From the stem bark of Sapium sebiferum a new triterpenoid, named sebiferone (1), was isolated. The structure of the new compound was elucidated as 3β-acetoxy-D-friedoolen-14-en -1-one-28-oic acid on the basis of spe...From the stem bark of Sapium sebiferum a new triterpenoid, named sebiferone (1), was isolated. The structure of the new compound was elucidated as 3β-acetoxy-D-friedoolen-14-en -1-one-28-oic acid on the basis of spectral and chemical methods.展开更多
Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the prog...Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the progress of application in biodiesel production.Transcriptome analysis will help to further exploit tallow and kernel oil utilization in industry and facilitate molecular-breeding-based genetic improvement of S.sebiferum.Illumina hiseq 2000 sequencing platform was used to perform de novo transcriptome assembly and gene expression analyses of seeds from three strains in three developmental stages.A total of 188,254 unigenes over 200 bp were assembled with average length of 699 bp.These transcriptic unigenes were functionally assigned by GO and KEGG pathways.RPKM was used to identify differentially expressed unigenes of five cDNA libraries.Transcriptic genes endcoding enzymes related to fatty acid metabolism were evaluated.qRT-PCR was employed to confirm five key enzymes involved in fatty acid and triacylglycerol biosynthesis.This study provided comprehensive gene expression information of Chinese S.sebiferum seed at transcriptional level.These transcriptome data will facilitate high-oil-content genetic cultivation of S.sebiferum,which will further exploit tallow and kernel oil utilization in biodiesel industry.展开更多
This study identifies endogenous inhibitors of germination associated with seed dormancy in Sapium sebiferum,Chinese tallow.Seed extracts were separated into five distinct fractions(petroleum ether,ether,ethyl acetate...This study identifies endogenous inhibitors of germination associated with seed dormancy in Sapium sebiferum,Chinese tallow.Seed extracts were separated into five distinct fractions(petroleum ether,ether,ethyl acetate,methanol,and aqueous)and tested for their effect on germination of cabbage seeds.There were no significant differences in germination percentages and germination index(GI)between the solvents and the control.The five fractions differentially reduced germination percentage and GI values of cabbage seeds.According to the GI results,the greatest inhibition was with the ether fraction followed by methanol,ethyl acetate,petroleum ether,and aqueous fractions.Gas chromatography–mass spectrometry identified the constituents of the four organic fractions.Compared with the mass spectrum of the National Institute of Standards and Technology library,a compound was confirmed,provided it was more than 85%similar.Analysis of ether extracts identified four compounds:butylated hydroxytoluene(BHT;19.7%),1,2-benzenediol(4.1%),phydroxycinnamic acid,ethyl ester(2.8%),and n-hexadecanoic acid(1.4%).Fourteen,20,and 9 compounds were identified in methanol,ethyl acetate,and petroleum ether extracts,respectively.The IC50 of BHT(the concentration at which BHT inhibits radicle growth by 50%)was 54.8 mg/L,indicating that the presence of BHT might be the primary cause of dormancy in S.sebiferum seeds.展开更多
文摘From the stem bark of Sapium sebiferum a new triterpenoid, named sebiferone (1), was isolated. The structure of the new compound was elucidated as 3β-acetoxy-D-friedoolen-14-en -1-one-28-oic acid on the basis of spectral and chemical methods.
基金funded by the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period of China(2011BAD22B08).
文摘Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the progress of application in biodiesel production.Transcriptome analysis will help to further exploit tallow and kernel oil utilization in industry and facilitate molecular-breeding-based genetic improvement of S.sebiferum.Illumina hiseq 2000 sequencing platform was used to perform de novo transcriptome assembly and gene expression analyses of seeds from three strains in three developmental stages.A total of 188,254 unigenes over 200 bp were assembled with average length of 699 bp.These transcriptic unigenes were functionally assigned by GO and KEGG pathways.RPKM was used to identify differentially expressed unigenes of five cDNA libraries.Transcriptic genes endcoding enzymes related to fatty acid metabolism were evaluated.qRT-PCR was employed to confirm five key enzymes involved in fatty acid and triacylglycerol biosynthesis.This study provided comprehensive gene expression information of Chinese S.sebiferum seed at transcriptional level.These transcriptome data will facilitate high-oil-content genetic cultivation of S.sebiferum,which will further exploit tallow and kernel oil utilization in biodiesel industry.
基金provided by the Natural Science Foundation of China(31570662),the Natural Science Foundation of China under Grant 31270711the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutionssupported by the Key Forestry Public Welfare Project under Grant 201304102
文摘This study identifies endogenous inhibitors of germination associated with seed dormancy in Sapium sebiferum,Chinese tallow.Seed extracts were separated into five distinct fractions(petroleum ether,ether,ethyl acetate,methanol,and aqueous)and tested for their effect on germination of cabbage seeds.There were no significant differences in germination percentages and germination index(GI)between the solvents and the control.The five fractions differentially reduced germination percentage and GI values of cabbage seeds.According to the GI results,the greatest inhibition was with the ether fraction followed by methanol,ethyl acetate,petroleum ether,and aqueous fractions.Gas chromatography–mass spectrometry identified the constituents of the four organic fractions.Compared with the mass spectrum of the National Institute of Standards and Technology library,a compound was confirmed,provided it was more than 85%similar.Analysis of ether extracts identified four compounds:butylated hydroxytoluene(BHT;19.7%),1,2-benzenediol(4.1%),phydroxycinnamic acid,ethyl ester(2.8%),and n-hexadecanoic acid(1.4%).Fourteen,20,and 9 compounds were identified in methanol,ethyl acetate,and petroleum ether extracts,respectively.The IC50 of BHT(the concentration at which BHT inhibits radicle growth by 50%)was 54.8 mg/L,indicating that the presence of BHT might be the primary cause of dormancy in S.sebiferum seeds.
