Comparison on Immune Efficacy of Avian Influenza Virus Re-5 Strain and SS Strain in Guangxi Sanhuang Chickens

[ Objective] To compare immune efficacy of avian influenza virus （AIV） Re-5 strain （HSN1 subtype） and SS strain （ H9 subtype） in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a breeding chicken farm in Guilin City of Guangxi and assigned into four groups （ n = 45）. At 145 days old, the chickens in the group I and II were respectively inoculated with AIV Re-5 strain and AIV SS strain, but they were kept in cages separately. The two control groups were not inoculated. Antibody levels were determined at 170 days old. [ Result] The level of antibodies against AIV Re-5 strain was significantly higher in the chickens of the group I and group II than in the corresponding control groups, respectively （ P 〈 0.01 ）. The level of antibodies against AIV SS strain was not significantly different between the group I and its control group or between the group II and its control group. [ Conclusion] The antibody level of chickens can be increased by the AIV Re-5 strain but not by the AIV SS strain.

Effects of Diet with "Lianteng" Additive of Chinese Herbal Medicine on Blood Biochemical Indexes,Immune and Antioxidant Function of Guangxi Sanhuang Chickens

[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of"Lianteng"additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of"Lianteng"additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens.