From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentration...Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were ： 2 ul of 10 x buffer （ Mg2＋）, 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.展开更多
In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The m...In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The method had good linearity,precision,stability and recovery.Chromatographic fingerprints were determined by HPLC-DAD using rosmarinic acid peaks as references,and 17 common peaks were selected.The similarity indexes calculated based on similarity system theory of the 10 samples were higher than 0.948,indicating good correlation among the common peaks.The chromatograms distinguished S.przewalskii from Salvia miltiorrhiza and identified the origins of S.przewalskii.These results suggested that the proposed method was suitable for S.przewalskii quality control.展开更多
The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the qu...The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method.With the application of the multipiy regression,the Free-Wilson pattern was established by the dummy(indicator)variable of the structural fragments or substituents.The results showed:(1)The diketone moiety played the fundamental role in antibacterial activities,ortho-quinones was higher active than para-quinones.(2)The single bond existed between the C15 and the C16 at D-ring led to higher activity.(3)The saturation at the A-ring led to higher activity.(4)The components adjacent to nitrogen substituents at C15 led to higher activity.(5)The hydroxylation or dehydrogenation at A-ring led to less activity.The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.展开更多
文摘From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
文摘Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
基金Supported by Project of Qinghai Science and Technology Department(2011-NF19)Fund for Middle-aged and Young Scientists of Qinghai University(2012-QYT-1)
文摘Based on single-factor experiment and L9 （34 ） orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were ： 2 ul of 10 x buffer （ Mg2＋）, 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.
基金The Program of Chinese Herbal Medicines Breeding Research of Sichuan(Grant No.2016NYZ0036-3-3)the Program of Sichuan Science and Technology Conditional Platform Construction(Grant No.2018TJPT0013)
文摘In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The method had good linearity,precision,stability and recovery.Chromatographic fingerprints were determined by HPLC-DAD using rosmarinic acid peaks as references,and 17 common peaks were selected.The similarity indexes calculated based on similarity system theory of the 10 samples were higher than 0.948,indicating good correlation among the common peaks.The chromatograms distinguished S.przewalskii from Salvia miltiorrhiza and identified the origins of S.przewalskii.These results suggested that the proposed method was suitable for S.przewalskii quality control.
基金This work was supported by a grant from the National Tackle Key Projects of Chinathe project of sciences and techniques of CAAS.
文摘The antibacierial(staphylococcus aureus)activities of 23 diterpene quinones from the Salvia przewalskii Maxim,the artificial synthesis ones and that of the metabolism in pigs were examined in an effort to study the quantitative structure activity relationship with the Free-Wilson method.With the application of the multipiy regression,the Free-Wilson pattern was established by the dummy(indicator)variable of the structural fragments or substituents.The results showed:(1)The diketone moiety played the fundamental role in antibacterial activities,ortho-quinones was higher active than para-quinones.(2)The single bond existed between the C15 and the C16 at D-ring led to higher activity.(3)The saturation at the A-ring led to higher activity.(4)The components adjacent to nitrogen substituents at C15 led to higher activity.(5)The hydroxylation or dehydrogenation at A-ring led to less activity.The research results can provide the theoretical basis for developing a new antibacterial drug from the Salvia diterpene quinones.
