Objective To study effects of saturated hydrogen saline in preventing noise-induced hearing loss. Methods Fifteen guinea pigs were randomly divided into 3 groups (5 each), group one was for control, group two was tr...Objective To study effects of saturated hydrogen saline in preventing noise-induced hearing loss. Methods Fifteen guinea pigs were randomly divided into 3 groups (5 each), group one was for control, group two was treated with normal saline and group three was treated with saturated hydrogen saline, which was given intraperitoneally at 1 hour before noise exposure at 1 ml/100 g. One hundred rounds of impulse noise ( 157 dB SPL peak) were delivered as noise exposure. Immediately after exposure to impulse noise and on Days 1, 2, 4 and 8 following exposure, auditory brainstem response (ABR) thresholds were measured. Outer hair cell morphological changes and sueeinate dehydrogenase (SDH) activity were examined on Day 8 post-exposure. Results Immediately after noise exposure, ABR thresholds in saturated hydrogen saline treated animals were lower than the non-treated animals (P 〈 0.05 ). Microscopy showed little SDH staining, cell swelling and irregular cell arrangement in the non-treated or normal saline treated animals. Whereas in the saturated hydrogen saline treated animals, there was deep SDH staining with significantly reduced cell loss and more regular cellular arrangement compared to the other two groups. The surviving cells counts was 45.17 ±12.15 for non-treated animals, 44.50 ±10.02 for normal saline treated animals and,116.50±2.38 for animals treated with saturated hydrogen saline. While the count was similar between non-treated and normal saline treated animals, it was significantly higher in saturated sions Intraperitoneal injection of saturated hydrogen saline damage. hydrogen saline treated animals (P 〈 0.05). Concluappears to protect the cochlea against noise-induced damage.展开更多
Background: Hydrogen-rich saline(HRS) has antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hydrogen on hepatic ischemia-reperfusion(I/R) and laparoscopic hepatectomy in sw...Background: Hydrogen-rich saline(HRS) has antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hydrogen on hepatic ischemia-reperfusion(I/R) and laparoscopic hepatectomy in swine. Methods: Twenty-one healthy Bama miniature pigs were randomly divided into the sham group, ischemia-reperfusion injury(IRI) group, HRS-5(5 m L/kg) group, and HRS-10(10 m L/kg) group. HRS was injected through the portal vein 10 min before reperfusion and at postoperative day 1, 2 and 3. The roles of HRS on oxidative stress, inflammatory response and liver regeneration were studied. Results: Compared with the IRI group, HRS treatment attenuated oxidative stress by increasing catalase activity and reducing myeloperoxidase. White blood cells in the HRS-10 group were reduced compared with the IRI group( P < 0.01). In the HRS-10 group, interleukin-1 beta, interleukin-6 and tumor necrosis factor alpha, C-reactive protein and cortisol were downregulated, whereas interleukin-10 was upregulated. In addition, HRS attenuated endothelial cell injury and promoted the secretion of angiogenic cytokines, including vascular endothelial growth factor, angiopoietin-1 and angiopoietin-2. HRS elevated the levels of hepatocyte growth factor, Cyclin D1, proliferating cell nuclear antigen, Ki-67 and reduced the secretion of transforming growth factor-beta. Conclusions: HRS treatment may exert a protective effect against I/R and hepatectomy-induced hepatic damage by reducing oxidative stress, suppressing the inflammatory response and promoting liver regeneration.展开更多
Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich salin...Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspa se-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.展开更多
Exposure of skin to solar ultraviolet(UV) radiation induces photo-damage.Ultraviolet B(UVB) is the major component of UV radiation which induces the production of reactive oxygen species(ROS) and plays an impor-...Exposure of skin to solar ultraviolet(UV) radiation induces photo-damage.Ultraviolet B(UVB) is the major component of UV radiation which induces the production of reactive oxygen species(ROS) and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation.UVB radiation was irradiated on female C57BL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVB radia-tion had a significant affection in tumor necrosis factor alpha,interleukin(IL)-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVB radiation injury,possibly by reducing inflammation and oxidative stress.展开更多
AIM:To investigate the effects of hydrogen-rich saline(HRS)on microglia activation and Sirtuin type 1(Sirt1)in rats with N-methyl-N-nitrosourea(MNU)-induced retinitis pigmentosa(RP).METHODS:Rats were divided...AIM:To investigate the effects of hydrogen-rich saline(HRS)on microglia activation and Sirtuin type 1(Sirt1)in rats with N-methyl-N-nitrosourea(MNU)-induced retinitis pigmentosa(RP).METHODS:Rats were divided into norm(N)group,model(M)group and HRS(H)group.Rats in M and H groups were given saline and HRS respectively prior to and after administration of MNU.At one day(d1)and d3 afterwards,electroretinogram and histological examination were performed to confirm the effects of HRS on retinal function and structure of MNU-induced RP.Immunofluorescence staining of anti-ionized calcium-binding adapter molecule 1(Iba1),a maker of microglia cells,was performed,with quantitative real-time polymerase chain reaction(qRT-PCR)for its m RNA quantification.Moreover,Sirt1 m RNA and protein expression in the retinas were detected by Western blot and qRT-PCR.RESULTS:HRS preserved the retinal function and mitigated the reduction of photoreceptor degeneration in MNU-treated retinas.The presence of microglia cells was somewhat more obvious in H group than that in M group at d1.HRS suppressed the further activation of microglia cells,with the number of microglia cells less than that of M group at d3.Results of qRT-PCR of Iba1 were consistent with those of immunofluorescence staining,with the m RNA expression of Iba1 in H group more intensive than that of M group at d1(P〈0.05),while less than that of M group at d3(P〈0.05).Furthermore,the Sirt1 m RNA and protein expression decreased after MNU administration,while HRS mitigated the MNU-induced downregulation of Sirt1.CONCLUSION:HRS can effectively keep microglia activation induced by MNU to an appropriate extent,while upregulate Sirt1 in MNU-induced RP.展开更多
Objective To explore protective effect of hydrogen - rich saline on liver ischemia reperfusion ( IR) in mice and possible mechanisms. Methods Twenty - four C57BL /6 mice were randomly divided into 3 groups: sham - ope...Objective To explore protective effect of hydrogen - rich saline on liver ischemia reperfusion ( IR) in mice and possible mechanisms. Methods Twenty - four C57BL /6 mice were randomly divided into 3 groups: sham - operated group,control group ( mice were injec-展开更多
Desorption of total saturated fractions(i.e. SAT, defined for this study as the summation of the concentrations of the saturated hydrocarbon from n-C10 to n-C26) and polycyclic aromatic fractions(i.e. PAH, defined as ...Desorption of total saturated fractions(i.e. SAT, defined for this study as the summation of the concentrations of the saturated hydrocarbon from n-C10 to n-C26) and polycyclic aromatic fractions(i.e. PAH, defined as the summation of the concentrations of all polycyclic aromatic fractions including the 16 EPA priority PAH) in two types of soils subjected to the changes of p H and salinity and different bio-surfactant concentrations were investigated. In general, compared with the experiments without bio-surfactant addition, adding rhamnolipid to crude oil-water systems at concentrations above its critical micelle concentration(CMC) values benefits SAT and PAH desorption. The results indicate that the change of p H could have distinct effects on rhamnolipid performance concerning its own micelle structure and soil properties. For loam soil, the adsorption of non-aqueous phase liquid(NAPL) and rhamnolipid would be the principle limiting factors during the NAPL removal procedure. For sand soil, less amount of rhamnolipid is adsorbed onto soil. Thus, with the increase of salinity, the solubilization and desorption of rhamnolipid solution are more significant. In summary, the p H and salt sensitivity of the bio-surfactant will vary according to the specific structure of the surfactant characteristics and soil properties.展开更多
Assuming a reservoir with a typical salt-lake background in the Qaidam Basin as a testbed,in this study the var-iation law of the rock electrical parameters has been determined through water displacement experiments w...Assuming a reservoir with a typical salt-lake background in the Qaidam Basin as a testbed,in this study the var-iation law of the rock electrical parameters has been determined through water displacement experiments with different salinities.As made evident by the results,the saturation index increases with the degree of water injec-tion.When the salinity of the injected water is lower than 80000 ppm,the resistivity of the rock samplefirst decreases,then it remains almost constant in an intermediate stage,andfinally it grows,thereby giving rise to a‘U’profile behavior.As the salinity decreases,the water saturation corresponding to the inflection point of the resistivity becomes lower,thereby leading to a wider‘U’type range and a higher terminal resistivity.For dif-ferent samples,higher initial resistivity of the sample in the oil-bearing state,and higher resistivity after low-sali-nity water washing are obtained when a thicker lithology is considered.展开更多
To ensure a safe and stable CO_(2)storage,pressure responses at an observation well are expected to be an important and useful field monitoring item to estimate the CO_(2)storage behaviors and the aquifer parameters d...To ensure a safe and stable CO_(2)storage,pressure responses at an observation well are expected to be an important and useful field monitoring item to estimate the CO_(2)storage behaviors and the aquifer parameters during and after injecting CO_(2),because it can detect whether the injected CO_(2)leaks to the ground surface or the bottom of the sea.In this study,pressure responses were simulated to present design factors such as well location and pressure transmitter of the observation well.Numerical simulations on the pressure response and the time-delay from pressure build-up after CO_(2)injection were conducted by considering aquifer parameters and distance from the CO_(2)injection well to an observation well.The measurement resolution of a pressure transmitter installed in the observation well was presented based on numerical simulation results of the pressure response against pressure build-up at the injection well and CO_(2)plume front propagations.Furthermore,the pressure response at an observation well was estimated by comparing the numerical simulation results with the curve of CO_(2)saturation and relative permeability.It was also suggested that the analytical solution can be used for the analysis of the pressure response tendency using pressure build-up and dimensionless parameters of hydraulic diffusivity.Thus,a criterion was established for selecting a pressure transducer installed at an observation well to monitor the pressure responses with sufficient accuracy and resolution,considering the distance from the injection well and the pressure build-up at the injection well,for future carbon capture and storage(CCS)projects.展开更多
Background Molecular hydrogen, as a novel antioxidant, has been proven effective in treating many diseases. This study aimed to evaluate the therapeutic effects of hydrogen saturated saline in treatment of a rat mode...Background Molecular hydrogen, as a novel antioxidant, has been proven effective in treating many diseases. This study aimed to evaluate the therapeutic effects of hydrogen saturated saline in treatment of a rat model of diabetes mellitus and a rat model of insulin resistant. Methods A rat diabetes mellitus model was established by feeding a high fat/high carbohydrate diet followed by injection of a small dose of streptozotocin, and an insulin resistant model was induced with a high glucose and high fat diet. Hydrogen saturated saline was administered to rats with both models conditions on a daily basis for eight weeks. A pioglitazone-treated group and normal saline-treated group served as positive and negative controls. The general condition, body weight, blood glucose, blood lipids, and serum insulin levels of rats were examined at the 8th week after treatment. The oxidative stress indices, including serum superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) were also evaluated after eight weeks of treatment using the commercial kits.Results Hydrogen saturated saline showed great efficiency in improving the insulin sensitivity and lowering blood glucose and lipids. Meanwhile, the therapeutic effects of hydrogen saturated saline were superior to those of pioglitazone. Hydrogen saturated saline markedly attenuated the MDA level and elevated the levels of antioxidants SOD and GSH. Conclusion Hydrogen saturated saline may improve the insulin resistance and alleviate the symptoms of diabetes mellitus by reducing the oxidative stress and enhancing the anti-oxidant system.展开更多
Soil salinization is a major problem affecting soils and threatening agricultural sustainability in arid and semi-arid regions,which makes it necessary to establish an efficient strategy to manage soil salinity and co...Soil salinization is a major problem affecting soils and threatening agricultural sustainability in arid and semi-arid regions,which makes it necessary to establish an efficient strategy to manage soil salinity and confront economic challenges that arise from it.Saline soil recovery involving drainage of shallow saline groundwater and the removal of soil salts by natural rainfall or by irrigation are good strategies for the reclamation of salty soil.To develop suitable management strategies for salty soil reclamation,it is essential to improve soil salinity assessment pro cess/mechanism and to adopt new approaches and techniques.T his study mapped a recovered area of 7200 m2 to assess and verify variations in soil salinity in space and time in K airouan region in Central Tunisia,taking into account the thickness of soil materials.Two electromagnetic conductivity meters(EM38 and EM31)were used to measure the electrical conductivity of saturated soil-paste extract(ECe)and apparent electrical conductivity(E Ca).Multiple linear regression was established between ECe and ECa,and it was revealed that ECa-EM38 is optimal for E Ce prediction in the surface soils.Salinity maps demonstrated that the spatial structure of soil salinity in the region of interest was relatively unchanged but varied temporally.Variation in salinity at the soil surface was greater than that at a depth.These findings can not only be used to track soil salinity variations and their significance in the field but also help to identify the spatial and temporal features of soil salinity,thus improving the efficiency of soil management.展开更多
The rate of evaporation of seawater droplets in the airsea boundary layer can be estimated by its salinity change compared to the sea surface salinity. A micro-chemical method based on Farlow (1954) is developed for q...The rate of evaporation of seawater droplets in the airsea boundary layer can be estimated by its salinity change compared to the sea surface salinity. A micro-chemical method based on Farlow (1954) is developed for quantitative determination of the salinity of an individual droplet without the error due to further evaporation after sampling. A halide ion-sensitive sampling surface is prepared by colloidally dispersing brown silver dichromate into the gelatin layer of a commercially available film. The reaction of soluble chlorides with the silver salt leaves a clear white halo with diameter a on the brown film to reveal the volume of the droplet- After the film is developed in a water vapor saturated atmosphere, the halo grows to diameter d with the original as the embryo to indicate the quantity of chloride ion involved in the action. The ratio of d/(a ) is a function of the salinity of the droplet. The method can be used to determine the salinity of seawater droplet of 10 to 10 ~10 gram. Details of展开更多
基金Supported by NFSC grant(30600700,30772413)Chinese PLA 12th five medical research grant
文摘Objective To study effects of saturated hydrogen saline in preventing noise-induced hearing loss. Methods Fifteen guinea pigs were randomly divided into 3 groups (5 each), group one was for control, group two was treated with normal saline and group three was treated with saturated hydrogen saline, which was given intraperitoneally at 1 hour before noise exposure at 1 ml/100 g. One hundred rounds of impulse noise ( 157 dB SPL peak) were delivered as noise exposure. Immediately after exposure to impulse noise and on Days 1, 2, 4 and 8 following exposure, auditory brainstem response (ABR) thresholds were measured. Outer hair cell morphological changes and sueeinate dehydrogenase (SDH) activity were examined on Day 8 post-exposure. Results Immediately after noise exposure, ABR thresholds in saturated hydrogen saline treated animals were lower than the non-treated animals (P 〈 0.05 ). Microscopy showed little SDH staining, cell swelling and irregular cell arrangement in the non-treated or normal saline treated animals. Whereas in the saturated hydrogen saline treated animals, there was deep SDH staining with significantly reduced cell loss and more regular cellular arrangement compared to the other two groups. The surviving cells counts was 45.17 ±12.15 for non-treated animals, 44.50 ±10.02 for normal saline treated animals and,116.50±2.38 for animals treated with saturated hydrogen saline. While the count was similar between non-treated and normal saline treated animals, it was significantly higher in saturated sions Intraperitoneal injection of saturated hydrogen saline damage. hydrogen saline treated animals (P 〈 0.05). Concluappears to protect the cochlea against noise-induced damage.
基金supported by grants from the National Natural Science Foundation of China(31472245 and 31772807)
文摘Background: Hydrogen-rich saline(HRS) has antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hydrogen on hepatic ischemia-reperfusion(I/R) and laparoscopic hepatectomy in swine. Methods: Twenty-one healthy Bama miniature pigs were randomly divided into the sham group, ischemia-reperfusion injury(IRI) group, HRS-5(5 m L/kg) group, and HRS-10(10 m L/kg) group. HRS was injected through the portal vein 10 min before reperfusion and at postoperative day 1, 2 and 3. The roles of HRS on oxidative stress, inflammatory response and liver regeneration were studied. Results: Compared with the IRI group, HRS treatment attenuated oxidative stress by increasing catalase activity and reducing myeloperoxidase. White blood cells in the HRS-10 group were reduced compared with the IRI group( P < 0.01). In the HRS-10 group, interleukin-1 beta, interleukin-6 and tumor necrosis factor alpha, C-reactive protein and cortisol were downregulated, whereas interleukin-10 was upregulated. In addition, HRS attenuated endothelial cell injury and promoted the secretion of angiogenic cytokines, including vascular endothelial growth factor, angiopoietin-1 and angiopoietin-2. HRS elevated the levels of hepatocyte growth factor, Cyclin D1, proliferating cell nuclear antigen, Ki-67 and reduced the secretion of transforming growth factor-beta. Conclusions: HRS treatment may exert a protective effect against I/R and hepatectomy-induced hepatic damage by reducing oxidative stress, suppressing the inflammatory response and promoting liver regeneration.
基金supported by a grant from Hunan Provincial Science and Technology Ministry of China,No.2015JJ6116
文摘Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspa se-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.
文摘Exposure of skin to solar ultraviolet(UV) radiation induces photo-damage.Ultraviolet B(UVB) is the major component of UV radiation which induces the production of reactive oxygen species(ROS) and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation.UVB radiation was irradiated on female C57BL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVB radia-tion had a significant affection in tumor necrosis factor alpha,interleukin(IL)-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVB radiation injury,possibly by reducing inflammation and oxidative stress.
基金Supported by the Natural Science Foundation of China(No.81300836)the Foundation of Open Sharing Platform of Science and Technology of Shaanxi Province,China(No.2015FWPT-02)
文摘AIM:To investigate the effects of hydrogen-rich saline(HRS)on microglia activation and Sirtuin type 1(Sirt1)in rats with N-methyl-N-nitrosourea(MNU)-induced retinitis pigmentosa(RP).METHODS:Rats were divided into norm(N)group,model(M)group and HRS(H)group.Rats in M and H groups were given saline and HRS respectively prior to and after administration of MNU.At one day(d1)and d3 afterwards,electroretinogram and histological examination were performed to confirm the effects of HRS on retinal function and structure of MNU-induced RP.Immunofluorescence staining of anti-ionized calcium-binding adapter molecule 1(Iba1),a maker of microglia cells,was performed,with quantitative real-time polymerase chain reaction(qRT-PCR)for its m RNA quantification.Moreover,Sirt1 m RNA and protein expression in the retinas were detected by Western blot and qRT-PCR.RESULTS:HRS preserved the retinal function and mitigated the reduction of photoreceptor degeneration in MNU-treated retinas.The presence of microglia cells was somewhat more obvious in H group than that in M group at d1.HRS suppressed the further activation of microglia cells,with the number of microglia cells less than that of M group at d3.Results of qRT-PCR of Iba1 were consistent with those of immunofluorescence staining,with the m RNA expression of Iba1 in H group more intensive than that of M group at d1(P〈0.05),while less than that of M group at d3(P〈0.05).Furthermore,the Sirt1 m RNA and protein expression decreased after MNU administration,while HRS mitigated the MNU-induced downregulation of Sirt1.CONCLUSION:HRS can effectively keep microglia activation induced by MNU to an appropriate extent,while upregulate Sirt1 in MNU-induced RP.
文摘Objective To explore protective effect of hydrogen - rich saline on liver ischemia reperfusion ( IR) in mice and possible mechanisms. Methods Twenty - four C57BL /6 mice were randomly divided into 3 groups: sham - operated group,control group ( mice were injec-
基金Project(8102032) supported by Beijing Natural Science Foundation of China
文摘Desorption of total saturated fractions(i.e. SAT, defined for this study as the summation of the concentrations of the saturated hydrocarbon from n-C10 to n-C26) and polycyclic aromatic fractions(i.e. PAH, defined as the summation of the concentrations of all polycyclic aromatic fractions including the 16 EPA priority PAH) in two types of soils subjected to the changes of p H and salinity and different bio-surfactant concentrations were investigated. In general, compared with the experiments without bio-surfactant addition, adding rhamnolipid to crude oil-water systems at concentrations above its critical micelle concentration(CMC) values benefits SAT and PAH desorption. The results indicate that the change of p H could have distinct effects on rhamnolipid performance concerning its own micelle structure and soil properties. For loam soil, the adsorption of non-aqueous phase liquid(NAPL) and rhamnolipid would be the principle limiting factors during the NAPL removal procedure. For sand soil, less amount of rhamnolipid is adsorbed onto soil. Thus, with the increase of salinity, the solubilization and desorption of rhamnolipid solution are more significant. In summary, the p H and salt sensitivity of the bio-surfactant will vary according to the specific structure of the surfactant characteristics and soil properties.
基金The authors would like to acknowledge the financial support from the Jiangsu Key Laboratory of Oil-Gas Storage and Transportation Technology(Grant No.CDYQCY202201)funded by the Key Laboratory of Shallow Geothermal Energy,Ministry of Natural Resources of the People’s Republic of China.The authors thank the anonymous reviewers for their constructive and valuable opinions gratefully.
文摘Assuming a reservoir with a typical salt-lake background in the Qaidam Basin as a testbed,in this study the var-iation law of the rock electrical parameters has been determined through water displacement experiments with different salinities.As made evident by the results,the saturation index increases with the degree of water injec-tion.When the salinity of the injected water is lower than 80000 ppm,the resistivity of the rock samplefirst decreases,then it remains almost constant in an intermediate stage,andfinally it grows,thereby giving rise to a‘U’profile behavior.As the salinity decreases,the water saturation corresponding to the inflection point of the resistivity becomes lower,thereby leading to a wider‘U’type range and a higher terminal resistivity.For dif-ferent samples,higher initial resistivity of the sample in the oil-bearing state,and higher resistivity after low-sali-nity water washing are obtained when a thicker lithology is considered.
基金We acknowledge the funding support from the Research Fund for the special projects in key fields of Guangdong Universities(Grant No.2021ZDZX4074)the Japan Society for the Promotion of Science(Grant No.JP-20K21163)Scientific Research Fund of Hainan University(Approval No.KYQD(ZR)-22122).
文摘To ensure a safe and stable CO_(2)storage,pressure responses at an observation well are expected to be an important and useful field monitoring item to estimate the CO_(2)storage behaviors and the aquifer parameters during and after injecting CO_(2),because it can detect whether the injected CO_(2)leaks to the ground surface or the bottom of the sea.In this study,pressure responses were simulated to present design factors such as well location and pressure transmitter of the observation well.Numerical simulations on the pressure response and the time-delay from pressure build-up after CO_(2)injection were conducted by considering aquifer parameters and distance from the CO_(2)injection well to an observation well.The measurement resolution of a pressure transmitter installed in the observation well was presented based on numerical simulation results of the pressure response against pressure build-up at the injection well and CO_(2)plume front propagations.Furthermore,the pressure response at an observation well was estimated by comparing the numerical simulation results with the curve of CO_(2)saturation and relative permeability.It was also suggested that the analytical solution can be used for the analysis of the pressure response tendency using pressure build-up and dimensionless parameters of hydraulic diffusivity.Thus,a criterion was established for selecting a pressure transducer installed at an observation well to monitor the pressure responses with sufficient accuracy and resolution,considering the distance from the injection well and the pressure build-up at the injection well,for future carbon capture and storage(CCS)projects.
基金This study was supported by the Natural Science Foundation of Shanghai (No. 09ZR1400700).
文摘Background Molecular hydrogen, as a novel antioxidant, has been proven effective in treating many diseases. This study aimed to evaluate the therapeutic effects of hydrogen saturated saline in treatment of a rat model of diabetes mellitus and a rat model of insulin resistant. Methods A rat diabetes mellitus model was established by feeding a high fat/high carbohydrate diet followed by injection of a small dose of streptozotocin, and an insulin resistant model was induced with a high glucose and high fat diet. Hydrogen saturated saline was administered to rats with both models conditions on a daily basis for eight weeks. A pioglitazone-treated group and normal saline-treated group served as positive and negative controls. The general condition, body weight, blood glucose, blood lipids, and serum insulin levels of rats were examined at the 8th week after treatment. The oxidative stress indices, including serum superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) were also evaluated after eight weeks of treatment using the commercial kits.Results Hydrogen saturated saline showed great efficiency in improving the insulin sensitivity and lowering blood glucose and lipids. Meanwhile, the therapeutic effects of hydrogen saturated saline were superior to those of pioglitazone. Hydrogen saturated saline markedly attenuated the MDA level and elevated the levels of antioxidants SOD and GSH. Conclusion Hydrogen saturated saline may improve the insulin resistance and alleviate the symptoms of diabetes mellitus by reducing the oxidative stress and enhancing the anti-oxidant system.
文摘Soil salinization is a major problem affecting soils and threatening agricultural sustainability in arid and semi-arid regions,which makes it necessary to establish an efficient strategy to manage soil salinity and confront economic challenges that arise from it.Saline soil recovery involving drainage of shallow saline groundwater and the removal of soil salts by natural rainfall or by irrigation are good strategies for the reclamation of salty soil.To develop suitable management strategies for salty soil reclamation,it is essential to improve soil salinity assessment pro cess/mechanism and to adopt new approaches and techniques.T his study mapped a recovered area of 7200 m2 to assess and verify variations in soil salinity in space and time in K airouan region in Central Tunisia,taking into account the thickness of soil materials.Two electromagnetic conductivity meters(EM38 and EM31)were used to measure the electrical conductivity of saturated soil-paste extract(ECe)and apparent electrical conductivity(E Ca).Multiple linear regression was established between ECe and ECa,and it was revealed that ECa-EM38 is optimal for E Ce prediction in the surface soils.Salinity maps demonstrated that the spatial structure of soil salinity in the region of interest was relatively unchanged but varied temporally.Variation in salinity at the soil surface was greater than that at a depth.These findings can not only be used to track soil salinity variations and their significance in the field but also help to identify the spatial and temporal features of soil salinity,thus improving the efficiency of soil management.
基金The research was supported by the Chinese National Natural Science Foundation
文摘The rate of evaporation of seawater droplets in the airsea boundary layer can be estimated by its salinity change compared to the sea surface salinity. A micro-chemical method based on Farlow (1954) is developed for quantitative determination of the salinity of an individual droplet without the error due to further evaporation after sampling. A halide ion-sensitive sampling surface is prepared by colloidally dispersing brown silver dichromate into the gelatin layer of a commercially available film. The reaction of soluble chlorides with the silver salt leaves a clear white halo with diameter a on the brown film to reveal the volume of the droplet- After the film is developed in a water vapor saturated atmosphere, the halo grows to diameter d with the original as the embryo to indicate the quantity of chloride ion involved in the action. The ratio of d/(a ) is a function of the salinity of the droplet. The method can be used to determine the salinity of seawater droplet of 10 to 10 ~10 gram. Details of