Effects of different media,pH values,temperatures,carbon and nitrogen sources on mycelial growth and sporulation of root rot pathogen on Sambucus chinensis was determined in the paper.The results showed that root rot ...Effects of different media,pH values,temperatures,carbon and nitrogen sources on mycelial growth and sporulation of root rot pathogen on Sambucus chinensis was determined in the paper.The results showed that root rot pathogen on S.chinensis was identified as Fusariumsp.The mycelial growth and sporulation of Fusariumsp.in PSA medium was the best;the optimal temperature for mycelial growth and sporulation was from 25℃ to 30℃ and the optimal temperature was 28℃;the suitable pH values for mycelial growth and sporulation was from 5 to 7,and the optimum pH value was 6;the absence or presence of carbon sources had significant effect on growth of Fusariumsp.;sucrose was the best carbon source utilized in mycelial growth,while lactose was the worst; KNO3 was the best nitrogen source utilized in mycelial growth,while（NH4） 2 SO4 was the worst one.展开更多
[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtai...[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.展开更多
Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and pre...Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and preventing phlegm form forming and stopping coughing. In this paper,the research on the herbal medicine,medicinal identification,chemical composition and extraction process of some chemical components,trace elements,clinical application and pharmacological action,antibacterial effect were reviewed. The existing problems in the current research were analyzed,and its future research direction were prospected,with an attempt to provide reference for its future research and development and utilization.展开更多
[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identific...[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identification,character identification,powder identification and paraffin section identification were adopted in the study. [Results]The characteristics of Yi Crude herb of Pholidota chinensis Lindl. were obvious in the original plant,Crude herb and microscopic characteristics. [Conclusions]This paper provided reference for developing quality standards of Yi Crude herb Pholidota chinensis Lindl. and original plant identification,and provided the basis for further research.展开更多
从陆英Sam bucus chinensisL ind l.分离并鉴定了5个化合物分别为:β-谷甾醇(Ⅰ)、齐墩果酸(Ⅱ)、熊果酸(Ⅲ)、山柰酚-3-O-β-D-(6-O-乙酰基-葡萄吡喃糖)-7-O-β-D-葡萄吡喃糖苷(Ⅳ)和山柰酚-3-O-β-D-葡萄吡喃糖-7-O-β-D-葡萄吡喃糖苷...从陆英Sam bucus chinensisL ind l.分离并鉴定了5个化合物分别为:β-谷甾醇(Ⅰ)、齐墩果酸(Ⅱ)、熊果酸(Ⅲ)、山柰酚-3-O-β-D-(6-O-乙酰基-葡萄吡喃糖)-7-O-β-D-葡萄吡喃糖苷(Ⅳ)和山柰酚-3-O-β-D-葡萄吡喃糖-7-O-β-D-葡萄吡喃糖苷(Ⅴ),化合物Ⅳ和Ⅴ为首次从陆英中分离得到。展开更多
文摘Effects of different media,pH values,temperatures,carbon and nitrogen sources on mycelial growth and sporulation of root rot pathogen on Sambucus chinensis was determined in the paper.The results showed that root rot pathogen on S.chinensis was identified as Fusariumsp.The mycelial growth and sporulation of Fusariumsp.in PSA medium was the best;the optimal temperature for mycelial growth and sporulation was from 25℃ to 30℃ and the optimal temperature was 28℃;the suitable pH values for mycelial growth and sporulation was from 5 to 7,and the optimum pH value was 6;the absence or presence of carbon sources had significant effect on growth of Fusariumsp.;sucrose was the best carbon source utilized in mycelial growth,while lactose was the worst; KNO3 was the best nitrogen source utilized in mycelial growth,while（NH4） 2 SO4 was the worst one.
基金Supported by Guangxi Science and Technology Base and Talent Project(GK AD21238031)Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GKJZ[2014]32)+8 种基金Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine(GJKY[2013]20)Guangxi Engineering Research Center of Ethnic Medicine Resources and Application(Guifa Gai High Technology Letter[2020]No.2605)The Eighth Batch of Guangxi Specially-employed Expert Projects(GRCTZ[2019]13)Youth Fund Project of Guangxi University of Chinese Medicine(2019QN012)Guangxi Key Research and Development Project(GK AB21196016)National Natural Science Foundation of China(82060695)Guangxi Zhuang Autonomous Region Philosophy and Social Science Planning Research Project(20BMZ005)Foreign Expert Project of the Ministry of Science and Technology of the People s Republic of China(GXL20200233001)Guangxi First-class Discipline of Traditional Chinese Medicine:Ethnic Medicine(GJKY[2018]12).
文摘[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.
基金Supported by Zhuang Yao Medicines Collaborative Innovation Center(Gui JKY[2013]No.20)Guangxi Zhuang Yao Medicines Key Laboratory(Gui KJZ[2014]No.32)+1 种基金Guangxi Key Disciplines on Zhuang Medicine(Gui JKY[2013]No.16)Studies of Bagui Scholars on Innovation Theory and Efficacy of TCM
文摘Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and preventing phlegm form forming and stopping coughing. In this paper,the research on the herbal medicine,medicinal identification,chemical composition and extraction process of some chemical components,trace elements,clinical application and pharmacological action,antibacterial effect were reviewed. The existing problems in the current research were analyzed,and its future research direction were prospected,with an attempt to provide reference for its future research and development and utilization.
基金Supported by National Science and Technology Support Program(2015BAC05-B02)Sichuan Science and Technology Support Program(2015SZ0034)Education and Teaching Research and Reform Project of Southwest University for Nationalities(2015)
文摘[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identification,character identification,powder identification and paraffin section identification were adopted in the study. [Results]The characteristics of Yi Crude herb of Pholidota chinensis Lindl. were obvious in the original plant,Crude herb and microscopic characteristics. [Conclusions]This paper provided reference for developing quality standards of Yi Crude herb Pholidota chinensis Lindl. and original plant identification,and provided the basis for further research.
文摘从陆英Sam bucus chinensisL ind l.分离并鉴定了5个化合物分别为:β-谷甾醇(Ⅰ)、齐墩果酸(Ⅱ)、熊果酸(Ⅲ)、山柰酚-3-O-β-D-(6-O-乙酰基-葡萄吡喃糖)-7-O-β-D-葡萄吡喃糖苷(Ⅳ)和山柰酚-3-O-β-D-葡萄吡喃糖-7-O-β-D-葡萄吡喃糖苷(Ⅴ),化合物Ⅳ和Ⅴ为首次从陆英中分离得到。
