Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were...Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were obtained from the Traditional Chinese Medicine Systems and Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine(BATMAN-TCM)database,and the literature.The component-target regulatory network and protein-protein interaction(PPI)network were constructed by Cytoscape 3.7.2,and a bioinformatics analysis was performed to identify the significant pathways and their relevant targets.Molecular docking of the core active components and relevant targets was performed.Results A total of 98 active components of SAD and the corresponding 113 drug targets were identified.The component-target regulatory network and PPI network were successfully established.Results of the bioinformatics analysis indicated that 2281 Gene Ontology(GO)terms were enriched in chronic cough,including 2062 terms were in biological processes,77 in cellular components,and 142 in molecular functions,and top 20 significant pathways in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Molecular docking study demonstrated that quercetin,luteolin,kaempferol,and naringenin were in good agreement with the corresponding targets.Conclusion The active compounds of SAD,such as quercetin,luteolin,kaempferol,and naringenin,may act on AKT1,MAPK1,RELA,EGFR,and Bcl-2 and regulate the PI3 K-Akt signaling pathway,AGE-RAGE signaling pathway,and fluid shear stress and atherosclerosis pathway to exert the effects of anti-inflammatory,anti-airway remodeling,anti-oxidant stress effects,and repair airway damage,thus treating chronic cough.展开更多
目的:观察三拗汤对哮喘模型大鼠转化生长因子β-1及神经生长因子表达的影响,并探究其可能的机制。方法:40只SD大鼠均分为正常对照组、哮喘模型组、布地奈德组(2.5×10-4g/kg)及三拗汤组(39 g/kg)。以鸡卵清蛋白致敏加激发法复制哮...目的:观察三拗汤对哮喘模型大鼠转化生长因子β-1及神经生长因子表达的影响,并探究其可能的机制。方法:40只SD大鼠均分为正常对照组、哮喘模型组、布地奈德组(2.5×10-4g/kg)及三拗汤组(39 g/kg)。以鸡卵清蛋白致敏加激发法复制哮喘模型,最终测量气道壁厚度及气道平滑肌厚度;酶联免疫吸附法检测支气管肺泡灌洗液及血清中转化生长因子β-1的浓度;免疫组化法检测神经生长因子表达水平。结果:与正常对照组相比,哮喘模型组气道壁厚度及气道平滑肌厚度均增加(15.08±0.41、10.52±0.28 vs 43.65±1.25、32.64±1.18,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度上升(58.75±1.96、98.13±4.44 vs 115.88±3.47、195.89±6.18,P<0.01),肺组织中神经生长因子表达水平升高(97.15±3.05 vs 165.87±5.12,P<0.01);与哮喘模型组比较,布地奈德组(2.5×10-4g/kg)、三拗汤组(39 g/kg)中气道壁厚度及气道平滑肌厚度均减小(43.65±1.25、32.64±1.18 vs 22.15±0.62、16.43±0.45;21.47±0.95、16.10±0.49,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度下降(115.88±3.47、195.89±6.18 vs 84.05±2.86、138.80±4.90;79.62±3.26、141.03±5.87,P<0.01),肺组织中神经生长因子表达水平降低(165.87±5.12 vs 117.55±3.72;114.35±3.43,P<0.01);布地奈德组、三拗汤组上述指标间比较,差异无统计学意义。结论:三拗汤对减轻哮喘大鼠气道重塑具有明显的影响,其机制可能与降低TGFβ-1浓度及下调肺组织中NGF蛋白表达水平相关。展开更多
基金funding support from the National Natural Science Foundation of China(No.82174093)。
文摘Objective To investigate the active components and mechanism of Sanao Decoction(三拗汤,SAD)in treating chronic cough based on network pharmacology and molecular docking.Methods Active components and their targets were obtained from the Traditional Chinese Medicine Systems and Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine(BATMAN-TCM)database,and the literature.The component-target regulatory network and protein-protein interaction(PPI)network were constructed by Cytoscape 3.7.2,and a bioinformatics analysis was performed to identify the significant pathways and their relevant targets.Molecular docking of the core active components and relevant targets was performed.Results A total of 98 active components of SAD and the corresponding 113 drug targets were identified.The component-target regulatory network and PPI network were successfully established.Results of the bioinformatics analysis indicated that 2281 Gene Ontology(GO)terms were enriched in chronic cough,including 2062 terms were in biological processes,77 in cellular components,and 142 in molecular functions,and top 20 significant pathways in Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Molecular docking study demonstrated that quercetin,luteolin,kaempferol,and naringenin were in good agreement with the corresponding targets.Conclusion The active compounds of SAD,such as quercetin,luteolin,kaempferol,and naringenin,may act on AKT1,MAPK1,RELA,EGFR,and Bcl-2 and regulate the PI3 K-Akt signaling pathway,AGE-RAGE signaling pathway,and fluid shear stress and atherosclerosis pathway to exert the effects of anti-inflammatory,anti-airway remodeling,anti-oxidant stress effects,and repair airway damage,thus treating chronic cough.
文摘目的:观察三拗汤对哮喘模型大鼠转化生长因子β-1及神经生长因子表达的影响,并探究其可能的机制。方法:40只SD大鼠均分为正常对照组、哮喘模型组、布地奈德组(2.5×10-4g/kg)及三拗汤组(39 g/kg)。以鸡卵清蛋白致敏加激发法复制哮喘模型,最终测量气道壁厚度及气道平滑肌厚度;酶联免疫吸附法检测支气管肺泡灌洗液及血清中转化生长因子β-1的浓度;免疫组化法检测神经生长因子表达水平。结果:与正常对照组相比,哮喘模型组气道壁厚度及气道平滑肌厚度均增加(15.08±0.41、10.52±0.28 vs 43.65±1.25、32.64±1.18,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度上升(58.75±1.96、98.13±4.44 vs 115.88±3.47、195.89±6.18,P<0.01),肺组织中神经生长因子表达水平升高(97.15±3.05 vs 165.87±5.12,P<0.01);与哮喘模型组比较,布地奈德组(2.5×10-4g/kg)、三拗汤组(39 g/kg)中气道壁厚度及气道平滑肌厚度均减小(43.65±1.25、32.64±1.18 vs 22.15±0.62、16.43±0.45;21.47±0.95、16.10±0.49,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度下降(115.88±3.47、195.89±6.18 vs 84.05±2.86、138.80±4.90;79.62±3.26、141.03±5.87,P<0.01),肺组织中神经生长因子表达水平降低(165.87±5.12 vs 117.55±3.72;114.35±3.43,P<0.01);布地奈德组、三拗汤组上述指标间比较,差异无统计学意义。结论:三拗汤对减轻哮喘大鼠气道重塑具有明显的影响,其机制可能与降低TGFβ-1浓度及下调肺组织中NGF蛋白表达水平相关。
