Spectral quantitative fingerprinting including ultraviolet (UV) and Fourier transform infrared (FT-IR) coupled with combustion heat (CH) analytical techniques was employed and compared for rapid screening quality grad...Spectral quantitative fingerprinting including ultraviolet (UV) and Fourier transform infrared (FT-IR) coupled with combustion heat (CH) analytical techniques was employed and compared for rapid screening quality grade and discriminating San-Huang Tablets (SHT) of different commercial brands. The systematic quantified fingerprint method (SQFM) was applied to evaluate, qualitatively and quantitatively, the quality consistency of the herbal preparation. It was possible to deduce that the quantitative similarity analysis by SQFM was enabled to make a good discrimination of the tested samples. It was a particularly useful method for the overall quality evaluation of herbal medicine and their preparations.展开更多
The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets(SHT), a widely used and commercially available herbal preparation, where high performance liquid ...The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets(SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography(HPLC) with a diode array detector(DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method(SLQFM)coupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively. Additionally, the component–activity relationship between chromatographic fingerprints and total radical-scavenging capacity in vitro(as assessed using the 1, 1-diphenyl-2-picrylhydrazyl(DPPH) assay) was investigated by partial least squares regression(PLSR) analysis to predict the antioxidant capacity of new samples from the chromatographic fingerprints and identify the main active constituents that can be used as the target markers for the quality control of SHT. In conclusion, the strategy developed in the present study was effective and reliable, which can be employed for holistic evaluation and accurate discrimination for the quality consistency of SHT preparations and other traditional Chinese medicine(TCM) and herbal preparations as well.展开更多
目的研究补肾益脑片对快速衰老小鼠(SAM鼠)生理及其脑组织基因表达的影响。方法采用DDRT-PCR方法对给药与否的SAM小鼠脑组织mRNA表达变化进行分析。结果补肾益脑片能提高血虚小鼠血红蛋白水平和红细胞数,改善小鼠的空间学习记忆功能和...目的研究补肾益脑片对快速衰老小鼠(SAM鼠)生理及其脑组织基因表达的影响。方法采用DDRT-PCR方法对给药与否的SAM小鼠脑组织mRNA表达变化进行分析。结果补肾益脑片能提高血虚小鼠血红蛋白水平和红细胞数,改善小鼠的空间学习记忆功能和对条件性刺激的逃避反应。本研究鉴定了14个差异表达条带,并对其中的3个条带进行了测序分析,序列比较显示,其序列分别与脂肪酸结合蛋白-7(fatty acid binding protein 7)、还原型辅酶Q-细胞色素c还原酶复合体7．2kD亚单位[ubiquinol-cyto- chrome C reductase complex(7．2 kD)]和核糖体大亚基的第21亚单位(60S ribosomal protein L21)的mRNA同源。其同源性分别为97%、100%、99%。结论补肾益脑片可影响小鼠生理变化；在分子水平上是通过调节小鼠脑组织基因表达水平来发挥其抗衰老功效的。展开更多
文摘Spectral quantitative fingerprinting including ultraviolet (UV) and Fourier transform infrared (FT-IR) coupled with combustion heat (CH) analytical techniques was employed and compared for rapid screening quality grade and discriminating San-Huang Tablets (SHT) of different commercial brands. The systematic quantified fingerprint method (SQFM) was applied to evaluate, qualitatively and quantitatively, the quality consistency of the herbal preparation. It was possible to deduce that the quantitative similarity analysis by SQFM was enabled to make a good discrimination of the tested samples. It was a particularly useful method for the overall quality evaluation of herbal medicine and their preparations.
基金financially supported by the National Natural Science Foundation of China(No.81560695)the Doctoral Research Fund of Dali University(No.KYBS201512)
文摘The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets(SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography(HPLC) with a diode array detector(DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method(SLQFM)coupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively. Additionally, the component–activity relationship between chromatographic fingerprints and total radical-scavenging capacity in vitro(as assessed using the 1, 1-diphenyl-2-picrylhydrazyl(DPPH) assay) was investigated by partial least squares regression(PLSR) analysis to predict the antioxidant capacity of new samples from the chromatographic fingerprints and identify the main active constituents that can be used as the target markers for the quality control of SHT. In conclusion, the strategy developed in the present study was effective and reliable, which can be employed for holistic evaluation and accurate discrimination for the quality consistency of SHT preparations and other traditional Chinese medicine(TCM) and herbal preparations as well.
文摘目的研究补肾益脑片对快速衰老小鼠(SAM鼠)生理及其脑组织基因表达的影响。方法采用DDRT-PCR方法对给药与否的SAM小鼠脑组织mRNA表达变化进行分析。结果补肾益脑片能提高血虚小鼠血红蛋白水平和红细胞数,改善小鼠的空间学习记忆功能和对条件性刺激的逃避反应。本研究鉴定了14个差异表达条带,并对其中的3个条带进行了测序分析,序列比较显示,其序列分别与脂肪酸结合蛋白-7(fatty acid binding protein 7)、还原型辅酶Q-细胞色素c还原酶复合体7．2kD亚单位[ubiquinol-cyto- chrome C reductase complex(7．2 kD)]和核糖体大亚基的第21亚单位(60S ribosomal protein L21)的mRNA同源。其同源性分别为97%、100%、99%。结论补肾益脑片可影响小鼠生理变化；在分子水平上是通过调节小鼠脑组织基因表达水平来发挥其抗衰老功效的。
