[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,ar...[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,aroma and taste as an indicator,the preparation process of tea bags was optimized using an orthogonal test.[Results]The results of the orthogonal test show that the optimal preparation process for the Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags are as follows:particle size of 14 meshes,solid to liquid ratio of 1∶40,brewing temperature of 70℃,and brewing time of 10 min.[Conclusions]The optimized preparation process is reasonable and feasible.It is suitable for the production of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.展开更多
[Objectives] To establish a HPLC method for the simultaneous determination of three components( namely,quercetin,quercitrin and avicularin) in Herba Taxilli. [Methods]Agilent C18 liquid chromatography column( column s...[Objectives] To establish a HPLC method for the simultaneous determination of three components( namely,quercetin,quercitrin and avicularin) in Herba Taxilli. [Methods]Agilent C18 liquid chromatography column( column size: 150 mm × 4. 6 mm,5 m) was used;mobile phase Dacetonitrile∶ water = 22∶ 78; flow rate: 1. 0 m L/min; column temperature: 35℃; injection volume: 10 μL; detection wavelength: 254 nm. [Results]The injection volume of quercetin,quercitrin and avicularin was in a good linear relationship with the peak area in the range of 0. 22-4. 4 μg( R = 1),0. 096-3. 84 μg( R = 0. 999 6),and 0. 106-5. 3 μg( R = 0. 999 9),and the average sample recovery was 99. 32%,100. 53%,and 98. 96%,respectively. [Conclusions]The stability and reproducibility of the established method for determining the content of Herba Taxilli in this experiment are good,and it is expected to provide reference for its quality control and evaluation.展开更多
目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离...目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离体肝脏消耗葡萄糖的影响与肝脏损伤状况。结果受试的中药提取液在120 m in内对灌流液中谷丙转氨酶没有显著性影响,桑白皮、桑枝、桑寄生提取物能显著降低灌流液中葡萄糖的浓度。结论桑白皮、桑寄生、桑枝提取物在胰岛素存在时能增加离体肝脏葡萄糖的消耗。展开更多
基金Supported by Development and Research Center for China-ASEAN Traditional Medicine(05018017)Natural Science Foundation of Guangxi(2014GXNSFBA118195)
文摘[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,aroma and taste as an indicator,the preparation process of tea bags was optimized using an orthogonal test.[Results]The results of the orthogonal test show that the optimal preparation process for the Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags are as follows:particle size of 14 meshes,solid to liquid ratio of 1∶40,brewing temperature of 70℃,and brewing time of 10 min.[Conclusions]The optimized preparation process is reasonable and feasible.It is suitable for the production of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.
基金Supported by Natural Science Foundation Project of Guangxi(2014GXNSFBA118195)Talent Training Construction Program of Team of Professor Yang Shilin in Guangxi University of Chinese Medicine(YSL17005)Project of National Natural Science Foundation(81660669)
文摘[Objectives] To establish a HPLC method for the simultaneous determination of three components( namely,quercetin,quercitrin and avicularin) in Herba Taxilli. [Methods]Agilent C18 liquid chromatography column( column size: 150 mm × 4. 6 mm,5 m) was used;mobile phase Dacetonitrile∶ water = 22∶ 78; flow rate: 1. 0 m L/min; column temperature: 35℃; injection volume: 10 μL; detection wavelength: 254 nm. [Results]The injection volume of quercetin,quercitrin and avicularin was in a good linear relationship with the peak area in the range of 0. 22-4. 4 μg( R = 1),0. 096-3. 84 μg( R = 0. 999 6),and 0. 106-5. 3 μg( R = 0. 999 9),and the average sample recovery was 99. 32%,100. 53%,and 98. 96%,respectively. [Conclusions]The stability and reproducibility of the established method for determining the content of Herba Taxilli in this experiment are good,and it is expected to provide reference for its quality control and evaluation.
文摘目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离体肝脏消耗葡萄糖的影响与肝脏损伤状况。结果受试的中药提取液在120 m in内对灌流液中谷丙转氨酶没有显著性影响,桑白皮、桑枝、桑寄生提取物能显著降低灌流液中葡萄糖的浓度。结论桑白皮、桑寄生、桑枝提取物在胰岛素存在时能增加离体肝脏葡萄糖的消耗。
