BACKGROUND： Studies have shown that both salviae miltiorrhizae and ligustrazine can promote protein expression of nerve growth factor （NGF） and regeneration of peripheral nerve. OBJECTIVE： To verify the effect of ...BACKGROUND： Studies have shown that both salviae miltiorrhizae and ligustrazine can promote protein expression of nerve growth factor （NGF） and regeneration of peripheral nerve. OBJECTIVE： To verify the effect of salviae miltiorrhizae and ligustrazine hydrochloride injection on axonal regeneration and NGF protein expression in a rat model of sciatic nerve injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Traditional Chinese Medicine and the Institute of Bioengineering of Jinan University from July to December 2008. MATERIALS： Salviae miltiorrhizae and ligustrazine hydrochloride injection （containing 20 mg salviae miJtiorrhizae and 100 mg ligustrazine per 100 mL injection） was provided by Guizhou Baite Pharmaceutical, China; salviae miltiorrhizae and ligustrazine decoctions （containing 1 g raw drug per 1 mL decoction） were provided by Guangzhou Baiyunshan Factory for Traditional Chinese Medicine, China; rabbit-anti-rat NGF monoclonal antibody was provided by Beijing Biosynthesis Biotechnology, China. METHODS： A total of 80 healthy, male, Sprague Dawley rats were used to establish a sciatic nerve injury model via neurotomy, and were then randomly assigned to 4 groups： salviae miltiorrhizae and ligustrazine hydrochloride injection group （intraperitoneal injection of 35 mL/kg per day salviae miltiorrhizae and ligustrazine hydrochloride injection）, saIviae miltiorrhizae group （intragastric peffusion of 2 mL salviae miltiorrhizae）, ligustrazine group （intragastric peffusion of 2 mL ligustrazine）, and model group （intraperitoneal injection of 35 mL/kg per day saline）, with 20 rats in each group. Thereafter, rats in each group were then divided into 4 subgroups according to varying time points of 1, 2, 4, and 8 weeks post-surgery, with 5 rats in each subgroup. MAIN OUTCOME MEASURES： Axons were quantified using chromotrope 2R-brilliant green and silver staining combined with image analysis to calculate the axonal regeneration rate; NGF expression was detected using immunohistochemistry and Western blot analysis; toe interspace was measured by behavior at 4 and 8 weeks. RESULTS： With increasing time after sciatic nerve expression, and toe interspace gradually increased njury, the axonal regeneration rate, NGF protein At 4 and 8 weeks post-surgery, axonal regeneration rate and NGF protein expression were significantly increased in the injured tissue of the salviae miltiorrhizae and ligustrazine hydrochloride injection, salviae miltiorrhizae, and ligustrazine groups, compared with the model group （P 〈 0.05 or P 〈 0.01）, and toe interspace was remarkably enlarged （P 〈 0.05 or P 〈 0.01）, especially in the salviae miltiorrhizae and ligustrazine hydrochloride injection group. CONCLUSION： Salviae miltiorrhizae and ligustrazine hydrochloride injection promoted axonal regeneration and NGF protein expression in the injured sciatic nerve, and also enhanced neurofunctional recovery. Its effect was superior to salviae miltiorrhizae or ligustrazine alone.展开更多
Objective:To study the effect of salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection on serum BNP, Hcy, MMP-2, S100B protein and hemorheology in patients with acute...Objective:To study the effect of salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection on serum BNP, Hcy, MMP-2, S100B protein and hemorheology in patients with acute cerebral watershed infarction.Methods:A total of 90 patientswith acute cerebral watershed infarction in our hospital from August 2014 to December 2016 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with hydroxyethyl starch injection, the treatment group was treated withsalvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection, and both the two groups were treated for 2 weeks. The serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before and after treatments were compared.Results:There were no significantly differences of the serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before treatment. The serum BNP, Hcy, MMP-2, S100B proteinlevels of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. The PV, Lr, Mr, Hr and RE of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group.Conclusion:Salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injectioncan significantlyimprovetheneurological function and hemorheology, reduce inflammation of the patients with acute cerebral watershed infarction, and it was worthy clinical application.展开更多
Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection（SML） on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2...Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection（SML） on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2（NDRG2, a tumor suppressor gene）. Methods: HSCs from the LX-2 cell line were cultured in vitro. The proliferative state of different initial LX-2 cell numbers was measured using a 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） colorimetric assay. LX-2 cells were plated in 96-well plates at an approximate density of 2.50×10;cells/mL and cultured for 24 h followed by the application of different concentrations of SML（1, 2, 4 and 8 μL/mL）. Cell proliferation was measured using the MTT assay at 24 and 48 h. Apoptosis was detected by flow cytometry at 24 h. LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer. The levels of NDRG2 and β-catenin were measured by Western blot. Results: With the exception of the 1 and 2 μL/mL concentrations, 4 and 8 μL/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h（P<0.05）. With the exception of the 1 and 2 μL/mL concentrations, the NDRG2 expression level was greatly increased in a concentration-dependent manner. However, the level of β-catenin was unaffected. Conclusion: SML inhibit LX-2 cell proliferation in a concentration-dependent manner, and the mechanism may be associated with NDRG2 over-expression.展开更多
目的用循证医学方法 Meta分析评价丹参川芎嗪注射液治疗不稳定型心绞痛的临床效果及安全性。方法计算机检索及手工检索相关文献,由两名研究者按纳入与排除标准独立选择试验、交叉核对、评价质量、提取数据,并采用Cochrane协作网专用软件...目的用循证医学方法 Meta分析评价丹参川芎嗪注射液治疗不稳定型心绞痛的临床效果及安全性。方法计算机检索及手工检索相关文献,由两名研究者按纳入与排除标准独立选择试验、交叉核对、评价质量、提取数据,并采用Cochrane协作网专用软件Rev Man 5.0软件进行分析。结果经筛选最终纳入12篇关于丹参川芎嗪注射液治疗不稳定型心绞痛的随机对照试验,与对照组比较,丹参川芎嗪注射液能提高不稳定型心绞痛患者临床疗效、心电图、血脂等指标并具有统计学意义,且不良反应发生率低。结论丹参川芎嗪注射液联合常规西医治疗不稳定心绞痛更具优势,但结论尚需更多高质量的多中心、随机、双盲、对照的临床试验进一步证实。展开更多
目的评价丹参川芎嗪注射液治疗脑梗死的临床疗效和安全性。方法计算机检索国内外公开发表的以丹参川芎嗪注射液为干预措施治疗脑梗死的随机对照试验,采用Jadad评分法进行质量评价,运用Rev Man 5.0.0软件进行Meta分析。结果 13项研究经M...目的评价丹参川芎嗪注射液治疗脑梗死的临床疗效和安全性。方法计算机检索国内外公开发表的以丹参川芎嗪注射液为干预措施治疗脑梗死的随机对照试验,采用Jadad评分法进行质量评价,运用Rev Man 5.0.0软件进行Meta分析。结果 13项研究经Meta分析结果显示,丹参川芎嗪注射液治疗组在痊愈率、有效率方面均优于对照组。结论丹参川芎嗪注射液治疗脑梗死有较好疗效,且不良反应发生率低,但由于现有的研究质量较差,尚需设计严格的多中心、大样本的随机对照试验予以进一步证实。展开更多
目的研究丹参川芎嗪注射液对大鼠脑基底动脉的扩张及其机制。方法利用小血管张力测量系统研究丹参川芎嗪注射液对经高K^+、苯肾上腺素(PE)和血栓素受体激动剂(U46619)预收缩的血管环的扩张作用,并研究在不含钙的缓冲液中丹参川芎嗪注射...目的研究丹参川芎嗪注射液对大鼠脑基底动脉的扩张及其机制。方法利用小血管张力测量系统研究丹参川芎嗪注射液对经高K^+、苯肾上腺素(PE)和血栓素受体激动剂(U46619)预收缩的血管环的扩张作用,并研究在不含钙的缓冲液中丹参川芎嗪注射液对PE、氯化钙(CaCl_2)及咖啡因收缩血管的影响。利用激光共聚焦显微镜观察丹参川芎嗪注射液对大鼠肠系膜血管平滑肌钙离子浓度的影响。结果 15~80 m L·L^(-1)丹参川芎嗪注射液可以浓度依赖性地降低经高K^+、PE和U46619预收缩的大鼠脑基底动脉;在不含钙的缓冲液中,丹参川芎嗪注射液(15,45,80 m L·L^(-1))可以抑制CaCl_2诱导的血管收缩,对PE及咖啡因诱导的血管收缩无影响;丹参川芎嗪注射液(45,60,80 m L·L^(-1))对高K^+诱导的大鼠肠系膜血管环收缩及血管壁荧光增强有不同程度的抑制作用。结论丹参川芎嗪注射液可以浓度依赖性地舒张大鼠脑基底动脉,抑制血管平滑肌钙离子通道是主要作用机制之一,主要表现为抑制细胞外的钙离子进入。展开更多
基金Supported by: the Natural Science Foundation of Guangdong province, No. 5300544High-Tech Research and Development Program of Guangdong Province, No. 2009B030801238+3 种基金2006B35602009the Grants from Guangdong Province Administration of Traditional Chinese Medicine, No. 2008092 1060114the Science and Technology Foundation of Guangzhou,No.2009Z1-E091
文摘BACKGROUND： Studies have shown that both salviae miltiorrhizae and ligustrazine can promote protein expression of nerve growth factor （NGF） and regeneration of peripheral nerve. OBJECTIVE： To verify the effect of salviae miltiorrhizae and ligustrazine hydrochloride injection on axonal regeneration and NGF protein expression in a rat model of sciatic nerve injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Traditional Chinese Medicine and the Institute of Bioengineering of Jinan University from July to December 2008. MATERIALS： Salviae miltiorrhizae and ligustrazine hydrochloride injection （containing 20 mg salviae miJtiorrhizae and 100 mg ligustrazine per 100 mL injection） was provided by Guizhou Baite Pharmaceutical, China; salviae miltiorrhizae and ligustrazine decoctions （containing 1 g raw drug per 1 mL decoction） were provided by Guangzhou Baiyunshan Factory for Traditional Chinese Medicine, China; rabbit-anti-rat NGF monoclonal antibody was provided by Beijing Biosynthesis Biotechnology, China. METHODS： A total of 80 healthy, male, Sprague Dawley rats were used to establish a sciatic nerve injury model via neurotomy, and were then randomly assigned to 4 groups： salviae miltiorrhizae and ligustrazine hydrochloride injection group （intraperitoneal injection of 35 mL/kg per day salviae miltiorrhizae and ligustrazine hydrochloride injection）, saIviae miltiorrhizae group （intragastric peffusion of 2 mL salviae miltiorrhizae）, ligustrazine group （intragastric peffusion of 2 mL ligustrazine）, and model group （intraperitoneal injection of 35 mL/kg per day saline）, with 20 rats in each group. Thereafter, rats in each group were then divided into 4 subgroups according to varying time points of 1, 2, 4, and 8 weeks post-surgery, with 5 rats in each subgroup. MAIN OUTCOME MEASURES： Axons were quantified using chromotrope 2R-brilliant green and silver staining combined with image analysis to calculate the axonal regeneration rate; NGF expression was detected using immunohistochemistry and Western blot analysis; toe interspace was measured by behavior at 4 and 8 weeks. RESULTS： With increasing time after sciatic nerve expression, and toe interspace gradually increased njury, the axonal regeneration rate, NGF protein At 4 and 8 weeks post-surgery, axonal regeneration rate and NGF protein expression were significantly increased in the injured tissue of the salviae miltiorrhizae and ligustrazine hydrochloride injection, salviae miltiorrhizae, and ligustrazine groups, compared with the model group （P 〈 0.05 or P 〈 0.01）, and toe interspace was remarkably enlarged （P 〈 0.05 or P 〈 0.01）, especially in the salviae miltiorrhizae and ligustrazine hydrochloride injection group. CONCLUSION： Salviae miltiorrhizae and ligustrazine hydrochloride injection promoted axonal regeneration and NGF protein expression in the injured sciatic nerve, and also enhanced neurofunctional recovery. Its effect was superior to salviae miltiorrhizae or ligustrazine alone.
基金Hubei Natural Science Foundation Project Plan 2015(2015-cEV129).
文摘Objective:To study the effect of salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection on serum BNP, Hcy, MMP-2, S100B protein and hemorheology in patients with acute cerebral watershed infarction.Methods:A total of 90 patientswith acute cerebral watershed infarction in our hospital from August 2014 to December 2016 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with hydroxyethyl starch injection, the treatment group was treated withsalvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection, and both the two groups were treated for 2 weeks. The serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before and after treatments were compared.Results:There were no significantly differences of the serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before treatment. The serum BNP, Hcy, MMP-2, S100B proteinlevels of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. The PV, Lr, Mr, Hr and RE of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group.Conclusion:Salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injectioncan significantlyimprovetheneurological function and hemorheology, reduce inflammation of the patients with acute cerebral watershed infarction, and it was worthy clinical application.
基金Supported by the National Natural Science Foundation of China(No.81272490 and 81100764)
文摘Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection（SML） on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2（NDRG2, a tumor suppressor gene）. Methods: HSCs from the LX-2 cell line were cultured in vitro. The proliferative state of different initial LX-2 cell numbers was measured using a 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） colorimetric assay. LX-2 cells were plated in 96-well plates at an approximate density of 2.50×10;cells/mL and cultured for 24 h followed by the application of different concentrations of SML（1, 2, 4 and 8 μL/mL）. Cell proliferation was measured using the MTT assay at 24 and 48 h. Apoptosis was detected by flow cytometry at 24 h. LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer. The levels of NDRG2 and β-catenin were measured by Western blot. Results: With the exception of the 1 and 2 μL/mL concentrations, 4 and 8 μL/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h（P<0.05）. With the exception of the 1 and 2 μL/mL concentrations, the NDRG2 expression level was greatly increased in a concentration-dependent manner. However, the level of β-catenin was unaffected. Conclusion: SML inhibit LX-2 cell proliferation in a concentration-dependent manner, and the mechanism may be associated with NDRG2 over-expression.
文摘目的用循证医学方法 Meta分析评价丹参川芎嗪注射液治疗不稳定型心绞痛的临床效果及安全性。方法计算机检索及手工检索相关文献,由两名研究者按纳入与排除标准独立选择试验、交叉核对、评价质量、提取数据,并采用Cochrane协作网专用软件Rev Man 5.0软件进行分析。结果经筛选最终纳入12篇关于丹参川芎嗪注射液治疗不稳定型心绞痛的随机对照试验,与对照组比较,丹参川芎嗪注射液能提高不稳定型心绞痛患者临床疗效、心电图、血脂等指标并具有统计学意义,且不良反应发生率低。结论丹参川芎嗪注射液联合常规西医治疗不稳定心绞痛更具优势,但结论尚需更多高质量的多中心、随机、双盲、对照的临床试验进一步证实。
文摘目的评价丹参川芎嗪注射液治疗脑梗死的临床疗效和安全性。方法计算机检索国内外公开发表的以丹参川芎嗪注射液为干预措施治疗脑梗死的随机对照试验,采用Jadad评分法进行质量评价,运用Rev Man 5.0.0软件进行Meta分析。结果 13项研究经Meta分析结果显示,丹参川芎嗪注射液治疗组在痊愈率、有效率方面均优于对照组。结论丹参川芎嗪注射液治疗脑梗死有较好疗效,且不良反应发生率低,但由于现有的研究质量较差,尚需设计严格的多中心、大样本的随机对照试验予以进一步证实。
文摘目的研究丹参川芎嗪注射液对大鼠脑基底动脉的扩张及其机制。方法利用小血管张力测量系统研究丹参川芎嗪注射液对经高K^+、苯肾上腺素(PE)和血栓素受体激动剂(U46619)预收缩的血管环的扩张作用,并研究在不含钙的缓冲液中丹参川芎嗪注射液对PE、氯化钙(CaCl_2)及咖啡因收缩血管的影响。利用激光共聚焦显微镜观察丹参川芎嗪注射液对大鼠肠系膜血管平滑肌钙离子浓度的影响。结果 15~80 m L·L^(-1)丹参川芎嗪注射液可以浓度依赖性地降低经高K^+、PE和U46619预收缩的大鼠脑基底动脉;在不含钙的缓冲液中,丹参川芎嗪注射液(15,45,80 m L·L^(-1))可以抑制CaCl_2诱导的血管收缩,对PE及咖啡因诱导的血管收缩无影响;丹参川芎嗪注射液(45,60,80 m L·L^(-1))对高K^+诱导的大鼠肠系膜血管环收缩及血管壁荧光增强有不同程度的抑制作用。结论丹参川芎嗪注射液可以浓度依赖性地舒张大鼠脑基底动脉,抑制血管平滑肌钙离子通道是主要作用机制之一,主要表现为抑制细胞外的钙离子进入。
