浙江沿海不同地理群体铜藻Sargassum horneri的AFLP分析

铜藻（Sargassum horneri）是浙江沿海重要的褐藻之一,具有较高的生态价值。对铜藻遗传多样性进行分析,可以为其资源保护、人工繁育以及海洋生态修复等提供理论参考。本研究利用AFLP分子标记技术,从64对选择性扩增引物中筛选出20对扩增效果好、带型清晰、条带分布均匀的引物,并用这20对引物对浙江4个铜藻群体进行了分析。结果显示,80个铜藻个体中共检测到有效结合位点407个,其中多态性结合位点270个;铜藻4个群体整体水平上的多态位点百分率（PPL）为66.34%,4个群体各自的多态位点百分率在30.49%～46.54%之间,均值为36.09%。4个铜藻群体的Nei基因多样性指数（H）为0.237 5,Shannon信息指数（I）为0.374 1,4个铜藻群体的总遗传变异（Ht）和群体内遗传变异（Hs）分别为0.314 2和0.256 9,群体间遗传分化系数（Gst）为0.182 3,即群体间遗传变异占总变异的18.23%,即群体内遗传变异占总变异的81.77%,表明群体间遗传分化程度较低。4个铜藻群体间的基因流动系数（Nm）为1.121 4,表明浙江沿海铜藻群体间有一定的基因交流。

不同氮、磷浓度及配比对铜藻(Sargassum horneri)幼苗生长的影响

采用铜藻(Sargassum horneri)幼苗为实验对象,分别以Na NO3和Na H2PO4为氮源和磷源,研究了不同氮磷质量浓度及氮磷配比对铜藻幼苗生长的影响。进行了氮、磷单因子实验、双因子实验以及不同氮磷配比实验,实验时间为8d。结果表明:氮和磷对铜藻幼苗生长影响极显著(P<0.01),且当氮、磷的质量浓度分别为8mg/L、0.4mg/L时,铜藻幼苗的特定生长率最大;交互作用影响不显著(P>0.05),不同质量浓度的氮为主效应;不同氮磷质量浓度比影响极显著(P<0.01),且当氮磷比为10:1时,铜藻幼苗的特定生长率最大。研究结果为铜藻幼苗培育过程中营养盐的合理调控提供了理论依据。

Effects of Temperature and Light on Growth Rate and Photosynthetic Characteristics of Sargassum horneri

The changing environmental factors exerted great influences on coastal macroalgal communities.To study the responses of the brown seaweed Sargassum horneri to temperature and light,S.horneri was cultured under three temperatures(20,25 and 30℃)and three light intensities(30,60,and 120μmol photons m-2 s-1)for seven days.The growth rate,chlorophyll a(Chl a)and carotenoids(Car)contents,chlorophyll fluorescence,and photosynthetic oxygen evolution rate were measured.The results show that the highest relative growth rate(RGR),maximal electron transport rate(rETRmax);the net photosynthetic rate(Pn)were observed at the lowest temperature(20℃)and highest light intensity(120μmol photons m-2 s-1);and the RGR and Pn were significantly inhibited by the highest temperature(30℃),especially at the lowest light intensity(30μmol photons m-2 s-1)(P<0.05).Additionally,the highest light intensity enhanced the non-photochemical quenching(NPQ)even under the highest temperature(30℃),indicating that the higher light intensity could induce photo-protection reaction of thalli.These results suggest that the higher temperature and lower light intensity exerted negative influences on S.horneri.

Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% (ISSR, 99.44%; SRAP, 100.00%), Nei's genetic diversity H (ISSR, 0.107-0.199; SRAP, 0.100-0.153), and Shannon's information index I (ISSR, 0.157-0.291; SRAP, 0.148-0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st ) among the populations correlated well with their geographical distribution (Mantel test R=0.541 5, 0.541 8; P=0.005 0, 0.002 0 and R=0.728 6, 0.641 2; P=0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.

The Brown Algae Saccharina japonica and Sargassum horneri Exhibit Species-Specific Responses to Synergistic Stress of Ocean Acidification and Eutrophication

Ocean acidification and eutrophication are two important environmental stressors.They inevitably impact marine macroalgae,and hence the coastal ecosystem of China.Saccharina japonica,as the main culture species in China,is suffering the harmful golden tide caused by Sargassum horneri.However,it remains unclear whether the detrimental effects of S.horneri on S.japonica cultivation become more severe in future acidified and eutrophic scenario.In this study,we respectively investigated the effects of pCO_(2)(400μatm and 1000μatm)and nutrients(non-enriched and enriched seawater)on the growth,photosynthesis,respiration,chlorophyll contents,and tissue nitrogen of S.japonica and S.horneri.Results indicated that enrichment of nutrients contributed S.horneri to utilize HCO_(3)^(−).The carbon acquisition pathway shifted from HCO_(3)^(−)to CO_(2) in S.japonica,while S.horneri re-mained using HCO_(3)^(−)regulated by nutrient enrichment.S.horneri exhibited better photosynthetic traits than S.japonica,with a higher level of net photosynthetic rate and chlorophyll contents at elevated pCO_(2) and enriched nutrients.Tissue nitrogen also accumulated richly in the thalli of S.horneri under higher pCO_(2) and nutrients.Significant enhancement in growth was only detected in S.horneri under synergistic stress.Together,S.horneri showed competitive dominance in current study.These findings suggest that increasing risk of golden tide in acidified and eutrophic ocean can most likely result in great damage to S.japonica cultivation.

Comparison of the photo-acclimation potential of fl oating and benthic thalli of Sargassum horneri(Phaeophyta)during autumn and winter

Sargassum horneri is a foundational species and an important contributor to the fl oating seaweed stock along the northeastern coast of Asia.In this study,benthic and fl oating thalli of S.horneri were collected from Changdao Island(37°54′N,120°43′E),Bohai Bay,China.We conducted an in-situ and an indoor experiment to study the acclimation potential in S.horneri to abiotic conditions at sea surface in autumn and winter.Both benthic and fl oating thalli were cultured in situ for two months(from October to December)at diff erent depths:0 m above sea level(masl)and 3 m below sea level(mbsl),and their growth rate,biochemical content,and photosynthetic performance were compared.During the fi rst month of culture,the relative growth rate of fl oating thalli was 2-fold greater than that of benthic thalli at 0 masl.The photosynthetic rate of most thalli was signifi cantly higher at 0 masl than at 3 mbsl.In the indoor experiments,fl oating and benthic thalli were exposed to high light intensity(400μmol photons/(m^(2)·s)photosynthetically active radiation(PAR))for 21 d,and their photo-acclimation capacities were compared.Under high light intensity,the two types of thalli showed low maximum quantum yield(F_(v)/F_(m))and light utilisation effi ciency(α)but high light saturation point(E_(k)).Floating thalli showed higher photosynthetic rate and photoprotective ability than benthic thalli at high light intensity.The eff ective quantum yield of photosystem II[Y(II)]of both types of thalli recovered after a 6-day treatment with low light intensity(40μmol photons/(m^(2)·s)).These fi ndings suggest that S.horneri is highly acclimated to the sea surface environment,which possibly contributes to its rapid accumulation and long free-fl oating periods at the sea surface.

Effect of Water Depth on Seasonal Variation in the Chemical Composition of Akamoku, Sargassum horneri (Turner) C. Agardh

The brown alga, Akamoku, also known as Sargassum horneri, was harvested at depths of 1 - 2 and 5 m in the oceanic areas of the Chikuzen Sea off the coast of Fukuoka, Japan, and its chemical composition (moisture, protein, lipid, ash, carbohydrate and total dietary fiber content) was investigated. The aim of this study was to elucidate the relationship between the chemical components and the growth of S. horneri as recyclable resource for a sustainable future. S. horneri at an ocean depth of 5 m grew rapidly. The biomass was abundant from January until April. After maturation and gamete release at the middle of April, plant senescence began occurring, and only short plants remained at the sea bottom. On the other hand, S. horneri at an ocean depth of 1 - 2 m grew slowly and could be harvested until early June. The protein, lipid and total dietary fiber content of S. horneri at an ocean depth of 5 m showed apparent gender-based differences.

Comparison of environmental responding strategies between Ulva prolifera and Sargassum horneri:an in-situ study during the co-occurrence of green tides and golden tides in the Yellow Sea,China in 2017

Large-scale green tides in the Yellow Sea occurred for 13 consecutive years since 2007.The unusual co-occurrence of green tides and golden tides occurred in the Yellow Sea in 2017.The causative species are Ulva prolifera and/or Sargassum horneri.Previous studies on physiological response characteristics of U.prolifera and S.horneri are done in the laboratory mainly,and there is no in-situ comparative study in this regard.In this study,the in-situ physiological response characteristics of both species were measured.The results indicated that cyclic electron flow and antioxidant system play more important roles in protecting U.prolifera,while non-photochemical quenching is more important for adapting to the environment in S.horneri.U.prolifera has a stronger ability to utilize nutrients to rapidly increase its biomass under a suitable condition compared to S.horneri.

响应面优化铜藻(Sargassum horneri)中褐藻多酚的提取及其结构鉴定

目的:对铜藻(Sargassum horneri)中褐藻多酚的提取工艺条件进行优化,并对提取到的褐藻多酚进行初步的结构解析。方法:以褐藻多酚得率为指标,采用超声波辅助提取法,通过单因素试验及响应面分析,对褐藻多酚超声波辅助提取工艺中乙醇体积分数、液固比和超声提取时间三个参数进行了优化。分别采用Folin-Ciocalteu法、2,4-二甲氧基苯甲醛比色法(2,4-dimethoxybenzaldehyde assay,DMBA法)测定铜藻多酚提取物中总酚得率与褐藻多酚得率,计算得率并分析其相关性。对褐藻多酚提取物运用LC-TOF-MS鉴定其褐藻多酚的分子式并推测其结构。结果:响应面试验得到最佳提取条件是:乙醇体积分数为55%,液固比为42∶1 m L/g,超声提取时间为58 min。在优化条件下,铜藻中褐藻多酚得率为(25.16±0.34)mg/100 g。褐藻多酚得率与总酚得率有显著正相关关系(相关系数为0.803)。通过液相色谱仪联用四级杆串联飞行时间质谱仪(LC-TOF-MS)得到褐藻多酚是分子量为374.3036、分子式为C18H14O9的间苯三酚三聚体,推测其结构为Triphlorethol A。结论:通过研究提高了铜藻中褐藻多酚的得率;褐藻多酚得率和总酚得率有显著正相关性;铜藻中含有褐藻多酚Triphlorethol A。

铜藻Sargassum horneri繁殖生物学及种苗培育研究

文章报道了2005～2007年针对铜藻Sargassum horneri有性生殖规律所开展的研究结果,包括生殖托发生、卵的排放、受精卵的发育及其与环境因子的关系。选择了潮间带典型的生态环境定点观察有性繁殖的过程,提出“铜藻以有性生殖为主,残枝营养繁殖为辅,共同维系种群繁衍”的推论。进行了筏式栽培、人工采苗、育苗及附苗基质选择等试验,为未来铜藻工厂化育苗和开展潮下带铜藻种群的恢复提供了科学依据。

铜藻(Sargassum horneri)在营养限制胁迫后对NH_4-N的超补偿吸收研究

以经济马尾藻铜藻(Sargassum horneri)为研究材料,研究了其在营养限制胁迫后对NH_4-N的超补偿吸收情况。实验分营养限制和恢复营养两个阶段进行,每个阶段均设置饥饿处理组、饱和处理组和正常对照组。铜藻在低营养限制胁迫(饥饿)下培养10天后,恢复营养盐培养3天,分别采用次溴酸盐氧化法和锌镉还原法测定培养液中的NH_4-N和NO_3-N的浓度。本文研究发现,饥饿处理组中铜藻吸收氨氮的速率远高于正常对照组和饱和处理组,在恢复培养第一天时,饥饿处理组对NH_4-N的吸收速率最高为14.94μmol/(g·h),与正常对照组及饱和处理组间差异显著。在恢复培养的后两天,三个处理组中铜藻对NH_4-N的吸收速率差异慢慢变小,直至最后几乎相同。三组对NO_3-N没有表现出较高的吸收,最高仅为6.15μmol/(g·h),结果表明:氮源包括NH_4-N和NO_3-N时,铜藻优先选择吸收NH_4-N。实验后称重测定生长速率:对照组、饥饿处理组和饱和组生长率(SGR)分别为8.48%、8.86%、8.01%,ANOVA方差分析表明,三者存在显著差异(P=0.032<0.05),从而证实了铜藻也存在超补偿生长的现象。

中国南麂列岛铜藻Sargassum horneri实地生态学的初步研究

铜藻Sargassum horneri藻株高大，枝叶繁茂，是中国暖温带海域浅海区海藻场的主要连片大型褐藻物种。文章报道了2006年3月-2007年7月开展的南麂列岛铜藻海藻场实地调查结果。结果表明，铜藻繁茂生长在潮流通畅、风浪较为平静海湾的大干潮线以下至-4m浅海岩礁上，漂浮海面形成海藻场；从调查的种群情况来看，野生种群主体以有性生殖长成的1年生藻株为主，残枝再生的2年生藻株只占5％；铜藻的生长和繁殖适温为11～16℃；繁殖盛期水温16～20℃。根据观察，铜藻海藻场近年以来一直在缩小，个别地区已经成片消失。初步提出了“铜藻以有性生殖为主，残枝营养繁殖为辅，共同维持种群繁衍”的推论。恢复与重建铜藻海藻场需要详细了解铜藻的繁殖和种群生态变迁规律，该研究为实现此目的提供了理论帮助。

Growth and Resource Accumulation of Drifting Sargassum horneri(Fucales, Phaeophyta) in Response to Temperature and Nitrogen Supply

The abnormal increase of drifting brown alga Sargassum horneri was initially documented in 2007.It formed blooms along the coast of East China Sea and Yellow Sea in 2017.In this study,we investigated the changes of specific growth rate and resource accumulation of drifting S.horneri in response to temperature and nitrogen richness at different growth stages under laboratory condition.The investigation lasted from June 2015 to April 2016 with the observation made every two months.The results showed that the life cycle consists of a few growth stages dividable with morphological characteristics.The growth can be divided into shedding and withering(August),rapid growing(October to September),slow growing(February),rapid growing(April)and maturation(June)stages.Under the experimental condition,algal segments were found to grow at temperatures ranging from 5 to 25℃in 12 days even when nitrogen is deficient.A significant difference in the special growth rate(SGR)between nitrogen-enriched and nitrogen-removed treatments was found in most months(P<0.05).SGR was lower in August and February than that in other months.Nitrogen and chlorophyll contents in algal segments were different among different temperatures,nitrogen supply and seasons.Nitrogen content was higher in February and April than that in other months in both nitrogen-enriched and nitrogen-removed treatments.The results showed that the demand of S.horneri for nitrogen increased in spring when it grows fast.It is likely that the high temperature and nitrogen concentration in winter and spring lead to the high biomass accumulation of drifting S.horneri.

烯效唑对铜藻(Sargassum horneri)生长及抗氧化系统的影响

本文研究了不同浓度(0,0.1,1.0,4.0和8.0mg/L)的植物生长调节剂烯效唑处理20天内对铜藻(Sargassum horneri)的生长及抗氧化系统的影响。结果表明,烯效唑显著抑制了铜藻的生长,各处理组藻体的长度和比生长速率(SGR)均显著低于对照组,且烯效唑处理浓度越高,抑制作用越明显。0.1和1.0mg/L烯效唑处理组的叶绿素a、类胡萝卜素和可溶性蛋白的含量均显著提高,可溶性糖的含量在前5天内有短暂的促进,而4.0和8.0mg/L处理组的叶绿素a、类胡萝卜素和可溶性蛋白的含量显著下降,而可溶性糖的含量显著升高。抗氧化防御指标的变化也呈现差异性,各处理组的超氧化物歧化酶(SOD)活性在5d时均显著提高,而随着处理时间的延长,低浓度(≤1.0mg/L)处理组SOD活性恢复到对照组水平,高浓度(≥4.0mg/L)处理组SOD活性仍保持在较高水平,显著高于对照组。0.1mg/L处理组的过氧化氢酶(CAT)活性在实验10d时才显著升高,表现为逐渐诱导的现象,丙二醛(MDA)含量在20d时略低于对照组;而其他三个高浓度处理组的CAT活性在实验期间,均显著升高,MDA含量20d时也显著高于对照。以上研究结果丰富了植物生长调节剂对藻类生长影响的研究体系,为烯效唑在大型藻类中的合理应用提供了理论依据。

Transient expression of the enhanced green fluorescent protein(egfp) gene in Sargassum horneri

Sargassum horneri is a macroalga widespread in North Asia-Pacific region, and these years its bloom has caused huge damage to the environment and the economic in China. To make up the blank on genetic engineering research, a transient transformation system for the multicellular marine brown alga S . horneri was established in this research. The algae used in this research were collected from the Yellow Sea of China and verified as a same species S . horneri with analysis of molecular markers. The S . horneri parietal leaves were transformed with the enhanced green fluorescent gene as the reporter by micro-particle bombardment. The results show that green fluorescent protein (GFP) is an eff ective transgene reporter for S . horneri and that particle bombardment is a suitable method for transformation of S . horneri . Through selection of four diff erent promoters for EGFP and six groups’ bombardment characters, the highest transformation efficiency approximately 1.31% was got with the vector pEGFP-N1 at bombardment characters 900 spi and 6 cm distance. This research paves a way for the further research and application of S . horneri .

风浪对枸杞岛铜藻(Sargassum horneri)分布格局的影响

通过潜水调查枸杞岛铜藻的分布特征,并统计西北太平洋近65年台风生成的波浪对枸杞岛产生的影响以及近3年枸杞岛日常风力和风向的变化规律,分析了枸杞岛铜藻分布格局与风浪影响之间的关系。结果表明：铜藻主要分布在枸杞岛西北侧沿岸,且集中分布在平均海平面以下1~4 m较浅的水深范围内,而枸杞岛东到南侧则无铜藻分布,这与85.1%台风生成的波浪影响枸杞岛的东到南侧有关;近年来枸杞岛铜藻数量减少,但近年来台风生成的波浪对枸杞岛产生的影响并没有显著增强,说明铜藻数量的减少并不是由于近年来台风的显著增强而引起;虽然枸杞岛日常风力以西北-北向最强,西北-北向风向的发生频次也较多,但铜藻却集中分布在枸杞岛西北侧沿岸,说明枸杞岛西北侧大面积贻贝养殖筏可能对风浪的影响起到一定缓解作用,保护了近岸岩礁区铜藻的分布与生长。因此,在铜藻垂直分布上移的趋势下,风浪的影响加剧了对铜藻的破坏作用,风浪对枸杞岛铜藻的分布格局起到重要作用。

光强和硝态氮水平对铜藻(Sargassum horneri)生长和光合生理特性的影响

以金潮种铜藻(Sargassum horneri)为对象,探讨了不同硝态氮水平下光照强度对其生长和光合生理特性的影响,以期为金潮暴发机制的研究提供一定的参考。实验设置了高(100μmol·L-1)、低(自然海水,50μmol·L-1) 2个硝态氮浓度和高(150μmol photons·m-2·s-1)、中(60μmol photons·m-2·s-1)、低(10μmol photons·m-2·s-1)三个光照水平,检测了藻体在不同硝态氮浓度和光照条件下适应培养7 d后生长、色素含量、可溶性糖含量、可溶性蛋白含量、氮吸收速率及最大光合放氧速率(Pmax)等生理指标的变化。结果表明:氮浓度相同时,铜藻的生长速率、可溶性糖含量、可溶性蛋白含量、氮吸收速率、Pmax均随光照强度的增加而增加,而色素含量随着光照强度的增加而降低;低氮条件下,除类胡萝卜素外,以上指标在高光处理下均显著高于低光处理(P<0.05);高氮条件下,除可溶性糖外,以上指标在高光条件下也均显著高于低光处理(P<0.05);同一光照强度下,色素及可溶性蛋白含量、氮吸收速率及Pmax随氮浓度的增加而增加;可溶性糖含量随着硝态氮浓度的增加而减少;且在3个光照条件下,高氮处理下藻体的Pmax均显著高于低氮处理。总之,不同光照和氮浓度处理7 d后,高光、高氮适应后的藻体表现出较高的生长和光合作用潜能。铜藻断裂后漂浮于海面极易获得较高光强条件,而富营养海水中的高氮条件会刺激铜藻的光合潜能而使其加速生长,进而加剧铜藻金潮的暴发。

铜藻多酚的体外抗炎和降血糖活性

利用大孔吸附树脂纯化铜藻多酚粗提液,并研究纯化后铜藻多酚的体外抗炎和降血糖活性。利用脂多糖诱导RAW 264.7巨噬细胞建立体外炎症模型,结果发现,铜藻多酚降低炎症介质NO含量的最佳质量浓度为40μg/m L。与模型组相比,30μg/mL的铜藻多酚溶液可使白细胞介素(interleukin,IL)-6、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和IL-1β 3种炎症因子的mRNA水平显著下调,对炎症因子TNF-α的蛋白表达水平有显著抑制作用,但是对炎症因子IL-1β蛋白表达水平抑制作用不显著,铜藻多酚对炎症因子的抑制作用与浓度呈正相关;铜藻多酚对α-葡萄糖苷酶的半数抑制浓度为5.96μg/mL,根据双倒数曲线发现铜藻多酚对α-葡萄糖苷酶的竞争性抑制常数Kic值为0.06μg/mL,非竞争性抑制常数Kiu值为6.68μg/mL,说明铜藻多酚对α-葡萄糖苷酶的抑制类型是非竞争性大于竞争性的混合可逆性抑制。以上结果表明铜藻多酚具有较好的抗炎和降血糖活性,可以作为一种天然的原料,应用于食品、保健品和化妆品等领域。

铜藻多糖制备工艺优化及漂白对其性质的影响

针对铜藻应用传统高温消化工艺提取褐藻多糖时存在的产品得率低、颜色深的问题,本研究尝试在60℃这一较低的Na_(2)CO_(3)消化温度下,通过响应面法对工艺条件进行优化,以褐藻胶、褐藻糖胶的得率及白度指数(whiteness index,WI)的综合评分为评价指标,确定最佳工艺条件。然后,在所得最佳提取条件下改变NaClO添加量,探究其对褐藻胶及褐藻糖胶性质的影响。结果表明,多糖综合评分与消化浓度、时间及NaClO添加量显著相关(P<0.01),最佳提取工艺为:消化时间7.0 h、消化浓度3.7%(w/v)、NaClO(4%,w/v)添加量2.7 mL,得到褐藻糖胶得率9.90%、褐藻胶得率19.95%、褐藻糖胶WI 62.10%、褐藻胶WI 87.83%、综合评分为44.84。漂白工艺对多糖性质影响的研究结果表明,NaClO的添加使褐藻胶分子量、黏度、M/G值下降,且NaClO的适度添加能提高其凝胶强度;同时,添加NaClO使褐藻糖胶的纯度提升,硫酸基含量及抗氧化活性显著提高(P<0.05)。本研究对铜藻褐藻胶及褐藻糖胶两种多糖的制备工艺进行了系统的研究,为铜藻资源的产业化利用提供了理论指导和实践经验。

两种大型海藻提取物对杜氏盐藻生长和代谢活性的促进作用

实验选取绿潮藻提取物和铜藻提取物作为一种高经济价值藻一一杜氏盐藻生长的营养添加剂,与传统的f/2培养基相比,探究两种大型海藻提取物对杜氏盐藻的生长和某些代谢活性(色素、碳水化合物、蛋白质、光合活性)的影响。藻类生理指标和营养成分分析表明,不同浓度的藻华提取物对杜氏盐藻生长的促进效果显著。添加0.26%、0.31%、0.38%的绿潮藻提取物和添加0.24%的铜藻提取物均能够显著促进杜氏盐藻生物量增长、光合色素和营养物质的积累,但对杜氏盐藻的最大光化学效率(F_(v)/F_(m))和有效量子产量(Yield)无显著影响。研究表明,一定浓度的绿潮藻提取物和铜藻提取物能够显著促进杜氏盐藻的生长和营养物质的积累,该发现为藻华防控及利用大型藻华提取物来优化杜氏盐藻的生产提供了新的思路。