate pathways in cancer,non-small cells lung cancer pathway and small cell lung cancer pathway.KEGG also involves EGFR tyrosine kinase inhibitor resistance,IL-17,TNF,PI3K-AKT signaling pathway and apoptosis.This study reveals the molecular biological mechanism of"salvia chinensis and radix ranunculi ternati"drug pair in the treatment of lung cancer.It is reasoned that its potential targets affect multiple signaling pathways and ultimately resist the proliferation,differentiation,invasion,metastasis and promote apoptosis of lung cancer cells.Evidence for further experimental study is provided by this study.
文摘该文对不同处理方式得到的两种凉粉草多糖(Mesona chinensis polysaccharides,MCP)的理化性质和体外抗氧化活性进行研究,采用同步热分析仪、紫外光谱、红外光谱、X射线衍射、扫描电镜(scanning electron microscope,SEM)等方法表征多糖结构,从DPPH自由基清除率、ABTS+自由基清除率以及还原力方面考察多糖抗氧化活性。结果表明,在动态旋转水合半径上MCP-A溶液比MCP-B溶液黏度大;热分析结果表明,MCP-A比MCP-B具有更强的耐热性;红外光谱结果表明,两种多糖都具有多糖的特征吸收峰,MCP-A为α-糖苷键和β-糖苷键的吡喃型糖苷环骨架,MCP-B为β-糖苷键的吡喃型糖苷环骨架;X射线衍射图谱表明两种多糖主要形态以非晶体存在;SEM结果表明MCP-B比MCP-A具有更大比表面积。体外抗氧化结果显示,MCP-A清除DPPH自由基、ABTS+自由基的能力比MCP-B强,二者均有较强的还原力。研究表明,两种处理方法得多糖的理化性质和抗氧化存在明显差异,MCP-A具有更高的热稳定性和抗氧化能力,可作为可潜在的抗氧化食品成分用于食品加工。
文摘前期研究发现石见穿多糖(polysaccharides from Salvia chinensis Benth.,PSSC)能有效抑制H22肝癌细胞在昆明小鼠体内的生长。在此基础上,本研究进一步探讨了PSSC对H22荷瘤小鼠免疫器官和免疫细胞的影响。通过在昆明小鼠右腋皮下注射H22肝癌细胞构建荷瘤小鼠模型。采用流式细胞术、免疫组织化学染色、酶联免疫法等实验方法评价PSSC对CD4+T细胞、CD8+T细胞、自然杀伤细胞(NK细胞)抗肿瘤免疫能力的影响。结果表明PSSC可以有效增加小鼠的脾脏/胸腺指数并促进Con A/LPS刺激的脾细胞增殖。同时,PSSC剂量依赖性地增加了荷瘤小鼠外周血单核细胞、脾脏及淋巴结中CD8+T细胞比例并提高CD8+T细胞与NK细胞在肿瘤组织中的组分含量。注射PSSC后,CD4+T细胞分泌免疫促进因子IFN-γ和IL-2的量增加,而分泌免疫抑制因子IL-4和IL-10的量减少。这一系列实验研究结果表明PSSC具有显著的抗肿瘤免疫增强活性。
