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Morphological Development of Sambong (Blumea balsamifera (L.) DC.) Leaf Studied by Frozen Section and Thin Section
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作者 Liwei Liu Xiaolu Chen +1 位作者 Quan Yang Yuxin Pang 《Journal of Biosciences and Medicines》 2016年第4期10-13,共4页
The sambong (Blumea balsamifera (L.) DC.) leaf was investigated by applying frozen section and thin section technology, and observed through optical microscope. The results showed that the sambong leaf was firstly ori... The sambong (Blumea balsamifera (L.) DC.) leaf was investigated by applying frozen section and thin section technology, and observed through optical microscope. The results showed that the sambong leaf was firstly originated nearby the apical bud at the stem tip. The first leaf grows fast, that the mitosis happens frequently and the cytoplasm manufacture rapidly. The cells in the first few leaves have not yet differentiated into the spongy parenchyma and palisade parenchyma. In the mature leaf, the spongy parenchyma and palisade parenchyma were fully developed. The epidermal hairs were firstly developed on the abaxial side of the first leaf. 展开更多
关键词 sambong Blumea balsamifera Ainaxiang Nalong Morphological Development Thin Section
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Microscopic Structural Comparison between Epidermal Trichomes in Blumea balsamifera (L.) DC. and Blumea laciniata (Roxb.) DC
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作者 Xiaolu Chen Yingbo Zhang +1 位作者 Yuxin Pang Qian Jiang 《Journal of Biosciences and Medicines》 2016年第12期70-75,共6页
Immature leaves and stems, which were about one to three centimeters nearby the stem tips, of Blumea balsamifera (L.) DC. (BB) and Blumea laciniata (Roxb.) DC. (BL) were cut into sections under ?18?C with a frozen sec... Immature leaves and stems, which were about one to three centimeters nearby the stem tips, of Blumea balsamifera (L.) DC. (BB) and Blumea laciniata (Roxb.) DC. (BL) were cut into sections under ?18?C with a frozen section machine, and observed under an optical microscope. Results show that the most significant difference is that the BB has only one kind of glandular trichomes, while the BL has two. The glandular trichomes found on BB terms were all short glandular hairs (SGH), which were not longer than 100 μm. On BL stems, besides the SGH, the long glandular hairs (LGH), which were longer than 200 μm, were also found. By the factors pointed out in present study, the BB and BL and be distinguished from each other. 展开更多
关键词 Blumea balsamifera Blumea laciniata sambong Ainaxiang Glandular Hair
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利用冰冻切片法观察艾纳香叶显微结构 被引量:5
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作者 陈晓鹭 刘立伟 +1 位作者 杨全 庞玉新 《热带作物学报》 CSCD 北大核心 2016年第6期1086-1091,共6页
应用冰冻切片法观察艾纳香成熟叶的显微结构。结果显示,优化的冰冻切片方法为:取新鲜叶片,不经预处理,直接切取4 mm×4 mm小块,用冷凝胶包埋,冷凝箱温度和样品夹温度-18°C,冷凝时间10 min,修片后取出并置于室温20℃条件下回温... 应用冰冻切片法观察艾纳香成熟叶的显微结构。结果显示,优化的冰冻切片方法为:取新鲜叶片,不经预处理,直接切取4 mm×4 mm小块,用冷凝胶包埋,冷凝箱温度和样品夹温度-18°C,冷凝时间10 min,修片后取出并置于室温20℃条件下回温30 s,移回样品夹后30 s内切片,切片厚度20μm,用洁净载玻片吸片,于光镜下观察。成熟艾纳香叶为异面叶,厚度约110μm;栅栏组织和海绵组织分化明显,栅栏组织由1层排列紧密的柱状细胞组成,海绵组织有发达的细胞间隙,两者之比为0.82~1.22;叶中脉发达,主脉散生,外韧维管束6~10个,由厚壁细胞组成维管束鞘,导管为螺纹;叶上、下表皮均有大量非腺毛和腺毛分布,叶表皮的腺毛为双列多细胞组成的头状腺毛。本研究结果确定了利用冰冻切片研究艾纳香叶显微结构简便可行,为艾纳香属植物分类鉴定、品种整理等提供参考,并为进一步研究艾纳香的结构和发育积累经验。 展开更多
关键词 艾纳香 娜龙 腺毛 显微结构 冰冻切片
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