In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture （25 μL total volume） that contained 2.0 μL ...In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture （25 μL total volume） that contained 2.0 μL 10×buffer, 0.45 mmol·L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2＋, 2 U Taq DNA polymerase, 0.30 umol·L^-2 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.展开更多
The branching system of higher plants plays a very important role in plant morphogenesis,and the number of branches can directly affect crop yield and the ornamental value of plants.It is a complicated development pro...The branching system of higher plants plays a very important role in plant morphogenesis,and the number of branches can directly affect crop yield and the ornamental value of plants.It is a complicated development process involving complex molecular mechanisms.The‘Cailinghong’variety of Salvia splendens is characterized by its great branching ability with the ability to grow into a spherical form naturally,without pinching.To gain insight into the molecular events during the branching development of S.splendens,suppressive subtractive hybridization(SSH)technology was used to screen differentially expressed genes between the erect plant type(strain 35)and the spherical plant type(‘Cailinghong’).In total,96 and 116 unigenes were annotated.Four and eight unigenes up-regulated in‘Cailinghong’and strain 35,respectively,were associated with plant hormone anabolism and signal transduction,suggesting that they participate in the branching process.One of these genes,phytoene synthase(PSY),is a precursor of the new plant hormone group strigolactones.Using the PSY fragment(192 bp)as a template,the cDNA sequence of PSY in S.splendens was cloned and named SsPSY.A relative expression analysis and transgenic test results indicated that SsPSY plays an important role in lateral branch development in‘Cailinghong’.These results provide new insight into the molecular mechanisms underlying branching in S.splendens.展开更多
文摘In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture （25 μL total volume） that contained 2.0 μL 10×buffer, 0.45 mmol·L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2＋, 2 U Taq DNA polymerase, 0.30 umol·L^-2 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.
基金the Special Project of the University in 2019-Capacity Building of Science and Technology Innovation Service-Construction of Scientific Research-Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees(2011 Collaborative Innovation Center)(Project No.CEFF-PXM2019_014207_000099)the National Natural Fund(Project No.31100509)+1 种基金Open Project of Beijing Engineering Research Center of Rural Landscape Planning and Design(KF2019065)General Project of Science and Technology Plan of Beijing Education Commission(KM202010020006).
文摘The branching system of higher plants plays a very important role in plant morphogenesis,and the number of branches can directly affect crop yield and the ornamental value of plants.It is a complicated development process involving complex molecular mechanisms.The‘Cailinghong’variety of Salvia splendens is characterized by its great branching ability with the ability to grow into a spherical form naturally,without pinching.To gain insight into the molecular events during the branching development of S.splendens,suppressive subtractive hybridization(SSH)technology was used to screen differentially expressed genes between the erect plant type(strain 35)and the spherical plant type(‘Cailinghong’).In total,96 and 116 unigenes were annotated.Four and eight unigenes up-regulated in‘Cailinghong’and strain 35,respectively,were associated with plant hormone anabolism and signal transduction,suggesting that they participate in the branching process.One of these genes,phytoene synthase(PSY),is a precursor of the new plant hormone group strigolactones.Using the PSY fragment(192 bp)as a template,the cDNA sequence of PSY in S.splendens was cloned and named SsPSY.A relative expression analysis and transgenic test results indicated that SsPSY plays an important role in lateral branch development in‘Cailinghong’.These results provide new insight into the molecular mechanisms underlying branching in S.splendens.
