Post-stroke depression (PSD) is one of the most common affective disorders after acute cerebrovascular injury. It significantly increases the mortality, disability rate and recurrence rate of stroke, which places a he...Post-stroke depression (PSD) is one of the most common affective disorders after acute cerebrovascular injury. It significantly increases the mortality, disability rate and recurrence rate of stroke, which places a heavy burden on families and society. The etiology of PSD is not yet clear, involving a series of complex mechanisms. Inflammatory response, apoptosis, neurotrophic factors, neurotransmitters and many others may play important roles, and there is no effective treatment. Salvianolic acid for injection (SAFI) has the functions of anti-inflammatory, anti-oxidative stress, anti-apoptotic and can improve depressive behavior. It is expected to become a new antidepressant for the treatment of PSD.展开更多
目的建立一标多测法(quantitative analysis of multi-components with single-marker,QAMS)测定注射用丹参多酚酸中5种成分的含量。方法采用HPLC法,Agilent C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-体积分数0.05%磷酸水溶液为流动...目的建立一标多测法(quantitative analysis of multi-components with single-marker,QAMS)测定注射用丹参多酚酸中5种成分的含量。方法采用HPLC法,Agilent C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-体积分数0.05%磷酸水溶液为流动相等度洗脱,流速为1.0 mL·min^(-1),检测波长为280 nm,柱温为25℃。以丹酚酸B为参照物,建立其与丹酚酸D、迷迭香酸、紫草酸、丹酚酸Y的相对校正因子,利用一标多测法计算5种成分的含量,同时与外标法测定结果进行对比,考察一标多测法的可行性和适用性。结果在一定的线性范围内,一标多测法的相对校正因子重复性好,两种方法计算得到的3批样品中5种成分的含量无明显差异。结论HPLC结合一标多测的方法,可为注射用丹参多酚酸的质量控制提供参考。展开更多
目的:建立用超高效液相色谱(UPLC)同时测定丹参注射剂中丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸 B 和丹酚酸A 6个主要化学成分的方法。方法:采用 UPLC 色谱系统,BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);流动相为1%甲酸-乙腈...目的:建立用超高效液相色谱(UPLC)同时测定丹参注射剂中丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸 B 和丹酚酸A 6个主要化学成分的方法。方法:采用 UPLC 色谱系统,BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);流动相为1%甲酸-乙腈梯度洗脱(乙腈:0 min 为2%,2.2 min 为4%,2.3 min 为8%,10 min 为18%,10.2 min 为42%,15 min 为54%,16 min 为80%,17 min 为80%);流速为0.6 mL·min^(-1);检测波长为280 nm。结果:本方法可在17 min 内完成1次色谱分析,各主要成分色谱峰之间有良好的分离度,6个成分的浓度和各自峰面积之间有着良好的线性关系,精密度、重复性及加样回收率的 RSD均小于1.0%。结论:本方法快捷、准确,重复性好,能同时测定丹参注射剂中6个主要化学成分,可较全面地控制丹参注射剂这些化学成分的含量。展开更多
基金Natural Science Foundation of Shanxi Provinc(201801D121219)Shanxi Provincial Health and Family Planning Commission project(2017065)
文摘Post-stroke depression (PSD) is one of the most common affective disorders after acute cerebrovascular injury. It significantly increases the mortality, disability rate and recurrence rate of stroke, which places a heavy burden on families and society. The etiology of PSD is not yet clear, involving a series of complex mechanisms. Inflammatory response, apoptosis, neurotrophic factors, neurotransmitters and many others may play important roles, and there is no effective treatment. Salvianolic acid for injection (SAFI) has the functions of anti-inflammatory, anti-oxidative stress, anti-apoptotic and can improve depressive behavior. It is expected to become a new antidepressant for the treatment of PSD.
文摘目的:建立用超高效液相色谱(UPLC)同时测定丹参注射剂中丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸 B 和丹酚酸A 6个主要化学成分的方法。方法:采用 UPLC 色谱系统,BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);流动相为1%甲酸-乙腈梯度洗脱(乙腈:0 min 为2%,2.2 min 为4%,2.3 min 为8%,10 min 为18%,10.2 min 为42%,15 min 为54%,16 min 为80%,17 min 为80%);流速为0.6 mL·min^(-1);检测波长为280 nm。结果:本方法可在17 min 内完成1次色谱分析,各主要成分色谱峰之间有良好的分离度,6个成分的浓度和各自峰面积之间有着良好的线性关系,精密度、重复性及加样回收率的 RSD均小于1.0%。结论:本方法快捷、准确,重复性好,能同时测定丹参注射剂中6个主要化学成分,可较全面地控制丹参注射剂这些化学成分的含量。
