The main objective of this study was to evaluate the biopsy sampling procedure in the detection of esophageal precancerous lesions. Biopsies were taken one from the middle-third and one from the lower-third of the eso...The main objective of this study was to evaluate the biopsy sampling procedure in the detection of esophageal precancerous lesions. Biopsies were taken one from the middle-third and one from the lower-third of the esopbagus of 28 subjects from a high incidence area for esophageal cancer in Henan, China. The sampling procedure was repeated on the same subjects 10 days later.During this period, all subjects followed their usual dietary habits. Histopathological analysis showed that in the second sampling from the middle-third of the esophagus, 24% of the subjects had less severe lesions,28% had more severe lesions and 48% had the same severity oflesions. From the lower-third of the esphagus,48% of the subjects had less severe lesions, 16% had more severe lesions and 32% had the same severity of lesions.These results suggest that the reproducibility of biopsy procedure could be a factor for the previously reported 'reversion' or 'progression' of precancerous lesions in follow-up studies.展开更多
Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding ...Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding the fate and risk of this class of emerging pollutants. However, the wide use of OPEs in experimental materials results in blank interference, which influences the accuracy of analytical results. In the present work, blank contamination and recovery of pretreatment procedures for analysis of OPEs in water samples were systematically examined for the first time. Blank contaminations were observed in filtration membranes, glass bottles, solid phase extraction cartridges, and nitrogen blowing instruments. These contaminations could be as high as 6.4–64 ng/L per treatment. Different kinds of membranes were compared in terms of contamination levels left after common glassware cleaning, and a special wash procedure was proposed to eliminate the contamination from membranes. Meanwhile, adsorption of highly hydrophobic OPEs on the inside wall of glass bottles was found to be 42.4%–86.1%, which was the primary cause of low recoveries and was significantly reduced by an additional washing step with acetonitrile. This work is expected to provide guidelines for the establishment of analysis methods for OPEs in aqueous samples.展开更多
文摘The main objective of this study was to evaluate the biopsy sampling procedure in the detection of esophageal precancerous lesions. Biopsies were taken one from the middle-third and one from the lower-third of the esopbagus of 28 subjects from a high incidence area for esophageal cancer in Henan, China. The sampling procedure was repeated on the same subjects 10 days later.During this period, all subjects followed their usual dietary habits. Histopathological analysis showed that in the second sampling from the middle-third of the esophagus, 24% of the subjects had less severe lesions,28% had more severe lesions and 48% had the same severity oflesions. From the lower-third of the esphagus,48% of the subjects had less severe lesions, 16% had more severe lesions and 32% had the same severity of lesions.These results suggest that the reproducibility of biopsy procedure could be a factor for the previously reported 'reversion' or 'progression' of precancerous lesions in follow-up studies.
基金financially supported by the National High Technology Research and Development Program of China (No.2013AA065201)Chinese Academy of Sciences (Nos.XDB14020101, YSW2013A01, YSW2013B01)
文摘Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding the fate and risk of this class of emerging pollutants. However, the wide use of OPEs in experimental materials results in blank interference, which influences the accuracy of analytical results. In the present work, blank contamination and recovery of pretreatment procedures for analysis of OPEs in water samples were systematically examined for the first time. Blank contaminations were observed in filtration membranes, glass bottles, solid phase extraction cartridges, and nitrogen blowing instruments. These contaminations could be as high as 6.4–64 ng/L per treatment. Different kinds of membranes were compared in terms of contamination levels left after common glassware cleaning, and a special wash procedure was proposed to eliminate the contamination from membranes. Meanwhile, adsorption of highly hydrophobic OPEs on the inside wall of glass bottles was found to be 42.4%–86.1%, which was the primary cause of low recoveries and was significantly reduced by an additional washing step with acetonitrile. This work is expected to provide guidelines for the establishment of analysis methods for OPEs in aqueous samples.
