This study addresses the problem of global asymptotic stability for uncertain complex cascade systems composed of multiple integrator systems and non-strict feedforward nonlinear systems. To tackle the complexity inhe...This study addresses the problem of global asymptotic stability for uncertain complex cascade systems composed of multiple integrator systems and non-strict feedforward nonlinear systems. To tackle the complexity inherent in such structures, a novel nested saturated control design is proposed that incorporates both constant saturation levels and state-dependent saturation levels. Specifically, a modified differentiable saturation function is proposed to facilitate the saturation reduction analysis of the uncertain complex cascade systems under the presence of mixed saturation levels. In addition, the design of modified differentiable saturation function will help to construct a hierarchical global convergence strategy to improve the robustness of control design scheme. Through calculation of relevant inequalities, time derivative of boundary surface and simple Lyapunov function,saturation reduction analysis and convergence analysis are carried out, and then a set of explicit parameter conditions are provided to ensure global asymptotic stability in the closed-loop systems. Finally, a simplified system of the mechanical model is presented to validate the effectiveness of the proposed method.展开更多
Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimental...Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity.展开更多
Mathematical modelling has been extensively used to measure intervention strategies for the control of contagious conditions.Alignment between different models is pivotal for furnishing strong substantiation for polic...Mathematical modelling has been extensively used to measure intervention strategies for the control of contagious conditions.Alignment between different models is pivotal for furnishing strong substantiation for policymakers because the differences in model features can impact their prognostications.Mathematical modelling has been widely used in order to better understand the transmission,treatment,and prevention of infectious diseases.Herein,we study the dynamics of a human immunodeficiency virus(HIV)infection model with four variables:S(t),I(t),C(t),and A(t)the susceptible individuals;HIV infected individuals(with no clinical symptoms of AIDS);HIV infected individuals(under ART with a viral load remaining low),and HIV infected individuals with two different incidence functions(bilinear and saturated incidence functions).A novel numerical scheme called the continuous Galerkin-Petrov method is implemented for the solution of themodel.The influence of different clinical parameters on the dynamical behavior of S(t),I(t),C(t)and A(t)is described and analyzed.All the results are depicted graphically.On the other hand,we explore the time-dependent movement of nanofluid in porous media on an extending sheet under the influence of thermal radiation,heat flux,hall impact,variable heat source,and nanomaterial.The flow is considered to be 2D,boundary layer,viscous,incompressible,laminar,and unsteady.Sufficient transformations turn governing connected PDEs intoODEs,which are solved using the proposed scheme.To justify the envisaged problem,a comparison of the current work with previous literature is presented.展开更多
Frost heave in seasonally frozen regions is a one-dimensional process that could severely damage infrastructure subgrades.Stress state,temperature and water migration are important factors for frost heave.This work in...Frost heave in seasonally frozen regions is a one-dimensional process that could severely damage infrastructure subgrades.Stress state,temperature and water migration are important factors for frost heave.This work investigated the effects of soil temperature and volumetric water content on the transient frost heave ratio during the freezing of saturated silty clay in an open system and analyzed the relationships between the transient frost heave ratio and freezing rate and between temperature gradient and frost heave rate.The results show that the frost heave ratio,frost heave rate,and freezing rate are positively correlated with the temperature gradient since the temperature gradient drives the water migration during freezing,indicating the transient temperature gradient could be used to evaluate the frost heave of saturated silty clay.The transient freezing rate and transient frost heave ratio are logarithmically related to the transient frost heave ratio and transient temperature gradient,respectively.The effects of transient temperature gradient on frost heave are the principal mechanism responsible for different frost heave characteristics and uneven frost heave along a subgrade of the same soil type.展开更多
Three high-temperature resistant polymeric additives for water-based drilling fluids are designed and developed:weakly cross-linked zwitterionic polymer fluid loss reducer(WCZ),flexible polymer microsphere nano-pluggi...Three high-temperature resistant polymeric additives for water-based drilling fluids are designed and developed:weakly cross-linked zwitterionic polymer fluid loss reducer(WCZ),flexible polymer microsphere nano-plugging agent(FPM)and comb-structure polymeric lubricant(CSP).A high-temperature resistant and high-density polymeric saturated brine-based drilling fluid was developed for deep drilling.The WCZ has a good anti-polyelectrolyte effect and exhibits the API fluid loss less than 8 mL after aging in saturated salt environment at 200°C.The FPM can reduce the fluid loss by improving the quality of the mud cake and has a good plugging effect on nano-scale pores/fractures.The CSP,with a weight average molecular weight of 4804,has multiple polar adsorption sites and exhibits excellent lubricating performance under high temperature and high salt conditions.The developed drilling fluid system with a density of 2.0 g/cm^(3)has good rheological properties.It shows a fluid loss less than 15 mL at 200°C and high pressure,a sedimentation factor(SF)smaller than 0.52 after standing at high temperature for 5 d,and a rolling recovery of hydratable drill cuttings similar to oil-based drilling fluid.Besides,it has good plugging and lubricating performance.展开更多
Hydraulic conductivity is the ability of a porous media to transfer water through its pore matrix. That is a key parameter for the design and analysis of soil fluid associated structures and issues. This paper present...Hydraulic conductivity is the ability of a porous media to transfer water through its pore matrix. That is a key parameter for the design and analysis of soil fluid associated structures and issues. This paper presents the test results of the vertical hydraulic conductivity k<sub>v</sub><sub> </sub>carried out on one poorly graded sand and three gap graded gravely sand. It was found that the vertical hydraulic conductivity of saturated soil depends on the grain size distribution curve, on the initial relative density of the soil. Compilation of these current test results and other test results published, shows that the common approaches predict well to some extent the vertical hydraulic conductivity k<sub>v</sub> for the poorly graded sand materials and underestimate the k<sub>v</sub> values for gap graded gravely sand materials. Therefore, new approaches are developed for the prediction of the vertical hydraulic conductivity in saturated poorly graded sand and gap graded gravely sand. The derived results from the new approaches lie in the range of the recommended values by (EAU 2012) and (NAVFAC DM 7 1974).展开更多
Shale porosity measurements have crucial scientific and economical applications in unconventionalpetroleum systems. As a standard technique, liquid saturation methods, including water saturation (WS)and oil saturation...Shale porosity measurements have crucial scientific and economical applications in unconventionalpetroleum systems. As a standard technique, liquid saturation methods, including water saturation (WS)and oil saturation (OS), have been widely used to measure the porosity of many rock types. For clay-richshale reservoirs with high organic matter content, it is well known that the WS method may cause clayswelling and induce structural changes in the pore system. The OS method affects the accuracy ofporosity measurements because of some of the oil being dissolved by kerogen within the shale;however,this has not received sufficient research attention. In this study, we compare the previously reported andnewly tested OS porosities with helium (He) expansion porosity. Results show that OS porosity generallyexceeds the He porosity. Furthermore, the higher the total organic carbon (TOC) content and lower thematurity of shale, the greater the difference between the OS and helium porosities. When using the OSmethod, the effect of kerogen-dissolved oil causes an overestimation of the shale porosity by ~30%. To thebest of our knowledge, this is the first time to note the kerogen-dissolve oil effects on OS porosity. Herein,we propose a new, simple, and effective correction method for estimating OS porosity that involvessubtracting the kerogen-dissolved oil content from raw OS porosity. In addition, the quantification modelof kerogen-dissolved oil capacity is established, taking into account the abundance and maturity oforganic matter. Taking the He porosity as the benchmark, the absolute error of the corrected OS porositydoes not exceed 1% and the average relative error is only ~10%. The obtained results can help improve theaccuracy of shale porosity evaluation methods.展开更多
AIM: To investigate how the saturated and unsaturated fatty acid composition influences the susceptibility of developing acute pancreatitis. METHODS: Primary pancreatic acinar cells were treated with low and high conc...AIM: To investigate how the saturated and unsaturated fatty acid composition influences the susceptibility of developing acute pancreatitis. METHODS: Primary pancreatic acinar cells were treated with low and high concentrations of different saturated and unsaturated fatty acids, and changes in the cytosolic Ca2+ signal and the expression of protein kinase C(PKC) were measured after treatment. RESULTS: Unsaturated fatty acids at high concentrations, including oleic acid, linoleic acid, palmitoleic acid, docosahexaenoic acid, and arachidonic acid, induced a persistent rise in cytosolic Ca2+ concentrations in acinar cells. Unsaturated fatty acids at low concentrations and saturated fatty acids, including palmitic acid, stearic acid, and triglycerides, at low and high concentrations were unable to induce a rise in Ca2+ concentrations in acinar cells. Unsaturated fatty acids at high concentrations but not saturated fatty acids induced intra-acinar cell trypsin activation and cell damage and increased PKC expression.CONCLUSION: At sufficiently high concentrations, unsaturated fatty acids were able to induce acinar cells injury and promote the development of pancreatitis. Unsaturated fatty acids may play a distinctive role in the pathogenesis of pancreatitis through the activation of PKC family members.展开更多
Dietary polyunsaturated fatty acids (PUFAs), which are abundant in marine fish oils, have recently received global attention for their prominent anti-obesogenic effects. Among PUFAs, eicosapentaenoic acid (EPA; 20...Dietary polyunsaturated fatty acids (PUFAs), which are abundant in marine fish oils, have recently received global attention for their prominent anti-obesogenic effects. Among PUFAs, eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3), which are n-3 long-chain PUFAs widely referred to as omega-3 oils, were reported to prevent the development of obesity in rodents and humans. In the present study, we evaluated the anti-obesity effects of microalgal oil on high-fat induced obese C57BL/6 mice, compared with commercial omega-3 fish oil and vegetable corn oil. Microalgal oil is an inherent mixture of several PUFAs, including EPA, DHA and other fatty acids produced from a marine microalgal strain of Thraustochytriidae sp. derived mutant. It was found to contain more PUFAs (〉80%) and more omega-3 oils than commercial omega-3 fish oil (PUFAs 〉31%) and corn oil (PUFAs 59%). All three types of oils induced weight loss in high-fat-induced obese mice, with the loss induced by microalgal oil being most significant at 9 weeks (10% reduction). However, the oils tested did not improve blood lipid levels, although microalgal oil showed an apparent inhibitory effect on lipid accumulation in the liver. These findings may be attributed to the higher PUFA content, including omega-3 oils of microalgal oil than other oils. Collectively, these findings suggest that microalgal oil, derived from Thraustochytriidae sp. derived mutant, is a prominent candidate for replacement of omega-3 fish oils based on its apparent anti-obesity effect in vivo.展开更多
Based on the three-dimensional Gurtin-type variational principle of the incompressible saturated porous media, a one-dimensional mathematical model for dynamics of the saturated poroelastic Timoshenko cantilever beam ...Based on the three-dimensional Gurtin-type variational principle of the incompressible saturated porous media, a one-dimensional mathematical model for dynamics of the saturated poroelastic Timoshenko cantilever beam is established with two assumptions, i.e., the deformation satisfies the classical single phase Timoshenko beam and the movement of the pore fluid is only in the axial direction of the saturated poroelastic beam. Under some special cases, this mathematical model can be degenerated into the Euler-Bernoulli model, the Rayleigh model, and the shear model of the saturated poroelastic beam, respectively. The dynamic and quasi-static behaviors of a saturated poroelastic Timoshenko cantilever beam with an impermeable fixed end and a permeable free end subjected to a step load at its free end are analyzed by the Laplace transform. The variations of the deflections at the beam free end against time are shown in figures. The influences of the interaction coefficient between the pore fluid and the solid skeleton as well as the slenderness ratio of the beam on the dynamic/quasi-static performances of the beam are examined. It is shown that the quasi-static deflections of the saturated poroelastic beam possess a creep behavior similar to that of viscoelastic beams. In dynamic responses, with the increase of the slenderness ratio, the vibration periods and amplitudes of the deflections at the free end increase, and the time needed for deflections approaching to their stationary values also increases. Moreover, with the increase of the interaction coefficient, the vibrations of the beam deflections decay more strongly, and, eventually, the deflections of the saturated poroelastic beam converge to the static deflections of the classic single phase Timoshenko beam.展开更多
Accurate prediction of chemical composition of vacuum gas oil (VGO) is essential for the routine operation of refineries. In this work, a new approach for auto-design of artificial neural networks (ANN) based on a...Accurate prediction of chemical composition of vacuum gas oil (VGO) is essential for the routine operation of refineries. In this work, a new approach for auto-design of artificial neural networks (ANN) based on a genetic algorithm (GA) is developed for predicting VGO saturates. The number of neurons in the hidden layer, the momentum and the learning rates are determined by using the genetic algorithm. The inputs for the artificial neural networks model are five physical properties, namely, average boiling point, density, molecular weight, viscosity and refractive index. It is verified that the genetic algorithm could find the optimal structural parameters and training parameters of ANN. In addition, an artificial neural networks model based on a genetic algorithm was tested and the results indicated that the VGO saturates can be efficiently predicted. Compared with conventional artificial neural networks models, this approach can improve the prediction accuracy.展开更多
Three identical model boxes were made from transparent plexiglass and angle iron. Using the method of sinking water and according to the sedimentary rhythm of saturated calcium carbonate (lime-mud) intercalated with...Three identical model boxes were made from transparent plexiglass and angle iron. Using the method of sinking water and according to the sedimentary rhythm of saturated calcium carbonate (lime-mud) intercalated with cohesive soil, calcites with particle sizes diameters of ≤ 5 μm, 10–15 μm and 23–30 μm as well as cohesive soil were sunk alternatively in water of three boxes to build three test models, each of which has a specific size of calcite. Pore water pressure gauges were buried in lime-mud layers at different depths in each model, and connected with a computer system to collect pore water pressures. By means of soil tests, physical property parameters and plasticity indices (Ip) were obtained for various grain-sized saturated lime-muds. The lime-muds with Ip ranging from 6.3 to 8.5 (lower than 10) are similar to liquid saturated silt in the physical nature, indicating that saturated silt can be liquefied once induced by a strong earthquake. One model cart was pushed quickly along the length direction of the model so that its rigid wheels collided violently with the stone stair, thus generating an artificial earthquake with seismic wave magnitude greater than VI degree. When unidirectional cyclic seismic load of horizontal compression-tension-shear was imposed on the soil layers in the model, enough great pore water pressure has been accumulated within pores of lime-mud, resulting in liquefaction of lime-mud layers. Meanwhile, micro-fractures formed in each soil layer provided channels for liquefaction dewatering, resulting in formation of macroscopic liquefaction deformation, such as liquefied lime-mud volcanoes, liquefied diapir structures, vein-like liquefied structures and liquefied curls, etc. Splendid liquefied lime-mud eruption lasted for two to three hours, which is similar to the sand volcano eruption induced by strong earthquake. However, under the same artificial seismic conditions, development of macroscopic liquefied structures in three experimental models varied in shape, depth and quantity, indicating that excess pore water pressure ratios at initial liquefaction stage and complete liquefaction varied with depth. With size increasing of calcite particle in lime-mud, liquefied depth and deformation extent increase accordingly. The simulation test verifies for the first time that strong earthquakes may cause violent liquefaction of saturated lime-mud composed of micron-size calcite particles, uncovering the puzzled issue whether seafloor lime-mud can be liquefied under strong earthquake. This study not only provides the latest simulation data for explaining the earthquake-induced liquefied deformations of saturated lime-mud and seismic sedimentary events, but also is of great significance for analysis of foundation stability in marine engineering built on the soft calcium carbonate layers in neritic environment.展开更多
AIM: To investigate the effect of different dietary fatty acids on hepatic inflammasome activation.METHODS: Wild-type C57BL/6 mice were fed either a high-fat diet or polyunsaturated fatty acid(PUFA)-enriched diet. Pri...AIM: To investigate the effect of different dietary fatty acids on hepatic inflammasome activation.METHODS: Wild-type C57BL/6 mice were fed either a high-fat diet or polyunsaturated fatty acid(PUFA)-enriched diet. Primary hepatocytes were treated with either saturated fatty acids(SFAs) or PUFAs as well as combined with lipopolysaccharide(LPS). The expression of NOD-like receptor protein 3(NLRP3) inflammasome, peroxisome proliferator-activated receptor-γ and nuclear factor-kappa B(NF-κB) was determined by real-time PCR and Western blot. The activity of Caspase-1 and interleukine-1β production were measured.RESULTS: high-fat diet-induced hepatic steatosis was sufficient to induce and activate hepatic NLRP3 inflammasome. SFA palmitic acid(PA) directly activated NLRP3 inflammasome and increased sensitization to LPS-induced inflammasome activation in hepatocytes. In contrast, PUFA docosahexaenoic acid(Dh A) had thepotential to inhibit NLRP3 inflammasome expression in hepatocytes and partly abolished LPS-induced NLRP3 inflammasome activation. Furthermore, a highfat diet increased but PUFA-enriched diet decreased sensitization to LPS-induced hepatic NLRP3 inflammasome activation in vivo. Moreover, PA increased but Dh A decreased phosphorylated NF-κB p65 protein expression in hepatocytes.CONCLUSION:Hepatic NLRP 3 inflammasome activation played an important role in the development of non-alcoholic fatty liver disease. Dietary SFAs and PUFAs oppositely regulated the activity of NLRP3 inflammasome through direct activation or inhibition of NF-κB.展开更多
This study considers the torsional vibration of a pipe pile in a transversely isotropic saturated soil layer. Based on Biot's poroelastic theory and the constitutive relations of the transversely isotropic medium, th...This study considers the torsional vibration of a pipe pile in a transversely isotropic saturated soil layer. Based on Biot's poroelastic theory and the constitutive relations of the transversely isotropic medium, the dynamic governing equations of the outer and inner transversely isotropic saturated soil layers are derived. The Laplace transform is used to solve the governing equations of the outer and inner soil layers. The dynamic torsional response of the pipe pile in the frequency domain is derived utilizing 1D elastic theory and the continuous conditions at the interfaces between the pipe pile and the soils. The time domain solution is obtained by Fourier inverse transform. A parametric study is conducted to demonstrate the influence of the anisotropies of the outer and inner soil on the torsional dynamic response of the pipe pile.展开更多
Based on the theory of porous media, a general Gurtin variational principle for the initial boundary value problem of dynamical response of fluid-saturated elastic porous media is developed by assuming infinitesimal d...Based on the theory of porous media, a general Gurtin variational principle for the initial boundary value problem of dynamical response of fluid-saturated elastic porous media is developed by assuming infinitesimal deformation and incompressible constituents of the solid and fluid phase. The finite element formulation based on this variational principle is also derived. As the functional of the variational principle is a spatial integral of the convolution formulation, the general finite element discretization in space results in symmetrical differential-integral equations in the time domain. In some situations, the differential-integral equations can be reduced to symmetrical differential equations and, as a numerical example, it is employed to analyze the reflection of one-dimensional longitudinal wave in a fluid-saturated porous solid. The numerical results can provide further understanding of the wave propagation in porous media.展开更多
In order to analyze the impact of stretching-segment on the saturated flow rate of signalized intersection approach, an improved cellular automation model was proposed to estimate its saturated flow rate. The NaSch mo...In order to analyze the impact of stretching-segment on the saturated flow rate of signalized intersection approach, an improved cellular automation model was proposed to estimate its saturated flow rate. The NaSch model was improved by adding different slow probabilities, turning deceleration rules and modified lane changing rules. The relationship between the saturated flow rate of stretching-segments and adjacent lanes was tested in numerical simulation. The length of stretching-segment, cycle length and green time were selected as impact factors of the cellular automation model. The simulation result indicates that the geometrics design of stretching-segment and the traffic signal timing scenario have major effects on the saturated flow rate of the intersection approach. The saturated flow rate will continually increase with increasing stretching-segment length until it reaches a threshold. After reaching the threshold, the stretching-segment can be treated as a separate lane. The green time is approximately linearly related to the threshold length of the stretching-segment. An optimum cycle length exists when the length of the stretching-segment is not long enough, and it is approximately linearly related to the length of stretching-segment.展开更多
In order to investigate the influence of the vertical vibration loading on the liquefaction of saturated sand,one dimensional model for the saturated sand with a vertical vibration is presented based on the two phase ...In order to investigate the influence of the vertical vibration loading on the liquefaction of saturated sand,one dimensional model for the saturated sand with a vertical vibration is presented based on the two phase continuous media theory.The development of the liquefaction and the lique- faction region are analyzed.It is shown that the vertical vibration loading could induce liquefaction. The rate of the liquefaction increases with the increase of the initial limit strain or initial porosity or amplitude and frequency of loading,and increases with the decrease of the permeability or initial modulus.It is shown also that there is a phase lag in the sand column.When the sand permeability distribution is non-uniform,the pore pressure and the strain will rise sharply where the permeability is the smallest,and fracture might be induced.With the development of liquefaction,the strength of the soil foundation becomes smaller and smaller.In the limiting case,landslides or debris flows could occur.展开更多
The Blot's wave equations of transversely isotropic saturated poroelastic media excited hy non-axisymmetrical harmonic source were solved by means of Fourier expansion and Hankel transform. Then the components of ...The Blot's wave equations of transversely isotropic saturated poroelastic media excited hy non-axisymmetrical harmonic source were solved by means of Fourier expansion and Hankel transform. Then the components of total stress in porous media are expressed with the solutions of Biot's wave equations. The method of research on non-axisymmetrical dynamic response of saturated porous media is discussed, and a numerical result is presented.展开更多
The seismic response characteristics of underground structures in saturated soils are investigated.A fully fluid-solid coupling dynamic model is developed and implemented into ABAQUS with a user-defined element to sim...The seismic response characteristics of underground structures in saturated soils are investigated.A fully fluid-solid coupling dynamic model is developed and implemented into ABAQUS with a user-defined element to simulate the dynamic behavior of saturated soils.The accuracy of the model is validated using a classic example in literature.The performance of the model is verified by its application on simulating the seismic response characteristics of a subway station built in saturated soils.The merits of the model are demonstrated by comparing the difference of the seismic response of an underground structure in saturated soils between using the fully coupling model and a single-phase medium model.The study finds that the fully coupling model developed herein can simulate the dynamic response characteristics of the underground structures in saturated soils with high accuracy.The seismic response of the underground structure tends to be underestimated by using the single-phase medium model compared with using the fully coupling model,which provides a weaker confining action to the underground structure.展开更多
Akebia trifoliata subsp.australis is a well-known medicinal and potential woody oil plant in China.The limited genetic information available for A.trifoliata subsp.australis has hindered its exploitation.Here,a high-q...Akebia trifoliata subsp.australis is a well-known medicinal and potential woody oil plant in China.The limited genetic information available for A.trifoliata subsp.australis has hindered its exploitation.Here,a high-quality chromosomelevel genome sequence of A.trifoliata subsp.australis is reported.The de novo genome assembly of 682.14 Mb was generated with a scaffold N50 of 43.11 Mb.The genome includes 25,598 protein-coding genes,and 71.18%(485.55 Mb)of the assembled sequences were identi fi ed as repetitive sequences.An ongoing massive burst of long terminal repeat(LTR)insertions,which occurred~1.0 million years ago,has contributed a large proportion of LTRs in the genome of A.trifoliata subsp.australis.Phylogenetic analysis shows that A.trifoliata subsp.australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera,which supports the well-established hypothesis of a close relationship between basal eudicot species.The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and fi-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A.trifoliata subsp.australis.Furthermore,the acyl-ACP desaturase gene family,including 12 stearoyl-acyl-carrier protein desaturase(SAD)genes,has expanded exclusively.A combined transcriptome and fatty-acid analysis of seeds at fi ve developmental stages revealed that homologs of SADs,acyl-lipid desaturase omega fatty acid desaturases(FADs),and oleosins were highly expressed,consistent with the rapid increase in the content of fatty acids,especially unsaturated fatty acids.The genomic sequences of A.trifoliata subsp.australis will be a valuable resource for comparative genomic analyses and molecular breeding.展开更多
基金supported in part by the National Natural Science Foundation of China (62203178, U1913602, 61936004)the National Key Rsearch and Development Program of China (2021ZD0201300)+3 种基金the China Postdoctoral Science Foundation (2021TQ0116)the Innovation Group Project of the National Natural Science Foundation of China (61821003)the Technology Innovation Project of Hubei Province of China (2019AE A171)the 111 Project on Computational Intelligence and Intelligent Control (B18024)。
文摘This study addresses the problem of global asymptotic stability for uncertain complex cascade systems composed of multiple integrator systems and non-strict feedforward nonlinear systems. To tackle the complexity inherent in such structures, a novel nested saturated control design is proposed that incorporates both constant saturation levels and state-dependent saturation levels. Specifically, a modified differentiable saturation function is proposed to facilitate the saturation reduction analysis of the uncertain complex cascade systems under the presence of mixed saturation levels. In addition, the design of modified differentiable saturation function will help to construct a hierarchical global convergence strategy to improve the robustness of control design scheme. Through calculation of relevant inequalities, time derivative of boundary surface and simple Lyapunov function,saturation reduction analysis and convergence analysis are carried out, and then a set of explicit parameter conditions are provided to ensure global asymptotic stability in the closed-loop systems. Finally, a simplified system of the mechanical model is presented to validate the effectiveness of the proposed method.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61571018 and 61531003).
文摘Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity.
文摘Mathematical modelling has been extensively used to measure intervention strategies for the control of contagious conditions.Alignment between different models is pivotal for furnishing strong substantiation for policymakers because the differences in model features can impact their prognostications.Mathematical modelling has been widely used in order to better understand the transmission,treatment,and prevention of infectious diseases.Herein,we study the dynamics of a human immunodeficiency virus(HIV)infection model with four variables:S(t),I(t),C(t),and A(t)the susceptible individuals;HIV infected individuals(with no clinical symptoms of AIDS);HIV infected individuals(under ART with a viral load remaining low),and HIV infected individuals with two different incidence functions(bilinear and saturated incidence functions).A novel numerical scheme called the continuous Galerkin-Petrov method is implemented for the solution of themodel.The influence of different clinical parameters on the dynamical behavior of S(t),I(t),C(t)and A(t)is described and analyzed.All the results are depicted graphically.On the other hand,we explore the time-dependent movement of nanofluid in porous media on an extending sheet under the influence of thermal radiation,heat flux,hall impact,variable heat source,and nanomaterial.The flow is considered to be 2D,boundary layer,viscous,incompressible,laminar,and unsteady.Sufficient transformations turn governing connected PDEs intoODEs,which are solved using the proposed scheme.To justify the envisaged problem,a comparison of the current work with previous literature is presented.
基金supported by the National Natural Science Foundation of China(No.51808128)the Natural Science Foundation of Fujian Province(No.2022J01091)。
文摘Frost heave in seasonally frozen regions is a one-dimensional process that could severely damage infrastructure subgrades.Stress state,temperature and water migration are important factors for frost heave.This work investigated the effects of soil temperature and volumetric water content on the transient frost heave ratio during the freezing of saturated silty clay in an open system and analyzed the relationships between the transient frost heave ratio and freezing rate and between temperature gradient and frost heave rate.The results show that the frost heave ratio,frost heave rate,and freezing rate are positively correlated with the temperature gradient since the temperature gradient drives the water migration during freezing,indicating the transient temperature gradient could be used to evaluate the frost heave of saturated silty clay.The transient freezing rate and transient frost heave ratio are logarithmically related to the transient frost heave ratio and transient temperature gradient,respectively.The effects of transient temperature gradient on frost heave are the principal mechanism responsible for different frost heave characteristics and uneven frost heave along a subgrade of the same soil type.
基金Supported by the National Natural Science Foundation of China(52288101).
文摘Three high-temperature resistant polymeric additives for water-based drilling fluids are designed and developed:weakly cross-linked zwitterionic polymer fluid loss reducer(WCZ),flexible polymer microsphere nano-plugging agent(FPM)and comb-structure polymeric lubricant(CSP).A high-temperature resistant and high-density polymeric saturated brine-based drilling fluid was developed for deep drilling.The WCZ has a good anti-polyelectrolyte effect and exhibits the API fluid loss less than 8 mL after aging in saturated salt environment at 200°C.The FPM can reduce the fluid loss by improving the quality of the mud cake and has a good plugging effect on nano-scale pores/fractures.The CSP,with a weight average molecular weight of 4804,has multiple polar adsorption sites and exhibits excellent lubricating performance under high temperature and high salt conditions.The developed drilling fluid system with a density of 2.0 g/cm^(3)has good rheological properties.It shows a fluid loss less than 15 mL at 200°C and high pressure,a sedimentation factor(SF)smaller than 0.52 after standing at high temperature for 5 d,and a rolling recovery of hydratable drill cuttings similar to oil-based drilling fluid.Besides,it has good plugging and lubricating performance.
文摘Hydraulic conductivity is the ability of a porous media to transfer water through its pore matrix. That is a key parameter for the design and analysis of soil fluid associated structures and issues. This paper presents the test results of the vertical hydraulic conductivity k<sub>v</sub><sub> </sub>carried out on one poorly graded sand and three gap graded gravely sand. It was found that the vertical hydraulic conductivity of saturated soil depends on the grain size distribution curve, on the initial relative density of the soil. Compilation of these current test results and other test results published, shows that the common approaches predict well to some extent the vertical hydraulic conductivity k<sub>v</sub> for the poorly graded sand materials and underestimate the k<sub>v</sub> values for gap graded gravely sand materials. Therefore, new approaches are developed for the prediction of the vertical hydraulic conductivity in saturated poorly graded sand and gap graded gravely sand. The derived results from the new approaches lie in the range of the recommended values by (EAU 2012) and (NAVFAC DM 7 1974).
基金funded by the National Natural Science Foundation of China(42102154,41922015,42072147)Postdoctoral Research Foundation of China(2021M690168)+4 种基金Postdoctoral Innovative Talent Support Program of Shandong Province(SDBX2021004)Open Fund of Shaanxi Key Laboratory of Petroleum Accumulation Geology(PAG-2021-02)Open Funds of the Key Laboratory of Tectonics and Petroleum Resources(China University of Geosciences)(TPR-2021-02)Fundamental Research Funds for the Central Universities(20CX06085A)Qingdao Postdoctoral(ZX20210070).
文摘Shale porosity measurements have crucial scientific and economical applications in unconventionalpetroleum systems. As a standard technique, liquid saturation methods, including water saturation (WS)and oil saturation (OS), have been widely used to measure the porosity of many rock types. For clay-richshale reservoirs with high organic matter content, it is well known that the WS method may cause clayswelling and induce structural changes in the pore system. The OS method affects the accuracy ofporosity measurements because of some of the oil being dissolved by kerogen within the shale;however,this has not received sufficient research attention. In this study, we compare the previously reported andnewly tested OS porosities with helium (He) expansion porosity. Results show that OS porosity generallyexceeds the He porosity. Furthermore, the higher the total organic carbon (TOC) content and lower thematurity of shale, the greater the difference between the OS and helium porosities. When using the OSmethod, the effect of kerogen-dissolved oil causes an overestimation of the shale porosity by ~30%. To thebest of our knowledge, this is the first time to note the kerogen-dissolve oil effects on OS porosity. Herein,we propose a new, simple, and effective correction method for estimating OS porosity that involvessubtracting the kerogen-dissolved oil content from raw OS porosity. In addition, the quantification modelof kerogen-dissolved oil capacity is established, taking into account the abundance and maturity oforganic matter. Taking the He porosity as the benchmark, the absolute error of the corrected OS porositydoes not exceed 1% and the average relative error is only ~10%. The obtained results can help improve theaccuracy of shale porosity evaluation methods.
文摘AIM: To investigate how the saturated and unsaturated fatty acid composition influences the susceptibility of developing acute pancreatitis. METHODS: Primary pancreatic acinar cells were treated with low and high concentrations of different saturated and unsaturated fatty acids, and changes in the cytosolic Ca2+ signal and the expression of protein kinase C(PKC) were measured after treatment. RESULTS: Unsaturated fatty acids at high concentrations, including oleic acid, linoleic acid, palmitoleic acid, docosahexaenoic acid, and arachidonic acid, induced a persistent rise in cytosolic Ca2+ concentrations in acinar cells. Unsaturated fatty acids at low concentrations and saturated fatty acids, including palmitic acid, stearic acid, and triglycerides, at low and high concentrations were unable to induce a rise in Ca2+ concentrations in acinar cells. Unsaturated fatty acids at high concentrations but not saturated fatty acids induced intra-acinar cell trypsin activation and cell damage and increased PKC expression.CONCLUSION: At sufficiently high concentrations, unsaturated fatty acids were able to induce acinar cells injury and promote the development of pancreatitis. Unsaturated fatty acids may play a distinctive role in the pathogenesis of pancreatitis through the activation of PKC family members.
基金supported by a grant from the KRIBB Research Initiative Program(KGM2211531)supported by Priority Research Centers Program through NRF funded by the Ministry of Education,Science and Technology (2015R1A6A1A04020885)
文摘Dietary polyunsaturated fatty acids (PUFAs), which are abundant in marine fish oils, have recently received global attention for their prominent anti-obesogenic effects. Among PUFAs, eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3), which are n-3 long-chain PUFAs widely referred to as omega-3 oils, were reported to prevent the development of obesity in rodents and humans. In the present study, we evaluated the anti-obesity effects of microalgal oil on high-fat induced obese C57BL/6 mice, compared with commercial omega-3 fish oil and vegetable corn oil. Microalgal oil is an inherent mixture of several PUFAs, including EPA, DHA and other fatty acids produced from a marine microalgal strain of Thraustochytriidae sp. derived mutant. It was found to contain more PUFAs (〉80%) and more omega-3 oils than commercial omega-3 fish oil (PUFAs 〉31%) and corn oil (PUFAs 59%). All three types of oils induced weight loss in high-fat-induced obese mice, with the loss induced by microalgal oil being most significant at 9 weeks (10% reduction). However, the oils tested did not improve blood lipid levels, although microalgal oil showed an apparent inhibitory effect on lipid accumulation in the liver. These findings may be attributed to the higher PUFA content, including omega-3 oils of microalgal oil than other oils. Collectively, these findings suggest that microalgal oil, derived from Thraustochytriidae sp. derived mutant, is a prominent candidate for replacement of omega-3 fish oils based on its apparent anti-obesity effect in vivo.
基金Project supported by the National Natural Science Foundation of China (No. 10872124)
文摘Based on the three-dimensional Gurtin-type variational principle of the incompressible saturated porous media, a one-dimensional mathematical model for dynamics of the saturated poroelastic Timoshenko cantilever beam is established with two assumptions, i.e., the deformation satisfies the classical single phase Timoshenko beam and the movement of the pore fluid is only in the axial direction of the saturated poroelastic beam. Under some special cases, this mathematical model can be degenerated into the Euler-Bernoulli model, the Rayleigh model, and the shear model of the saturated poroelastic beam, respectively. The dynamic and quasi-static behaviors of a saturated poroelastic Timoshenko cantilever beam with an impermeable fixed end and a permeable free end subjected to a step load at its free end are analyzed by the Laplace transform. The variations of the deflections at the beam free end against time are shown in figures. The influences of the interaction coefficient between the pore fluid and the solid skeleton as well as the slenderness ratio of the beam on the dynamic/quasi-static performances of the beam are examined. It is shown that the quasi-static deflections of the saturated poroelastic beam possess a creep behavior similar to that of viscoelastic beams. In dynamic responses, with the increase of the slenderness ratio, the vibration periods and amplitudes of the deflections at the free end increase, and the time needed for deflections approaching to their stationary values also increases. Moreover, with the increase of the interaction coefficient, the vibrations of the beam deflections decay more strongly, and, eventually, the deflections of the saturated poroelastic beam converge to the static deflections of the classic single phase Timoshenko beam.
文摘Accurate prediction of chemical composition of vacuum gas oil (VGO) is essential for the routine operation of refineries. In this work, a new approach for auto-design of artificial neural networks (ANN) based on a genetic algorithm (GA) is developed for predicting VGO saturates. The number of neurons in the hidden layer, the momentum and the learning rates are determined by using the genetic algorithm. The inputs for the artificial neural networks model are five physical properties, namely, average boiling point, density, molecular weight, viscosity and refractive index. It is verified that the genetic algorithm could find the optimal structural parameters and training parameters of ANN. In addition, an artificial neural networks model based on a genetic algorithm was tested and the results indicated that the VGO saturates can be efficiently predicted. Compared with conventional artificial neural networks models, this approach can improve the prediction accuracy.
基金supported by the National Natural Science Foundation of China(NSFC-41272066)the Program for Changjiang Scholars & Innovative Research Team of the University of China(IRT-13075)
文摘Three identical model boxes were made from transparent plexiglass and angle iron. Using the method of sinking water and according to the sedimentary rhythm of saturated calcium carbonate (lime-mud) intercalated with cohesive soil, calcites with particle sizes diameters of ≤ 5 μm, 10–15 μm and 23–30 μm as well as cohesive soil were sunk alternatively in water of three boxes to build three test models, each of which has a specific size of calcite. Pore water pressure gauges were buried in lime-mud layers at different depths in each model, and connected with a computer system to collect pore water pressures. By means of soil tests, physical property parameters and plasticity indices (Ip) were obtained for various grain-sized saturated lime-muds. The lime-muds with Ip ranging from 6.3 to 8.5 (lower than 10) are similar to liquid saturated silt in the physical nature, indicating that saturated silt can be liquefied once induced by a strong earthquake. One model cart was pushed quickly along the length direction of the model so that its rigid wheels collided violently with the stone stair, thus generating an artificial earthquake with seismic wave magnitude greater than VI degree. When unidirectional cyclic seismic load of horizontal compression-tension-shear was imposed on the soil layers in the model, enough great pore water pressure has been accumulated within pores of lime-mud, resulting in liquefaction of lime-mud layers. Meanwhile, micro-fractures formed in each soil layer provided channels for liquefaction dewatering, resulting in formation of macroscopic liquefaction deformation, such as liquefied lime-mud volcanoes, liquefied diapir structures, vein-like liquefied structures and liquefied curls, etc. Splendid liquefied lime-mud eruption lasted for two to three hours, which is similar to the sand volcano eruption induced by strong earthquake. However, under the same artificial seismic conditions, development of macroscopic liquefied structures in three experimental models varied in shape, depth and quantity, indicating that excess pore water pressure ratios at initial liquefaction stage and complete liquefaction varied with depth. With size increasing of calcite particle in lime-mud, liquefied depth and deformation extent increase accordingly. The simulation test verifies for the first time that strong earthquakes may cause violent liquefaction of saturated lime-mud composed of micron-size calcite particles, uncovering the puzzled issue whether seafloor lime-mud can be liquefied under strong earthquake. This study not only provides the latest simulation data for explaining the earthquake-induced liquefied deformations of saturated lime-mud and seismic sedimentary events, but also is of great significance for analysis of foundation stability in marine engineering built on the soft calcium carbonate layers in neritic environment.
基金Supported by The National Natural Science Foundation of ChinaNO.81170374 and NO.81470842 to Hua J
文摘AIM: To investigate the effect of different dietary fatty acids on hepatic inflammasome activation.METHODS: Wild-type C57BL/6 mice were fed either a high-fat diet or polyunsaturated fatty acid(PUFA)-enriched diet. Primary hepatocytes were treated with either saturated fatty acids(SFAs) or PUFAs as well as combined with lipopolysaccharide(LPS). The expression of NOD-like receptor protein 3(NLRP3) inflammasome, peroxisome proliferator-activated receptor-γ and nuclear factor-kappa B(NF-κB) was determined by real-time PCR and Western blot. The activity of Caspase-1 and interleukine-1β production were measured.RESULTS: high-fat diet-induced hepatic steatosis was sufficient to induce and activate hepatic NLRP3 inflammasome. SFA palmitic acid(PA) directly activated NLRP3 inflammasome and increased sensitization to LPS-induced inflammasome activation in hepatocytes. In contrast, PUFA docosahexaenoic acid(Dh A) had thepotential to inhibit NLRP3 inflammasome expression in hepatocytes and partly abolished LPS-induced NLRP3 inflammasome activation. Furthermore, a highfat diet increased but PUFA-enriched diet decreased sensitization to LPS-induced hepatic NLRP3 inflammasome activation in vivo. Moreover, PA increased but Dh A decreased phosphorylated NF-κB p65 protein expression in hepatocytes.CONCLUSION:Hepatic NLRP 3 inflammasome activation played an important role in the development of non-alcoholic fatty liver disease. Dietary SFAs and PUFAs oppositely regulated the activity of NLRP3 inflammasome through direct activation or inhibition of NF-κB.
基金The 111 Project under Grant No.B13024the National Natural Science Foundation of China under Grant Nos.U1134207 and 51378177the Program for New Century Excellent Talents in University under Grant No.NCET-12-0843
文摘This study considers the torsional vibration of a pipe pile in a transversely isotropic saturated soil layer. Based on Biot's poroelastic theory and the constitutive relations of the transversely isotropic medium, the dynamic governing equations of the outer and inner transversely isotropic saturated soil layers are derived. The Laplace transform is used to solve the governing equations of the outer and inner soil layers. The dynamic torsional response of the pipe pile in the frequency domain is derived utilizing 1D elastic theory and the continuous conditions at the interfaces between the pipe pile and the soils. The time domain solution is obtained by Fourier inverse transform. A parametric study is conducted to demonstrate the influence of the anisotropies of the outer and inner soil on the torsional dynamic response of the pipe pile.
基金Project supported by the National Nattural Science Foundation of China(No.10272070)
文摘Based on the theory of porous media, a general Gurtin variational principle for the initial boundary value problem of dynamical response of fluid-saturated elastic porous media is developed by assuming infinitesimal deformation and incompressible constituents of the solid and fluid phase. The finite element formulation based on this variational principle is also derived. As the functional of the variational principle is a spatial integral of the convolution formulation, the general finite element discretization in space results in symmetrical differential-integral equations in the time domain. In some situations, the differential-integral equations can be reduced to symmetrical differential equations and, as a numerical example, it is employed to analyze the reflection of one-dimensional longitudinal wave in a fluid-saturated porous solid. The numerical results can provide further understanding of the wave propagation in porous media.
基金Projects(50422283,51208054) supported by the National Natural Science Foundation of China
文摘In order to analyze the impact of stretching-segment on the saturated flow rate of signalized intersection approach, an improved cellular automation model was proposed to estimate its saturated flow rate. The NaSch model was improved by adding different slow probabilities, turning deceleration rules and modified lane changing rules. The relationship between the saturated flow rate of stretching-segments and adjacent lanes was tested in numerical simulation. The length of stretching-segment, cycle length and green time were selected as impact factors of the cellular automation model. The simulation result indicates that the geometrics design of stretching-segment and the traffic signal timing scenario have major effects on the saturated flow rate of the intersection approach. The saturated flow rate will continually increase with increasing stretching-segment length until it reaches a threshold. After reaching the threshold, the stretching-segment can be treated as a separate lane. The green time is approximately linearly related to the threshold length of the stretching-segment. An optimum cycle length exists when the length of the stretching-segment is not long enough, and it is approximately linearly related to the length of stretching-segment.
基金The project supported by the National Natural Science Foundation of China(40025103,10202024)
文摘In order to investigate the influence of the vertical vibration loading on the liquefaction of saturated sand,one dimensional model for the saturated sand with a vertical vibration is presented based on the two phase continuous media theory.The development of the liquefaction and the lique- faction region are analyzed.It is shown that the vertical vibration loading could induce liquefaction. The rate of the liquefaction increases with the increase of the initial limit strain or initial porosity or amplitude and frequency of loading,and increases with the decrease of the permeability or initial modulus.It is shown also that there is a phase lag in the sand column.When the sand permeability distribution is non-uniform,the pore pressure and the strain will rise sharply where the permeability is the smallest,and fracture might be induced.With the development of liquefaction,the strength of the soil foundation becomes smaller and smaller.In the limiting case,landslides or debris flows could occur.
文摘The Blot's wave equations of transversely isotropic saturated poroelastic media excited hy non-axisymmetrical harmonic source were solved by means of Fourier expansion and Hankel transform. Then the components of total stress in porous media are expressed with the solutions of Biot's wave equations. The method of research on non-axisymmetrical dynamic response of saturated porous media is discussed, and a numerical result is presented.
基金National Natural Science Foundation of People’s Republic of China under Grant Nos.51178011 and 51778386the Key Fundamental Study Development Project of People’s Republic of China under Grant No.2011CB013602。
文摘The seismic response characteristics of underground structures in saturated soils are investigated.A fully fluid-solid coupling dynamic model is developed and implemented into ABAQUS with a user-defined element to simulate the dynamic behavior of saturated soils.The accuracy of the model is validated using a classic example in literature.The performance of the model is verified by its application on simulating the seismic response characteristics of a subway station built in saturated soils.The merits of the model are demonstrated by comparing the difference of the seismic response of an underground structure in saturated soils between using the fully coupling model and a single-phase medium model.The study finds that the fully coupling model developed herein can simulate the dynamic response characteristics of the underground structures in saturated soils with high accuracy.The seismic response of the underground structure tends to be underestimated by using the single-phase medium model compared with using the fully coupling model,which provides a weaker confining action to the underground structure.
基金the Natural Science Foundation of Hunan Province(2019JJ50475)Key Scientific Research Projects of Hunan Education Department(18A448)Foundation of Hunan Double First-rate Discipline Construction Pr ojects of Bioengineering and Key Lab oratory of Resea rch and Utilizati on of Ethnomedicinal Plant Resources of Hunan Province,and the National Science Foundation(81874334).
文摘Akebia trifoliata subsp.australis is a well-known medicinal and potential woody oil plant in China.The limited genetic information available for A.trifoliata subsp.australis has hindered its exploitation.Here,a high-quality chromosomelevel genome sequence of A.trifoliata subsp.australis is reported.The de novo genome assembly of 682.14 Mb was generated with a scaffold N50 of 43.11 Mb.The genome includes 25,598 protein-coding genes,and 71.18%(485.55 Mb)of the assembled sequences were identi fi ed as repetitive sequences.An ongoing massive burst of long terminal repeat(LTR)insertions,which occurred~1.0 million years ago,has contributed a large proportion of LTRs in the genome of A.trifoliata subsp.australis.Phylogenetic analysis shows that A.trifoliata subsp.australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera,which supports the well-established hypothesis of a close relationship between basal eudicot species.The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and fi-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A.trifoliata subsp.australis.Furthermore,the acyl-ACP desaturase gene family,including 12 stearoyl-acyl-carrier protein desaturase(SAD)genes,has expanded exclusively.A combined transcriptome and fatty-acid analysis of seeds at fi ve developmental stages revealed that homologs of SADs,acyl-lipid desaturase omega fatty acid desaturases(FADs),and oleosins were highly expressed,consistent with the rapid increase in the content of fatty acids,especially unsaturated fatty acids.The genomic sequences of A.trifoliata subsp.australis will be a valuable resource for comparative genomic analyses and molecular breeding.