Effects of complex salvia miltiorrhiza on blood coagulation, inflammation, oxidative stress and endothelial index in patients with acute pancreatitis

Objective: To investigate the effects of complex salvia miltiorrhiza on blood coagulation, inflammation, oxidative stress and endothelial index in patients with acute pancreatitis. Methods: A total of 122 patients with acute pancreatitis admitted to digestive system department in our hospital from April 2016 to November 2017 were randomly divided into control group (n=61) and observation group (n=61), the control group was treated with ulinastatin and the observation group was treated with complex salvia miltiorrhiza injection on the basis of the control group. The levels of coagulation, inflammation, oxidative stress and endothelial markers were compared between the two groups. Results: After treatment, the levels of APTT and PT in the two groups were significantly higher than those before treatment, while the levels of DD were significantly lower than those before treatment, and the levels of APTT and PT [(38.19±7.05) s,(17.60±3.68) s] in the observation group were significantly higher than those in the control group, while the levels of DD [(0.51±0.10) mg/L] in the observation group were significantly lower than those in the control group;the levels of CRP, PCT and TNF-α in the two groups were significantly lower than those before treatment, and the levels of CRP, PCT and TNF-α [(29.10±4.76) mg/L, (0.59±0.17) μg/L, (57.28±10.61) ng/L] in the observation group were significantly lower than those in the control group;the levels of SOD in the two groups were significantly higher than those before treatment, but MDA was significantly lower than that before treatment, and the level of SOD [(179.10±4.76) U/mL] in the observation group was significantly higher than that in the control group after treatment, while the level of MDA [(1.97±0.55) mmol/mL] in the observation group was significantly lower than that in the control group after treatment;the levels of NO in the two groups were significantly higher than those before treatment, while the levels of ET and vWF were significantly lower than those before treatment, and the levels of NO [(10.56±1.17) U/mL] in the observation group were significantly higher than those in the control group after treatment, while the levels of ET and vWF [(105.83±5.20) pg/mL, (102.47±16.91)%] in the observation group were significantly lower than those in the control group after treatment. Conclusions:Complex salvia miltiorrhiza injection can improve the blood coagulation function, alleviate inflammation, enhance antioxidant function and vascular endothelial function in patients with acute pancreatitis, which has clinical value of promotion.

Effect of low-dose aspirin and Danshen injection on hemorheology, vascular endothelial function and oxidative stress in pregnancy-induced hypertension

Objective:To investigate the effect of low dose aspirin and salvia miltiorrhiza injection on hemorrheology, endothelial function and oxidative stress in patients with pregnancy induced hypertension.Methods: One hundred and eighty pregnancy-induced hypertension patients treated in our hospital from April 2015 to November 2016 were selected as study subjects and randomly divided into control group and observation group with 90 cases in each group. The control group was given routine treatment. The observation group was treated with low-dose aspirin plus Salvia miltiorrhiza in the control group. After treatment, blood rheology, vascular endothelial function and oxidative stress were measured in two groups.Results: The levels of MAP and 24 h Upro in both groups were significantly lower than those before treatment. After treatment, the MAP and 24 h Upro levels in the observation group were significantly lower than those in the control group. After treatment, the level of PT was significantly higher than that before treatment, while the level of DD and Fg was significantly lower than that before treatment. Comparing the two groups after treatment showed that the PT level, and the levels of DD and Fg were significantly lower than those in the control group;After treatment, NO levels in both groups were significantly higher than those before treatment, ET-1 and ET-1/NO levels were significantly lower than before treatment, after treatment, ET-1, NO and ET The NO level in the observation group was significantly higher than that in the control group, while ET-1 and ET-1/NO levels were significantly lower than those in the control group;Compared with those before treatment, the levels of SOD in the two groups were significantly increased and MDA significantly decreased after treatment. The SOD and MDA levels in the two groups after treatment showed that the SOD level in the observation group was significantly higher than that in the control group, while MDA level was significantly lower than that of the control group.Conclusion: The combination of low-dose aspirin and Salvia miltiorrhiza injection has significant therapeutic effect on gestational hypertension, and can effectively improve the hemorheological parameters and endothelial function of patients and reduce the oxidative stress injury in patients. It is worth further clinical promotion.

Salviae miltiorrhizae ameliorates cirrhosis and portal hypertension by inhibiting nitric oxide in cirrhotic rats

OBJECTIVE: To determine the effect of Salviae miltiorrhizae on cirrhosis and portal hyperiension by inhibiting nitric oxide synthase type Ⅱ (NOSⅡ) in rats. METHODS: Real time RT-PCR was used to detect the expression of NOSII mRNA. The enzymatic activity of nitric oxide synthase and the circulating levels of nitric oxide (NO), systemic and portal hemodynamics, and quantification of cirrhosis were measured with highly sensitive methods. Traditional Chinese medicine was utilized to treat cirrhotic rats and the function of NO was evaluated. Double-blind method was applied in the experiment constantly. RESULTS: The concentration of NO increased markedly at all stages of cirrhosis, and so did the enzymatic activity of NOS, antl the iNOSmRNA expressed greatly. Meanwhile the portal-venous-pressure (PVP), portal-venous-now (PVF) increased significantly. NO, Nos and iNOSmHNA were positively correlated to the quanlity of hepatic fihrosis. Salviae Miltiorrhizae significantly inhibited NO production and inhibited the expression of iNOSmRNA. CONCLUSIONS: The increased hepatic expression of NoSIⅡ is one of the important factors causing cirrhosis and portal hypertension. Salviae Miltiorrhizae significantly ameliorates cirrhosis and portal hypertension.

Salvia fruticosa reduces intrinsic cellular and H_2O_2-induced DNA oxidation in HEK 293 cells;assessment using flow cytometry

Objective:To investigate the role of water-soluble extract of Salvia fruticosa(Creek sage)(S.fruticosa) leaves in reducing both intrinsic cellular and H_2O_2-induced DNA oxidation in cultured human embryonic kidney 293 cells.S.fruiicosa.native to the Eastern-Mediterranean basin,is widely used as a medicinal herb for treatment of various diseases.Methods:Dried leaves of 5.fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations.Each mL of the preparation contained(7.1±1.0)mg of extract.HEK-293 cells were incubated in one set with S.fruticosa extract in the presence of 0.1 mmol/L H_2O_2,and in the other set with the addition of the extract alone.The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivarization of 8-oxoguanine moieties.The fluorescence was measured using flow cytometry technique.Results:Cells incubated 3 h with 150μL extract and exposed to 0.1 mmol/L H_2O_2 showed lower intensity of fluorescence,and thus lower DNA oxidation.Moreover,cells incubated 3 h with 100μl.of the extract showed lower intensity of fluorescence,and thus lower intrinsic cellular DNA oxidation compared to control(without S.fruticosa).Conchisions:The results from this study suggest that the water-soluble extract of S.fruticosa leaves protects against both H_2O_2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.

A STUDY OF THE RADIOSENSITIVE EFFECTS ON MAMMARY CARCINOMA IN MICE BY CHINESE MEDICINE (SALVIA PLUS ASTRAGALUS) AND ASPIRIN

The effect of treatment with Chinese medicine including Salvia miltiorrhizan and Astragalus, and the nonsteroid anti-inflammatory drug aspirin alone or combining radiotherapy, was investigated in 6-8 week-old TA2 mice being inoculated mammary carcinoma. The date indicated the following conclusions: the tumor growth could be inhibited by aspirin alone (p<0.01) but Chinese medicine group was observed. Mice treated with radiotherapy together with medicine both Chinese medicine and aspirin, had a statistically significant tumor inhibiting (p<0.01) as compared to mice treated with radiotherapy alone. The function to prevent the normal tissues from radiation by these two medicine groups were observed simultaneously. In addition, blood-flow volume of microcirculation, immune function and lymphocyte micronucleus were examined, which were used to analyse potential mechanism of sensitizing enhancement for Chinese medicine and aspirin.

阿司匹林联合替格瑞洛治疗冠心病不稳定型心绞痛的疗效及对患者心室重塑、氧化应激的影响

目的 观察阿司匹林联合替格瑞洛治疗冠心病不稳定心绞痛的疗效,并评估其对患者心室重塑、氧化应激等指标的影响。方法 选取2021年10月至2023年10月期间新乡市中心医院收治的186例冠心病不稳定心绞痛患者纳入研究,采用随机数表法分为观察组和对照组,每组93例。对照组患者采用阿司匹林联合氢氯吡格雷片治疗,观察组患者采用阿司匹林联合替格瑞洛治疗,均连续治疗3个月。比较两组患者的临床疗效,以及治疗前后的心室重塑[基质金属蛋白酶-9 (MMP-9)、半乳凝素-3 (Gal-3)]、血管内皮功能[血清细胞间黏附分子-1 (ICAM-1)、血管紧张素Ⅱ(AngⅡ)、内皮素-1 (ET-1)、血流介导舒张功能(FMD)水平]和氧化应激[一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)]水平。结果 观察组患者的治疗总有效率为96.77%,明显高于对照组的86.02%,差异有统计学意义(P<0.05);观察组患者治疗后的MMP-9水平为(141.28±19.33)μg/L,明显低于对照组的(164.72±19.86)μg/L,而Gal-3水平为(6.87±0.92) ng/mL,明显高于对照组的(5.36±0.84) ng/mL,差异均有统计学意义(P<0.05);观察组患者治疗后的ICAM-1、AngⅡ和ET-1水平分别为(56.87±9.70) pg/mL、(0.70±0.14) ng/mL和(43.15±5.83) pg/mL,明显低于对照组的(81.35±10.31) pg/mL、(0.96±0.15) ng/mL和(52.37±6.37) pg/mL,而FMD水平为(14.82±1.93)%,明显高于对照组的(11.27±2.33)%,差异均有统计学意义(P<0.05);观察组患者治疗后的NO、SOD水平分别为(77.95±9.60) mol/L、(112.69±11.47) U/L,明显高于对照组的(62.49±9.37) mol/L、(107.34±10.63) U/L,而MDA水平为(12.45±2.26) mmol/L,明显低于对照组的(15.38±2.35) mmol/L,差异均有统计学意义(P<0.05)。结论 阿司匹林联合替格瑞洛治疗不稳定型冠心病疗效确切,且能促进患者的心室重塑、改善血管内皮功能及氧化应激指标。

丹参对糖尿病肾病保护作用及机制研究

糖尿病肾病(diabetes nephropathy,DN)是导致终末期肾脏病的首位致病因素,严重威胁患者生命健康。传统中医药在糖尿病肾病的治疗中具有重要潜力作用,丹参作为活血化瘀药代表,具有活血祛瘀、清心除烦之功。经研究发现,丹参含有丹酚酸、丹参酮、挥发油、多糖、含氮类化合物等多种成分,能够通过多途径多靶点改善肾小球超滤过状态和间质纤维化等病理改变,其中丹酚酸类和丹参酮类是丹参发挥糖尿病肾病保护作用的主要生物活性成分,具有降糖、调脂、抗炎、抗氧化应激、改善血流动力学、纠正肠道菌群紊乱、调节自噬水平等多种药理作用。该文参考近年来丹参相关制剂干预糖尿病肾病的临床研究和动物实验,对丹参在糖尿病肾病发生发展过程中起到的作用机制进行归纳总结,以期为临床应用丹参治疗糖尿病肾病提供更好的治疗策略和理论依据。

不同剂量人免疫球蛋白联合阿司匹林治疗小儿川崎病的效果及对血管内皮功能、氧化应激指标的影响

目的探讨不同剂量人免疫球蛋白联合阿司匹林治疗小儿川崎病的效果。方法选取2020年4月至2022年4月我科收治的60例川崎病患儿,以随机数字表法将其分为对照组和观察组,每组30例。对照组采用1.0 g/(kg·d)人免疫球蛋白联合阿司匹林治疗,观察组采用2.0 g/(kg·d)人免疫球蛋白联合阿司匹林治疗。比较两组的治疗效果。结果观察组的治疗总有效率高于对照组,差异具有统计学意义(P<0.05)。治疗后,两组的抗内皮细胞抗体(AECA)、抗中性粒细胞胞质抗体(ANCA)及血管内皮生长因子(VEGF)水平均降低,且观察组低于对照组,差异具有统计学意义(P<0.05)。治疗后,两组的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平均升高,丙二醛(MDA)水平均降低,且观察组优于对照组,差异具有统计学意义(P<0.05)。治疗后,两组的可溶性白细胞介素-2受体(sIL-2R)、脂蛋白相关磷脂酶A2(Lp-PLA2)水平均降低,且观察组低于对照组,差异具有统计学意义(P<0.05)。两组的不良反应总发生率无明显差异(P>0.05)。结论采用2.0 g/(kg·d)人免疫球蛋白联合阿司匹林治疗小儿川崎病的效果优于1.0 g/(kg·d)人免疫球蛋白,在改善血管内皮功能、氧化应激反应及免疫炎症方面更具优势,且安全性高。

临床药师对一例阿司匹林联合阿托伐他汀致药物氧化性溶血性贫血的干预

目的临床药师对1例阿司匹林联合阿托伐他汀致药物氧化性溶血性贫血的临床表现及机制进行分析,探讨溶血性贫血发生过程中的监护策略以及临床药师在临床治疗中能够发挥的作用。方法临床药师通过对实际病例的深度分析,并利用Naranjo评分关联评价,同时综合中国知网、万方数据、PubMed、Embase数据库文献资料,总结此类不良反应的特点、发病机理、潜伏期及应对措施及其效果。结果临床药师发现1例溶血性贫血患者的红细胞破坏及骨髓代偿现象,并确证双药联用风险;文献研究揭示了平均红细胞体积分布宽度(RDW)动态变化规律,结合实际病例治疗效果,证实两药联用可能影响炎症因子表达并参与药物氧化性溶血性贫血(DOHA)发病机制。结论临床药师在识别和处理阿司匹林与阿托伐他汀联用导致的罕见DOHA不良反应中起到核心作用,尤其对于用药后短期内出现、停药后迅速缓解的情况。面对这类事件,药师应指导紧急停药,并安排全面实验室检查(血常规、尿常规、肝功能及网织红细胞计数等),考虑葡萄糖-6-磷酸脱氢酶(G6PD)基因检测以便个性化制定治疗方案。同时,强化对G6PD缺乏患者的用药教育以及定期监测相关生物标志物是保障高风险患者安全用药的关键措施。

替格瑞洛联合阿司匹林在冠心病介入治疗患者中的应用效果

目的:观察替格瑞洛联合阿司匹林在冠心病介入治疗患者中的应用效果。方法:选取2019年1月至2020年12月该院收治的100例冠心病患者进行前瞻性研究,采用随机数字表法将其分为对照组和观察组各50例。两组均接受经皮冠状动脉介入术(PCI)治疗,对照组采用氯吡格雷联合阿司匹林治疗,观察组采用替格瑞洛联合阿司匹林治疗,比较两组临床疗效、治疗前后心功能指标[心输出量(CO)、每搏输出量(SV)、左心室射血分数(LVEF)]水平、血液流变学指标水平、氧化应激指标[过氧化脂质(LPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)]水平、随访3个月主要心血管不良事件(MACE)发生率。结果:观察组治疗总有效率为98.00%(49/50),高于对照组的84.00%(42/50),差异有统计学意义(P<0.05);治疗后,观察组CO、SV和LVEF水平均高于对照组,差异有统计学意义(P<0.05);治疗后,观察组全血低切黏度、全血高切黏度和血浆黏度均低于对照组,差异有统计学意义(P<0.05);治疗后,观察组MDA、LPO水平均低于对照组,SOD水平高于对照组,差异有统计学意义(P<0.05);观察组MACE发生率为6.12%(3/49),低于对照组的20.83%(10/48),差异有统计学意义(P<0.05)。结论:替格瑞洛联合阿司匹林应用于冠心病介入治疗患者可提高治疗总有效率和心功能指标水平,改善氧化应激指标水平,降低血液流变学指标水平和MACE发生率,效果优于氯吡格雷联合阿司匹林治疗。

Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats

AIM: To investigate the role of the hydrogen-rich water (HRW) in the prevention of aspirin-induced gastric mucosal injury in rats.

Clinical study of adjuvant therapy on ischemic stroke with salviae miltiorrhizae and ligustrazine

Objective: To study the effect of adjuvant therapy with salviae miltiorrhizae and ligustrazine injection on neural function injury, degree of oxidative stress reaction, chemotactic factor and adhesion molecule in patients with ischemic stroke. <br> Methods: Patients with ischemic stroke admitted in our hospital from August 2013 to October 2015 were retrospectively analyzed and divided into conventional treatment (CT) group receiving conventional treatment and traditional Chinese medicine auxiliary (TCMA) group receiving adjuvant therapy with salviae miltiorrhizae and ligustrazine injection. After the treatment of the first month and the third month, serum was collected to detect the contents of nerve injury molecules, oxidative stress parameters, chemotactic factor and adhesion molecule. <br> Results: After the 4-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40,CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group, and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher than those of CT group. After the 8-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40, CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher than those of CT group. <br> Conclusions: Adjuvant therapy with salviae miltiorrhizae and ligustrazine injection can alleviate the neural function injury, inhibit oxidative stress reaction and the generation of chemotactic factor and adhesion molecule in patients with ischemic stroke, which is an effective medicine for treating ischemic stroke.

Progress in the study of antiplatelet and antithrombotic effects of salvia polyphenolic acid for injection

Salvia polyphenolic acid for injection is a new generation of Chinese medicine injectable,which is developed by extracting and separating the water-soluble phenolic acid active ingredients of Salvia divinorum by using modern advanced technology as raw material.Pharmacological research shows that salvia polyphenolic acid has anti-inflammatory,anti-oxidative stress,neurotrophic,regenerative and protective effects on ischemic brain injury and can reduce myocardial ischemic injury.In clinical practice,it is mainly used in the treatment of stroke(mild to moderate cerebral infarction)in the recovery period of fetish blood obstruction.In recent years,it has also been gradually extended to treating acute cerebral infarction and respiratory syndrome.Since its launch,the pharmacological effects and clinical applications of Danshen polyphenolic acid for injection are reviewed to provide research clues for further essential trials and reference basis for clinical promotion and application.

Effects of salvia miltiorrhiza bge on myocardial fibrosis in hypertensive rats

Objective: To study the effects of Salvia Miltiorrhiza Bge (SMB) on myocardial fibrosis in hypertension in rats. Methods: Normal Wistar kyoto rats served as negative control (group A) . The rats with hypertension were divided into 2 groups: the hypertension group (group B) which served as the positive control and the SMB-treated hypertension group (group C). The effects of SMB on the systolic pressure, the myocardial content of aldosterone (ALD) and nitric oxide (NO) and the activity of superoxide dismutase (SOD) in the myocardium were detected. Results: The systolic pressure of the rats of group C showed no marked change after the administration of SMB and showed no difference from that of the rats of group B. The content of collagen Ⅰ and Ⅲ of the left ventricle, the collagen Ⅰ /collagen Ⅲ ratio and the content-of ALD were lower in group C than in group B and higher in group C than in group A. The content of NO and the activity of SOD in the myocardium were higher in group C than in group B and lower in group C than in group A. Conclusion: It was concluded that SMB was able to prevent or reverse the progress of myocardial fibrosis resulting from hypertension. The mechanism of this effect of SMB might be due to the fact that SMB can decrease the collagen synthesis and ALD content and increase collagen decomposition, NO content and SOD activity in the myocardium.

Effect of salvia miltiorrhiza injection adjuvant therapy on systemic inflammatory stress state and nerve injury degree of children with bacterial Encephalitis

Objective:To discuss the effect of salvia miltiorrhiza injection adjuvant therapy on systemic inflammatory stress state and nerve injury degree of children with bacterial meningitis. Methods: 68 cases of children with bacterial meningitis who were treated in our hospital from Feb. 2014 to Jan. 2017 were selected and were divided into control group (34 cases) and observation group (34 cases) based on random number table, and patients in both groups were treated with continuous treatment for 10 d. Differences of inflammatory mediator, oxidative stress index, nerve injury markers content in cerebrospinal fluid in both groups were compared. Results: Before treatment, differences of inflammatory mediator, oxidative stress index, nerve injury markers content in cerebrospinal fluid in both groups had no statistical significance. 10 d after treatment, inflammatory mediator IL-6, PCT, sVCAM-1, CRP content in cerebrospinal fluid of children in observation group was lower than that in control group;oxidative stress index MDA content in cerebrospinal fluid was lower than that in control group, and SOD content was higher than that in control group;nerve injury markers MBP, NSE, GFAP content in cerebrospinal fluid was lower than that in control group.Conclusion: salvia miltiorrhiza injection adjuvant therapy could effectively relieve the systemic inflammatory stress state and reduce the nerve damages of children with bacterial meningitis.

Clinical study on Salvia Miltiorrhiza + calcium dobesilate treatment of early retinopathy of type 2 diabetes mellitus

Objective:To study the efficacy of Salvia Miltiorrhiza+calcium dobesilate for early retinopathy of type 2 diabetes mellitus.Methods:The patients with non-proliferative diabetic retinopathy admitted to our hospital between August 2016 and February 2018 were chosen and randomly divided into the observation group receiving Salvia Miltiorrhiza+calcium dobesilate treatment and the control group receiving calcium dobesilate treatment.Before treatment and 3 months after treatment,ophthalmologic examination was conducted to measure the hemangioma volume,macular thickness and blood spot area,and serum was collected to measure the contents of angiogenesis molecules,inflammation molecules and oxidative stress molecules.Results:The hemangioma volume,macular thickness and blood spot area as well as serum hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF),erythropoietin(EPO),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),intercellular adhesion molecule-1(ICAM-1),malondialdehyde(MDA)and advanced oxidation protein product(AOPP)levels of both groups were significantly lower whereas serum pigment epithelium-derived factor(PEDF),heme oxygenase-1(HO-1),vitamin E(VitE)and total antioxidant capacity(TAC)levels were significantly higher after treatment,and the hemangioma volume,macular thickness and blood spot area as well as serum HIF-1α,VEGF,EPO,IL-1β,TNF-α,ICAM-1,MDA and AOPP levels of observation group after treatment were significantly lower than those of control group whereas serum PEDF,HO-1,VitE and TAC levels were significantly higher than those of control group.Conclusion:Salvia Miltiorrhiza+calcium dobesilate treatment of early retinopathy of type 2 diabetes mellitus is more effective than calcium dobesilate alone in improving the condition and inhibiting the angiogenesis,inflammation and oxidative stress.

丹参对野百合碱诱导肺动脉高压大鼠肺动脉压及氧化应激的影响

目的探讨丹参对肺动脉高压大鼠肺动脉压的影响及可能作用机制。方法从24只健康雄性SD大鼠中随机选取6只作为对照组,其余大鼠采用单次腹腔注射野百合碱诱导14 d制备肺动脉高压模型。将造模成功的大鼠随机分为模型组、丹参高剂量组、丹参低剂量组,每组6只。丹参高、低剂量组分别给予1.17 g/mL、0.585 g/mL丹参溶液1 mL/100 g灌胃,对照组及模型组给予空白溶剂蒸馏水1 mL/100 g灌胃,均每日1次。灌胃14 d后,经右心导管介入测定大鼠右心室收缩压(RVSP)及平均肺动脉压(mPAP),心脏称重并计算右心室肥厚指数(RVHI),HE染色观察肺小动脉病理形态及肺动脉管壁厚度和管壁面积系数,ELISA法测定血清超氧化物歧化酶(SOD)及活性氧簇(ROS)含量。结果与对照组比较,模型组大鼠RVSP、mPAP及RVHI均明显升高(P均<0.05);HE染色显示肺小动脉内膜破坏,管壁增厚,管腔有效面积明显减小,血管壁厚度系数和血管壁面积系数明显增高(P均<0.05);血清SOD含量明显降低(P<0.05),血清ROS含量明显升高(P<0.05)。与模型组比较,丹参高、低剂量组大鼠RVSP、mPAP及RVHI均明显降低(P均<0.05);HE染色显示肺血管管壁厚度和管腔狭窄程度明显改善,血管壁厚度系数和血管壁面积系数明显降低(P均<0.05);血清SOD含量明显升高(P均<0.05),血清ROS含量明显降低(P均<0.05)。结论丹参具有改善野百合碱诱导大鼠肺动脉高压作用,其机制可能与减轻肺组织氧化应激损伤、调节氧化/抗氧化系统平衡相关。

川芎-丹参配伍治疗脊髓损伤的网络药理学研究与验证

目的:基于网络药理学,预测川芎-丹参配伍治疗脊髓损伤的作用机制。方法:利用网络药理学工具,获得川芎-丹参治疗脊髓损伤的有效成分、核心靶点及信号通路。采用改良Allen重物打击法构建脊髓损伤模型,采用酶联免疫吸附试验(ELISA)测定各组大鼠活性氧(ROS)、丙二醛(MDA)水平;采用蛋白质印迹法检测各组大鼠脊髓组织中磷脂酰肌醇3激酶(PI3K)、p-PI3K、蛋白激酶B(AKT)、p-AKT蛋白表达。结果:化合物-靶点网络包含154个化合物和484个相应靶点;基因本体(GO)功能富集分析得447个条目;京都基因和基因组百科全书(KEGG)通路富集分析得到126条信号通路。动物实验结果表明,与假手术组比较,模型组ROS、MDA水平显著增高(P<0.01);p-PI3K、p-AKT蛋白表达显著下降(P<0.01);与模型组比较,中药高剂量组ROS、MDA水平显著下降(P<0.01);p-PI3K、p-AKT蛋白表达显著增高(P<0.01)。结论:川芎-丹参联合治疗脊髓损伤具有多成分、多靶点、多通路的优势,其作用机制可能与调控PI3K/AKT信号通路有关。

Aspirin and pravastatin reduce lectin-like oxidized low density lipoprotein receptor-1 expression, adhesion molecules and oxidative stress in human coronary artery endothelial cells

Background Oxidative stress and inflammation are important steps in the pathogenesis of atherosclerosis. We postulated that therapeutic concentrations of aspirin and pravastatin, especially in combination, may suppress oxidative stress and inflammation in endothelial cells, and this concept was examined in human coronary artery endothelial cells （HCAECs）. Methods Human coronary artery endothelial cells were cultured and treated with oxidized-low density lipoprotein （ox-LDL, 60 ug/ml for 24 hours） alone, or pre-treated with aspirin （1, 2 or 5 mmol/L）, pravastatin （1, 5 or 10 umol/L） or their combination （1 mmol/L aspirin and 5umol/L pravastatin）, followed by ox-LDL treatment. After respective treatment, superoxide anion production, p38 mitogen activated protein kinase and transcription factor NF-KB activation, protein expression of lectin-like ox-LDL receptor-1 （LOX-1） and adhesion molecules, and monocyte adhesion were measured. Results Ox-LDL treatment greatly elicited its receptor LOX-1 expression, superoxide anion production and inflammatory response, which were minimally affected by low concentration of aspirin （1 mmol/L） or pravastatin （5umol/L）, but were markedly decreased by their combination. Activation of p38 mitogen activated protein kinase and NF-KB, the expression of intercellular adhesion molecule-1 and monocyte chemotactic protein-I, which were only mildly affected by aspirin or pravastatin alone, were significantly attenuated by their combination. As a consequence, monocyte adhesion to endothelial cells was markedly attenuated by the combination of the two agents. Well-known anti-oxidants a-tocopherol and y-tocopherol had similar inhibitory effects on ox-LDL-mediated oxidative stress and LOX-1 expression as well as monocyte adhesion as did the combination of aspirin and pravastatin. Conclusions These studies point to a positive interaction between aspirin and pravastatin with regard to endothelial bioloqy. Anti-oxidant and subsequent anti-inflammatory effect may be one of the potential underling mechanisms.