Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
Based on single-factor experiment and L9 (34 ) orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentration...Based on single-factor experiment and L9 (34 ) orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were : 2 ul of 10 x buffer ( Mg2+), 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.展开更多
文摘Neo-przewaquinone A was isolated from the root of Salvia przewalskii Maxim. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.
文摘From the root of Salvia przewalskii Maxim.a new phenolic acid, przewalskinic acid A was isolated and the structure was established by the analysis of^(13)C-NMR,~1H-NMR and two-dimensional COSY experiments.
基金Supported by Project of Qinghai Science and Technology Department(2011-NF19)Fund for Middle-aged and Young Scientists of Qinghai University(2012-QYT-1)
文摘Based on single-factor experiment and L9 (34 ) orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The resuhs indicated that the optimal concentrations of various components in a 20 ill ISSR-PCR reaction system of S. przewalskii Maxim. were : 2 ul of 10 x buffer ( Mg2+), 0.4 mmol/L dNTPs, 1.0 U of Taq DNA polymerase, 0.3 umol/L ISSR primers and 30 ng of DNA template. This study laid the foun- dation for the utilization of S. przewalskii Maxim. germplasm resources.