A Novel Phenylpropanoid-substituted Catechin Glycoside and a New Dihydrochalcone from Sarcandra glabra

A novel phenylpropanoid-substituted catechin glycoside glabraoside A 1 and a new dihydrochalcone 3＇-（7＂-allylphenyl）-2＇,4＇,4＂-trihydroxy-6＇-methoxydihydrochalcone 2 were isolated from the herbs of Sarcandra glabra. Their structures were elucidated on the basis of spectroscopic analyses and chiroptical methods.

Study on chromatographic fingerprint of sarcandra glabra (Thunb.) by microwave-assisted extraction coupled to HPLC/DAD

Microwave-assisted extraction(MAE)was used for extraction of effective components of sarcandra glabra(Thunb.),and then chromatographic fingerprint of sarcandra glabra(Thunb.)was studied by high performance liquid chromatography/diode array detector(HPLC/DAD).The conditions of MAE were optimized by an orthogonal experiment,and then the authentication and validation of the chromatographic fingerprint were conducted.Nine peaks were identified as common peaks in the fingerprint chromatograms,and isofraxidin was considered as a reference compound and quantified.Relative standard deviations of retention time and peak area of each component were less than 3% and 8%,respectively.Similarity and difference analysis were conducted by use of PCA and relation coefficient.Twenty batches of sarcandra glabra(Thunb.)samples from two different producing areas could be classified into two different groups in the PCA model.The results showed that MAE-HPLC/DAD method was simple,efficient and stable for the study of complex chromatographic fingerprint of sarcandra glabra(Thunb.),which could provide more reliable and precise information for quality evaluation.

A comprehensive review on the chemical constituents,sesquiterpenoid biosynthesis and biological activities of Sarcandra glabra

Sarcandra glabra(Thunb.)Nakai is a perennial evergreen herb categorised within the Sarcandra Gardner genus under the Chloranthaceae family.Indigenous to tropical and subtropical regions of East Asia and India,this species is extensively distributed across China,particularly in the southern regions(Sichuan,Yunnan,and Jiangxi).In addition to its high ornamental value,S.glabra has a rich history of use in traditional Chinese medicine,evident through its empirical prescriptions for various ailments like pneumonia,dysentery,fractures,bruises,numbness,amenorrhea,rheumatism,and other diseases.Besides,modern pharmacological studies have revealed various biological activities,such as antitumour,anti-bacterial,anti-viral anti-inflammatory and immunomodulatory effects.The diverse chemical constituents of S.glabra have fascinated natural product researchers since the 1900s.To date,over 400 compounds including terpenoids,coumarins,lignans,flavonoids,sterols,anthraquinones,organic acids,and organic esters have been isolated and characterised,some featuring unprecedented structures.This review comprehensively examines the current understanding of S.glabra’s phytochemistry and pharmacology,with emphasis on the chemistry and biosynthesis of its unique chemotaxonomic marker,the lindenane-type sesquiterpenoids.

缙云山草珊瑚(Sarcandra glabra)天然居群结实特征的变异性研究

对缙云山草珊瑚8个天然居群结实特征的20项表型性状的变异性进行了研究,结果表明:各表型性状在居群内与居群间都存在很大的变异性,其中种实产量、果序数和果序分枝的变异程度较高,而果实和种子的特征指数变异程度较低,同类性状果实的变异程度要大于种子;平均变异系数最大的性状是四分枝果序百分比(1.477),最小的是种子长宽比(0.041);变异程度最大的居群是居群6(0.564),最小的是居群4(0.292);各居群间除果序分枝总数差异不显著、果序数和二分枝果序百分比差异显著外,其它17项表型指标均达到极显著水平,表明缙云山草珊瑚各居群间已经发生了明显的形态分化;居群内和居群间变异分别占总变异的60.7%和39.3%,说明居群内变异是表型变异主要来源,但居群间变异也不容忽视;欧氏距离聚类将所有居群划归为两大类群,主要与居群的群落类型与干扰程度有关;表型性状的Pearson相关性聚类显示草珊瑚同类性状在表型分化过程中具有很强的相关性。

Sowing and Seedling Experiment on Different Substrate Nutrient Bags for Sarcandra glabra

A comparative experiment was carried out on sowing and seedling for seeds of Sarcandra glabra( Thunb.) Nakai treated by different methods( A-harvesting and sowing simultaneously with episperm removed; B-harvesting and sowing simultaneously with episperm not removed) and different substrate nutrient bags( A-forest land topsoil; B-yellow soil∶ coarse sand = 1∶ 1; C-farmland soil). Results indicate that the seed germination rate of S. glabra seeds harvested and sowed simultaneously with episperm removed and not removed was as high as 97. 3%and 97. 1%,respectively; the seedling growth of sowing substrate A-forest land topsoil was the highest,the average seedling height,average ground diameter,average main root length,and average lateral root length was 12. 1,0. 21,8. 5 and 7. 0 cm,respectively.

Three new compounds from Sarcandra glabra

One new sesquiterpene lactone,1 α,8 α,9 α-trihydroxyeudesman-3（4）,7（11）-dien-8 β,12-olide（1） and two new phenylpropanoidsubstituted catechin glycosides,glabraoside C（2） and glabraoside D（3） were isolated from the whole plant of Sarcandra glabra. Their structures were established by the analyses of spectral and chemical evidences.

Sesquiterpenoids from the leaves of Sarcandra glabra

Sarglanoids A-F,six new sesquiterpenoids belonging to eudesmane(1-5)and eremophilane(6)types,were isolated from the leaves of Sarcandra glabra,a famous traditional Chinese medicine(TCM).Their structures including absolute configurations were elucidated through extensive spectroscopic analysis and electronic circular dichroism(ECD)calculations.Compounds 1-2 were rare N-containing eudesmane-type sesquiterpenoids.Compound 3 exhibited inhibitory activity against nitric oxide(NO)production in lipopolysaccharides(LPS)-induced RAW 264.7 cells with IC_(50) values at 20.00±1.30μmol·L^(-1).These findings provide scientific evidence for sesquiterpenoids as the material foundation of S.glabra.

Sarcaglarols A-D, Lindenane-Monoterpene Heterodimers from Sarcandra glabra Based on Molecular Networks

Sarcaglarols A-D(1-4),two pairs of lindenane-monoterpene heterodimers fused by a 1,2-dioxane moiety,were discovered and isolated from the leaves of Sarcandra glabra guided by MS/MS molecular networking-based strategy.Their planar structures,absolute configurations of basic skeleton and flexible polyhydric side chain were established by analysis of HRESIMS,NMR spectroscopic data,ECD spectrum,and the X-ray diffraction study of isopropylidene derivatives.An intermolecular[2+2+2]cycloaddition may play a key role in the biosynthesis pathway of the 1,2-dioxane moiety fused lindenane-monoterpene heterodimer skeleton,which can be recognized as the biogenetic precursors of our previous reported lindenane-normonoterpene conjugates.In addition,compounds 1,3 and 4 exhibited moderate inhibitory effects of lipid accumulation in free fatty acid-exposed L02 cells.

Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer Quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra

Objective:The study objective was to determine phenolic components for the quality control(QC)of cultivated Sarcandra glabra(Thunb.)Makino(S.glabra).Materials and Methods:A sensitive,ultra-high-performance liquid chromatography-tandem mass spectrometric method for the simultaneous determination of 12 phenolic components has been developed.Six caffeoylquinic acids,two caffeoylshikimic acids,and four flavanonol glucosides were selected for the comprehensive analysis of distribution in different parts(root,stem,and leaf).Results:Twelve phenolic components were linear in the concentration range of 0.005–5.0μg/mL(R^(2)>0.995).The relative standard deviation of intra-day and inter-day precision across three validation runs over the entire concentration range was<5%.The recovery determined was within 5%in terms of relative error.Our results showed that the 12 phenolic compounds were mainly distributed in leaves and stems far more than those in roots.Conclusions:This study provided an ultra-high-performance liquid chromatograph with triple-quadrupole mass spectrometer quantitative method of 12 phenolic components for the QC of cultivated S.glabra.It was found that the phenolic components were significantly accumulated in the aerial parts(stems and leaves)of cultivated S.glabra.

Clinical observation on prevention and treatment of radiation-induced parotid-gland injury in patients with nasopharyngeal carcinoma by Compound Sarcandra Glabra

Objective:To investigate the protective effect of the Compound Sarcandra Glabra on radiation injury of parotid glands in patients with nasopharyngeal carcinoma.Methods:Sixty patients with primary nasopharyngeal carcinoma in the First Affiliated Hospital of Guangxi Medical University from June 2018 to January 2019 were collected,which were divided into treatment group and control group using a random number table.All patients were treated by a combination of 9-field intense-modulated radiotherapy and chemotherapy.The treatment group was given the Compound Sarcandra Glabra orally every day from 3 days before radiotherapy to the end.The degree of xerostomia(dry mouth)of patients was compared between the two groups at the end of radiotherapy and 3 months after radiotherapy by the chi-square test.The maximal adsorption and secretion rates of the parotid glands were compared between groups before radiotherapy,at the end of radiotherapy and 3 months after radiotherapy by the independent sample t-test.Results:The symptoms of dry mouth were alleviated in the treatment group,and the degree of dry mouth significantly differed between groups at the end of radiotherapy and 3 months after radiotherapy(χ2=6.696 and 10.977,P<0.05).There were no significant differences in the maximal adsorption and secretion rates of the parotid glands between groups before radiotherapy(t=0.974 and4.646,P>0.05);however,both variables were significantly higher in the treatment group at the end of radiotherapy and 3 months after radiotherapy(t=2.920 and 5.037;t=2.820 and 2.213,P<0.05).Patients presented no liver and kidney injury after radiotherapy.Conclusions:Oral administration of the Compound Sarcandra Glabra can effectively prevent radiation injury of the parotid glands in patients with nasopharyngeal carcinoma,thereby alleviating the degree of dry mouth after radiotherapy.

草珊瑚马蓝口腔黏附膜的制备与质量评价

目的:探究草珊瑚马蓝单向释药口腔溃疡黏附膜的制备工艺,并对其质量进行初步评价。方法:选定载药层和隔离层材料并确定其比例,通过改变载药层材料与药物的比例优化膜剂处方,制备草珊瑚马蓝口腔溃疡黏附膜;采用紫外分光光度法测定药物含量;以膜外观性状、重量差异、体外黏附力为检测指标对黏附膜进行质量评价。结果:单向释药黏附膜的制备工艺确定为:载药层CMC-Na与蒸馏水比例为1∶50,且CMC-Na溶液与需加入的药材提取浓缩液比例为1 mL相当于生药0.2 g;过渡层采用1∶1的PVA-1750和CMC-Na;隔离层采用PVA-1750溶液,铺在晾干的CMC-Na载药层和过渡层上,室温晾干脱膜。结论:所制得的单向释药黏附膜为浅黄褐色、表面平整光洁的(2×2)cm^(2)正方型薄膜,无肉眼可见气泡,且具有良好的柔韧性,为进一步研究与应用提供依据。

草珊瑚内生真菌SgG4抗植物病原真菌的活性物质研究

对草珊瑚内生真菌卷曲木霉Trichoderma spirale SgG4抗植物病原真菌的活性物质进行研究。采用菌丝生长速率法对SgG4发酵产物进行抑菌活性测定,采用硅胶柱层析法并结合活性追踪对发酵产物的活性萃取物进行活性物质分离纯化,运用核磁共振谱和质谱技术对活性化合物进行结构鉴定。结果表明,抗菌活性物质主要存在于发酵产物的乙酸乙酯萃取物中,该萃取物对13种植物病原真菌均有较好的抑制活性,EC 50值为0.0002~2.4757 g/L,其中对甘蔗凤梨病菌的抑制活性最好。从发酵产物的乙酸乙酯萃取物中分离出1个抑菌活性化合物,鉴定为木霉酸(trichoderma acid)。木霉酸在0.1 g/L时,对13种植物病原真菌均有抑制作用,其中对茶轮斑病菌、烟草黑胫病菌、甘蔗凤梨病菌和白绢病菌的抑菌率为100%,对其余9种植物病原真菌的抑菌率为21.43%~85.71%。可见,木霉酸的抑菌活性较好且抗菌谱广,在微生物源杀菌剂方面具有较好的开发应用前景。

九节茶药材质量标准研究

目的 对九节茶药材开展全面的质量标准研究,建立完善的九节茶质量标准。方法 收集13批九节茶药材,以异嗪皮啶对照品和九节茶对照药材建立薄层色谱鉴别方法;参考《中国药典》(2020年版)四部通则,对九节茶水分、总灰分、酸不溶性灰分及浸出物进行检查;采用高效液相色谱法测定九节茶中异嗪皮啶和迷迭香酸的含量。结果 九节茶薄层鉴别特征斑点清晰,分离度良好;13批九节茶药材水分平均为9.4%,总灰分平均为5.2%,酸不溶性灰分平均为0.5%,浸出物平均为12.6%;异嗪皮啶平均含量为0.026%,迷迭香酸平均含量为0.030%。结论 本研究建立的薄层鉴别、检查及含量测定方法专属性强、准确度高、操作简便,可用于九节茶药材质量标准研究。

杉木土壤酚酸物质对草珊瑚生长和品质的影响

【目的】探究杉木人工林土壤中含量较高的4种酚酸物质对草珊瑚生长和品质的化感效应,为杉木林下套种草珊瑚提供理论依据,同时为利用其化感效应指导生产提供参考。【方法】以3个月生草珊瑚为试验材料,分别施加20,40,60,80 mg/L的对羟基苯甲酸、阿魏酸、香草酸和没食子酸处理液,以清水处理为对照,探究其对草珊瑚幼苗生长(苗高增量、地径增量、地上生物量、地下生物量)、光合生理(光合色素含量、净光合速率、蒸腾速率、气孔导度、胞间CO_(2)浓度)和药用成分(异嗪皮啶、绿原酸、迷迭香酸、总黄酮)含量的影响,利用平均敏感指数、相关性分析和主成分分析进行综合评价。【结果】①与对照相比,20~60 mg/L对羟基苯甲酸、阿魏酸和没食子酸总体上对草珊瑚的苗高增量、地径增量、地上生物量、地下生物量以及异嗪皮啶、绿原酸、迷迭香酸和总黄酮单株含量表现出促进作用,而香草酸则仅在质量浓度为20~40 mg/L时表现出促进作用。②与对照相比,4种酚酸在质量浓度为20~40 mg/L时对草珊瑚叶片的净光合速率、气孔导度和蒸腾速率表现为促进作用;在20~60 mg/L时对叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量提升表现为促进作用;在质量浓度为80 mg/L时对胞间CO_(2)浓度表现为促进作用。③综合分析可知,酚酸处理下草珊瑚的光合生理指标与生长指标、药用成分之间有较强的相关性,说明在一定阈值内酚酸物质可通过增强草珊瑚植株的光合作用,进而促进草珊瑚生长及其体内药用成分的累积。【结论】4种杉木土壤酚酸物质对草珊瑚生长和品质整体呈现出低中质量浓度(20~60 mg/L)促进、高质量浓度(80 mg/L)抑制的化感效应,且20 mg/L阿魏酸、60 mg/L对羟基苯甲酸、40 mg/L的香草酸和没食子酸在促进草珊瑚生长和品质提升方面表现较优。

基于UHPLC-Q-TOF/MS技术分析肿节风总黄酮提取物促进巨核细胞分化的效应成分

目的筛选肿节风总黄酮提取物促进巨核细胞分化的效应成分。方法(1)以人巨核细胞白血病细胞(Dami)与人骨髓基质细胞(HS-5)共培养的方式建立巨核细胞分化障碍模型作为评价体系,实验分组:Dami组(Dami)、对照组(Dami+HS-5)、PMA组[Dami+HS-5+5 ng·mL-1佛波醇12-十四酸酯13-乙酸酯(PMA)]、模型组[Dami+HS-5+1%兔抗大鼠血小板血清(APS)+5 ng·mL-1PMA],培养48 h。采用流式细胞术检测巨核细胞分化成熟表面标记分子CD41a、CD61的表达情况。(2)将49只SD雄性大鼠随机分为空白血浆组、15 min组、30 min组、60 min组、90 min组、120 min组、240 min组,每组7只。各给药组大鼠灌胃肿节风总黄酮提取物1.26 g·kg^(-1),在6个设定时间点(15、30、60、90、120、240 min)采血制备肿节风总黄酮提取物经时含药血浆。(3)采用超高效液相色谱-四极杆串联飞行时间质谱法(UHPLC-Q-TOF/MS)对肿节风总黄酮提取物经时含药血浆进行分析,以峰面积构建肿节风总黄酮提取物经时含药血浆中的化学成分随时间变化量矩阵(X矩阵)。将所采集的6个不同时间点的肿节风总黄酮经时含药血浆对巨核细胞分化成熟障碍模型进行干预,采用流式细胞术检测细胞表面分子CD41a、CD61的表达水平,构建肿节风总黄酮提取物经时含药血浆效应矩阵(Y矩阵)。(4)将X矩阵和Y矩阵数据标准化处理后,采用偏最小二乘法(Partial least squares,PLS)计算分析量效关系,以变量重要性投影(Variable importance for projection,VIP)>1为阈值,筛选与细胞表面分子CD41a、CD61变化相关的效应成分,并进行化学成分鉴定,作为肿节风总黄酮提取物中促进巨核细胞分化的潜在效应成分,最后回归评价体系验证其药效。结果(1)与Dami组比较,对照组Dami细胞表面的CD41a表达水平明显升高(P<0.05)。与对照组比较,PMA组Dami细胞表面的CD41a、CD61表达水平均显著升高(P<0.01)。与PMA组比较,模型组Dami细胞表面的CD41a、CD61表达水平均显著降低(P<0.01)。(2)与空白血浆组比较,15、30、60、90、120、240 min各时间点Dami细胞表面分子CD41a、CD61表达水平均显著升高(P<0.01),且CD41a、CD61均在30 min组表达水平最高。在正、负离子模式下筛选出VIP值>1的潜在效应成分,并选取540.3638@12.25与559.2991@11.53两个成分进行药效学验证。559.2991@11.53被鉴定为胡萝卜苷(Daucosterol,Dau),540.3638@12.25被鉴定为迷迭香酸-4-O-β-D-葡萄糖(Rosmarinic acid 4-O-β-Dglucoside,Ros)。Ros、Dau分别干预巨核细胞分化成熟障碍模型后,与模型组比较,Ros及Dau低、中、高剂量组(40、60、80μg·mL-1)的Dami细胞表面的CD41a、CD61表达水平均显著升高(P<0.05,P<0.01)。结论Ros、Dau可能是肿节风总黄酮提取物促进巨核细胞分化的效应成分。

草珊瑚中类胡萝卜素合成的内源激素调控机制分析

【目的】为了探究草珊瑚[Sarcandra glabra(thunb)nakai]不同器官中内源激素对类胡萝卜素差异积累的可能调控机制。【方法】通过代谢组学方法分析草珊瑚中内源激素和类胡萝卜素代谢物在不同器官间的差异分布情况,结合转录组学技术挖掘草珊瑚类胡萝卜素相关差异酶基因,进一步预测参与调控差异酶基因的转录因子及其激素相关顺式响应元件,从而分析内源激素对类胡萝卜素差异积累的可能调控作用。【结果】吲哚-3-羧酸(indole-3-carboxylic acid)、反式茉莉酸[(-)-jasmonic acid]、二氢茉莉酮酸甲酯(ethyl dihydrojasmonate)、油菜素甾酮(castasterone)、油菜素内酯(brassinolide)等7种内源激素在叶中的含量更高,与角黄素(canthaxanthin)、海胆酮(echinenone)、新黄质(neoxanthin)和念珠藻黄素(nostoxanthin)等8种差异代谢物等富集部位一致;而脱落酸(abscisic acid,ABA)、赤霉素4,5-甲氧基水杨酸(gibberellin 4,5-methoxysalicylic acid)、二氢茉莉酸(dihydrojasmonic acid)和5,6-二羟基吲哚(5,6-dihydroxyindole)在根中的含量更高,与脱落酸醛(abscisic aldehyde)、4,4'-二聚戊烯(4,4'-aiapolycopenedial)、花药黄质(antheraxanthin)这3种差异代谢物富集部位一致;关键转录因子MYB106、SPL1、NAC015、ERF064、WRKY44、BHLH116可通过响应AUX、SA、GA、ABA、Me_JA等植物激素的刺激,参与调控类胡萝卜素合成途径的DXS、VDE、ABA2、AOG、ZEP、CYP97C1、DWARF27、CRTISO、LCYB、PSY这10种代谢酶的表达。实时荧光定量PCR(quantitative real-time polymerase chain reaction,RT-qPCR)结果表明,随机选取的8个差异基因表达趋势与转录组测序结果一致。【结论】筛选出草珊瑚叶和根与类胡萝卜素相关的15 种差异内源激素与 11 种差异代谢物,预测了6种转录因子参与调控10 种代谢酶。

草珊瑚地上部分倍半萜苷类成分研究

为探究金粟兰科草珊瑚属植物草珊瑚[Sarcandra glabra (Thunb.) Nakai]中的化学成分,采用硅胶柱色谱、聚酰胺柱色谱、凝胶柱色谱、制备高效液相色谱等色谱方法,从新鲜的草珊瑚地上部分的水萃取部位分离得到10个化合物,包括2个榄香烷倍半萜糖苷类化合物,6个紫罗兰酮类倍半萜糖苷以及2个酚酸类化合物。根据理化性质及核磁共振数据,以上化合物被鉴定为sarcaglabosideC(1)、 sarcaglabosideD(2)、 byzantionosideB(3)、 laurosideE(4)、(4Z)-4-[(3S)-3-(β-Dglucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one(5)、 dihydrovomifoliol-O-β-D-glucopyranoside(6)、(+)-abscisyl-β-D-glucopyranoside (7)、9ξ-O-β-D-glucopyranosyloxy-5-megastigmen-4-one (8)、rosmarinic acid methyl ester (9)、methyl isorinate (10)。其中,化合物3~5,8~9首次从草珊瑚中分离得到。这些化合物的发现进一步丰富了草珊瑚植物中化合物的结构类型,并为后续药理活性研究提供了重要的物质基础。

肿节风提取物上调NKTR抑制小鼠肺癌生长实验研究

目的:探讨肿节风对肺癌的作用及其机制。方法:CCK8法检测肿节风对肺癌细胞LLC细胞活力的影响,LLC小鼠模型评价肿节风体内抗癌活性,蛋白质组学筛选肿节风处理的LLC细胞中的靶蛋白,蛋白质印迹实验验证靶蛋白的表达,流式细胞术检测小鼠外周血T细胞和NK细胞。结果:CCK8检测结果表明,肿节风抑制LLC细胞的增殖。肿节风治疗显著抑制LLC荷瘤小鼠的肿瘤生长。蛋白质组学分析表明,肿节风上调肺癌细胞中NKTR蛋白表达。Western Blot检测结果显示,肿节风干预的肺癌细胞及小鼠肿瘤组织中NKTR蛋白表达量升高。GEPIA数据库分析显示,NKTR在肺癌组织中表达量低于癌旁组织。肿节风治疗的荷瘤小鼠外周血NK细胞和T细胞比例显著升高。结论:肿节风通过上调NKTR表达增强抗肿瘤免疫。

HPLC-MS/MS法同时测定肿节风中8种化学成分含量

目的:采用HPLC-MS/MS同时测定肿节风中的刺五加苷b1、咖啡酸、芦丁、迷迭香酸、绿原酸、隐绿原酸、新绿原酸和异嗪皮啶等8种化学成分含量。方法:采用Waters ACQUITY UPLC?BEH C18色谱柱(2.1 mm×100 mm,1.7μm);以乙腈-水(含0.04%的甲酸)为流动相,梯度洗脱,流速为0.25 mL/min,进样量5μL,柱温35℃;电喷雾离子化源(ESI);多反应监测模式(MRM)进行定性定量分析。结果:在该色谱条件下,肿节风中8种化学成分在各自范围内,峰面积和浓度呈良好的线性关系(r>0.995),平均加样回收率(n=9)为97.85%~102.89%,RSD为0.89~2.36,经过测定6个产地的肿节风中刺五加苷b1、咖啡酸、芦丁、迷迭香酸、绿原酸、隐绿原酸、新绿原酸、异嗪皮啶的含量依次为4.9~40.8μg/g、642.7~1096.5μg/g、5.4~100.6μg/g、908.8~2488.9μg/g、138.8~975.2μg/g、90.9~506.1μg/g、89.6~707.4μg/g、986.1~1840.8μg/g。结论:该方法准确,重现性好,专属性高,可用于肿节风的质量控制。

明溪县肿节风不同部位药效成分含量与土壤理化性质的相关分析

为优化肿节风的栽培技术、探索新的药用部位以替代传统全株入药方式,选取福建省明溪县不同乡镇野生和种植的肿节风,通过对其药效成分含量进行分析,比较各部位药效成分含量差异,并进一步探讨药效成分与土壤理化性质的相关性。结果表明:明溪县不同乡镇肿节风全株药材的异嗪皮啶、迷迭香酸含量远超2020年版《中国药典》所规定的标准限值,其中迷迭香酸含量更是达到药典标准限值的10倍之多。在不同乡镇中,枫溪乡(野生)肿节风的异嗪皮啶含量最高,为0.110%,枫溪乡(种植)肿节风的迷迭香酸含量最高,为0.253%,两者均显著高于其他乡镇的样品。肿节风的异嗪皮啶主要集中于根部,其含量顺序为根>茎>叶;迷迭香酸主要集中于茎部,其含量顺序为茎>根>叶。肿节风药效成分含量与土壤理化性质的相关性分析,发现异嗪皮啶含量与土壤有机质含量呈极显著正相关,与土壤钾含量呈显著正相关;迷迭香酸含量与土壤有机质含量呈极显著正相关。综上,研究结果对于指导肿节风的种植和栽培管理具有重要意义,可以通过调节土壤有机质和钾含量等理化性质,来提高肿节风的药效成分含量,并筛选出明溪县适宜肿节风生长的理想乡镇。