南海软珊瑚Sarcophyton molle化学成分的研究

从中国南海软肉芝软珊瑚(Sarophyton molle)中分离到七个化合物,经紫外光谱(UV),红外光谱(IR),质谱(MS)和核磁共振光谱(~1H NMR,^(13)C NMR)分析,确定了其化学结构。它们是:异新西松烯(1),(E,E,E)-7,8-环氧-1-异丙基-4,8,12-三甲基-十四环-1,3,,11-三烯(2),正十八烷酸(3),鲨肝醇(4),神经酰胺(5),胸腺嘧啶(6),胸腺嘧啶脱氧核苷(7)。其中化合物(2)为新化合物。本文是第一次报道软珊瑚Sarcophytonmolle的化学成分。

软珊瑚Sarcophyton crassocaule中Cembrane型二萜的分离与鉴定

从采自中国南海西沙群岛海域的软珊瑚 Sarcophyton crassocaule中分离得到一种结构新颖的Cembrane型二萜 ,经 1 H NMR,1 3 C NMR,HMQC,1 H-1 HCOSY,NOESY,FABMS和 X射线晶体衍射确定其结构 ,命名为 Sarcocrassolide B它对 P388的细胞毒性为 1 .5 μg/m L.

南海肥厚肉芝软珊瑚Sarcophyton crassocaule化学成分的研究Ⅲ

目的研究采自中国南海海域的肉芝软珊瑚Sarcophyton crassocaule的化学成分。方法采用硅胶柱层析和HPLC等分离手段从南海肉芝软珊瑚的乙酸乙酯提取部位分离得到单体化合物,采用现代波谱技术、物理常数对照以及化学方法对单体化合物的结构进行研究。结果从肉芝软珊瑚Sarcophyton crassocaule中分离得到4个单体化合物。应用化学方法、物理常数对照和现代波谱分析技术对其结构进行解析,确定为：氢醌双苄醚（1）、N-正十六烷酰基-十八碳-4（E）-烯神经鞘氨醇（2）、Δ4,5（E）,Δ8,9（E）-鞘胺醇-正十六碳酰胺（3）、（2S,3S,4R）-N-2-（1,3,4-三羟基-十八烷基）-十八酰胺（4）。结论化合物1为首次得到的天然氢醌双苄醚类化合物;化合物2-4为神经酰胺类化合物。

肉芝软珊瑚Sarcophyton crassocaule中新的多羟基甾醇的分离

In our continuing search of discovering bioactive substances from marine organisms, a sample of soft coral Sarcophyton crassocaule Mosre collected off the Sanya bay, China, was investigated. Two polyhydroxylated sterols 1 and 2 were isolated from the 95% ethanol extract of the sample by chromatography over silica gel. Compound 1, named sarcrasterol, was a new compound, its structure was established as(22R*,24ξ)-methyl-cholest-5-en-3β,22α,25,28-tetraol by spectroscopic methods. Compound 2, a known compound, was first obtained by Kobayashi, however, the relative configuration of C24 has not been determined yet. This paper reports the stereochemistry of compound 2 established as (24S*)-methyl cholest-3β,5β,6α,25-tetraol by means of X-ray single crystal diffraction analysis. Bioassay results show that compound 2 has a moderate activity against human tumor cell lines MGC-803 with an IC50 value of 23.0 μg/mL.

软珊瑚Sarcophyton sp．中一个新西松烷型二萜的结构解析(英文)

对南海软珊瑚Sarcophyton sp.中的西松烷二萜类成份进行研究,从发现具有新颖结构的次生代谢产物。采用多种色谱手段进行分离纯化,通过理化性质和光谱分析确定化合物结构。分离得到1个新的西松烷二萜类化合物,命名为Sarcophyolide A(1),和一个已知西松烷二萜类化合物7α,8β-dihydroxydeepoxysarcophine(2)。利用现代波谱确定新化合物的结构。

软珊瑚Sarcophyton sp.中一个新的西松烷二萜内酯的分离及结构测定

Many cembranes, 14 membered ring diterpenoids, were reported to have antitumor and antimicrobial activities. Soft corals are rich sources of cembrenoid diterpenes. We reported cembrenoids lactones, such as sarcophinone, iso sarcophinone, sarcophine, sartrochine from the species of Sarcophyton in our earlier papers,. In continuation of our studies on the soft coral Sarcophyton , we obtained a new cembranoid lactone, named sarcophydiol(1) from a specimen of Sarcophyton sp . collected around the bay of Sanya, Hainan Island. On the basis of spectroscopic methods, its structure was determined as ( 10S *,11S *) 3,7,11α ,15 tetramethyl 10 β,11β dihydroxy cembr 2 E,6E,14(15)Z trien 16,1 olide(1). The bioactivities of compound 1 were under investigating.

Further New Highly Oxidative Cembranoids from the Hainan Soft Coral Sarcophyton trocheliophorum

Three new highly oxidative cembranoids,sarcophytrols D-F(1-3),were obtained from the South China Sea soft coral Sarcophyton trocheliophorum,along with two known related ones(4 and 5).Their structures were elucidated by extensive spectroscopic analyses and by comparison with literature data.The discovery of these new secondary metabolites enriched the family of cembranoids deduced from the title animal.

肉芝软珊瑚Sarcophytonsp中次生代谢产物的研究

从中国海南岛三亚附近海域采集的肉芝软珊瑚Sarcophytonsp中 ,分离得5个结晶化合物。通过化学和波谱方法鉴定出他们的结构分别是 :24_亚甲基胆甾_1α,3β,5α,6β_四醇 (1) ;24_亚甲基胆甾_4_烯_3β,6β_二醇(2) ;2_十六碳酰胺基_十八_4(E),8(E)_二烯_1,3_二醇 (3) ;(2S,3S,5R)_2_十六碳酰胺基_十八_1,3,5_三醇(4) ;柳珊瑚甾醇(5)。生理活性试验表明 ,化合物1对人体鼻咽癌(CNE2)和胃癌(MGC_803)细胞有强抑制活性 ;化合物2对P_388肿瘤细胞有强抑制活性 ;柳珊瑚甾醇(5)

Growth Rate Assessment of Alcyonacean Sarcophyton glaucum from Northern Hurghada, Red Sea, Egypt

Eighteen specimens of the soft coral Sarcophyton glaucum have been collected and tagged to determine the growth rate using the weight variations technique and to investigate the reproductive season histologically. Six colonies were totally weighed and 12 fragments (produced from three colonies) were marked and weighed. Some samples showed sever shrinkage that lead to death. The maximum semiannual growth rate was recorded in summer (30.05 gm/6 m). The average monthly growth reached 3.95 gm/month. Many factors affect the growth rate such as water currents, turbidity, sedimentation, temperature and reproduction process. The studied species is a dioecious species (unisex), where the Autozooid polyps are the reproductive organs while siphonozooids are sterile. Female gonads were observed to develop at late July before male gonads which began at late September. Larvae releasing started at late January and the gonads are completely spawned in February. Fertilization occurs internally to form planulae larvae which librated completely in February. The oogenesis exhibited prolonged about 23 months, which gave rise to oocytes of different developmental stages in female colonies at any given time, and a spermatogenic cycle of only 10 - 12 months.

乳白肉芝软珊瑚Sarcophyton glaucum的西松烷二萜及生物活性研究

综合运用各种色谱手段,分离纯化了乳白肉芝软珊瑚Sarcophyton glaucum的化学成分。结合波谱数据、文献数据对比和理化性质等,从中共分离鉴定了9个西松烷型二萜类化合物,分别是sefsarcophinolide(1)、(+)-isosarcophine(2)、sarco-militatin D(3)、sarcophytonolide J(4)、(1S,3E,7E,13S)-11,12-epoxycembra-3,7,15-triene-13-ol(5)、sarcophytonin B(6)、(-)-eunicenone(7)、lobophytin B(8)和arbolide C(9),其中化合物1为新化合物。生物活性测试结果表明,化合物2~6显示出微弱乙酰胆碱酯酶抑制活性,化合物5对肿瘤细胞K562具有微弱的细胞毒活性。

Cembrane-type diterpenoids from the South China Sea soft coral Sarcophyton mililatensis

Eight cembrane-type diterpenoids, namely,(t)-(6 R)-6-hydroxyisosarcophytoxide(1),(t)-(6 R)-6-acetoxyisosarcophytoxide(2),(t)-17-hydroxyisosarcophytoxide(3), sarcomililatins A–D(4–7), and sarcomililatol(8), were isolated from the soft coral Sarcophyton mililatensis collected from Weizhou Island, Guangxi Autonomous Region, together with 2 known related analogues,(t)-isosarcophytoxide(9) and(t)-isosarcophine(10). The structures of these compounds were elucidated by a combination of detailed spectroscopic analyses, chemical methods, and comparison with reported data.The absolute configuration of compound 1 was established by the modified Mosher's method, while the absolute configurations of compounds 4 and 5 were assigned by electronic circular dichroism(ECD)spectroscopy and that of compound 8 was established by time-dependent density functional theory electronic circular dichroism(TD-DFT ECD) calculation. In in vitro bioassays, compound 9 displayed significant cytotoxicity against the human cancer cell lines human promyelocytic leukemia cells(HL-60)and human lung adenocarcinoma cells(A-549) with IC_(50) values of 0.7870.21 and 1.2670.80 μmol/L,respectively. Compounds 4 and 9 also showed moderate inhibitory effects on the TNFα-induced Nuclear factor kappa B(NF-κB, a therapeutical target in cancer) activation, showing IC_(50) values of 35.23712.42 and 22.5274.44 μmol/L, respectively.

Polyoxygenated anti-inflammatory biscembranoids from the soft coral Sarcophyton tortuosum and their stereochemistry

Five novel biscembranoids,ximaolides H-L(1-5),along with four known related compounds(6-9) were isolated from the Hainan soft coral Sarcophyton tortuosum.The structures of the new compounds were determined by extensive spectroscopic analysis,quantum chemical calculations,and/or by comparing their CD spectra with those of the known compounds.Compounds 1 and 2 are the first examples of biscembranoids bearing a 1,35-bridged lactone moiety,4 is the first biscembranoid comprising an uncommon oxetane ring,and 5 represents the first 36-peroxyl biscembranoid.Ximaolides Ⅰ(2),K(4) and F(9) exhibited interesting anti-inflammatory activity by the inhibition of LPS-induced TNF-α protein release in RAW264.7 macrophages.

Xishaglaucumins A—J,New Cembranoids with Anti-Inflammatory Activities from the South China Sea Soft Coral Sarcophyton glaucum

Ten new cembrane-type diterpenoids,namely xishaglaucumins A—J(1—10),along with ten known related ones(11—20),have been isolated from the soft coral Sarcophyton glaucum collected off the Xisha Island in the South China Sea.Their structures were elucidated by extensive spectroscopic analysis,quantum mechanical nuclear magnetic resonance(QM-NMR)methods,X-ray diffraction analysis,chemical methods and comparison with the reported data in the literature.The absolute configuration of new compounds 1,5 and known compounds 11,12,16 and 20 were determined either by chemical methods or by X-ray diffraction analysis using Cu Kα(λ=1.5417A).In in vitro bioassay,compound 4 exhibited inhibitory effects on lipopolysaccharide(LPS)-induced inflammatory responses in BV-2 microglial cells.

A Novel Diterpenoid from the Soft Coral Sarcophyton crassocaule

A novel hydroperoxide cembrane-type diterpenoid was isolated from the soft coral Sarcophyton crassocaule collected from the Xisha Islands in the South China Sea. The structure of 3 was established by spectroscopy and X-ray diffraction analysis,named as sarcophyocrassolide A. It exhibited strong cytotoxicity against the P-388 cell line with IC_ 50 value of 0.1 μg/mL.

软珊瑚Sarcophyton glaucum中新型细胞毒性寡肽的纯化与鉴定（英文）

目的:纯化和鉴定软珊瑚Sarcophyton glaucum蛋白水解物中的细胞毒性肽。创新点:首次从软珊瑚S. glaucum中发现具有癌细胞毒性的寡肽,开发了一种有潜力的新型抗癌药物。方法:通过碱性蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和胰蛋白酶产生软珊瑚S. glaucum蛋白质水解物,并通过MTT法评估其对人宫颈癌HeL a细胞的毒性。通过膜超滤、凝胶过滤色谱、固相萃取和反相高效液相色谱等技术进一步提纯肽;采用从头测序法(de novo sequencing)进行肽鉴定;根据所鉴定的序列,进一步确定合成肽的细胞毒性。结论:从软珊瑚S. glaucum的木瓜蛋白酶水解物中鉴定出三种新型肽序列:AGAPGG、AERQ和RDTQ(分子量分别为428.45、502.53和518.53 Da)。此三种寡肽具有对HeLa的细胞毒性,其半最大效应浓度(EC50)值各为8.6、4.9和5.6 mmol/L,为抗癌药物5-氟尿嘧啶的3.3、5.8和5.1倍。当测试对非癌细胞Hek293的毒性时,这三种肽仅显示16%、25%和11%的细胞毒性。因此,AERQ、AGAPGG和RDTQ有很大的潜力成为肽类抗癌药物。

A Novel Sarsolenane Diterpene as a PTPIB Inhibitor from Hainan Soft Coral Sarcophyton trocheliophorum Marenzeller

A novel sarsolenane diterpene, named secodihydrosarsolenone （1）, as a minor component was obtained from the South China Sea soft coral Sarcophyton trocheliophorum Marenzeller. Its structure was elucidated by detailed spectroscopic analysis. Compound 1 exhibited moderate inhibitory activity （IC50= 13.7 μmol·L^-1） against protein tyrosine phosphatase 1B （PTP1B）, a key target for the treatment of type 2 diabetes, representing the first report of PTP1B inhibitory activity for sarsolenane diterpenes. This discovery promotes computational prediction of binding mode between the enzyme and the metabolite, suggesting a crucial role of the residues Tyr46, Ser216 and Arg221 in the binding action.

两种软珊瑚EST-SSR通用性引物的研发

基于短指软珊瑚(Sinularia ceramensis)和花环肉质软珊瑚(Sarcophyton ehrenbergi)转录组数据,比较了上述两种软珊瑚转录组SSR序列的差异。利用合成的100条EST-SSR引物和4属11种30株软珊瑚样本进行通用性筛选。研究表明,在短指软珊瑚和花环肉质软珊瑚转录组数据中分别检测到2914个和2335个微卫星,两种软珊瑚最多的重复类型均为三核苷酸序列重复,优势重复基元均为TA/AT。筛选出8对扩增稳定的EST-SSR引物,Sinularia属和Sarcophyton属的扩增率分别为87.50%和96.43%,Aldersladum属的扩增率为56.25%,Lobophytum属的扩增率为37.50%。这8对种属间通用性较好的EST-SSR标签序列能为今后软珊瑚多样性研究提供借鉴。

Sarcocinerenoids A—J,Eight Rare Capnosane-Type and Two New Cage-Type Cembranoids with Promoting Angiogenesis Activity from the South China Sea Soft Coral Sarcophyton cinereum

Backgroundand Originality Content,Cembranoids are a structurally diverse class of diterpenoids commonly occurring in marine and terrestrial organisms.(+)-Cembrene was the first reported cembranoid diterpenoid discovered in 1962,l and more than 1400 cembranoid diterpenoids have been identified in the last six decades,[2]Cembranoids are characterized by a 14-membered carbocyclic ring,an isopropyl group and three methyl groups,which form their core carbon skeleton.Enzymatic processes such as oxidation,oxidative rearrangements and transannular cyclizations occur in organisms,leading to the formation of distinctive specialized metabolites such as epoxide,lactone,ester,furan,pyrane and other ringbased cembranoids.[2-3]

南海肉芝软珊瑚中的次生代谢产物分析

从采自海南岛三亚湾的一种肉芝软珊瑚Sarcophytonsp 中分离到1个新的对苯二甲酸二酯衍生物 ,通过波谱方法和化学转变测定其结构为对苯二甲酸_1_异丁基_4_(2′_乙基_正己基 )_二酯(1) ;另外 ,还分离鉴定得2个多羟基甾醇 ,24α_甲基_胆甾_5(6)_烯_3β,7α_二醇(2) ,24_亚甲基_胆甾_3β,5α,6β_三醇(3)和α_十六酸_鲨肝醇酯(4) ;化合物2和3对肿瘤细胞有明显抑制作用 ,4具有升白血球作用。

南海软肉芝软珊瑚的化学成分研究

对来自我国南海西沙群岛的软肉芝软珊瑚Ｓａｒｃｏｐｈｙｔｏｎ ｍｏｌｌｅ Ｔｉｘｉｅｒ Ｄｕｒｉｖａｕｌｔ的化学成分进行了研究，从中分离出２个化合物。经光谱数据分析，确定其结构为胸腺嘧啶（Ⅲ）和２４－甲基胆甾－３β，５α，６β，２５－四醇－２５－单乙酸酯（Ⅳ），这些化合物系首次从软珊瑚Ｓａｒｃｏｐｈｙｔｏｎ ｍｏｄｅ中分离得到。