Background:Insomnia is a sleep disorder,which seriously affects people's physical and mental health.Our experimental study will further explain the efficacy and mechanism of the traditional Chinese medicine prescr...Background:Insomnia is a sleep disorder,which seriously affects people's physical and mental health.Our experimental study will further explain the efficacy and mechanism of the traditional Chinese medicine prescription Sanwei Anmian Decoction as well as the single drug treatment for insomnia,and provide scientific basis for TCM decoction to cure more insomnia patients.Methods:42 mices,half male and half female,were randomly divided into 7 groups,6 in each group,Blank group,Model group,Positive group,Sanwei Anmian Decoction group(abbreviated as Sanwei group),Suanzaoren group,Maidong group,Yuanzhi group.Intraperitoneal injection(ip)of PCPA 350 mg·kg^-1 in other groups except Blank group to establish a mice model of insomnia.On the next day after the successful modeling,10ml·kg^-1 corresponding decoction was given to Sanwei group,Suanzaoren group,Yuanzhi group and Maidong group respectively,0.8mg·kg^-1 estazolam for Positive group,Blank group and the Model group with the same amount of normal saline,three times a day for two days.The body weight and the number of autonomous activities of mice were observed.The sleep rate,sleep latency and sleep time were recorded after the last administration,and the contents of serum 5-hydroxytryptamine(5-HT)and dopamine(DA)were detected.Results:The changes of hair yellowing,alarm,food loss and weight loss in mice showed that the model was successful.After the last administration,compared with the Model group and the Blank group,the weight of mice in Sanwei Anmian Decoction and each disassembled prescription group increased,the autonomous activity decreased,the number of mice fell asleep increased,the concentration of 5-HT in serum increased and the concentration of DA decreased,P<0.01,which was statistically significant.Conclusions:Sanwei Anmian Decoction can effectively improve the symptoms of PCPA insomnia mice model.For single drug,Suanzaoren Decoction has the most obvious effect on the treatment of insomnia.展开更多
Liver injury remains a significant global health problem and has a variety of causes,including oxidative stress(OS),inflammation,and apoptosis of liver cells.There is currently no curative therapy for this disorder.Sa...Liver injury remains a significant global health problem and has a variety of causes,including oxidative stress(OS),inflammation,and apoptosis of liver cells.There is currently no curative therapy for this disorder.Sanwei Ganjiang Prescription(SWGJP),derived from traditional Chinese medicine(TCM),has shown its effectiveness in long-term liver damage therapy,although the underlying molecular mechanisms are still not fully understood.To explore the underlining mechanisms of action for SWGJP in liver injury from a holistic view,in the present study,a systems pharmacology approach was developed,which involved drug target identification and multilevel data integration analysis.Using a comprehensive systems approach,we identified 43 candidate compounds in SWGJP and 408 corresponding potential targets.We further deciphered the mechanisms of SWGJP in treating liver injury,including compound-target network analysis,target-function network analysis,and integrated pathways analysis.We deduced that SWGJP may protect hepatocytes through several functional modules involved in liver injury integrated-pathway,such as Nrf2-dependent anti-oxidative stress module.Notably,systems pharmacology provides an alternative way to investigate the complex action mode of TCM.展开更多
目的:观察藏药三味红花散治疗宫颈糜烂的临床疗效与安全性。方法:使用藏药三味红花散局部上药,治疗15次30 d后进行疗效评估,治疗后60 d跟踪观察32例宫颈糜烂患者,并采用R version 4.0.0统计软件进行疗效研究。结果:本次研究32例患者的...目的:观察藏药三味红花散治疗宫颈糜烂的临床疗效与安全性。方法:使用藏药三味红花散局部上药,治疗15次30 d后进行疗效评估,治疗后60 d跟踪观察32例宫颈糜烂患者,并采用R version 4.0.0统计软件进行疗效研究。结果:本次研究32例患者的症状治疗前后配对样本比较显示,接触性出血等10种症状在治疗前后的变化有统计学差异(P<0.05)。三组不同分度糜烂治疗疗效差异具有统计学意义(P<0.05),轻度患者和中度患者的疗效高于重度患者(P<0.05),轻度患者与中度患者之间的疗效差异无统计学意义(P>0.05),其中轻度糜烂、中度糜烂总治愈率达99%,疗效显著。年龄与糜烂分度之间呈负相关,即随着年龄增大,患重度糜烂的患者数量有减少的趋势(P<0.05)。结论:藏药三味红花散治疗宫颈糜烂临床疗效显著,安全性高,尤其对轻度、中度糜烂具有较高的疗效,值得临床推广。展开更多
基金This work was supported by the National College Students Innovation and Entrepreneurship Training Program(Grant No:201610471013).
文摘Background:Insomnia is a sleep disorder,which seriously affects people's physical and mental health.Our experimental study will further explain the efficacy and mechanism of the traditional Chinese medicine prescription Sanwei Anmian Decoction as well as the single drug treatment for insomnia,and provide scientific basis for TCM decoction to cure more insomnia patients.Methods:42 mices,half male and half female,were randomly divided into 7 groups,6 in each group,Blank group,Model group,Positive group,Sanwei Anmian Decoction group(abbreviated as Sanwei group),Suanzaoren group,Maidong group,Yuanzhi group.Intraperitoneal injection(ip)of PCPA 350 mg·kg^-1 in other groups except Blank group to establish a mice model of insomnia.On the next day after the successful modeling,10ml·kg^-1 corresponding decoction was given to Sanwei group,Suanzaoren group,Yuanzhi group and Maidong group respectively,0.8mg·kg^-1 estazolam for Positive group,Blank group and the Model group with the same amount of normal saline,three times a day for two days.The body weight and the number of autonomous activities of mice were observed.The sleep rate,sleep latency and sleep time were recorded after the last administration,and the contents of serum 5-hydroxytryptamine(5-HT)and dopamine(DA)were detected.Results:The changes of hair yellowing,alarm,food loss and weight loss in mice showed that the model was successful.After the last administration,compared with the Model group and the Blank group,the weight of mice in Sanwei Anmian Decoction and each disassembled prescription group increased,the autonomous activity decreased,the number of mice fell asleep increased,the concentration of 5-HT in serum increased and the concentration of DA decreased,P<0.01,which was statistically significant.Conclusions:Sanwei Anmian Decoction can effectively improve the symptoms of PCPA insomnia mice model.For single drug,Suanzaoren Decoction has the most obvious effect on the treatment of insomnia.
基金supported by the National Natural Science Foundation of China(Nos.81603318,81473740,and 81673627)Guangdong Provincial Major Science and Technology for Special Program of China(No.2015A030302072)+2 种基金Guangdong Provincial Major Science and Technology for Special Program of China(No.2015A030302071)the Guangzhou Science Technology and Innovation Commission Technology Research Projects(No.201805010005)the Guangzhou Science Technology and Innovation Commission Technology Research Projects(No.201607010333)
文摘Liver injury remains a significant global health problem and has a variety of causes,including oxidative stress(OS),inflammation,and apoptosis of liver cells.There is currently no curative therapy for this disorder.Sanwei Ganjiang Prescription(SWGJP),derived from traditional Chinese medicine(TCM),has shown its effectiveness in long-term liver damage therapy,although the underlying molecular mechanisms are still not fully understood.To explore the underlining mechanisms of action for SWGJP in liver injury from a holistic view,in the present study,a systems pharmacology approach was developed,which involved drug target identification and multilevel data integration analysis.Using a comprehensive systems approach,we identified 43 candidate compounds in SWGJP and 408 corresponding potential targets.We further deciphered the mechanisms of SWGJP in treating liver injury,including compound-target network analysis,target-function network analysis,and integrated pathways analysis.We deduced that SWGJP may protect hepatocytes through several functional modules involved in liver injury integrated-pathway,such as Nrf2-dependent anti-oxidative stress module.Notably,systems pharmacology provides an alternative way to investigate the complex action mode of TCM.
文摘目的:观察藏药三味红花散治疗宫颈糜烂的临床疗效与安全性。方法:使用藏药三味红花散局部上药,治疗15次30 d后进行疗效评估,治疗后60 d跟踪观察32例宫颈糜烂患者,并采用R version 4.0.0统计软件进行疗效研究。结果:本次研究32例患者的症状治疗前后配对样本比较显示,接触性出血等10种症状在治疗前后的变化有统计学差异(P<0.05)。三组不同分度糜烂治疗疗效差异具有统计学意义(P<0.05),轻度患者和中度患者的疗效高于重度患者(P<0.05),轻度患者与中度患者之间的疗效差异无统计学意义(P>0.05),其中轻度糜烂、中度糜烂总治愈率达99%,疗效显著。年龄与糜烂分度之间呈负相关,即随着年龄增大,患重度糜烂的患者数量有减少的趋势(P<0.05)。结论:藏药三味红花散治疗宫颈糜烂临床疗效显著,安全性高,尤其对轻度、中度糜烂具有较高的疗效,值得临床推广。
