Background： Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count（SCC） and somatic cell score（SCS）, serum cytokines such as interleukin-17（IL-17...Background： Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count（SCC） and somatic cell score（SCS）, serum cytokines such as interleukin-17（IL-17） and interleukin-4（IL-4） may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms（SNPs） in bovine IL-17 F and IL-17 A genes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the m RNA expression variations of the cows with different genotypes.Results： A total of 464 lactating cows（337 Holstein and 127 Inner-Mongolia Sanhe cattle） were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436 C ＆gt; T in IL-17 F and SNP g.24345410 A ＆gt; G in IL-17 A showed significant effects on SCC and IL-4 in Holstein（n = 337） and on IL-17 and IL-4 in Sanhe cattle（n = 127）. The homozygous GG genotype of SNP g.24345410 A ＆gt; G had significantly higher m RNA expression compared with the heterozygous AG genotype.Conclusions： The results indicate that IL-17 F and IL-17 A could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.展开更多
[ Objective] The aim was to analyze the ground and shallow ground temperature trend in Sanhe in recent 40 years. [ Method] According to the annual and seasonal average ground temperature in 2010, by dint of line chart...[ Objective] The aim was to analyze the ground and shallow ground temperature trend in Sanhe in recent 40 years. [ Method] According to the annual and seasonal average ground temperature in 2010, by dint of line charts and trend diagram, the ground temperature in Sanhe City in recent 40 years was discussed. [ Result] The annual ground temperature, annual ground highest and lowest temperature in Sanhe City in recent 40 years was increasing. The lowest temperature in annual and seasonal surface temperature increased at fastest speed. The annual changes of the 5, 10, 15 and 20 cm ground temperature was decreasing and the 20 cm ground temperature decreased most significantly. In spring, the 5 -15 cm ground temperature increased and the 20 cm reduced. In summer, the 20 cm temperature decreased significantly. In autumn, the 10 cm ground temperature enhanced fastest. In winter, the 10 cm and 15 cm temperature increase rate was large and the 15 cm temperature increased fastest. [ Conclusion] The study provided reference for the influence of global warming on the ground and shallow ground temperature.展开更多
基金financially supported by the National Natural Science Foundation of China(31272420)the Earmarked Fund for Modern Agro-industry Technology Research System(CARS-37)+2 种基金the Fund for Basic Research from the Ministry of Education of the People’s Republic of China(2011JS006)the National Key Technologies R&D Program(2011BAD28B02)the Program for Changjiang Scholar and Innovation Research Team in University(IRT1191)
文摘Background： Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count（SCC） and somatic cell score（SCS）, serum cytokines such as interleukin-17（IL-17） and interleukin-4（IL-4） may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms（SNPs） in bovine IL-17 F and IL-17 A genes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the m RNA expression variations of the cows with different genotypes.Results： A total of 464 lactating cows（337 Holstein and 127 Inner-Mongolia Sanhe cattle） were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436 C ＆gt; T in IL-17 F and SNP g.24345410 A ＆gt; G in IL-17 A showed significant effects on SCC and IL-4 in Holstein（n = 337） and on IL-17 and IL-4 in Sanhe cattle（n = 127）. The homozygous GG genotype of SNP g.24345410 A ＆gt; G had significantly higher m RNA expression compared with the heterozygous AG genotype.Conclusions： The results indicate that IL-17 F and IL-17 A could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.
文摘[ Objective] The aim was to analyze the ground and shallow ground temperature trend in Sanhe in recent 40 years. [ Method] According to the annual and seasonal average ground temperature in 2010, by dint of line charts and trend diagram, the ground temperature in Sanhe City in recent 40 years was discussed. [ Result] The annual ground temperature, annual ground highest and lowest temperature in Sanhe City in recent 40 years was increasing. The lowest temperature in annual and seasonal surface temperature increased at fastest speed. The annual changes of the 5, 10, 15 and 20 cm ground temperature was decreasing and the 20 cm ground temperature decreased most significantly. In spring, the 5 -15 cm ground temperature increased and the 20 cm reduced. In summer, the 20 cm temperature decreased significantly. In autumn, the 10 cm ground temperature enhanced fastest. In winter, the 10 cm and 15 cm temperature increase rate was large and the 15 cm temperature increased fastest. [ Conclusion] The study provided reference for the influence of global warming on the ground and shallow ground temperature.
